Growth Of Plantlets Research Articles

In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

Plant genetic resources conservation may be a potential option for the improvement of agricultural crops through modern biotechnologies, and in vitro conservation is a tool available to safeguard plant biodiversity. Ex situ conservation of plant genetic resources using the in vitro procedures is in progress in many countries. The slow growth storage (SGS) technique is a valid in vitro approach to preserve several vegetatively propagated species by controlling the growth and development of plantlets, economizing storage space and labor and reducing costs. Moreover, SGS prolongs the timing between subcultures, lowers the risk of losing germplasm through handling errors, such as contamination problems, and decreases the risk of genetic instability due to the reduction in the number of subcultures. SGS is applied by considering different factors: temperature, light or darkness conditions, medium composition, including mineral or sucrose concentrations, and the presence/absence of plant growth regulators, osmotic agents and growth inhibitors. SGS protocols for some fruit species have been well defined, others require additional research. The present review focuses on the effect of several factors that influence the SGS of in vitro shoots derived from temperate and tropical fruit species during the last ten years.

Cutting Efficiency of a Newly Developed Incubator-Based Laser Cutting Method and Its Effects on Potato Tissue Culture Plantlet Growth

Cutting Efficiency of a Newly Developed Incubator-Based Laser Cutting Method and Its Effects on Potato Tissue Culture Plantlet Growth

Biostimulation can prime elicitor induced resistance of grapevine leaves to downy mildew.

Using plant defense elicitors to protect crops against diseases is an attractive strategy to reduce chemical pesticide use. However, development of elicitors remains limited because of variable effectiveness in the field. In contrast to fungicides that directly target pathogens, elicitors activate plant immunity, which depends on plant physiological status. Other products, the biostimulants, can improve certain functions of plants. In this study, the objective was to determine whether a biostimulant via effects on grapevine physiology could increase effectiveness of a defense elicitor. A new methodology was developed to study biostimulant activity under controlled conditions using in vitro plantlets. Both biostimulant and defense elicitor used in the study were plant extracts. When added to the culture medium, the biostimulant accelerated the beginning of plantlet growth and affected the shoot and root development. It also modified metabolomes and phytohormone contents of leaves, stems, and roots. When applied on shoots, the defense elicitor changed metabolite and phytohormone contents, but effects were different depending on whether plantlets were biostimulated or controls. Defense responses and protection against Plasmopara viticola (downy mildew agent) were induced only for plantlets previously treated with the biostimulant, Therefore, the biostimulant may act by priming the defense elicitor action. In this study, a new method to screen biostimulants active on grapevine vegetative growth was used to demonstrate that a biostimulant can optimize the efficiency of a plant defense elicitor.

Rhizome Weight and Number of Sectioning per Rhizome Determine Plantlet Growth and Propagation Rate of Hemerocallis citrina Baroni in Cutting Propagation

Rhizome cutting is prevalent in propagation for Hemerocallis citrina Baroni. This study aimed to reveal the influence of rhizome weight on plantlet growth, and evaluate a new cutting protocol. Three levels of rhizome weight (big (BR), medium (MR) and small (SR)) were compared by measuring plantlet growth four times during cutting propagation. Sectioning rhizomes longitudinally along shaft axis of crown into 2, 3 or 4 parts (S2, S3 and S4), was tested on rhizomes with two bud sizes (Large bud (LB), Small bud (SB)). Propagation coefficient, the number of plantlets obtained per rhizome, kept similar regardless of rhizome weight, while S3 and S4 increased propagation coefficient. Rhizome weight changed the relative growth rates on different dates. SR resulted in lower aboveground dry biomass, leaf area, total fibrous root length, relative total fibrous root length growth rate and N deficiency compared to BR. Sucrose concentration in rhizome decreased with time and fructose concentration was lowest in MR at the end of experiment. Aboveground dry biomass and total fibrous root length were lowest in S4, followed by S3 and S2. Rhizome weight and number of sectioning per rhizome influence cutting propagation, and sectioning rhizomes into three pieces has higher propagation coefficient and less retardation on growth. According to N deficiency in plantlets during the propagation, N fertiliser is probably needed during cutting.

