Plant Yield Research Articles

Biochar Enhances Rice Growth and Mitigates Total Petroleum Hydrocarbon Contamination in Soil: A Sustainable Approach for Crop Production

ABSTRACT Biochar is a soil amendment made from organic waste pyrolysis that has the potential to enhance crop growth and reduce soil contamination. A recent study examined the effects of biochar application on rice plant growth, yield, biomass, and total petroleum hydrocarbon (TPH) contamination in soil. The study included four treatments: control, biochar (2.0% and 4.0% addition), TPH (4.0%, 6.0%, and 8.0%), and biochar+TPH. Results showed that biochar application was effective in mitigating the negative effects of TPH contamination on soil-physiochemical properties, increasing soil pH, organic matter, and available nutrients. Additionally, biochar application significantly increased rice plant height compared to the control and mitigated the negative impact of TPH on rice plant height in TPH-contaminated soil. Biochar application at 4.0% and 6.0% resulted in higher grain yield and biomass than the control group under all TPH contamination levels, with 6.0% being the most effective. Overall, the study highlights the potential of biochar as a sustainable approach for crop production in contaminated areas. However, further research is required to understand the underlying mechanisms, sustainability, and long-term effects of biochar application in agricultural systems.

Growth and Flowering of Lilium (Lilium longiflorum) cv. Eremo as Affected by CCC and GA3 under Naturally Ventilated Polyhouse Conditions of Prayagraj

The present investigation was carried out under naturally ventilated polyhouse conditions, Department of Horticulture, SHUATS, Prayagraj, during November, 2023 to March, 2024. The experiment was laid out in Randomized Block Design (RBD) with three replications. There were nine treatments comprising of Cycocel (CCC) and Gibberellic Acid (GA3) at different concentrations viz., GA3 (100, 200, 300 and 400 ppm) and CCC (100, 200, 300 and 400 ppm) along with Water spray. The results revealed foliar application of 300 ppm GA3 for Lilium significantly enhanced the number of leaves (71.83), plant height (76.47 cm), plant spread (15.44 cm), leaf area (9.73 cm2),number of buds (3.40),at 300 ppm of GA3 it takes lesser days taken for bud initiation(40.40),and also less days taken for first flower opening from planting (68.53), flower bud length (8.83 cm), flower stalk length (12.43 cm), stalk length (78.10 cm), self-life  (12.97 days),flower yield per plant (3.40) and flower yield for 250 m2(6296.29) . Among the different treatments, the highest Gross return is 188888.7 Rs/250 m2, Net return is 120870.75 Rs/250 m2 and Benefit cost ratio is 2.77, obtained by the application of 300 ppm of GA3.Whereas in terms of only Bulb parameters, foliar application of 200 ppm of CCC for Lilium reported maximum number of bulblets per plant (4.11) and gives maximum weight of bulb per plant (74.07g).

Effect of eggshell powder and phosphorus on growth and yield of MD7 groundnut variety in spring season in Phu Xuyen, Ha Noi

The experiment was conducted to evaluate the effect of eggshell powder and phosphorus on growth and yield of MD7 groundnut variety in the spring season in Phu Xuyen, Ha Noi. The two factor experiment was laid out in a split-plot design with three replications. The main plot consisted of three rates of eggshell powder (0, 300, 500 kg/ha). The sub-plot included three rates of phosphorus (30, 60, 90 kg/ha). The results showed that the growth and yield of plants fertilized with eggshell powder were higher than those with unfertilized eggshell powder. In addition, the growth traits and yield of MD7 groundnut variety increased with increasing phosphorus fertilizer doses from 30 kg P2O5/ha to 90 kg P 2O5/ha under the same fertilized or unfertilized eggshell powder conditions. Briefly, the results indicated that the rate of 500 kg eggshell powder/ha combined with 90 kg P2O5/ha was suitable for MD7 groundnut variety to grow, develop and produce the highest yield in the spring season in Phu Xuyen, Ha Noi.

