Maize Plants Research Articles

Exogenously applied ABA alleviates dysplasia of maize (Zea mays L.) ear under drought stress by altering photosynthesis and sucrose transport

ABSTRACT Drought stress inhibits the development of maize ears. Abscisic acid (ABA) is a plant hormone that can regulate the physicology metabolism under abiotic stress. In this study, maize varieties Zhengdan 958 (ZD958) and Xianyu 335 (XY335) with different filling stages were used as materials. Three treatments were set in the filling period: normal irrigation (CK), drought stress (stress); exogenous ABA + drought stress (ABA+stress). They were used to study the physiological regulation of exogenous ABA on maize ears development during drought stress. Exogenous ABA inhibited bald tip and the decline of maize plant biomass, and increased the number and weight of grains per ear at harvest under drought stress by regulating photosynthetic pigment content (Chla, Chlb, Car), gas exchange parameters (Pn, Tr, gs, Ci, Ls), Chla fluorescence parameters (Fv/Fm, ФPSII, ETR, qP, NPQ), chloroplast structure and function, photosynthetic enzyme activity, and the transcription level of genes coding SUTs (ZmSUT1, ZmSUT2, ZmSUT4, ZmSUT6). There was a significant correlation between physiological indexes of sucrose loading in maize and yield factors. This study discussed the mechanism of exogenous ABA alleviating maize ear dysplasia at grain filling stage under drought stress from the perspective of photosynthesis and sucrose transport.

Cytogenetic impact of gamma radiation and its effects on growth, yield and drought tolerance of maize (Zea mays L.).

Maize is the third most important grain crop worldwide after wheat and rice; it is a vital global crop, serving as a key source of food, animal feed, and industrial products, making it essential for food security and economic stability in many countries. Drought stress adversely affects water uptake and can stunt growth, reducing the overall productivity of maize. So, this study was carried out to investigate the cytogenetic effects of gamma radiation and drought stress on maize SC131 genotype, focusing on chromosomal aberrations in seedling root meristems induced by varying doses of gamma irradiation (50, 100, 150, 200, and 250 Gray) and drought stress imposed by 10% polyethylene glycol (PEG). The present study also aims to evaluate the impact of these treatments on growth parameters under a controlled pot experiment. Additionally, molecular polymorphism induced by both gamma irradiation and drought stress was analyzed using Real-Time quantitative PCR techniques for DREB2, ERF, and EF transcription factors. Also, under a field condition experiment, maize plants were subjected to the same gamma irradiation doses and drought stress by reducing the number of irrigations, with subsequent evaluations of yield attributes to assess the overall impact of treatments on plant performance. The study also investigates the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) banding patterns of proteins in grains yielded under the influence of gamma radiation and drought treatments. Findings of the current investigation indicate that the low dose of gamma radiation (50 Gray) not only induces cytogenetic changes but also enhances drought tolerance and improves yield characteristics, suggesting that targeted gamma irradiation could serve as a viable strategy to bolster maize resilience in challenging environmental conditions.

In-vitro effectiveness of aqueous extract of organic compost against Helminthosporium turcicum (cryptogamic disease pathogen of maize)

Sustainable agriculture requires the application of biofertilizers and biopesticides in farming systems. The aim of the study was to analyze in-vitro the effect of aqueous extracts from organic compost on the development of cryptogamic disease in maize plants for yield improvement. The study was carried out in the laboratory using a pure strain of Helminthosporium turcicum previously isolated from infected maize leaves showing symptoms of the disease. Aqueous substrates of unsterilized and sterilized composts, with the concentrations 0.5, 1.0 and 2.0 mL were respectively mixed with liquid PDA at 19.5; 19 and 18 mL for a final volume of 20 ml, then aseptically poured into 90 mm diameter Petri dishes under the laminar flow hood. After solidification, 7 mm-diameter mycelial discs from young H. turcicum cultures were deposited on the agar pellets. 48 hours after incubation, mycelial growth was measured 4 times until the positive control Petri dish was invaded by mycelial filaments. The results showed that the mycelial growth of the H. turcicum strain in the presence of the sterilized aqueous compost extract was significantly reduced. Increasing the concentration significantly inhibited strain growth to the order of 95.89% at a concentration of 2 mL sterilized aqueous compost extract. Similarly, in the presence of the unsterilized aqueous compost extract, the mycelial growth of H. turcicum was also reduced. The Increasing concentration inhibited the pathogen growth by up to 76.92%, at the concentration of 2 mL. These results showed that the aqueous extract of organic compost contains compounds that could be responsible for producing suppressive substances accountable for the mechanisms inhibiting the mycelial growth of H. turcicum. Int. J. Agril. Res. Innov. Tech. 14(2): 62-73, December 2024