Growth of Potato Plantlets in Response to Ventilation, Sucrose, and Paclobutrazol in Modified Temporary Immersion Vessels

This study investigated the effects of different sucrose, ventilation, and paclobutrazol (PBZ) treatments on the growth of potato plantlets cultured in modified temporary immersion culture vessels. Temporary immersion culture vessels were modified to enable the plantlets to be cultured on semi-solid growth medium and provided with forced ventilation. The plantlet responses to two sucrose concentrations (15 g⋅L−1 and 30 g⋅L−1) in combination with two forced ventilation treatments (5 min/2 h and no ventilation) and three PBZ concentrations (0, 0.2, and 0.4 mg⋅L−1) were studied. Shoot growth was severely inhibited by PBZ in the growth media, whereas leaf formation was promoted by ventilation irrespective of the PBZ concentration. In nonventilated vessels, it is noteworthy that PBZ was able to increase the number of leaves formed in plantlets grown on medium supplemented with 30 g⋅L−1 sucrose, but not on medium with 15 g⋅L−1 sucrose. In growth media without PBZ, a high percentage of potato plantlets was able to produce secondary axillary shoots when provided with 30 g⋅L−1 sucrose. However, their ability to produce secondary shoots was reduced when PBZ was included in the growth medium, particularly those with 15 g⋅L−1 sucrose. Plantlets grown in ventilated culture vessels combined with 30 g⋅L−1 sucrose in the growth medium produced more than three times more shoots than the other treatments. Under ex vitro conditions, plantlets that had been grown in ventilated vessels had fewer leaf deaths, and the inclusion of PBZ in the growth media further reduced the number of dead leaves. Findings of this study showed that PBZ had a key role in the responses of potato plantlets to ventilation and sucrose treatments, as well as their tolerance to ex vitro conditions. The modified temporary immersion system can be used for the in vitro culture of potato plantlets on semi-solid medium and provide forced ventilation to improve their growth.

Effect Of Indole Butyric Acid (Iba) And Thidiazuron (Tdz) Concentrations On Explant Growth Planlet Porang (Amorphophallus Muelleri Blume.) In Vitro Culture

Porang (Amorphophallus muelleri Blume.) is a type of tuber plant that is widely available in Indonesia and has tremendous potential and prospects to be developed in Indonesia. Porang is widely exported from Indonesia to several countries. The demand for porang exports continues to increase while the production of porang is still low. In vitro culture is an alternative solution in porang plant propagation to meet the needs of many porang seeds and in a relatively short period of time. Provision of growth regulators in the media can affect the growth of porang. This study aims to obtain information whether there is an interaction between IBA and TDZ in the media, to obtain information on the effect of adding IBA and TDZ with different concentrations on the growth of porang plantlets. This research was conducted using MS medium which was then added with IBA with a concentration of 0.5 ppm, 1 ppm and added ZPT TDZ with a concentration of 0.5 ppm, 1 ppm and 1.5 ppm. The results of this study showed that there was no significant difference between the emergence of shoots and the time of emergence of leaves from all treatments. The I1T3 treatment (IBA 0.5 ppm + TDZ 1.5 ppm) was the best combination treatment in this study by showing the fastest root emergence time (6 MSI), the highest average number of shoots (7.25 shoots), the average number of shoots the highest number of roots (3.67 roots), the highest average number of leaves (6 strands) and the highest average shoot height (1.33 cm).

Physiological Response, Oxidative Stress Assessment and Aquaporin Genes Expression of Cherry Tomato (Solanum lycopersicum L.) Exposed to Hyper-Harmonized Fullerene Water Complex.

The rapid production and numerous applications of nanomaterials warrant the necessity and importance of examining nanoparticles in terms to their environmental and biological effects and implications. In this study, the effects of a water-soluble hyper-harmonized hydroxyl-modified fullerene (3HFWC) on cherry tomato seed germination, seedlings growth, physiological response and fruiting was evaluated. Changes in the photosynthetic pigments content, oxidative stress assessment, and aquaporin genes expression in cherry tomato plants were studied after during short- and long-term continuous exposure to 3HFWC nanosubstance (200 mg/L). Increased levels of photosynthetic pigments in leaves, lycopene in fruits, decreased levels of hydrogen peroxide content, activation of cellular antioxidant enzymes such as superoxide dismutase, catalase and peroxidase and increased aquaporin gene expression (PIP1;3, PIP1;5 and PIP2;4) were observed in 3HFWC nanosubstance-exposed plants in comparison to control, untreated cherry tomato plants. The 3HFWC nanosubstance showed positive effects on cherry tomato seed germination, plantlet growth and lycopene content in fruits and may be considered as a promising nanofertilizer.

