As a micronutrient chlorine fulfills several important functions in plant growth and photosynthesis. Former studies indicate an influence of chloride on manganese availability in soils. Due to its high mobility in the vascular system of plants, high chloride concentrations might also be associated with improved manganese transport. Nevertheless, strawberry (Fragaria × ananassa Duch.) is characterized as a chloride-sensitive plant species. To investigate the effect of a moderate chloride nutrition on manganese availability, fruit yield and fruit quality of strawberry, two soil experiments were conducted. In both experiments all plants were fertilized with 3 g K per plant, given in the range of 0-50% (exp. A) or 0-33% (exp. B) as KCl and the remaining K as K2SO4. All treatments were carried out without (control) and with additional manganese fertilization (10 mg Mn per plant). In addition, a nutrient solution experiment was conducted to investigate the effect of a moderate chloride nutrition on manganese uptake and translocation within the plant. Both treatments received potassium at a concentration of 2 mM, given either as 100% K2SO4 or 50% K2SO4 plus 50% KCl. Manganese was supplied at a concentration of 1 µM. In all experiments, macronutrients N, P, and Mg, as well as micronutrients Cu, Zn, B, Fe and Mo were supplied in sufficient amounts. The results of all three experiments indicate that moderate chloride nutrition has no negative effect on fruit yield and fruit quality, regarding sugar and acid concentrations of strawberry fruits. Furthermore, the results of the nutrient solution experiment show that chloride does not improve manganese uptake and translocation within the plant. However, the results of the soil experiments suggest a role of chloride in manganese availability, indicating that a moderate chloride nutrition could compensate insufficient manganese supply.