Plant Cd Uptake Research Articles

Tea biochar-immobilized Ralstonia Bcul-1 increases nitrate nitrogen content and reduces the bioavailability of cadmium and chromium in a fertilized vegetable soil

Pyrolytic biochar (PL-BC, pyrochar) and hydrothermal biochar (HT-BC, hydrochar) derived from branches and leaves of tea plants had different pH, electrical conductivity (EC), total carbon nitrogen content, BET surface area, total pore volume, average pore diameter, and functional groups. HT-BC had a larger specific surface area and more functional groups than PL-BC. Ralstonia Bcul-1 (R-B) was the dominant and functional bacteria in a fertilized vegetable soil supplemented with TBB-immobilized R-B (TBB + R-B). R-B vitality was more closely related to BET surface area, total pore volume, and functional groups of tea-based biochar (TBB: PL-BC and HT-BC). R-B was able to maintain high oxidase activity. R-B and TBB + R-B can increase the activities of urease and peroxidase in vegetable soil playing an essential role in the biotransformation of ammonium nitrogen (NH4+-N) and nitrate nitrogen (NO3−-N). TBB was able to simultaneously increase the content of NO3−-N and NH4+-N, and TBB + R-B also significantly increased NO3−-N content but decreased NH4+-N content in a fertilized vegetable soil. These results indicated that R-B promoted nitrification in the soil, i.e. conversion of NH4+-N into NO3−-N, by enhancing the activities of urease and peroxidase. R-B had high adsorption capacity for cadmium (Cd) and chromium (Cr) (Cd&Cr: Cd and Cr). Moreover, TBB + R-B was able to convert weak acid extractable and reducible Cd&Cr into a more stable residual fraction and oxidizable Cd&Cr. The overall effect of the treatments was to reduce plant uptake of Cd&Cr by cabbage. TBB + R-B significantly promoted R-B growth, changed inorganic nitrogen speciation, increased NO3−-N supply, reduced Cd&Cr bioavailability, and decreased plant tissue Cd&Cr content.

UVB-Pretreatment-Enhanced Cadmium Absorption and Enrichment in Poplar Plants.

The phenomenon of cross adaptation refers to the ability of plants to improve their resistance to other stress after experiencing one type of stress. However, there are limited reports on how ultraviolet radiation B (UVB) pretreatment affects the enrichment, transport, and tolerance of cadmium (Cd) in plants. Since an appropriate UVB pretreatment has been reported to change plant tolerance to stress, we hypothesized that this application could alter plant uptake and tolerance to heavy metals. In this study, a woody plant species, 84K poplar (Populus alba × Populus glandulosa), was pretreated with UVB and then subjected to Cd treatment. The RT-qPCR results indicated that the UVB-treated plants could affect the expression of Cd uptake, transport, and detoxification-related genes in plants, and that the UVB-Pretreatment induced the ability of Cd absorption in plants, which significantly enriched Cd accumulation in several plant organs, especially in the leaves and roots. The above results showed that the UVB-Pretreatment further increased the toxicity of Cd to plants in UVB-Cd group, which was shown as increased leaf malonaldehyde (MDA) and hydrogen peroxide (H2O2) content, as well as downregulated activities of antioxidant enzymes such as Superoxide Dismutase (SOD), Catalase (CAT), and Ascorbate peroxidase (APX). Therefore, poplar plants in the UVB-Cd group presented a decreased photosynthesis and leaf chlorosis. In summary, the UVB treatment improved the Cd accumulation ability of poplar plants, which could provide some guidance for the potential application of forest trees in the phytoremediation of heavy metals in the future.

Coriander (Coriandrum sativum L.) in Combination with Organic Amendments and Arbuscular Mycorrhizal Inoculation: An Efficient Option for the Phytomanagement of Trace Elements-Polluted Soils.