New insights into the responses of phosphite, as a plant biostimulator, on PSII photochemistry, gas exchange, redox state and antioxidant system in maize plants under boron toxicity

New insights into the responses of phosphite, as a plant biostimulator, on PSII photochemistry, gas exchange, redox state and antioxidant system in maize plants under boron toxicity

Agronomic Practices Adopted by the Maize (Zea mays) Farmers in Horowpathana DS Division in Anuradhapura, Sri Lanka

Maize (Zea mays L.) is the third most important cereal crop globally and a vital crop in Sri Lanka, particularly in the Dry and Intermediate zones. This survey was conducted to assess the major challenges faced by maize farmers and the agronomic practices they adopt in the Horowpathana area of the Anuradhapura District, Sri Lanka. A total of 60 farmers from 12 Grama Niladhari (GN) divisions were randomly selected for the study. Data were collected through a questionnaire survey, and descriptive and inferential statistical analyses were performed. The findings revealed that the majority of maize farmers were aged between 36 and 40 years, with both men and women engaged in cultivation. The majority of farmers were educated, yet a significant proportion did not use organic fertilizers, relying primarily on chemical fertilizers. Herbicide use was common, with all farmers applying it to control weeds after planting maize. The study identified several challenges faced by farmers, including adverse weather conditions such as drying and high winds, which were reported by 18.3% of the farmers. Strong winds were found to cause significant damage to maize plants, particularly during the rainy season. Additionally, farmers experienced financial constraints, spending significant amounts on land preparation, chemicals, seeds, labor, transportation, and harvesting, leading to a low-income economy. Price fluctuations of maize grain, lack of storage facilities, and high transportation costs were also major issues affecting the marketing of maize. Biotic and abiotic constraints, such as weeds, drought, low soil fertility, and high input costs, were identified as key factors limiting production. The study concluded that most farmers did not adhere to recommended management practices, resulting in severe yield losses. Therefore, it is crucial to implement awareness programs to encourage proper management practices, ensuring the sustainability of maize production and safeguarding the local economy.

Seasonal and Spatial Distribution of Fall Armyworm Larvae in Maize Fields: Implications for Integrated Pest Management

The fall armyworm (FAW), Spodoptera frugiperda, a major pest in maize production, was assessed for its temporal and spatial distribution in maize fields during both the dry and rainy seasons of 2021 and 2022 in two agroecological regions in Benin (zone 6 and 8). Zone 6 (AEZ 6) “called zone of terre de barre” (Southern and Central Benin) consisted of ferralitic soils, a Sudano-Guinean climate (two rainy seasons alternating with two dry seasons) with a rainfall ranging between 800 and 1400 mm of rainfall per year; while zone 8 (AEZ 8) called “fisheries region” (Southern Benin” is characterized by coastal gleysols and arenosols with a Sudano-Guinean climate and a rainfall of 900–1400 mm of rainfall per year. In this study, 30 and 50 maize plants were randomly sampled using a “W” pattern during the dry and rainy seasons, respectively. Larval density, larval infestation rates, and damage severity were monitored over time. Taylor’s power law and the mean crowding aggregation index were applied to evaluate the dispersion patterns of the larvae. The results indicate a higher larval infestation rate and larval density in AEZ 8 compared to AEZ 6 during the dry season. In the rainy season, while the percentage of damaged plants was higher in AZE 8, no significant differences in larval density between the two zones were observed. The dispersion analysis revealed moderate aggregation (aggregation index = 1.25) with a basic colony of 2.08 larvae, i.e., an average initial cluster of 2.08 larvae observed per plant, reflecting the aggregation oviposition behavior of FAW. This study provides valuable monitoring data on the FAW’s distribution, offering insights for further research on population dynamics and developing predictive models for integrated pest management strategies.