<i>In Vitro</i> Culture of <i>Phalaenopsis amabilis</i> (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for <i>Ex Situ</i> Conservation

In vitro culture is one of the effective cultivation methods for seedling production of orchids that can be used as a powerful tool in the conversation of orchids. The aims of the present study were to (i) investigate the effects of the addition of banana extract (0 g/l, 100 g/l, and 150 g/l) on media (NP media), in two different light regimes (light and dark conditions) on the growth of plantlets of an epiphytic orchid Phalaenopsis amabilis in in vitro culture. Methods used included (i) subculturing orchid seedlings in treatments media, (ii) measuring leaves and roots chlorophyll content and growth parameters, (iii) anatomical preparation of leaves and roots of the seedlings. The results showed that the best condition for getting greater seedlings of P. amabilis plantlets is in media with an addition of 100 g/L banana extract in light condition. The highest amount of chlorophyll in the P. amabilis leaves was found in medium with the addition of 100 g/L banana extract medium in light conditions. The thickness of mesophyll and the largest root diameter of P. amabilis seedlings were also found in media with the addition of 100 g/L banana extract medium in light condition. In conclusion, the addition of 100gr/L banana extract into basic culture medium will be beneficial for seedlings production of P. amabilis with great appearance, for ex situ orchid conservation programs.

Effect of vermicompost on vegetative growth and nutrient status of acclimatized Grand Naine banana plants

Novel approach for introducing vermicompost as an acclimatization soil for banana plants was conducted. Different potting mixture of vermicompost, vermiculite, peatmoss and sand at different ratios were used to study the effect of different agricultural media on vegetative growth and leaf mineral content of banana tissue culture plantlets, after acclimatization stage. Two different trial periods were studied; 12 and 24 weeks in these agricultural media for two seasons. Results indicated that using vermicompost combined with vermiculite and sand (33.3% for each) recorded the best results for the most vegetative growth studied parameters (plant height, plants and roots length, stem diameter, leaf width and shoot and root fresh and dry weight). Moreover, vermicompost at 50% + peat moss at 50% significantly increased total chlorophyll content in banana plants. Furthermore, N, P and K plant analysis has shown that vermicompost at 75% with peat moss gave the highest mineral content values at the two periods. Addition of vermicompost to the culture media improved in vitro-produced banana plants in greenhouse.

Cryopreservation of coconut plumule using droplet vitrification

In the present investigation, four types of explants from mature zygotic embryos of coconut, viz., whole upper cotyledonary region without haustorium, half of the upper cotyledonary region without haustorium, plumule with a portion of radicle and exclusively plumular tissue, were cultured in 12 different media combinations to find a suitable explant which could be regenerated after cryopreservation. Explants were pre-cultured in medium with 0.4 and 0.5 M sucrose for three days followed by dehydration in PVS3 solution for different durations on a sterile aluminum strip after treating with loading solution. Strips were treated with liquid nitrogen inside a cryoflask until bubbling stopped and quickly transferred to a cryovial and stored for a minimum period of 24 hours in liquid nitrogen. It was observed that plumule alone or with a small portion of outer tissue was ideal for fast in vitro growth and recovery of whole plantlets of coconut in a medium supplemented with NAA alone. Addition of glutamine (5 mg L-1), TDZ (1 mg L-1) and NAA (18 mg L-1) aided the vigorous growth of plantlets. In control, the survival rate ranged from 60 to 90 per cent in plumule pre-grown in media containing 0.5 M sucrose after dehydration with PVS3 for various durations, whereas it was 14 to 75 per cent in cryopreserved ones. Considering the high survival (75%) and regrowth (35%) of cryopreserved plumule in the present study, there is much scope for further improvement of the procedure to find the right combination of factors so as to enhance complete recovery of plantlets without much injury to plumules during cooling and rewarming.

