Plant Resins Research Articles

Comparative metabolomic reveals chemotaxonomic markers of resin fossils for identification of botanical origins

Fossil resins are often used to indicate the botanical origins of ancient plants through their secondary metabolites. However, the existing chemotaxonomy of fossil resins is limited in its ability to distinguish them at phylum, family, and genus levels using small amount of samples. Herein an advanced head-space solid phase microextraction- comprehensive two-dimensional gas chromatography- time-of-flight mass spectrometer platform was established to analyze the complex compositions of resin fossils derived from ancient gymnosperms (e.g., Eocene Cupressaceae, Cretaceous Araucariaceae) and angiosperms (Miocene Dipterocarpaceae and Miocene Hymenaea). The secondary metabolites including 83 monoterpenes, 186 sesquiterpenes, 84 diterpenes, and 43 n-alkanes were annotated and then chemically fingerprinted for the first time. Prior to conducting chemical composition classification, this study tested the vitrinite reflectance of resin-bearing rocks and Tmax of the ambers to demonstrate their similar thermal maturity. After that, chemotaxonomic markers were disclosed using multivariate statistical analysis to identify the botanical origins of resin fossils, and four identification rules were concluded. Gymnosperm and angiosperm resins can be distinguished by the relative content of monoterpenes and diterpenes. Eocene Cupressaceae and Cretaceous Araucariaceae resins can be identified by ten chemotaxonomic markers which were screened out by orthogonal partial least-squares regression. Miocene Hymenaea and Dipterocarpaceae resins have different combinations of chemical superclasses, with sesqui- and di-terpenes being the major compositions of Hymenaea resins, and mono- and sesqui- terpenes accounting for most of the compositions of Dipterocarpaceae resins. Specific chemotaxonomic markers can identify Hymenaea mexicana and Hymenaea protera resins. As the chemical components of fossil resins were determined by the genes present in their botanical origins, the chemotaxonomic markers revealed in this study have a genetic basis. These findings provide a comprehensive database of secondary metabolites from ancient resin plants, which can be utilized for further research on their corresponding paleoenvironment and to clarify their phylogeny.

Cuisine in transition? Organic residue analysis of domestic containers from 9th-14th century Sicily.

From the 9th to 14th centuries AD, Sicily experienced a series of rapid and quite radical changes in political regime, but the impact of these regime changes on the lives of the people that experienced them remains largely elusive within the historical narrative. We use a multi-faceted lipid residue approach to give direct chemical evidence of the use of 248 everyday domestic ceramic containers from Islamic and post-Islamic contexts in western Sicily to aid our understanding of daily habits throughout this period of political change. A range of commodities was successfully identified, including animal fats, vegetable products, fruit products (potentially including wine) and plant resins. The study highlights the complexity of residues in early medieval Mediterranean society as, in many cases, mixtures of commodities were observed reflecting sequential cooking events and/or the complex mixtures reflective of medieval recipes. However, overall, there were no clear changes in the composition of the residues following the imposition of Norman control over the island and through subsequent periods, despite some differences between urban centres and rural sites. Thus, lending to the idea that post-Islamic populations largely flourished and benefited from the agricultural systems, resources and recipes left by their predecessors.

In silico and in vitro anti-inflammatory study of phenolic compounds isolated from Eucalyptus maculata resin

Plant resins are rich in bioactive compounds with high medicinal values. However, the chemistry and anti-inflammatory activity of the resins produced by trees of the genus Eucalyptus were scarcely investigated. The inflammatory targets cyclooxygenase-1 (COX-1), COX-2, TNF-, NF-B, and NO were significantly inhibited by the methanolic extract of Eucalyptus maculata kino resin (EME) and its CH2Cl2 soluble fraction (MCF). Sakuranetin (C1), (E)-cinnamic acid (C2), kaempferol 7- methyl ether (C3), 7-O-methyl aromadendrin (C4), and 1,6- dicinnamoyl-O-α-D-glucopyranoside (C5) were isolated from MCF. Three compounds (C1, C2, and C4) showed potent in vitro COX-1 inhibition, while C5 inhibited COX-2, TNF-α, NF-κB, and NO significantly. An in-silico study revealed that C5 had the highest binding affinity to the active site in COX-2 with binding energy score (S) of -14.85 kcal/mol, better than celecoxib (COX-2 inhibitor). In conclusion, 1,6-dicinnamoyl-O-α-D-glucopyranoside (C5) could be investigated further in the search for anti-inflammatory agents.