Maize plant development and antioxidant defense influenced by selenium-enriched Cyanobacteria species

Maize plant development and antioxidant defense influenced by selenium-enriched Cyanobacteria species

Effect of Zinc Sulfate and Adjuvants on the Physicochemical Properties and Efficacy of Bromoxynil with Terbythylazine and Nicosulfuron Mixtures Against Echinochloa crus-galli L.

The study aimed to evaluate the effects of methyl esters adjuvant, pH reducer, and zinc sulfate on the physicochemical properties (contact angle, surface tension) of a spray liquid and a bromoxynil + terbuthylazine + nicosulfuron mixture’s efficacy. Cockspur (Echinochloa crus-galli L.) was used as the test plant. The placement of any adjuvant in the spray liquid affected the reduction in contact angle, with zinc sulfate reducing it from 75.9 to 66.3°, methyl esters adjuvant from 61.8 to 47.1°, pH reducer from 58.3 to 47.0°, zinc sulfate + methyl esters adjuvant from 64.9 to 58.4°, and zinc sulfate + pH reducer from 57.1 to 44.6°. A decrease in contact angle was found with a reduction in herbicide doses, from 65.6 to 59.0°. The highest pH of the spray liquid with herbicides was found when the methyl esters adjuvant was added to the liquid (6.82–7.17), followed by 6.43–6.80 when zinc sulfate was added, and 6.05–6.30 for zinc sulfate with methyl esters adjuvant. The inclusion of adjuvant pH reducer very strongly reduced the liquid reaction to 3.28–3.60, and it was reduced to 2.76–2.90 in the presence of zinc sulfate. Bromoxynil + terbuthylazine + nicosulfuron mixtures applied with methyl esters adjuvant and zinc sulfate with pH reducer showed the highest efficacy (85–98% and 82–96%), and the efficacy was 64–81% when methyl esters adjuvant with zinc sulfate were used. The effect of the herbicide mixture with only zinc sulfate was noticeably weaker (40–81%), and it was very weak (13–43%) in the presence of adjuvant pH reducer only. On the basis of the ED50 values, the most favorable mixtures were those containing methyl esters adjuvant, methyl esters adjuvant + zinc sulfate and pH reducer + zinc sulfate in addition to the herbicides. Research has indicated that zinc sulfate could be used as an adjuvant, and will support not only the action of herbicides, but also the development of maize plants.

Gut bacteria of Spodoptera frugiperda establish endophytic association and affect the interactions of their host herbivore with maize plants

Gut bacteria of Spodoptera frugiperda establish endophytic association and affect the interactions of their host herbivore with maize plants

Maize transcriptome profiling reveals low temperatures affect photosynthesis during the emergence stage

IntroductionEarlier sowing is a promising strategy of ensuring sufficiently high maize yields in the face of negative environmental factors caused by climate change. However, it leads to the low temperature exposure of maize plants during emergence, warranting a better understanding of their response and acclimation to suboptimal temperatures.Materials and MethodsTo achieve this goal, whole transcriptome sequencing was performed on two maize inbred lines – tolerant/susceptible to low temperatures, at the 5-day-old seedling stage. Sampling was performed after 6h and 24h of treatment (10/8°C). The data was filtered, mapped, and the identified mRNAs, lncRNAs, and circRNAs were quantified. Expression patterns of the RNAs, as well as the interactions between them, were analyzed to reveal the ones important for low-temperature response.Results and DiscussionGenes involved in different steps of photosynthesis were downregulated in both genotypes: psa, psb, lhc, and cab genes important for photosystem I and II functioning, as well as rca, prk, rbcx1 genes necessary for the Calvin cycle. The difference in low-temperature tolerance between genotypes appeared to arise from their ability to mitigate damage caused by photoinhibition: ctpa2, grx, elip, UF3GT genes showed higher expression in the tolerant genotype. Certain identified lncRNAs also targeted these genes, creating an interaction network induced by the treatment (XLOC_016169-rca; XLOC_002167-XLOC_006091-elip2). These findings shed light on the potential mechanisms of low-temperature acclimation during emergence and lay the groundwork for subsequent analyses across diverse maize genotypes and developmental stages. As such, it offers valuable guidance for future research directions in the molecular breeding of low-temperature tolerant maize.