Establishment of an acclimatization protocol for pepper (Piper nigrum L.) plantlets post-in vitro stage

This study was conducted with the aim of determining the factors that affect the growth and development of in vitro-derived pepper plantlets variety Vinh Linh in greenhouse. The results indicated that gradual adaptation of in vitro plantlets to ex vitro environment by placing enclosed plantlets-containing culture vessels in greenhouse for 2 weeks improved the survival and growth of pepper plantlets upon transfer to acclimatization substrate. The plantlets were required to reach the weight of 0.7-0.9 g before transplanting into nursery bags containing substrate that was red soil:cow dung:coconut husk:rice husk (5:2:2:1). Alternative application of NPK 0.2% and foliar fertilizer Nupe 0.2% every 2 weeks positively affected plant growth and the plants were ready for transplanting into the field after about 5 months. The research results establish an acclimatization protocol for pepper plantlets post-in vitro stage that helps to complete the protocol for pepper production using micropropagation method.

Aklimatization Black Orchid Plantlets (Coelogyne pandurata Lindl.) with Biostimulant Moringa Leaf Extract (Moringa oleifera Lamk.)

The growth of black orchid plantlets (Coelogyne pandurata Lindl.) are slow at the acclimatization stage. An alternative was used moringa leaf extract (Moringa oleifera Lamk.) as a biostimulant. The aim of this research to find out the effect of giving moringa leaf extract on the growth of black orchid plantlets at the acclimatization stage and to obtain the best concentration and timing of extract to increase the growth of black orchid plantlets. The research used a factorial completely randomized design (FCRD) method with two factors. Factor I is the concentration of the extract which consists of 5 levels, namely 0, 25, 50, 75 and 100 mg/L. Factor II is the application time which consists of 2 levels, namely once a week and every 2 weeks, each treatment is repeated 5 times. The results showed that moringa leaf extract had a significant effect on the mean leaf width and number of plantlet roots of black orchids. Extract concentration of 50 mg/L was the best concentration on the growth of black orchid plantlets with an average leaf width of 0.76 cm and an average root number of 8.2. Recommended for the acclimatization of black orchids to use extract concentration 50 mg/L.

Different types of explants and natural ventilation systems influence the accumulation of dry weight and of total phenolic compounds in Aloysia gratissima (Verbenaceae)

The objective of this study was to evaluate the effect of the use of different natural ventilation systems on the growth and accumulation of total phenolic compounds in Aloysia gratissima. Nodal and apical segments with 1 pair of leaves were inoculated in flasks containing ½ MS culture medium without sucrose and supplemented with 0.1 mg L-1 indole-butyric acid. The cultivation systems included a conventional system (NMS) and alternative membrane systems with 1 (AMS1), 2 (AMS2) and 4 (AMS4) low-cost membranes. After 30 days, the in vitro growth, leaf area, dry weight and accumulation of phenolic compounds were evaluated. Plantlets from apical segments showed superior results in all variables analyzed. The use of the NMS compromised plantlet growth. However, improved results were obtained with the use of porous membranes, with the best growth observed in AMS4. The leaf area of plantlets originating from apical segments was 3.03 times greater than that of plantlets from nodal segments. Plantlets in the NMS had the lowest values of leaf area, root length, number of leaves and dry weight. However, the use of membranes allowed higher growth. The AMS4 treatment increased the leaf dry weight accumulation by 6.13-fold compared to that obtained with the NMS treatment. The accumulation of total phenolic compounds increased with the use of greater numbers of porous membranes. The use of apical segments and lids with 4 porous membranes is recommended for micropropagation of the species. The use of an alternative membrane system positively influences the accumulation of total phenolic compounds.

Arbuscular mycorrhizal fungi enhanced the growth, phosphorus uptake and Pht expression of olive (Olea europaea L.) plantlets.

Olive (Olea europaea L.) is a highly mycotrophic species that has been introduced and cultivated in China for half a century. The arbuscular mycorrhizal fungi (AMF) is extremely valuable as a kind of biofertilizer to promote the health and vigor of olive plants. However, it is still unclear how native AMF impact growth and mineral nutrients, especially phosphorus absorption in the area where olive trees were introduced in China. In the present study, through a pot experiment, the effects of native AMF on the growth, phosphorus uptake and expression levels of four phosphate transporter genes (Pht) of olive plantlets were characterized. We found that (1) typical AMF colonization was observed within the roots of inoculated olive plantlets, and the growth of plantlets was significantly promoted; (2) some indigenous consortia (AMF1 and AMF2) notably promoted the absorption of phosphorus, fertilizers significantly increased the foliar content of nitrogen, and both AMF inoculation and fertilization had no significant effect on the uptake of potassium; and (3) AMF inoculation enhanced the expression of phosphate transporter genes in inoculated olive roots. This work demonstrates the effectiveness of native AMF on the cultivation of robust olive plantlets and highlights the role of AMF in increasing phosphorus uptake. There is great potential in using native AMF consortia as inoculants for the production of healthy and robust olive plantlets.

