This research aims to determine the growth response and yield of several Black Soybean varieties in several variations of spacing. The experiment is a factorial experiment using a randomized block design (RBD) consisting of two treatment factors, the first factor is the variety consisting of 3 levels: V1 = Detam 1, V2 = Detam 3 Prida, V3 = Detam 4 Prida. The second factor is spacing which consists of 3 levels: J1 = 20 cm x 20 cm, J2 = 20 cm x 30 cm, J3 = 20 cm x 40 cm. The interaction of varieties with plant spacing has a very significant effect on crop dry weight and seed oven per planting hole. The significant effect the yield seeds of dried and oven per hectare. The weight of oven-dry seeds per planting hole and the highest yield of oven-dried seeds per hectare was obtained in the interaction of Detam 1 varieties with the spacing of 20 cm x 40 cm with was 33.79 g and 2.53 tons per hectare which increased 138.79% and 42.93% compared with the interaction of Detam 4 Prida varieties with a spacing of 20 cm x 20 cm with was 14.15 g and 1.77 tons per hectare.