Growth and Yield of Summer Squash (Cucurbita pepo L.) as Influenced by Different Coloured Plastic Mulches in the Tarai Region of Uttarakhand

Summer squash is a short-duration vegetable crop with off-season nature and high economic returns per unit area. However, the early crop growth season encounters a number of abiotic stresses like low temperature and frost, which adversely affect the marketable yield and quality of the fruits. This dependency on natural factors restricts the scope of its profitable cultivation, thereby making it necessary to bring more area under protected cultivation. Coloured plastic mulches are an important component of protected cultivation, which are designed to modify the microclimate at the plant and soil levels so as to increase crop production and productivity, as well as quality of the produce, to meet the demands of the consumers. Keeping these considerations in view, the present field investigation was conducted at the Vegetable Research Centre of G. B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand) during the rabi season of 2019-20 to study the effect of different coloured plastic mulches on the growth and yield of summer squash (Cucurbita pepo L.). The experiment comprised nine treatments (bi-coloured plastic mulches) replicated thrice in a randomised block design. Results revealed that use of different coloured plastic films significantly influenced the growth and yield parameters of summer squash. Red coloured plastic mulch (V1) recorded the maximum values for fruit length (30.78 cm), fruit yield plant-1 (1975.8 g), fruit yield plot-1 (39.51 kg) and total marketable fruit yield (365.52 q ha-1), while biodegradable film of silver-black colour (V7) and black plastic mulch (V4) recorded the maximum plant height (cm) and plant spread (cm), respectively. The maximum diameter of the fruit (110.13 mm) was recorded under black coloured plastic mulch. Considering the prevalent climatic conditions of the tarai region of Uttarakhand, red coloured plastic mulch can be recommended for commercial cultivation of summer squash for obtaining higher fruit yields.

Effect of Selenium and Garlic Extract Treatments of Seed-Addressed Lettuce Plants on Biofortification Level, Seed Productivity and Mature Plant Yield and Quality.

The enhancement of the plant seed yield and quality represents the basis of the successful productivity of the deriving crop. The effect of single and combined foliar treatments of lettuce plants with sodium selenate and garlic bulb extract on seed yield and quality and on mature plant biochemical characteristics was investigated using four lettuce cultivars (Bouquet, Picnic, Moskovsky parnikovy and Cavalier). The seed production of plants treated with Se increased by 20-41%, compared to the untreated control plants, while the augmentation was as much as 10-23% and 17-27% under garlic extract and the joint application of Se and garlic, respectively. Garlic extract stimulated the accumulation of Se in lettuce seeds, which rose by 1.21-1.29 times compared to the Se-treated plants. The proline levels in lettuce seeds exceeded the corresponding values recorded in the control ones by 1.32-1.64 times in the case of the Se supply, 1.10-1.47 times upon garlic extract application and 1.09-1.31 times under the combined Se/garlic treatment. All the treatments given to lettuce plants increased the leaf weight by 1.10-1.30 times, compared to the untreated control. The seed Se levels positively correlated with the leaf weight (r = 0.621; p < 0.005), chlorophyll (r = 0.672, p < 0.002) and total antioxidant activity (AOA; r = 0.730, p < 0.001) of plants grown from these seeds. Positive correlations were also recorded between the seed proline content and lettuce plant leaf weight, chlorophyll and AOA (r = 0.868, 0.811 and 0.815, respectively, at p < 0.001). Lettuce yield was positively correlated with the leaf AOA, chlorophyll and ascorbic acid content (r = 0.942, 0.921 and 0.665, respectively, at p < 0.001). The results indicate high prospects of Na2SeO4 and garlic extract application to seed-addressed lettuce plants, to improve seed productivity and quality, as well as lettuce yield and quality.