Design and testing of a posture-adjusting precision metering device for high-speed maize planting

IntroductionThis study introduces a new technical method that involves adjusting the seed-filling posture and fluidizing the seed group to improve the capacity of mechanical seed-metering devices for high-speed maize planting. The design of a novel posture-adjusting seed-metering device is presented and its operating principle is described. In the seed-filling area, the seeds maintain the same postural characteristics and are detached from the seed group. This method creates favorable conditions for mechanical seed-metering devices to achieve effective seed-filling under high-speed planting conditions.MethodsFirstly, the key parameters of the core component were analyzed. Secondly, the influences of the seed posture adjusting efficiency and seed flow distribution characteristics on the seed-filling effect were clarified by the EDEM simulation. Finally, the high-speed metering performance was optimized and validated using bench testing.ResultsThe experiment demonstrated that the type A groove had the highest seed posture adjusting efficiency, a more uniform seed flow distribution, and a superior seed-filling effect as compared to the other groove types. The best metering performance was achieved with an opening angle of 21.6° and an opening width of 8.4 mm; this resulted in pass, repeat, and leak rates of 94.8%, 1.3%, and 3.9%, respectively. Within the speed range of 8–14 km/h, the posture-adjusting seed-metering device demonstrated a pass rate of over 94%, a repeat rate below 2.1%, and a leak rate below 3.9%.DiscussionThe designed device had a better high-speed planting capacity than a seed-metering device without a posture-adjusting mechanism, thus proving the effectiveness of the novel seed-filling method. These findings provide a reference for improving the high-speed planting capacity of mechanical seed-metering devices.

Evaluation of the Control Efficacy of Bt Maize Expressing Cry1Ab and Vip3Aa Proteins Against Agrotis ypsilon (Rottemberg)

Bt maize is the main means to control many lepidopteran pests in the world, but its control efficacy against Agrotis ypsilon (Rottemberg), an important insect pest of maize seedlings, remains unclear until now. The interaction between the insect and Bt transgenic maize events (DBN9936 (expressing Cry1Ab), DBN9501 (expressing Vip3Aa), and DBN3601T (expressing Cry1Ab and Vip3Aa)) was investigated using bioassay and insect behavioral tests. The results show that the Cry1Ab contents in different tissues of DBN9936 were 47.78–82.60 μg·g−1, and the Vip3Aa contents in DBN9501 were 15.29–27.78 μg·g−1. The contents of Cry1Ab and Vip3Aa in DBN3601T were 32.08–79.08 and 10.16–17.52 μg·g−1, respectively. There was no significant difference in total Bt protein content between the leaves and stems; however, that the content in both was significantly higher than that in the roots. The larvae were most sensitive to the Vip3Aa protein, and the corrected mortalities of larvae feeding on DBN9501 and DBN3601T were greater than 89.65% at the seedling stage, significantly higher than those feeding on DBN9936 (16.46–76.13%). The corrected mortalities of the third to the fifth instar larvae feeding on Bt maize root were as follows: DBN3601T (54.00–96.60%) > DBN9501 (24.67–70.88%) > DBN9936 (6.67–53.31%). The results of behavioral tests for Bt/non-Bt maize plant selection indicated that the larvae mainly fed on non-Bt maize while showing antifeedant behavior toward Bt maize, and the moth preferred to lay eggs on undamaged or slightly damaged Bt maize. It is concluded that DBN3601T maize has a strong control efficacy for A. ypsilon, which can play an important role in building an integrated pest management strategy for the insect.