The cultivation of coriander (Coriandrum sativum L.) destined for essential oils production was recently presented as an innovative and economically viable alternative for the phytomanagement of trace elements (TE)-polluted soils. However, Cd accumulation in shoots has proven to be an obstacle in the valorization of the distillation residues and the development of these phytotechnologies. The present study aimed to evaluate the effect of arbuscular mycorrhizal fungus (Funneliformis mosseae) inoculation and organic amendment application on the soil TE bioavailability and plant uptake, as well as on the soil quality and health improvement. The application of compost and sewage sludge improved the growth of coriander and Cd and Zn immobilization in soil, resulting in reduced Cd plant uptake. A synergistic effect of arbuscular mycorrhizal fungi (AMF) inoculation and organic amendments was observed in the decrease in the extractable soil Cd and Zn concentrations, but not in the Cd plant uptake. Despite a significant decrease in Cd accumulation in shoots, coriander retained its accumulative phenotype, with a metal bioconcentration factor close to 1. Furthermore, both the vegetation and the organic amendments improved the soil quality and health by increasing its microbial biomass, as estimated by phospholipid fatty acids, soil enzyme activities (dehydrogenase, phosphatase, β-glucosidase, and cellubiosidase), and the bacterial metabolic function and diversity. The findings demonstrate the potential of C. sativum, particularly in combination with organic amendments and AMF inoculation, for the phytomanagement of TE-polluted soils and soil quality and health improvement.

Supplying amendments alleviates aluminum toxicity and regulates cadmium accumulation by spinach in strongly acidic soils

Al toxicity and Cd pollution are key limiting factors for agricultural production in the acidic soils in China. The application of amendments is an effective and promising measure for remediating strongly acidic Cd-contaminated soils. However, the information on applying amendments for alleviating Al toxicity and regulating plant Cd accumulation is still rare. Here, oyster shell (OS), red mud (RM), hydroxyapatite (HAP), and biochar (BC) at 30 g kg−1 were investigated for alleviating Al toxicity and decreasing Cd accumulation in spinach plants. The results showed that four amendments significantly increased soil pH, and reduced soil exchangeable Al3+ and DTPA-Cd, promoted spinach growth (P < 0.05). Al(OH)30 and Al-HA were the main forms of active Al in soil. The BC and OS were more effective to alleviate Al toxicity but significantly (P < 0.05) increased Cd accumulation in spinach. RM and HAP effectively reduced the uptake of Cd by spinach plants as well as alleviated Al toxicity (P < 0.05). Bivariate correlation analysis and the partial least squares path modeling analysis indicated that soil exchangeable Al3+ was the main limiting factor for biomass production. Our study demonstrated that HAP could significantly alleviate Al toxicity, promote spinach growth, and decrease Cd accumulation in strongly acidic Cd-contaminated soils. Besides, OS and BC effectively alleviated soil Al toxicity leading to promoting the growth of spinach. Compared with CK, RM treatment significantly reduced soil Cd bioavailability (61.2%) and decreased Cd concentration and uptake of spinach plants by 90.0% and 50.7%. These results indicated that RM could be used as an efficient amendment in Cd contaminated.

Effect of soil texture and zinc oxide nanoparticles on growth and accumulation of cadmium by wheat: a life cycle study

Cadmium (Cd) is getting worldwide attention due to its continuous accumulation in agricultural soils which is due to anthropogenic activities and finally Cd enters in food chain mainly through edible plants. Cadmium free food production on contaminated soils is great challenge which requires some innovative measures for crop production on such soils. The current study evaluated the efficiency of zinc oxide nanoparticles (ZnONPs) (0, 150 and 300 mg/kg) on the growth of wheat in texturally different soils including clay loam (CL), sandy clay loam (SCL), and sandy loam (SL) which were contaminated with were contaminated with 25 mg/kg of Cd before crop growth. Results depicted that doses of ZnONPs and soil textures significantly affected the biological yields, Zn and Cd uptake in wheat plants. The application of 300 mg/kg ZnONPs caused maximum increase in dry weights of shoot (66.6%), roots (58.5%), husk (137.8%) and grains (137.8%) in CL soil. The AB-DTPA extractable Zn was increased while Cd was decreased with doses of NPs depending upon soil textures. The maximum decrease in AB-DTPA extractable Cd was recorded in 300 mg/kg of ZnONPs treatment which was 58.7% in CL, 33.2% in SCL and 12.1% in SL soil as compared to respective controls. Minimum Cd concentrations in roots, shoots, husk and grain were found in 300 mg/kg ZnONPs amended CL soil which was 58%, 76.7%, 58%, and 82.6%, respectively. The minimum bioaccumulation factor (0.14), translocation index (2.46) and health risk index (0.05) was found in CL soil with the highest dose of NPs. The results concluded that use of ZnONPs significantly decreased Cd concentration while increased Zn concentrations in plants depending upon doses of NPs and soil textures.