Propolis and Their Active Constituents for Chronic Diseases.

Propolis is a mass of chemically diverse phytoconstituents with gummy textures that are naturally produced by honeybees upon collection of plant resins for utilization in various life processes in beehives. Since ancient times, propolis has been a unique traditional remedy globally utilized for several purposes, and it has secured value in pharmaceutical and nutraceutical areas in recent years. The chemical composition of propolis comprises diverse constituents and deviations in the precise composition of the honeybee species, plant source used for propolis production by bees, climate conditions and harvesting season. Over 300 molecular structures have been discovered from propolis, and important classes include phenolic acids, flavonoids, terpenoids, benzofurans, benzopyrene and chalcones. Propolis has also been reported to have diverse pharmacological activities, such as antidiabetic, anti-inflammatory, antioxidant, anticancer, immunomodulatory, antibacterial, antiviral, antifungal, and anticaries. As chronic diseases have risen as a global health threat, abundant research has been conducted to track propolis and its constituents as alternative therapies for chronic diseases. Several clinical trials have also revealed the potency of propolis and its constituents for preventing and curing some chronic diseases. This review explores the beneficial effect of propolis and its active constituents with credible mechanisms and computational studies on chronic diseases.

The induction techniques of resinous agarwood formation: A review

Agarwood is an aromatic, resinous plant that contains volatile, fragrant sesquiterpenes and phenylethylchromones found in wood tissue near damaged or diseased Aquilaria sp. However, natural agarwood production from young, healthy plants is insufficient to meet global market demand. Therefore, this article evaluates the impact of agarwood production methods on the success of the agarwood industry. Recent advances in artificial agarwood induction technology, including mechanical, bio-inoculants, chemical inducers, combinations of biological and chemical inducers, and in vitro plant tissue culture, have resulted in efficiently induced agarwood resin compared with conventional methods. Although interdisciplinary approaches have many advantages and disadvantages, it is encouraging that chemical inducers have a wide distribution in the trunk of Aquilaria sp. Also, able to consistently produce high quality agarwood resin. It can be concluded that different forms of inducers can be used to develop excellent quality agarwood in young plantation trees, equivalent to wild agarwood.

Comprehensive estrogenic/anti-estrogenic, anticancer, mutagenic/anti-mutagenic, and genotoxic/anti-genotoxic activity studies on chemically characterized black poplar and Eurasian aspen propolis types

Propolis is mainly composed of plant resins, and its type is named according to the primary plant origin in its composition. Identification of propolis botanical origin is essential for predicting and repeating its pharmacological activity because of the variations in chemical composition. This study aimed to compare chemical composition of black poplar (Populus nigra L.) type-propolis (PR1 and PR2) and Eurasian aspen (P. tremula L.)-type propolis (PR3) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique and to evaluate their biological activity profiles. According to LC-MS/MS results, in addition to marked caffeic acid phenethyl ester content in PR1 and PR2, flavonoid aglycones such as pinocembrin, chrysin, pinobanksin, and galangin were found to be dominant in these samples. On the other hand, PR3 contained relatively high concentrations of phenolic acids such as ferulic acid, p-coumaric acid, and trans-cinnamic acid. The anti-estrogenic activity test showed that PR2 exerted the highest anti-estrogenic activity by inhibiting cell proliferation by 44.6%. All propolis extracts showed anticancer activity, which was justified by decreasing activity on the 3D spheroid size in a concentration-dependent manner. Besides, all extracts showed moderate or potent antimutagenic activity in Salmonella typhimurium TA98 and TA100 strains with and without metabolic activation, respectively. In addition, the Comet assay results revealed that propolis extracts have a geno-protective effect against H2O2-induced DNA damage in CHO-K1 cells at 0.625 and 1.25 μg/mL concentrations. Overall, the result of this study may help in preparing standardized propolis extracts and developing products with defined pharmacological benefits in the food supplements industry.