Investigating foliar application of bulk and nanoparticles titanium dioxide on fennel productivity to mitigate the negative effects of saline irrigation water

BackgroundFennel essential oils are fragrance compounds used in food and pharmaceutical sectors. One of the major impediments to expansion of fennel farming in Egypt's reclamation areas is saline water. Titanium dioxide (TiO2) or TiO2 nano particles (TiO2NP) can be utilized to boost the yield of aromatic plants cultivated under saline irrigation water. Saline water, particularly which contains sodium chloride can harm fennel plant; consequently, it was predicted that fennel production would fail in Egypt's reclaimed area, where the primary source of irrigation is groundwater consisting sodium chloride. This study sought to help fennel respond to sodium chloride by applying Ti forms to their leaves in order to reduce the detrimental effects of sodium chloride on them for expanding their production in the newly reclamation areas as a natural source of essential oil. Ti forms were applied as foliar application at 0, 0.1, 0.2 TiO2, 0.1 TiO2NP, and 0.2 TiO2NP, mM under irrigation with fresh water (0.4 dS m−1), or saline water (51.3 mM or 4.7 dS m−1).ResultsPlants exposed to 0.1 mM TiO2NP under fresh water resulted in the maximum values of morphological characters, estragole, oxygenated monoterpenes and photosynthetic pigments; while those subjected to 0.1 mM TiO2NP under saline water gave the greatest values of essential oil, proline, antioxidant enzymes and phenols. The greatest amounts of soluble sugars were recorded with 0.2 mM TiO2NP irrigated with saline water. Plants subjected to 0 mM TiO2 under saline water produced the greatest values of flavonoids, hydrogen peroxide and malondialdehyde.ConclusionTo mitigate the negative effects of salty irrigation water on fennel plant production, TiO2NP application is suggested as a potential strategy.

Grain yield, water-land productivity and economic profit responses to row configuration in maize-soybean strip intercropping systems under drip fertigation in arid northwest China

Intercropping has great potential for alleviating arable land competition, improving land output and promoting sustainable agricultural development. However, the applicability of maize-soybean strip intercropping under drip fertigation in arid northwest China remains unclear, especially under various row configurations. A two-season (2022 and 2023) field experiment was performed in the Hexi Region of northwest China to investigate the responses of plant growth, yield performance, water-land productivity and economic profit of drip-fertigated maize-soybean strip intercropping systems to eight row configurations. The results showed that intercropping significantly reduced aboveground biomass accumulation of maize and soybean by 18.77% and 47.81% on average compared to monocropping, respectively. Intercropping significantly decreased the 100-grain weight, ear length and ear width of maize, and reduced the 100-grain weight and pod number of soybean, resulting in reduced grain yields of intercropped maize and soybean (by 13.08% and 48.73%, respectively), but two rows of maize alternating with four rows of soybean (M2S4), three rows of maize alternating with four rows of soybean (M3S4), four rows of maize in wide and narrow rows alternating with four rows of soybean (M4S4-MN), and four rows of maize in wide and narrow rows alternating with six rows of soybean (M4S6-MN) produced greater population grain yield compared to monocropping. Among all intercropping systems, the largest water-land productivity and economic profit occurred in M2S4 (1.61 in 2022 and 1.42 in 2023 for land equivalent ratio; 29.23 kg ha−1 mm−1 in 2022 and 28.22 kg ha−1 mm−1 in 2023 for water productivity; 23,965 CNY ha−1 in 2022 and 23,059 CNY ha−1 in 2023 for economic profit), followed by M4S4-MN (1.53 in 2022 and 1.36 in 2023 for land equivalent ratio; 27.11 kg ha−1 mm−1 in 2022 and 26.58 kg ha−1 mm−1 in 2023 for water productivity; 22,327 CNY ha−1 in 2022 and 22,224 CNY ha−1 in 2023 for economic profit). The M2S4 is thus the optimal row configuration for drip-fertigated maize-soybean strip intercropping systems in terms of grain yield, economic profit and land productivity, while the M4S4-MN is recommended by further considering the efficiency of mechanized sowing and harvesting.

