Riparian zones, acting as transitional areas between aquatic and terrestrial ecosystems, boast a rich diversity of plant species. However, alterations in river hydrological regimes can significantly impact plant growth and distribution. In this study, seven typical reaches of Yongding River Basin were selected, and xylem water, soil water at different depths, and river water were collected in May and August. By measuring δ2H and δ18O values and combining with MixSIAR model, the proportion of water utilization by plants from different sources was quantified. The findings revealed that δ2H and δ18O values of river water, soil water, and plant xylem water were higher in August compared to May. While there was no significant difference in δ2H and δ18O values between river and soil water during different periods (p > 0.05), significant differences were observed in δ18O in plant xylem water (p = 0.022). Regardless of whether it was May or August, herbaceous plants utilized river water more extensively than trees and shrubs, and hydrophytes exhibited a higher dependence on river water compared to mesophytes and xerophytes. Some hygrophytes (P. anserina, etc.) utilized river water for over 90% of their total water intake. There were significant differences between herbs and trees and shrubs in the proportion of river water usage in August (p = 0.001). Moreover, considerable variations existed in the proportion of river water usage among different water ecotypes in both May (p = 0.005) and August (p < 0.001). Our findings provide a scientific basis for the rational allocation of plants in the process of riparian vegetation restoration.
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