Thermal and mechanical analysis of thermal power plant ashes, cement and resin composites

This study used thermal power plant fly ash (FA) and bottom ash (BA) as fill materials and investigated the thermal properties of composites made of cement and pine tree resin binders. For each ash group, 36 samples were prepared by adding 50% cement and 1% to 2% resin as binders. i) FA samples had 23.18%, 22.99%, and 77.01% lower density, thermal conductivity, and compressive strength than BA samples, respectively. FA samples had 9.42% higher porosity than BA samples. ii) FA (0.177 W/mK) and BA (0.221 W/mK) samples with resin and cement had the lowest heat transfer coefficients. iii) FA (14.46 MPa) and BA (36.96 MPa) samples (resin + cement binder) had the highest compressive strength values.

Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method

Background: Extracted from the Indian branching tree Boswellia serrata, boswellic acids derived from the plant's resin hold promise in addressing inflammation, arthritis, and hyperlipidemia. Objective: In this study, the mutagenicity of B. serrata (Boswegex®) is evaluated. Materials and Methods: The potential genotoxic and mutagenic effects of Boswellia serrata's aqueous ethanolic extract, known as Boswegex®, were explored using a bacterial reverse mutation assay against Salmonella strains TA98, TA100, TA102, TA1535, and TA1537. Different concentrations ranging from 500 to 6000 μg/plate were tested using both Method 1 (Standard plate incorporation assay) and Method 2 (pre-incubation assay), with DMSO (Dimethyl sulfoxide) as the solvent control. The positive control, 2-aminoanthracene, was used alongside a metabolic activator (S-9 fraction). Given the prolonged use of supplements, an assessment for mutagenicity is vital. In this context, the mutagenicity of B. serrata (Boswegex®) was evaluated through the in vitro Bacterial Reverse Mutation Test, employing both the Standard Plate Incorporation Assay and the pre-incubation assay. Results: Notably, none of the mutagenic assays revealed a mutagenicity increase over background levels. Conclusion: These findings collectively indicate that Boswellia serrata (Boswegex®) does not exhibit mutagenic properties.

Sulfurized diterpenoids in amber as diagenetic indicators of sulfate-reducing processes in past depositional environments.

Two novel compounds isolated from an amber sample from the Santonian of Piolenc (Vaucluse, SE France) were identified using nuclear magnetic resonance and high-resolution mass spectrometry as sulfurized analogues of diterpenic acids from the isopimaric series originating from ancient conifers possibly related to the Cupressaceae family. These two compounds are members of a diterpenoid series corresponding to early diagenetic transformation products of resin diterpenoids. They were likely formed once plant resin comes into contact with reduced sulfur species originating from bacterial sulfate reduction occurring in anaerobic settings such as mangroves or marshes. They represent the first evidence of sulfurization processes affecting plant resin prior to diagenetic transformation into amber. Given their mode of formation, these compounds may be used as indicators of sulfate-reducing processes in past depositional environments.

Determination of Inhibition Effect of Propolis Extract on Watermelon mosaic virus in Edible Seed Squash

Edible seed squash (Cucurbita pepo L, ESS), which is grown for its seeds to consume as a snack, has a very important place in vegetable production in Türkiye. Viruses are one of the most problems in the cultivation of ESS in the country. One of these diseases is Watermelon mosaic virus (WMV, Potyvirus, Potyviridae). Also, it’s very common and devastating on other cucurbits worldwide. Propolis, which is composed mainly by the plant resins and exudates that honey bees gather, showed antiviral activity against plant and human viruses. In this study, the potential of propolis to control WMV was investigated by taking advantage of these antimicrobial properties of propolis. For this purpose, propolis extracted with 95% ethanol and diluted in distilled water to obtain different concentrations as 2, 4, 6, 8 and 10% were used. Effects of propolis against WMV were determined by in vitro and in vivo studies. Except of 2, and 4%, all concentrations caused to symptoms reductions of the infection, in all studies. In the result of in vitro studies, the ratios of healthy plants were calculated as 10, 20, 40% and 20, 30, 50% after one and two hours, respectively. In vivo, by spraying concentrations of 6, 8 and 10% before inoculation obtained healthy plants as 20, 40 and 60% after one-hour period, and as 20, 30 and 50% after two-hour period, while the extracts sprayed after inoculation, ratios of healthy plants as 10, 20 and 30% after one-hour period, and as 10, 20 and 20% after two-hour period. According to the results of the study, it was determined that applications of different propolis concentrations had the potential to reduce WMV infection, and these results should be supported by field trials.