The regulatory role of abscisic acid on cadmium uptake, accumulation and translocation in plants.

To date, Cd contamination of cropland and crops is receiving more and more attention around the world. As a plant hormone, abscisic acid (ABA) plays an important role in Cd stress response, but its effect on plant Cd uptake and translocation varies among plant species. In some species, such as Arabidopsis thaliana, Oryza sativa, Brassica chinensis, Populus euphratica, Lactuca sativa, and Solanum lycopersicum, ABA inhibits Cd uptake and translocation, while in other species, such as Solanum photeinocarpum and Boehmeria nivea, ABA severs the opposite effect. Interestingly, differences in the methods and concentrations of ABA addition also triggered the opposite result of Cd uptake and translocation in Sedum alfredii. The regulatory mechanism of ABA involved in Cd uptake and accumulation in plants is still not well-established. Therefore, we summarized the latest studies on the ABA synthesis pathway and comparatively analyzed the physiological and molecular mechanisms related to ABA uptake, translocation, and detoxification of Cd in plants at different ABA concentrations or among different species. We believe that the control of Cd uptake and accumulation in plant tissues can be achieved by the appropriate ABA application methods and concentrations in plants.

Changes in the m6A RNA methylome accompany the promotion of soybean root growth by rhizobia under cadmium stress

Cadmium (Cd) is the most widely distributed heavy metal pollutant in soil and has significant negative effects on crop yields and human health. Rhizobia can enhance soybean growth in the presence of heavy metals, and the legume–rhizobia symbiosis has been used to promote heavy-metal phytoremediation, but much remains to be learned about the molecular networks that underlie these effects. Here, we demonstrated that soybean root growth was strongly suppressed after seven days of Cd exposure but that the presence of rhizobia largely eliminated this effect, even prior to nodule development. Moreover, rhizobia did not appear to promote root growth by limiting plant Cd uptake: seedlings with and without rhizobia had similar root Cd concentrations. Previous studies have demonstrated a role for m6A RNA methylation in the response of rice and barley to Cd stress. We therefore performed transcriptome-wide m6A methylation profiling to investigate changes in the soybean RNA methylome in response to Cd with and without rhizobia. Here, we provide some of the first data on transcriptome-wide m6a RNA methylation patterns in soybean; m6A modifications were concentrated at the 3′ UTR of transcripts and showed a positive relationship with transcript abundance. Transcriptome-wide m6A RNA methylation peaks increased in the presence of Cd, and the integration of m6A methylome and transcriptome results enabled us to identify 154 genes whose transcripts were both differentially methylated and differentially expressed in response to Cd stress. Annotation results suggested that these genes were associated with Ca2+ homeostasis, ROS pathways, polyamine metabolism, MAPK signaling, hormones, and biotic stress responses. There were 176 differentially methylated and expressed transcripts under Cd stress in the presence of rhizobia. In contrast to the Cd-only gene set, they were also enriched in genes related to auxin, jasmonic acid, and brassinosteroids, as well as abiotic stress tolerance. They contained fewer genes related to Ca2+ homeostasis and also included candidates with known functions in the legume–rhizobia symbiosis. These findings offer new insights into how rhizobia promote soybean root growth under Cd stress; they provide candidate genes for research on plant heavy metal responses and for the use of legumes in phytoremediation.

Microplastics addition reduced the toxicity and uptake of cadmium to Brassica chinensis L.