Influence of Naphthalene Acetic Acid (PGR) on the Growth, Yield and Susceptibility to Specific Chili Paper Disease in Different Chilies Cultivars (Capsicum annuum) in the Upland of Baluchistan

Capsicum annuum, commonly known as chili pepper, is a significant spice crop worldwide, and its yield and fruit quality are important factors affecting economic returns for growers. However, these can be impacted by various abiotic and biotic stresses, including environmental conditions, nutrient deficiencies, pests, and diseases. Utilizing growth regulators for plants, such NAA, has emerged as a viable strategy to enhance growth, improve yield, and mitigate stress effects in chili production. To ascertain the impact of the plant growth regulator (NAA) on the development and yield of chilies, a field experiment was carried out. Plant pathogens pose a significant threat to agricultural productivity worldwide. The main challenge in this scenario is that the plant pathogens are dynamic organisms that adapt to changing environmental conditions and agricultural methods. Examples of this kind of evolution include the appearance and spread of novel diseases or more aggressive pathogens that are resistant to fungicides. Using three replications and seven treatments, randomized complete block design (RCBD) was used to perform the experiment. Control (applying no naphthalene acetic acid), 25 ppm, 50 ppm, 75 ppm, 100 ppm, 125 ppm, and 150 ppm applications of naphthalene acetic acid. The result shows that chili crop treated with application of NAA @ 125 ppm exhibited significantly maximum resulted with 80.95 cm plant height, 15.13 quantity of branches plant-1, 84.24 days to flowering, 100.02 days to fruit set, 8.65 cm fruit length, 42.89 g fruit weight, 36.72 quantity of fruits plant-1 and 0.43 kg yield plant-1. In contrast, chili treated under control exhibited the lowest performance, 53.98 cm plant height, 10.49 quantity of branches plant-1, 82.46 days to flowering, 90.77 days to fruit set, 5.66 cm fruit length, 22.54 g fruit weight, 20.39 quantity of fruits plant-1 and 0.02 kg yield plant-1. Among the chili variety, Hot chilies yielded the best results, with 69.31 cm plant height, 13.15 quantity of branches plant-1, 83.52 days to flowering, 96.05 days to fruit set, 7.39 cm fruit length, 34.07 g fruit weight, 29.71 quantity of fruits plant-1 and 0.25 kg yield plant-1. On the other hand, the variety SKY Cross showed the lowest performance, with 65.41 cm plant height, 12.49 quantity of branches plant-1, 83.24 days to flowering, 94.73 days to fruit set, 6.96 cm fruit length, 31.17 g fruit weight, 27.36 quantity of fruits plant-1 and 0.19 kg yield plant-1. After going through the findings of the present research, it was concluded that the application of NAA at 125 ppm showed superior results in terms of plant height, quantity of branches, days to flowering and fruit set, fruit length, fruit weight, quantity of fruits per plant, and overall yield per plant compared to other concentrations of NAA and the control group. The research findings show that that NAA at 125 ppm can be an effective growth regulator for chili crops, leading to improved growth and increased yields.

Exogenous melatonin delays leaves senescence and enhances saline and alkaline stress tolerance in grape seedlings

ABSTRACT Saline and alkaline stress is one of the major abiotic stresses facing agricultural production, which severely inhibits the growth and yield of plant. The application of plant growth regulators can effectively prevent crop yield reduction caused by saline and alkaline stress. Exogenous melatonin (MT) can act as a signaling molecule involved in the regulation of a variety of physiological processes in plants, has been found to play a key role in enhancing the improvement of plant tolerance to abiotic stresses. However, the effects of exogenous MT on saline and alkaline tolerance of table grape seedlings and its mechanism have not been clarified. The aim of this study was to investigate the role of exogenous MT on morphological and physiological growth of table grape seedlings ( Vitis vinifera L.) under saline and alkaline stress. The results showed that saline and alkaline stress resulted in yellowing and wilting of grape leaves and a decrease in chlorophyll content, whereas the application of exogenous MT alleviated the degradation of chlorophyll in grape seedling leaves caused by saline and alkaline stress and promoted the accumulation of soluble sugars and proline content. In addition, exogenous MT increased the activity of antioxidant enzymes, which resulted in the scavenging of reactive oxygen species (ROS) generated by saline and alkaline stress. In conclusion, exogenous MT was involved in the tolerance of grape seedlings to saline and alkaline stress, and enhanced the saline and alkaline resistance of grape seedlings to promote the growth and development of the grape industry in saline and alkaline areas.