BEE PROPOLIS USE IN BIOTIC AND ABIOTIC COMPOSITIONS AND ITS BIOLOGICAL ORIGIN FOR ANTICANCER MEDICINES

Propolis is a resinous compound with a balsamic aroma and a sticky consistency that is harvested from the exudates, flowers, and buds of several plants. These biological effects are tied to the phenolic chemicals found in propolis, the structure and concentration of which change depending on the region of production, the variety of plant resins available, the genetic makeup of the queen bee, the technique of production, and the time of year. Biomolecules isolated from plants have the potential to serve as the basis for anticancer medication prototypes with the ability to suppress tumour growth. The structure and concentration of propolis' phenolic compounds vary on the place of production, the range of plant resins accessible, the queen bee's genetic makeup, the manufacturing method, and the season. Plant biomolecules may be used to develop anticancer drugs that inhibit tumour growth. Tropical and subtropical regions are good for bioprospecting due of their biodiversity. Learning about these chemicals' efficacy is crucial since they could be utilised as adjuvants with other drugs or as new cancer treatments. Bee propolis is an important anticancer medication, but more preclinical and clinical research is needed to determine its selectivity, efficacy, and safety. We emphasised on the importance of bee propolis as an anticancer drug and focusing on the chemical components and biological processes.

Gastroprotective and anti-Helicobacter pylori potentials of essential oils from the oleoresins of Araucaria bidwillii and Araucaria heterophylla

Plant resins or oleoresins comprise a chemically complex mixture of different classes of compounds. Oleoresin of the genus Araucaria combines essential oil (EO) and resin. It possesses gastroprotective, cytotoxic, and timicrobial, antipyretic, and anti-inflammatory activities. The study aimed to investigate the EOs from the oleoresins of two Araucaria species, A. bidwillii and A. heterophylla, chemically and biologically for their gastroprotective, anti-inflammatory, antioxidant, and anti-Helicobacter pylori potentials. The chemical composition of both species cultivated in Egypt was analyzed with GC-MS and compared with those cultivated abroad using principal component analysis (PCA). There were 37 and 17 secondary metabolites identified in A. heterophylla and A. bidwillii, respectively. The EOs of both species showed a pronounced inhibitory effect on Helicobacter pylori activity in vitro. The gastroprotective effect was assessed in vivo using ethanol-induced gastric ulcer model in rats. Inflammatory cytokines, oxidative stress, and the nuclear factor-kappa B (NF-κB) biomarkers were assessed in the stomach tissues. The ulcer index and percentage of ulcer protection were determined. Stomach sections were examined histopathologically by staining with (H/E) and periodic acid Schiff (PAS). Moreover, the proliferative index was determined using the Ki-67 immunostaining. The treatment of rats with EOs (50, 100, and 200 mg/kg, orally) 1 hour prior to ethanol administration showed promising gastroprotective, anti-inflammatory, and antioxidant potentials. These findings declared the gastroprotective role played by both EOs with the superiority of A. bidwillii over A. heterophylla via modulation of oxidative stress/NF-κB/inflammatory cytokines. Their use can be recommended to protect against the recurrence of peptic ulcers.

Removal of chlorine, microparticles and water from high-viscosity liquids by a sand filter coupling hydrocyclone

Separation energy consumption in industrial production accounts for 10–15 % of global energy consumption. Nonthermal separation represented by sand filtration can open a new door to separation energy savings. Traditional sand filters cannot be regenerated completely, which limits their efficiency. A sand filter coupling hydrocyclone (SFCH) was proposed to enhance regeneration. The performance of SFCHs with different separation particle sizes was systematically analyzed, as indicated by turbidity, water, and chlorine in a pilot-scale experiment. The larger the sand particle size of the SFCH was, the longer it took to reach saturation. The efficiency and cycle were balanced by combining SFCHs with particle sizes of 1–2 mm and 0.5–1 mm. The average turbidity, water and chlorine decreased from 793 NTU, 1439 mg/L, and 126 mg/L to 155 NTU, 389 mg/L, and 88 mg/L, respectively, with a cycle of 88 h. Under the same conditions, the effluent turbidity of the SFCH was half of that of the original disc centrifuge, which indicates that the life of the downstream alumina bed was doubled. Almost 260 t/year alumina consumption can be reduced in a 200,000 tons/year-capacity petroleum resin plant, saving 600,000 USD/year, mainly due to the extension of the service life of the alumina bed.