The coexistence of microplastics (MPs) and toxic metal contaminants in soils is becoming increasingly common, thereby posing serious threat to soil–plant systems. Cadmium (Cd) is the most common metal contaminant in soil and can easily combine with MPs, thereby altering its bioavailability. However, few studies have focused on the co-pollution of MPs and Cd, particularly the complex phytotoxicity caused by their interaction and the effect of co-exposure on Cd uptake in plants. We conducted pot experiments to compare the effects of exposure to polystyrene (PS) and Cd, as well as the effects of co-exposure (PS + Cd), on the physiological characteristics of Brassica chinensis L. and explored the regulatory factors of MPs on Cd uptake in plant tissues. The results showed that plant biomass, photosynthetic parameters, and chlorophyll content significantly decreased (p < 0.05) with increasing PS doses under treatment with MPs alone. Although the negative effects of PS and Cd co-exposure on plants were higher than those of PS alone, however, the addition of MPs reduced the toxicity effects of Cd on plants and decreased the uptake and accumulation of Cd by plants compared with the Cd treatment alone. Furthermore, plants can resist the increased malondialdehyde content and oxidative stress induced by PS and Cd exposure by increasing the activities of superoxide dismutase and peroxidase. Under the PS + Cd treatment, linear models showed that soil organic carbon and sucrase activity were the key variables affecting Cd uptake by plant shoots and roots, respectively. The results of the partial least squares path modeling further showed that PS indirectly affected Cd uptake by B. chinensis by significantly affecting the physicochemical properties of soil, Cd concentration, and enzyme activity. Our results provide a new perspective and an important reference for further understanding the effects of MPs on the bioavailability and fate of heavy metals.

ZAT10 plays dual roles in cadmium uptake and detoxification in Arabidopsis.

Cadmium (Cd) is a harmful heavy metal that is risky for plant growth and human health. The zinc-finger transcription factor ZAT10 is highly conserved with ZAT6 and ZAT12, which are involved in Cd tolerance in plants. However, the definite function of ZAT10 in Cd tolerance remains uncertain. Here, we demonstrated that ZAT10 negatively regulated Cd uptake and enhanced Cd detoxification in Arabidopsis. The expression of ZAT10 in plants is induced by Cd treatment. The zat10 mutant plants exhibited a greater sensitivity to Cd stress and accumulated more Cd in both shoot and root. Further investigations revealed that ZAT10 repressed the transcriptional activity of IRT1, which encodes a key metal transporter involved in Cd uptake. Meanwhile, ZAT10 positively regulated four heavy metal detoxification-related genes: NAS1, NAS2, IRT2, and MTP3. We further found that ZAT10 interacts with FIT, but their regulatory relationship is still unclear. In addition, ZAT10 directly bound to its own promoter and repressed its transcription as a negative feedback regulation. Collectively, our findings provided new insights into the dual functions of ZAT10 on Cd uptake and detoxification in plants and pointed to ZAT10 as a potential gene resource for Cd tolerance improvement in plants.

Biodegradable microplastics impact the uptake of Cd in rice: The roles of niche breadth and assembly process

Biodegradable microplastics (MPs) can impact the accumulation of cadmium (Cd) by plants, however, its mechanisms have not been fully understood. In this study, two biodegradable MPs, polypropylene carbonate (PPC) and polylactic acid (PLA), were used to examine their influences on the uptake of Cd in rice plants. Results showed that PPC significantly reduced the accumulation of Cd in rice root and aerial part, whereas PLA increased the Cd concentrations in rice root. The random forest analysis revealed that the bacterial biomarkers enriched by two MPs were different at genus level. Niche breadths were significantly reduced under Cd stress, and PPC alleviated this environmental pressure for entire bacterial community, whereas PLA reduced the niche breadth for whole community and abundant taxa, which was further verified by co-occurrence network and normalized stochasticity ratio model. The abundant taxa of group PPC were primarily governed by deterministic process while rare taxa were more driven by stochastic process. Structural equation model and Mantel analysis identified that the niche breadth imposed a strong selection on Cd accumulation after co-exposure. This study reveals the underlying mechanism of assembly process and niche breadth of rice rhizosphere microbiome on Cd accumulation by rice plants.