Effective biological control of southern blight of Dendrobium officinale by Streptomyces sp. MEPF0303

Southern blight is a common and devastating disease adversely affecting the yield and quality of cultivated Dendrobium officinale plants. The pathogens responsible for this disease are Sclerotium delphinii and S. rolfsii, although S. delphinii is more pathogenic than S. rolfsii. Plant endophytes are potential sources of biological control agents. In this study, 18 microbial strains were isolated from wild Dendrobium shixingense plants (e.g., Streptomyces, Bacillus, and Trichoderma), among which strain MEPF0303 significantly inhibited S. delphinii mycelial growth. Based on 16S rRNA gene sequence, this strain was identified as Streptomyces sp. In addition, the volatile organic compounds and cell-free supernatant of MEPF0303 had significant inhibitory effects on S. delphinii growth. Moreover, MEPF0303 effectively controlled southern blight of D. officinale. A total of 1,797 differentially expressed genes were identified by a comparative transcriptome analysis of S. delphinii mycelia treated with MEPF0303 and the untreated control mycelia. Strain MEPF0303 significantly altered the expression of genes related to cell membrane morphological development and integrity, pathogenicity, energy metabolism, and oxidative stress responses. Furthermore, the transcriptome sequencing results were validated by analyzing gene expression via a quantitative real-time polymerase chain reaction. The study results showed that MEPF0303 can inhibit the growth and pathogenicity of S. delphinii, with potential implications for the biological control of southern blight of D. officinale.

Preparation of Plant Growth Enhancing Bioformulation from Agricultural Wastes by Solid State Fermentation

Preparation of organic waste based bioformulation through solid state fermentation technology for application in the field offers an ecofriendly and viable alternative to sustainable food production. The present study deals with the potential of seven agrowastes as substrate carriers for the growth of indigenous strains of Pseudomonas fluorescens (Pf) and Bacillus subtilis (Bs) that stimulate plant growth. The cosubstrate (CS) with equal proportion of the seven agrowastes and both the bioinoculants (CSPfBs) proved as the best substrate carrier with highest population load of the bioinoculants at 45 days after storage (DAS). Thereafter the population declined steadily in all the bioformulations from 60 DAS to 90 DAS. However, CSPfBs gave the highest population load at 90 DAS. As individual substrate for both Pf and Bs, vermicompost performed best with a population load of 9.95 of Pf and 9.98 of Bs at 90 DAS. The bioformulations CSPfBs, VBs and VPf when applied in soil, gave the best yield in brinjal plants (Solanum melongena L. cv. Pusa Kranti) at 30 days after transplanting. Similar trends were also observed in the other yield parameters. The study indicates the scope of the prepared bioinoculant as a low cost eco friendly option for promotion of growth and yield of crops.

Influence of the Nitrogen Fertilization on the Yield, Biometric Characteristics and Chemical Composition of Stevia rebaudiana Bertoni Grown in Poland.

Stevia rebaudiana Bertoni is a plant native to South America that has gathered much interest in recent decades thanks to diterpene glycosides, called steviosides, which it produces. These compounds are characterised by their sweetness, which is 250-300 times higher than saccharose, and they contain almost no caloric value. Stevia is currently also grown outside the South American continent, in various countries characterised by warm weather. This research aimed to determine whether it is viable to grow Stevia rebaudiana plants in Poland, a country characterised by a cooler climate than the native regions for stevia plants. Additionally, the impact of adding various dosages and forms of nitrogen fertiliser was analysed. It was determined that Stevia rebaudiana grown in Poland is characterised by a rather low concentration of steviosides, although proper nitrogen fertilisation can improve various characteristics of the grown plants. The addition of 100 kg or 150 kg of nitrogen per hectare of the field in the form of urea or ammonium nitrate increased the yield of the stevia plants. The stevioside content can be increased by applying fertilisation using 100 kg or 150 kg of nitrogen per hectare in the form of ammonium sulfate. The total yield of the stevia plants grown in Poland was lower than the yield typically recorded in warmer countries, and the low concentration of steviosides in the plant suggests that more research about growing Stevia rebaudiana in Poland would be needed to develop profitable methods of stevia cultivation.