Effect of Biodegradable Coatings on the Growth of Aspergillus flavus In Vitro, on Maize Grains, and on the Quality of Tortillas during Storage.

The fungus Aspergillus flavus causes serious damage to maize grains and its by-products, such as tortilla. Currently, animal and plant derivatives, such as chitosan and propolis, and plant extract residues, respectively, are employed as alternatives of synthetic fungicides. The objective of this research was to evaluate the efficacy of several formulations based on propolis-chitosan-pine resin extract on the in vitro growth of A. flavus, the growth of maize grain plantlets and the quality of stored tortillas at 4 and 28 °C. The most outstanding formulation was that based on 59.7% chitosan + 20% propolis nanoparticles + 20% pine resin extract nanoparticles; since the in vitro conidia germination of A. flavus did not occur, disease incidence on grains was 25–30% and in tortillas, 0% infection was recorded, along with low aflatoxin production (1.0 ppb). The grain germination and seedling growth were markedly reduced by the nanocoating application. The percentage weight loss and color of tortillas were more affected by this coating compared to the control, and the rollability fell within the scale of non-ruptured at 4 °C and partially ruptured at 28 °C. The next step is to evaluate the toxicity of this formulation.

The Acclimatization Stage Growth Of Black Orchid (Coelogyne Pandurata Lindl.) Plantlet By Biostimune Extract Gotu Kola (Centella Asiatica L.) Treatment

Black orchid plantlets have a relatively slow growth at the acclimatization stage. The growth of black orchid plantlets can be increased by giving biostimulants. Biostimulants are bioactive compounds, and when they are applied, they can increase plant growth. This study aims to determine the effect of biostimulant Gotu kola extract (Centella Asiatica L.) on the growth of black orchid plantlets (Coelogyne Pandurata Lindl.) at the acclimatization stage. The research was conducted at the Biology Department's greenhouse from September until December 2020. The study used a completely randomized design with a factorial pattern. The first factor is the concentration of biostimulants consisting of 5 levels, e.g: 0 mg/L; 25 mg/L; 50 mg/L; 75 mg/L; 100 mg/L. The second factor is the application time which consists of 2 levels: once a week and once every two weeks. The results showed that the application of biostimulant Gotu kola extract had no significant effect on plantlet height, leaf length, leaf width, number of leaves, number of tillers, and number of plantlet roots of black orchid in the acclimatization stage.

Analysis of Phenol, Reducing Sugar, and Chlorophyll of Dendrobium sp. plantlet after induction of fusaric acid In Vitro

Orchids are ornamental plants with varied and beautiful flower colors and are much favored by the public. Orchid plants have a higher economic value when compared to other ornamental plants, both as cut flowers and potted flowers. In the process of growth, orchid plants are disturbed, namely Fusarium wilt disease caused by Genus Fusarium which can produce a toxin compound, namely fusaric acid which is widely used in in vitro selection of plants. Plant resistance can be formed through the induction of fusaric acid compounds. Induced resistance is an alternative way to obtain disease-resistant plants because plants are able to create natural resistance mechanisms. The purpose of this study was to determine the concentration of tolerant fusaric acid to select Dendrobium orchid plantlets with optimal growth and specific expression characters of Dendrobium orchid plantlets based on phenol content, reducing sugar content, and total chlorophyll content, chlorophyll a and chlorophyll b. The results of this study indicated that there was an increase in total phenol content, reducing sugar content, and total chlorophyll content, chlorophyll a and chlorophyll b along with the addition of fusaric acid concentration.