Acidified Carbon with Variable Irrigation Sources Impact on Rice Growth and Yield under Cd Toxic Alkaline Soil Conditions

Cadmium (Cd) is one of the potential carcinogenic toxins for humans, plants, and animals. Higher uptake of Cd in plants causes a significant reduction in productivity that can be remediated using organic amendments. Biochar can absorb Cd and decrease its toxicity. However, the high pH of biochar minimizes its adaptation as an amendment in alkaline soils. As Cd is highly soluble in water, its uptake in rice is a major issue. That is why the current experiment was conducted to examine chemically produced acidified carbon (AC) effectiveness in alleviating Cd-induced stress in rice. There were three levels of Cd (0, 4, and 8 mg kg−1 soil) applied with three levels of AC (0, 0.5, and 1%) for the cultivation of rice irrigated with ground water (GW) and waste water irrigation (WW). Results confirmed that applying 1% AC improved plant height, spike length, and 1000 grains weight over 0% AC under GW and WW irrigations at 8 mg Cd kg−1 soil (8Cd) toxicity. A significant increase in photosynthetic rate, transpiration rate, and stomatal conductance by 1% AC validated its effectiveness in alleviating 8Cd stress in rice under GW and WW. Overall, 1% AC is an effective amendment in alleviating Cd toxicity in rice irrigated with GW and WW at 8Cd. More investigations are recommended at the field level to declare 1% AC as the most effective application rate for mitigating Cd stress in rice.

Physiological and metabolic dynamism in mycorrhizal and non-mycorrhizal Oryza sativa (var. Varsha) subjected to Zn and Cd toxicity: a comparative study.

Arable lands getting contaminated with heavy metals have a very high negative impact on crop plants. The establishment of the mycorrhizal association with crop plants is a sustainable strategy to overcome metal toxicity. The major aim of this study was to analyze mycorrhizae-mediated alterations on the physiology and metabolism of Oryza sativa, as well as the impact of these alterations in the metal tolerance potential of the host on exposure to cadmium (Cd) and zinc (Zn) stresses. For this, 45 d old O. sativa (var. Varsha) plants inoculated with Claroideoglomus claroideum were exposed to 1.95 g Zn kg-1 soil and 0.45 g Cd kg-1 soil. Mycorrhization significantly increased shoot weight, root weight, moisture content, and chlorophyll biosynthesis under Cd and Zn stresses. Mycorrhization mitigated the oxidative stress elicited in O. sativa by the elevated Cd and Zn content, and it aided in maintaining the metabolite's level and rate of photosynthesis as compared to non-mycorrhizal plants. The circular-shaped unique structures seen as opening on the leaf surface of non-mycorrhizal plants under Zn stress, possibly for the emission of volatile compounds synthesized as a result of Zn stress, have a great chance of leaf tissue destruction. This structural modification was characterized in the case of Zn stress and not in Cd stress and can lead to the reduction of photosynthesis in O. sativa exposed to Zn stress. The reduction in oxidative stress could be correlated to the reduced uptake and transport of Cd and Zn ions in mycorrhizal plants. The exudation of tributyl acetyl citrate, 3-beta-acetoxystigmasta-4,6,22-triene, and linoleic acid from the mycorrhizal roots of rice plants has a crucial role in the stabilization of metal ions. This study proposes mycorrhization as a strategy to strengthen the Cd and Zn stress tolerance level of rice plants by regulating the physiology and metabolomics of the host plant.

Arbuscular mycorrhizal fungi reduce potassium, cadmium and ammonium losses but increases nitrate loss under high intensity leaching events

BackgroundNutrients and heavy metals can be lost from soils via leaching, and arbuscular mycorrhizal fungi (AMF) can influence these events. Soil column experiments were carried out to examine whether leaching intensity and AMF can alter nutrient and Cd uptake in white clover plants and the extent of their losses through leaching.ResultsThe presence of AMF significantly increased shoot and total biomass, as well as increased N, P, Cu and Zn uptake independent of water amount applied; while root P and Cu uptakes were promoted by AMF at any water amount treatments. Higher water amounts led to reductions in total N, K and Zn uptake for AMF-colonized plants in comparison to moderate water amount treatments. In the absence of AMF, white clover at low water amount treatment exhibited maximal root Cd uptake. At high water amount treatments, the presence of AMF significantly decreased leachate volumes and the amount of leached NH4+, K and Cd while AMF significantly increased the amounts of leached NO3−.ConclusionsOverall we found that AMF-colonized white clover plants reduced NH4+, K and Cd loss from soils but increased the risk of NO3− loss under high intensity leaching conditions.