Stingless bee (Apidae: Meliponini) foraging and predation at trunk resin sources: Rare observations captured with microcontroller-based camera traps in a lowland dipterocarp forest

An intriguing yet little studied aspect of social insect foraging is the use of resources other than food. We are interested in the collection of plant resins for nest construction and defense by tropical stingless bees. However, direct observations of stingless bee foraging and potential predation activities by natural enemies at resin sources are particularly rare and therefore require a trade-off between observation time and the number of sources observed. We used affordable remote microcontroller-based camera traps to enable for longer durations of continuous and simultaneous monitoring of resin foraging at multiple resin sources in an undisturbed lowland dipterocarp rainforest in Brunei Darussalam (Borneo). Analysis of photos from camera traps shows that stingless bee visitation to resin sources was uncommon at resin wounds in the forest understory (27.3%). Bees visiting wounds displayed a propensity for short and regular resin foraging bouts of up to a few days to particular resin sources. Where there were encounters between stingless bees and natural enemies, i.e., assassin bugs at resin sources, there was a 100% predation success rate (n = 4). Our study suggests that microcontroller-based camera traps complement or may even replace in-person field observations, in particular for observations of organisms or interactions occurring at low abundance. They allow for systemically collected observations which can form the basis for hypothesis-driven research as part of “next-generation natural history”.

Developing Strategies to Help Bee Colony Resilience in Changing Environments.

Climate change, loss of plant biodiversity, burdens caused by new pathogens, predators, and toxins due to human disturbance and activity are significant causes of the loss of bee colonies and wild bees. The aim of this review is to highlight some possible strategies that could help develop bee resilience in facing their changing environments. Scientists underline the importance of the links between nutrition, microbiota, and immune and neuroendocrine stress resistance of bees. Nutrition with special care for plant-derived molecules may play a major role in bee colony health. Studies have highlighted the importance of pollen, essential oils, plant resins, and leaves or fungi as sources of fundamental nutrients for the development and longevity of a honeybee colony. The microbiota is also considered as a key factor in bee physiology and a cornerstone between nutrition, metabolism, growth, health, and pathogen resistance. Another stressor is the varroa mite parasite. This parasite is a major concern for beekeepers and needs specific strategies to reduce its severe impact on honeybees. Here we discuss how helping bees to thrive, especially through changing environments, is of great concern for beekeepers and scientists.

Textual research on varieties of Draconis Sanguis

This paper reviewed the historical evolution of the varieties of Draconis Sanguis in traditional Chinese medicine(TCM) and discussed several doubts. Draconis Sanguis used in ancient Europe and Arabia was derived from Dracaena plants, and that originating from Southeast Asia entered the market in the 16 th century. Draconis Sanguis was introduced into China in the 5 th century at the latest and was once mixed with shellac for use. Draconis Sanguis in the Tang Dynasty and before was the resin of Dracaena plants. Scholars in the Song Dynasty have known that Draconis Sanguis came from the resin of tall trees, but their understanding of origin plants was inconsistent with the facts. The origin of Draconis Sanguis in the Song Dynasty was basically determined to be Mirbat(Maliba), Cengtan, and Somali, as well as Socotra Archipelago. About 1371-1416, Draconis Sanguis prepared from Daemonorops draco was imported into China, and was recorded earlier in The Overall Survey of the Ocean's Shores(Ying Ya Sheng Lan) and Code of Great Ming Dynasty(Da Ming Hui Dian). Draconis Sanguis prepared from Dracaena plants was still authentic for a long time after the import of that from D. draco into China. During the Ming and Qing Dynasties, Dian Zhi(1625), a lost edition of Materia Medica in Southern Yunnan(Dian Nan Ben Cao), Textual Research on Reality and Titles of Plants(Zhi Wu Ming Shi Tu Kao), and other local chronicles recorded that a new type of Draconis Sanguis(Mu Xue Jie) was produced in Yuanjiang, Yunnan province. The New Yunnan Chronicles of the Republic of China recorded the production of another type of Draconis Sanguis(Qi Lin Jie) in Xishuangbanna. However, the authenticity of the above two types has been difficult to confirm. In modern times, Draconis Sanguis prepared from D. draco gradually became the mainstream variety. In the 1970 s, Dracaena cochinchinensi was found in Yunnan and other provinces, and Draconis Sanguis from D. cochinchinensi was developed. This study is expected to provide a solid and reliable literature support for the research and development of Draconis Sanguis, enrich historical materials, and provide new clues for follow-up research.