Control of vapor pressure deficit (VPD) in micro-plant factory (McPF) to enhanced spinach microgreens growth

Harnessing the power of Vapor Pressure Deficit (VPD) control, this novel research illuminates a pathway to enhanced plant growth and yield within Micro-Plant Factory (McPF) and Urban Mini-Plant Factory (UMPF) environments. The research focused on the growth parameters of spinach microgreens, with the experimental data originating from each system's upper, central, and lower shelves. Results indicated significantly enhanced plant height and dry weight on the central shelves, attributed to optimal VPD control. Growth analysis also revealed higher relative growth rates (RGR) under tightly managed VPD conditions on the central shelves, even amidst varying environmental parameters. Most notably, effective VPD control translated these growth metrics into increased yields. For instance, a 71 % and 66 % increase in final plant dry weight over the lower and upper shelves was noted. These advances were mirrored in the plant height, which also saw 57 % and 103 % growth. Overall, the findings underscore the critical role of VPD control in plant growth and yield enhancement within structured environments like McPF and UMPF, offering significant insights for future research in manipulating VPD for optimal plant growth and productivity.

Co-applied magnesium nanoparticles and biochar modulate salinity stress via regulating yield, biochemical attribute, and fatty acid profile of Physalis alkekengi L.

While previous studies have addressed the desirable effects of biochar (BC) or magnesium nanoparticles (Mg NPs) on salinity stress individually, there is a research gap regarding their simultaneous application. Additionally, the specific mechanisms underlying the effects of BC and Mg NPs on salinity in Physalis alkekengi L. remain unclear. This study aimed to investigate the synergistic effects of BC and Mg NPs on P. alkekengi L. under salinity stress conditions. A pot experiment was conductedwith salinity at 100 and 200 mM sodium chloride (NaCl), as well as soil applied BC (4% v/v) and foliar applied Mg NPs (500 mg L-1) on physiological and biochemical properties of P. alkekengi L. The results represented that salinity, particularly 200 mM NaCl, significantly reduced plant yield (58%) and total chlorophyll (Chl, 36%), but increased superoxide dismutase (SOD, 82%) and catalase (CAT, 159%) activity relative to non-saline conditions. However, the co-application of BC and Mg NPs mitigated these negative effects and improved fruit yield, Chl, anthocyanin, and ascorbic acid. It also decreased the activity of antioxidant enzymes. Salinity also altered the fatty acid composition, increasing saturated fatty acids (SFAs) and polyunsaturated fatty acids (PUFAs), while decreasing monounsaturated fatty acids (MUFAs). The heat map analysis showed that fruit yield, anthocyanin, Chl, and CAT were sensitive to salinity. The findings can provide insights into the possibility of these amendments as sustainable strategies to mitigate salt stress and enhance plant productivity in affected areas.

PENGARUH WAKTU PENYIANGAN TERHADAP PERTUMBUHAN DAN HASIL TANAMAN JAGUNG HIBRIDA (Zea mays L)  VARIETAS N 37

This research was carried out at UPTB BP3K Sungai Lilin, Tanah Sepenggal Lintas District, Bungo Regency. This research was carried out from March 25 2014 to June 25 2014. The aim of this research was to determine the effect of weeding time and to determine the right weeding time for the growth and yield of corn plants. The design used in this research was a Randomized Group Design (RAK) with 5 treatments and 4 replications, the treatments were as follows: P0 (No weeding), P1 (weeding at 14 days after planting), P2 (weeding at 21 days after planting), P3 (weeding at 28 days after planting) and P4 (weeding at 35 days after planting). The variables observed were plant height (cm), total leaf area (cm2), age at start of flowering (DAT), net cob weight (g) and dry shelled corn yield (tons/ha). The results of the research show that different weeding times have a real influence on plant height (cm), total leaf area (cm2), age when flowering starts (DAP), net cob weight (g), but have no real effect on dry shelled corn yield ( tons/ha) in corn plants. Overall, weeding time at 14 days after planting has the best effect on the growth and yield of corn plants.