Advances in Genes-Encoding Transporters for Cadmium Uptake, Translocation, and Accumulation in Plants.

Cadmium (Cd) is a heavy metal that is highly toxic for plants, animals, and human beings. A better understanding of the mechanisms involved in Cd accumulation in plants is beneficial for developing strategies for either the remediation of Cd-polluted soils using hyperaccumulator plants or preventing excess Cd accumulation in the edible parts of crops and vegetables. As a ubiquitous heavy metal, the transport of Cd in plant cells is suggested to be mediated by transporters for essential elements such as Ca, Zn, K, and Mn. Identification of the genes encoding Cd transporters is important for understanding the mechanisms underlying Cd uptake, translocation, and accumulation in either crop or hyperaccumulator plants. Recent studies have shown that the transporters that mediate the uptake, transport, and accumulation of Cd in plants mainly include members of the natural resistance-associated macrophage protein (Nramp), heavy metal-transporting ATPase (HMA), zinc and iron regulated transporter protein (ZIP), ATP-binding cassette (ABC), and yellow stripe-like (YSL) families. Here, we review the latest advances in the research of these Cd transporters and lay the foundation for a systematic understanding underlying the molecular mechanisms of Cd uptake, transport, and accumulation in plants.

Effects of Amendments and Indigenous Microorganisms on the Growth and Cd and Pb Uptake of Coriander (Coriandrum sativum L.) in Heavy Metal-Contaminated Soils.

Heavy metal (HM) contamination of soils is a worldwide problem with adverse consequences to the environment and human health. For the safe production of vegetables in contaminated soil, efficient soil amendments need to be applied such as nano-hydroxyapatite (n-HAP) and poly γ-glutamic acid (γ-PGA), which can mitigate heavy metal uptake and enhance crop yield. However, the combined effects of soil amendments and indigenous microorganisms (IMOs) on HMs immobilisation and accumulation by crops have received little attention. We established a pot experiment to investigate the effects of IMOs combined with n-HAP and γ-PGA on coriander (Coriandrum sativum L.) growth and its Cd and Pb uptake in two acidic soils contaminated with HMs. The study demonstrated that applying n-HAP, with and without IMOs, significantly increased shoot dry biomass and reduced plant Cd and Pb uptake and diethylenetriaminepentaacetic acid (DTPA) extractable Cd and Pb concentrations in most cases. However, γ-PGA, with and without IMOs, only reduced soil DTPA-extractable Pb concentrations in slightly contaminated soil with 0.29 mg/kg Cd and 50.9 mg/kg Pb. Regardless of amendments, IMOs independently increased shoot dry biomass and soil DTPA-extractable Cd concentrations in moderately contaminated soil with 1.08 mg/kg Cd and 100.0 mg/kg Pb. A synergistic effect was observed with a combined IMOs and n-HAP treatment, where DTPA-extractable Cd and Pb concentrations decreased in slightly contaminated soil compared with the independent IMOs and n-HAP treatments. The combined treatment of γ-PGA and IMOs substantially increased shoot dry biomass in moderately contaminated soil. These results indicate that solo n-HAP enhanced plant growth and soil Cd and Pb immobilisation, and mitigated Cd and Pb accumulation in shoots. However, the combination of n-HAP and IMOs was optimal for stabilising and reducing HMs’ uptake and promoting plant growth in contaminated soil, suggesting its potential for safe crop production.