Nesting Biology and Ecology of a Resin Bee, Megachile cephalotes (Megachilidae: Hymenoptera).

We report the nesting biology and ecology of Megachile cephalotes Smith, 1853 for the first time in Pakistan. Wooden and bamboo trap nests were deployed at three different locations in Bahawalpur district, Pakistan, from January 2020 to May 2021. A total of 242 nests of M. cephalotes were occupied in all three locations with the maximum abundance in the Cholistan Institute of Desert Studies. Megachile cephalotes remained active from March to September (the spring and summer seasons). In a nest, females made 7-8 brood cells each having a length of 1.2-2.3 cm. Plant resin was used to construct cells and mud or animal dung to plug the nest entrance. A vestibular cell was also made between the outermost brood cell and the nest entrance that ranged from 1.4 to 2.5 cm in length. No intercalary cells were observed in the nests. The males took 65.3 days to become adults, while the females took 74.78 days. The sex ratio was significantly biased toward females in all three locations. Grewia asiatica was the predominant pollen grain species found in the brood cells. Megachile cephalotes were observed collecting resin from Acacia nilotica, Prosopis juliflora, and Moringa oleifera. Three cleptoparasites of this species were also recorded: Euaspis carbonaria, Coelioxys sp., and Anthrax sp. This study set up a background to encourage new studies on artificial nesting and provides tools for proper biodiversity management and conservation.

Phytochemical Profile, Plant Precursors and Some Properties of Georgian Propolis.

Propolis (bee glue) is a resinous substance produced by different species of bees i.a. from available plant resins, balsams, and exudates. It is characterized by significant biological activity (e.g., antimicrobial and antioxidant) and phytochemical diversity related to the available plant sources in specific geographical regions. The available scientific literature on propolis is quite extensive; however, there are only a few reports about propolis originating from Georgia. Therefore, our research was focused on the characterization of Georgian propolis in terms of phytochemical composition and antimicrobial/antioxidant activity. Performed research included UHPLC-DAD-MS/MS phytochemical profiling, determination of total phenolic and flavonoid content, antiradical and antioxidant activity (DPPH and FRAP assays) as well as antibacterial activity of propolis extracts obtained using 70% ethanol (70EE). Georgian propolis extracts exhibited strong activity against Gram-positive bacteria (22 mm-disc assay/64 µg/mL-MIC for S. aureus, sample from Imereti) and weaker against Gram-negative strains as well as strong antioxidant properties (up to 117.71 ± 1.04 mgGAE/g in DPPH assay, up to 16.83 ± 1.02 mmol Fe2+/g in FRAP assay for samples from Orgora and Qvakhreli, respectively). The phytochemical profile of Georgian propolis was characterized by the presence of flavonoids, free phenolic acids, and their esters. In most of the samples, flavonoids were the main chemical group (52 compounds), represented mainly by 3-O-pinobanksin acetate, pinocembrin, chrysin, galangin, and pinobanksin. The primary plant precursor of the Georgian bee glue is black poplar (Populus nigra L.) while the secondary is aspen poplar (P. tremula L.).

Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis.

Skin wound healing is a complex process that requires the mutual work of cellular and molecular agents to promote tissue restoration. In order to improve such a process, especially in cases of impaired healing (e.g., diabetic ulcer, chronic wounds), there is a search for substances with healing properties and low toxicity: two features that some natural products-such as the bee product named propolis-exhibit. Propolis is a resinous substance obtained from plant resins and exudates with antioxidant, anti-inflammatory, and antitumoral activities, among other biological ones. Based on the previously reported healing actions of different types of propolis, the Brazilian red propolis (BRP) was tested for this matter. A skin wound excision model in male Wistar rats was performed using two topical formulations with 1% red propolis as treatments: hydroalcoholic extract and Paste. Macroscopical, histological and immunohistochemical analysis were performed, revealing that red propolis enhanced wound contraction, epithelialization, reduced crust formation, and modulated the distribution of healing associated factors, mainly collagen I, collagen III, MMP-9, TGF-β3 and VEGF. Biochemical analysis with the antioxidants SOD, MPO, GSH and GR showed that propolis acts similarly to the positive control, collagenase, increasing these molecules' activity. These results suggest that BRP promotes enhanced wound healing by modulating growth factors and antioxidant molecules related to cutaneous wound healing.

European gas prices shut down resin plant

European gas prices shut down resin plant