Unveiling the potential of Loigolactobacillus coryniformis BCH-4 to ameliorate drought stress in Zea mays L.

The lactic acid bacterial (LAB) species have proven multifaceted roles in sustainable agriculture due to their biologically safe nature, making them eco-friendly. However, their plant growth-improving mechanisms in stressed and non-stressed conditions are still under consideration. Thus, the current work has been planned to evaluate the drought tolerance potential and plant growth-promoting (PGP) traits of Loigolactobacillus coryniformis BCH-4 in Zea mays L. This bacterium was tolerant in the presence of 30% PEG. It also exhibited auxin synthesis, ammonia production, and phosphate solubilization potential. Based on these findings, a pot experiment was conducted with selected drought-tolerant maize seeds (Sawari Kashmir) and drought-sensitive seeds (Malka-2016), bioprimed with L. coryniformis under 2 water regimes; 100% field capacity (optimal watering condition) and 50% field capacity (a drought stress condition). Our results manifested an upsurge in maize growth, photosynthetic pigments, and nutrient uptake in bioprimed seeds under stressed conditions. Besides, biopriming considerably improved nitrate reductase levels alongside higher nitric oxide and hydrogen sulphide activity. This treatment also improved drought-induced oxidative stress tolerance in maize plants by promoting osmolytes and antioxidant activities. Further, the GC-MS analysis revealed various drought-tolerant metabolites, known to have plant growth-promoting potential i.e., 1-hexadecene, tetradecane, hexadecanoic acid, 15-methyl-, methyl ester, and hexadecane, produced by L. coryniformis. Remarkably, these metabolites synergistically improve maize growth and tolerate the drought. This study publicized an extensive variety of metabolites, produced by L. coryniformis liable for plant growth promotion and drought tolerance.

Microbiome-Mediated Mechanisms Regulating Adaptability to Iron Deficiency in the Intercropping System of Soybean and Maize

Iron (Fe) deficiency is a pervasive agricultural concern on a global scale. Intercropping plays a pivotal role in activating soil nutrient cycling and crop nutrient uptake and utilization. This study integrates plant physiology, soil physicochemical determination, high-throughput sequencing, and metabolomics techniques to conduct pot experiments using field-collected soils with soybean and maize plants. This study aims to investigate the mechanisms through which microorganisms in a soybean–maize intercropping system regulate Fe deficiency adaptation. The results revealed that intercropping enhances the resilience of soybean and maize in Fe-deficient environments, facilitates nutrient absorption by plants, and enriches soil nutrient content. Moreover, intercropping fostered more intricate microbial interactions in comparison to monocropping. The dominant microorganisms in the rhizosphere of intercropped soybean and maize included genera Microbacterium, Sphingomonas, Shinella, and Rhizobium. Microbacterium, Sphingomonas, Shinella, and Rhizobium have the potential to produce Fe chelators or enhance plant Fe absorption. Additionally, intercropping notably modified the composition of root exudates derived from soybean and maize. The soybean and maize rhizosphere exhibited significant enrichment with oleamide, coumestrol, glycitein, and daidzein. Coumestrol may have an effect of promoting Fe absorption, and it is significantly positively correlated with the genus Nakamurella in the maize rhizosphere and the genus Pirellula in the soybean rhizosphere. Consequently, these findings suggested that the rhizosphere of intercropped soybean and maize significantly enriches specific microbial communities and root exudates, thereby enhancing microecosystem stability and improving plant tolerance to Fe deficiency.