Biochar-nanoparticle combinations enhance the biogeochemical recovery of a post-mining soil

Here we addressed the capacity of distinct amendments to reduce arsenic (As), copper (Cu), selenium (Se) and zinc (Zn) associated risks and improve the biogeochemical functions of post-mining soil. To this, we examined nanoparticles (NPs) and/or biochar effects, combined with phytostabilization using Lolium perenne L. Soil samples were taken in a former metal mine surroundings. Ryegrass seeds were sown in pots containing different combinations of NPs (zero-valent iron (nZVI) or hydroxyapatite (nH)) (0 and 2 %), and biochar (0, 3 and 5 %). Plants were grown for 45 days and the plant yield and element accumulation were evaluated, also soil properties (element distribution within the soil fractions, fertility, and enzymatic activities associated with microbiota functionality and nutrient cycling) were determined. Results showed biochar-treated soil had a higher pH, and much higher organic carbon (C) content than control soil and NP-treated soils, and it revealed increased labile C, total N, and available P concentrations. Soil treatment with NP-biochar combinations increased exchangeable non-acid cation concentrations and reduced exchangeable Na%, improved soil fertility, reduced sodicity risk, and increased ryegrass biomass. Enzymatic activities, particularly dehydrogenase and glucosidase, increased upon the addition of biochar, and this effect was fostered by NPs. Most treatments led to a significant reduction of metal(loid)s contents in biomass, mitigating contamination risks. The two different NPs had similar effects in many parameters, nH outperformed nZVI in terms of increased nutrients, C content, and enzymatic activities. On the basis of our results, combined biochar-NP amendments use, specially nH, emerges as a potential post-mining soil restoration strategy.

Polystyrene nanoplastics in soil impair drought priming-induced low temperature tolerance in wheat

Drought priming is known to enhance plant low temperature tolerance, whereas polystyrene nanoplastic contamination exerts detrimental effects on plant growth. This study investigates the less-explored influence of nanoplastic contamination on cold stress tolerance in drought-primed plants. We compared the photosynthetic carbon assimilation, carbohydrate metabolism, reactive oxygen species metabolism, and grain yield between the non-primed and drought-primed wheat grown in both nanoplastic-contaminated and healthy soils. Our results reveal that the beneficial effects of drought priming on photosynthetic carbon assimilation and the efficiency of the “water-water” cycle were compromised in the presence of nanoplastics (nPS). Additionally, nPS exposure disturbed carbohydrate metabolism, which impeded source-to-sink transport of sugar and resulted in reduced grain yield in drought-primed plants under low temperature conditions. These findings unveil the suppression of nPS on drought-primed low-temperature tolerance (DPLT) in wheat plants, suggesting an intricate interplay between the induction of stress tolerance and responses to nPS contamination. The study raises awareness about a potential challenge for future crop production.

Biochar Amendment Alleviates the Combined Effects of Salinity and Drought Stress on Water Productivity, Yield and Quality Traits of Sugar Beet (Beta vulgaris L.)

AbstractSalinity and drought are two commonly occurring major threats to agricultural yields worldwide, including sugar beet production. Therefore, this work aimed to determine the effectiveness of biochar amendment in improving soil health and alleviating the combined effects induced by salinity and drought on morphological, physiological and biochemical responses of sugar beet plants. Two field studies were established at the experimental farm of Fayoum University, Egypt over two successive winter seasons (2019/2020 and 2020/2021). The experiments were carried out in a split-split plot design with triplicate including three factors: 1) soil salinity, with two levels of ECe (&lt; 4 dS m−1 as control and 10 dS m−1) as main-plot factor; 2) deficit irrigation regimes (I100, I80, and I60) representing 100%, 80%, and 60% of crop evapotranspiration (ETc), respectively as sub-plot factor; and 3) biochar amendment with three application levels (B0 as control, B10, and B20) corresponding to 0, 10, and 20 t ha−1, respectively as sub-sub plot factor. Drought and salinity stress induced significant reduction in growth attributes, relative water content, membrane stability, relative chlorophyll content (SPAD), yield and yield components of sugar beet plants. Meanwhile, the contents of sucrose, proline, Na, K, α amino-N were substantially increased with increasing stress severity. The application of biochar amendment improved the soil physical and chemical properties, resulting in better morphological, physiological and biochemical responses of saline-drought stressed sugar beet plants. Biochar at level (B20) produced the highest values of root yield (95.02 t ha−1) and sugar yield (20.24 t ha−1) for full irrigated sugar beet plants in non-saline soil. Moreover, the application of biochar enhanced water productivity for drought and saline stressed sugar beet plants. Biochar amendment could be effectively used as a helpful agro-management strategy in alleviating the detrimental impacts of salinity and water stress on sugar beet plants, improving soil quality and enhancing water productivity to ensure water sustainability and food security, especially in areas of limited water supply.