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

In vitro propagation of Cavendish banana is an alternative to produce healthy, uniform and sufficient plant materials of high qualities. Appropriate culture media formulation is needed for shoot growth and rooting stage. This experiment aimed to study effects of IAA and organic supplements on in vitro shoot growth and rooting. Explants were subjected to MS medium with IAA and various organic supplements, in a factorial arrangement of a completely randomized design. The first factor was IAA concentrations (0; 0.5; 1 and 1.5 mg.L-1), and the second factor was types of organic supplement (control, coconut water, potato extract and banana extract). At 8 weeks after planting, shoot growth and root performance of plantlets were recorded. The results showed that addition of IAA did not affect shoot height. Addition of potato extract and banana homogenate resulted in the highest plantlets. Medium with IAA at 1 mg.L-1 resulted in the highest number of roots. The highest number of shoots was produced on media with IAA 1 mg.L-1 + coconut water and IAA 1.5 mg.L-1 + potato extract. The highest number of roots was produced in medium with potato extract. The longest roots were produced in media with 0.5 mg.L-1 IAA + banana homogenate. Keywords: Banana extract, Cavendish, Coconut water, IAA, Potato extract

Study on in vitro propagation of \(\textit{Vaccinium myrtillus}\) Linn. via nodal culture method

Vaccinium myrtillus Linn. is an imported fruit tree with high economic value. Currently, in Vietnam there has not been any publication on in vitro propagation of the blueberry plant. This study is focused on in vitro propagation of V. myrtillus by nodal culture. The results showed that in vitro shoot growth and proliferation were the best when nodes were cultured on WPM medium supplemented with 0.5 mg/L zeatin, 30 g/L sucrose, 10 g/L agar, pH 5.8 (shoot height 4.24 cm, 1 shoot/explant, 8.60 nodes/shoot). The third nodes to sixth nodes proved to be suitable materials for in vitro propagation of V. myrtillus. The proliferation and growth of shoots on the medium without activated charcoal (plant height 3.92 cm, 1 shoot/explant, 8.60 nodes/shoot) were better than those grown on the medium supplemented with 1 g/L of activated charcoal (plant height 3.64 cm, 1 shoot/explant, 7.90 nodes/shoot). For the in vitro rooting stage, the WPM medium containing 1.0 mg/L IBA, 30 g/L sucrose, 10 g/L agar, pH 5.8 resulted in plant height 5.49 cm, root length 1.42 cm, root formation rate 100%. After transferring the cultivated-tissue Vaccinium myrtillus to the greenhouse, substrate with coconut fibre powder, the results were observed to be plant height 6.94 cm, root length 3.18 cm, survival rate 95%. The plantlet growth increased overtime after 4 months in the greenhouse.

Metabolomics and transcriptomics analysis of vitro growth in pitaya plantlets with different LED Light spectra treatment

Dragon fruit or pitaya has important practical and economic value that is cultivated widely in the world. In order to meet the market demand for pitaya seedlings, vitro (tissue) culture is the most widely and practical technique, which cultivated in greenhouses and the intensity and quality of light is also important factor for plant photosynthesis. However, little information was obtained about the molecular and metabolite basis on the different light spectra (quality) cultivation conditions in pitaya seedlings. Therefore, metabolomics and transcriptomics analyses were conducted in this study, to explore the effects of different light quality and wavelength ratios on the growth of pitaya plantlets. The results have shown that different light spectra can significantly change the growth, physiological and morphological in pitaya plants. Red light significantly inhibited biomass, stem diameter, and cross-sectional area (CSA) of pitaya plantlets, while blue light can alleviate this inhibition, and 1 Red+ 2 Blue mixture light (1R2B) had the best effect on pitaya plants. Red light also promoted plant height, while blue light inhibited this phenomenon. In addition, the results also showed that the red light significantly influences primary metabolic processes in pitaya plantlets, including glucose metabolism and photosynthesis. And the expression of genes and accumulation of metabolites in these pathways were reduced by red light treatment, while blue light can change this negative pattern. Furthermore, the accumulation of sucrose, glucose, fructose, fructose-6-phosphate, fatty acid, and flavonoids were found to significantly increased in 1R2B treatment group of pitaya plantlets, which further regulation the tricarboxylic acid cycle (TCA cycle) pathway in the energy metabolism process. Therefore, the results suggested that the mixture light quality (1R2B) may be the best condition for the high-quality cultivation of pitaya seedlings in the present study.