Plant Height Estimation in Corn Fields Based on Column Space Segmentation Algorithm

Plant genomics have progressed significantly due to advances in information technology, but phenotypic measurement technology has not kept pace, hindering plant breeding. As maize is one of China’s three main grain crops, accurately measuring plant height is crucial for assessing crop growth and productivity. This study addresses the challenges of plant segmentation and inaccurate plant height extraction in maize populations under field conditions. A three-dimensional dense point cloud was reconstructed using the structure from motion–multi-view stereo (SFM-MVS) method, based on multi-view image sequences captured by an unmanned aerial vehicle (UAV). To improve plant segmentation, we propose a column space approximate segmentation algorithm, which combines the column space method with the enclosing box technique. The proposed method achieved a segmentation accuracy exceeding 90% in dense canopy conditions, significantly outperforming traditional algorithms, such as region growing (80%) and Euclidean clustering (75%). Furthermore, the extracted plant heights demonstrated a high correlation with manual measurements, with R2 values ranging from 0.8884 to 0.9989 and RMSE values as low as 0.0148 m. However, the scalability of the method for larger agricultural operations may face challenges due to computational demands when processing large-scale datasets and potential performance variability under different environmental conditions. Addressing these issues through algorithm optimization, parallel processing, and the integration of additional data sources such as multispectral or LiDAR data could enhance its scalability and robustness. The results demonstrate that the method can accurately reflect the heights of maize plants, providing a reliable solution for large-scale, field-based maize phenotyping. The method has potential applications in high-throughput monitoring of crop phenotypes and precision agriculture.

ZmHB53, a Maize Homeodomain-Leucine Zipper I Transcription Factor Family Gene, Contributes to Abscisic Acid Sensitivity and Confers Seedling Drought Tolerance by Promoting the Activity of ZmPYL4.

Plant-specific homeodomain-leucine zipper I (HD-Zip I) transcription factors (TFs) crucially regulate plant drought tolerance. However, their specific roles in maize (Zea mays L.) regulating drought tolerance remain largely unreported. Here, we screened a maize HD-Zip I TF family gene, ZmHB53, and clarified its role in drought stress. ZmHB53 overexpression maize plants exhibited sensitivity to abscisic acid (ABA), tolerant to polyethylene glycol (PEG 6000)-induced stress during germination, along with improved seedling drought resistance. Compared to the wild-type, ZmHB53 overexpression lines show higher water retention, biomass, and survival rates, and reduced water loss and stomatal size under drought, suggesting ZmHB53's role in drought adaptation. DNA affinity purification sequencing (DAP-Seq), yeast one hybrid, electrophoretic mobility shift assay (EMSA), and dual luciferase showed that ZmHB53 directly bound to and upregulated the expression of ABA receptor ZmPYL4. Meanwhile, transgenic plants overexpressing ZmPYL4 also exhibit ABA sensitivity and drought tolerance. The research results provide novel insights into the regulatory role of ZmHB53 and ZmPYL4 in enhancing maize's drought tolerance, establishing a foundation for future validation and potential application of ZmHB53 in strategies to improve maize resistance to drought.

Impact of dumpsite compost on heavy metal accumulation in some cultivated plants

The study examines the concentrations of heavy metals in agricultural soil, compost from landfills, maize plants, and spinach crops. The results show that compost from landfills had levels exceeding EU requirements for Cd, Cu, Ni, Pb, and Zn. However, agricultural soil contained trace amounts of heavy metals. The order of metal absorption by plants was Cd > Pb > Cu > Ni > Cr > Zn > Fe > Mn. This highlights potential health risks associated with consuming crops grown in compost or soil tainted with heavy metals.

Construing the resilience to osmotic stress using endophytic fungus in maize (Zea mays L.).

In a wake of shifting climatic scenarios, plants are frequently forced to undergo a spectrum of abiotic and biotic stresses at various stages of growth, many of which have a detrimental effect on production and survival. Naturally, microbial consortia partner up to boost plant growth and constitute a diversified ecosystem against abiotic stresses. Despite this, little is known pertaining to the interplay between endophytic microbes which release phytohormones and stimulate plant development in stressed environments. In a lab study, we demonstrated that an endophyte isolated from the Kargil region of India, a Fusarium equiseti strain K23-FE, colonizes the maize hybrid MAH 14 - 5, promoting its growth and conferring polyethylene glycol (PEG)-induced osmotic stress tolerance. To unravel the molecular mechanism, maize seedlings inoculated with endophyte were subjected to comparative transcriptomic analysis. In response to osmotic stress, genes associated with metabolic, photosynthesis, secondary metabolites, and terpene biosynthesis pathways were highly upregulated in endophyte enriched maize seedlings. Further, in a greenhouse experiment, maize plants inoculated with fungal endophyte showed higher relative leaf water content, chlorophyll content, and antioxidant enzyme activity such as polyphenol oxidase (PPO) and catalase (CAT) under 50% field capacity conditions. Osmoprotectant like proline were higher and malondialdehyde content was reduced in colonized plants. This study set as proof of concept to demonstrate that endophytes adapted to adverse environments can efficiently tweak non-host plant responses to abiotic stresses such as water deficit stress via physiological and molecular pathways, offering a huge opportunity for their deployment in sustainable agriculture.