Mycorrhizal fungi reduce the photosystem damage caused by drought stress on Paris polyphylla var. yunnanensis.

Drought stress (DS) is one of the important abiotic stresses facing cash crops today. Drought can reduce plant growth and development, inhibit photosynthesis, and thus reduce plant yield. In this experiment, we investigated the protective mechanism of AMF on plant photosynthetic system by inoculating Paris polyphylla var. yunnanensis(P.py) with a clumping mycorrhizal fungus (AMF) under drought conditions. The drought environment was maintained by weighing AMF plants and non-AMF plants. The relative water content (RWC) of plant leaves was measured to determine its drought effect. DS decreased the RWC of plants, but AMF was able to increase the RWC of plants. chlorophyll a fluorescence curve measurements revealed that DS increased the OKJIP curve of plants, but AMF was able to reduce this trend, indicating that AMF increased the light absorption capacity of plants. DS also caused a decrease in plant Y(I) and Y(II). ETRI and ETRII, and increased Y(NO) and Y(NA) in plants, indicating that DS caused photosystem damage in plants. For the same host, different AMFs did not help to the same extent, but all AMFs were able to help plants reduce this damage and contribute to the increase of plant photosynthesis under normal water conditions.

Molecular characterization and evaluation of novel management options for Burkholderia glumae BG1, the causative agent of panicle blight of rice (Oryza sativa L.).

Bacterial panicle blight, incited by Burkholderia glumae, has impacted rice production globally. Despite its significance, knowledge about the disease and the virulence pattern of the causal agent is very limited. Bacterial panicle blight is a major challenge in the rice-growing belts of North-western India, resulting in yield reduction. However, the management of B. glumae has become a challenge due to the lack of proper management strategies. Twenty-one BG strains have been characterized using the 16S rRNA and the gyrB gene-based sequence approach in the present study. The gyrB gene-based phylogenetic analysis resulted in geographic region-specific clustering of the BG isolates. The virulence screening of twenty-one BG strains by inoculating the pathogenic bacterial suspension of 1 × 10-8cfu/ml at the booting stage (55 DAT) revealed the variation in the disease severity and the grain yield of rice plants. The most virulent BG1 strain resulted in the highest disease incidence (82.11%) and lowest grain yield (11.12g/plant), and the least virulent BG10 strain resulted in lowest disease incidence of 18.94% and highest grain yield (24.62g/plant). In vitro evaluation of various biocontrol agents and nano copper at different concentrations by agar well diffusion method revealed that nano copper at 1000mg/L inhibited the colony growth of B. glumae. Under net house conditions, nano copper at 1000mg/L reduced the disease severity to 21.23% and increased the grain yield by 20.91% (31.76g per plant) compared to the positive control (COC 0.25% + streptomycin 200ppm). Remarkably, pre-inoculation with nano copper at 1000mg/L followed by challenge inoculation with B. glumae enhanced the activity of enzymatic antioxidants viz., Phenyl ammonia-lyase (PAL), Polyphenol oxidase (PPO) and Peroxidase (POX) and non-enzymatic antioxidant phenol. Additionally, we observed a substantial transcript level upregulation of six defense-related genes to several folds viz., OsPR2, OsPR5, OsWRKY71, OsPAL1, OsAPX1, and OsPPO1 in comparison to the pathogen control and healthy control. Overall, our study provides valuable insights into the potential and practical application of nano copper for the mitigation of bacterial panicle blight, offering promising prospects for commercial utilization in disease management.