Possibility of using combined compost–attapulgite for remediation of Cd contaminated soil

Soil contamination by cadmium (Cd) is a critical global environmental issue that urgently needs to be solved. In this study, compost, attapulgite, and their combination were used for remediation of Cd-contaminated soil. Soil physicochemical properties, enzyme activities and plant uptake of Cd were investigated to evaluate the remediation effectiveness. Attapulgite and combined compost–attapulgite significantly increased the soil pH, while compost and combined compost–attapulgite induced the larger increase in soil organic matter (SOM) and available P and K. The catalase, urease, and sucrase activities in the soil had considerably improved, and those activities in the soil treated with compost–attapulgite increased by 2.04, 1.15 and 0.32 times, respectively, compared to untreated soil. The decrease of CaCl2–Cd content in the soil treated with combined compost–attapulgite was most pronounced, with a decline of 96.38% compared to untreated soil. Meanwhile, combined compost–attapulgite led to the decrease in exchangeable and reducible Cd from 0.70 to 0.64 mg kg−1 and from 0.47 to 0.43 mg kg−1, respectively, and an increase of residual Cd from 0.06 to 0.16 mg kg−1. The combination of compost and attapulgite promotes Cd immobilization through adsorption, complexation, coprecipitation, and ion exchange. The obvious Cd immobilization effectiveness of combined compost–attapulgite resulted in the lowest pakchoi (Brassica chinensis L.) uptake of Cd among treatments. These findings provided an alternative strategy for improving soil quality, reducing Cd bioavailability through combined use of compost and attapulgite as soil amendment in Cd contaminated soil.

Increasing phosphorus inhibits the retention and prevention of cadmium by iron plaque and promotes cadmium accumulation in rice plants

Iron (Fe) plaque is an important component of rice roots because it influences the uptake and transport of cadmium (Cd) in rice. In this study, a hydroponic experiment was developed to investigate the influence of phosphorus (P) on the formation of iron plaque on the root surface as well as the influence on Cd uptake in rice plants. Three important results were obtained, as follows. (1) During the formation of iron plaque induced by exogenous Fe, P supply was beneficial for the iron plaque formation, but it restrained the Cd retention capability, resulting in a decrease in Cd in iron plaque by 35.48–61.93%, and leading to an increase in Cd in rice roots from 72.13 mg kg−1 to 112.78 mg kg−1 (2) After the iron plaque induction, the formation of iron plaque was inhibited by P supply, resulting in the amount of iron plaque decreasing by 18.46–54.57%, and the Cd in iron plaque decreased by 3.93–31.78%. Then, the Cd retention capability and the prevention effect simultaneously decreased, and as a result, the Cd in rice roots increased from 100.83 mg kg−1 to 146.03 mg kg−1 (3) Without exogenous Fe induction, P sufficiency continued to increase the amount of iron plaque and decrease the Cd in iron plaque, and increase the Cd in rice plants. These results suggested that P supply increases the amount of iron plaque, which is ineffective for Cd retention, such as non-reddish-brown iron plaque (NIP), and then decreases the capacity of iron plaque to retain Cd. Additionally, the P supply decreased the amount of formed iron plaque, causing the decreasing prevention effect. Therefore, excessive application of P fertilizer should be avoided in Cd-contaminated paddy fields.

The Potential of Nitrogen Applied Rapeseed (Brassica napus L) in Remediation of Cadmium Polluted Soil

This study aims to determine the Cd accumulation levels of rapeseed (Brassica napus L.) based on nitrogen (N) (0, 100, 200 and 400 mg kg-1) and Cd (0, 1.5, 3 and 6 mg kg-1) applications to the soil on pot trials conducted under controlled environmental conditions. The highest chlorophyll content was obtained at 400 mg N kg-1 without Cd. The highest dry weight amount in rapeseed was obtained with the application of 100 mg N kg-1 × 1.5 mg Cd kg-1, however, the highest rates of both N and Cd resulted in the lowest dry weight. In 400 mg N kg-1 × 6 mg Cd kg-1 (131 mg Cd kg-1) and 200 mg N kg-1 × 6 mg Cd kg-1 (113.80 mg Cd kg-1) applications, Cd concentration of the rapeseed was determined to be higher than critical Cd hyperaccumulation level. The capacity of Cd uptake and accumulation in rapeseed shoots were enhanced with N applications, thus promoting the phytoextraction of Cd from Cd-contaminated soils. Cadmium rates did not affect Fe, Cu, Mn and Zn uptake levels when N was not applied. However, Cd ratios increased the microelement uptake levels, except Zn, when applied to 100 and 200 N kg-1 ratios as compared to control plants. Eventually, increase in Cd uptake of plants with N fertilization is possible; however, excessive N rates may not result in an increase in rapeseed biomass.