Enhancement of Maize (Zea mays var: GS-2) Plant Growth and Yield: Seed Treatment with Non-thermal Plasma using Different Gases

Impact of pre-sowing treatment of maize (Zea mays Var: GS-2) seeds with non-thermal glow discharge plasma on the growth and yield of field grown plants has been investigated. Maize seeds were treated with non-thermal glow discharge plasma produced from five different gases Nitrogen (N2), Helium (He), Argon (Ar), Hydrogen (H2) and Oxygen (O2) before sowing the seeds in the field. Seeds were exposed to 30 W for 240 seconds to glow discharge plasma produced in a cylindrical stainless steel vacuum chamber utilizing an automatic matching network and a 13.56 MHz with the capacity up to 600W RF power source are used. Plasma treated seeds were grown in the field and growth measurements were made at 30, 60 and 90 days after sowing. Pre- sowing treatment with glow discharge plasma enhanced plant height, leaf length and plant fresh weight at all the stages of growth. O2 and N2 plasma treated seeds were more effective than He, H2 and Ar in enhancing the growth and biomass of the plants. The yield parameters are cob length 21% in O2 and 19% in N2, cob weight 36% in O2 and 34% in N2, and 100 seeds weight 39% in O2 and 36% in N2 plasma were significantly enhanced. It is concluded that O2 and N2 plasma Pre-sowing treatment can significantly enhance the biomass and yield of maize plants with O2 slightly higher. Analyzing the characterization with scanning electron microscopy (SEM), and optical emission spectroscopy (OES) of maize seeds. SEM revealed considerable alterations for O2, N2, Ar, and He but no change for H2 plasma treatment. The findings demonstrate that the surface of the starch caryopsis has been altered, exposing an oxygen and nitrogen plasma that has resulted in huge channels and water holes. The OES identified the presence of OH, NO, O, and N2 radicals generated during oxygen and nitrogen plasma treatment, which is contributing to the best result.

A holistic approach for the evaluation of iron nanoparticles on maize plants and earthworms in natural soil.

There is a debate about the implications of the effect of nanoparticles or nanomaterials on edible plants and soil organisms. Earthworms have been used to evaluate soil quality, reproduction, survival, and other biochemical parameters when organisms are exposed to nanomaterials. Most studies have been performed in laboratory settings, and little has been studied under realistic conditions, especially when earthworms and corn plants share the same natural soil and organic matter space. The objective of this research was to evaluate concentrations of 10, 100, 200, 400, and 800mgkg-1 of iron nanoparticles (Fe NPs, combination of magnetite [Fe3O4] and hematite [α-Fe2O3]) on the growth and development of maize plants (Zea mays L.), and of the reproduction parameters of Eisenia fetida during 110 days of exposure. The results demonstrated that concentrations of 100 and 400mgkg-1 of Fe NPs positively changed some morphological, photosynthetic, and yield parameters in maize crops. Unexpectedly, concentrations of 400 and 800mgkg-1 decreased earthworm population but, at the same time, increased the number of juveniles. With this holistic approach experiment, it was demonstrated that the use of Fe nanoparticles as a biostimulant can promote corn yield parameters, but at the same time it impacts the reproductive capacity of the earthworms. However, it is necessary to explore the long-term effect of the proposed nanomaterials in agriculture to rule out risks or ecological implications in agricultural systems.