WRKY74 regulates cadmium tolerance through glutathione-dependent pathway in wheat.

Cadmium (Cd) is a toxic heavy metal to plants and human health. Ascorbate (ASA)-glutathione (GSH) synthesis pathway plays key roles in Cd detoxification, while its molecular regulatory mechanism remains largely unknown, especially in wheat. Here, we found a WRKY transcription factor-TaWRKY74, and its function in wheat Cd stress is not clear in previous studies. The expression levels of TaWRKY74 were significantly induced by Cd stress. Compared to control, the activities of GST, GR, or APX were significantly increased by 1.55-, 1.43-, or 1.75-fold and 1.63-, 2.65-, or 2.30-fold in shoots and roots of transiently TaWRKY74-silenced wheat plants under Cd stress. Similarly, the contents of hydrogen peroxide (H2O2), malondialdehyde (MDA), GSH, or Cd were also significantly increased by 2.39- or 1.25-fold, 1.54- or 1.20-fold, and 1.34- or 5.94-fold in shoots or roots in transiently TaWRKY74-silenced wheat plants, while ASA content was decreased by 47.4 or 43.3% in shoots, 10.7 or 6.5% in roots in these silenced wheat plants, respectively. Moreover, the expression levels of GSH, GPX, GR, DHAR, MDHAR, and APX genes, which are involved in ASA-GSH synthesis, were separately induced by 2.42-, 2.16-, 3.28-, 2.08-, 1.92-, and 2.23-fold in shoots, or by 10.69-, 3.33-, 3.26-, 1.81-, 16.53-, and 3.57-fold in roots of the BSMV-VIGS-TaWRKY74-inoculated wheat plants, respectively. However, the expression levels of TaNramp1, TaNramp5, TaHMA2, TaHMA3, TaLCT1, and TaIRT1 metal transporters genes were decreased by 21.2-76.3% (56.6%, 59.2%, 76.3%, 53.6%, 35.8%, and 21.2%) in roots of the BSMV-VIGS-TaWRKY74-inoculated wheat plants. Taken together, our results suggested that TaWRKY74 alleviated Cd toxicity in wheat by affecting the expression of ASA-GSH synthesis genes and suppressing the expression of Cd transporter genes, and further affecting Cd uptake and translocation in wheat plants.

Exogenous melatonin mitigates cadmium toxicity through ascorbic acid and glutathione pathway in wheat

Cadmium (Cd) is a dispensable element that can be absorbed by crops, posing a threat to human health through the food chains. Melatonin (MT), as a plant growth regulator, has been used to alleviate Cd toxicity in many plant species; however, the underlying molecular mechanisms responsible for Cd toxicity in wheat are still poorly understood. In this study, the suitable exogenous MT concentration (50 μM) was screened to mitigate Cd toxicity of wheat plants by increasing the plant height, root length, fresh or dry weight and chlorophyll content, or decreasing the malondialdehyde (MDA) content. In addition, MT application significantly increased ascorbic acid (ASA) and glutathione (GSH) content by reducing ROS production, especially in roots, further decreasing Cd content in fraction of organelles. Moreover, the expression levels of ASA-GSH synthesis genes, APX, GR, and GST were significantly increased by 171.5%, 465.2%, and 256.8% in roots, respectively, whereas GSH, DHAR, or MDHAR were significantly decreased by 48.5%, 54.3%, or 60.0% in roots under MT + Cd stress. However, the expression levels of Cd-induced metal transporter genes TaNramp1, TaNramp5, TaHMA2, TaHMA3, and TaLCT1 were significantly decreased by 53.7%, 50.1%, 86.5%, 87.2%, and 94.5% in roots under MT + Cd stress compared with alone Cd treatment, respectively. In conclusion, our results suggesting that MT alleviate Cd toxicity in wheat by enhancing ASA-GSH metabolism, suppressing Cd transporter gene expression, and regulating Cd uptake and translocation in wheat plants.