Plant Quality Research Articles

As(III)-oxidizing and plant growth-promoting bacteria increase the starch biosynthesis-related enzyme activity, 2-AP levels, and grain quality of arsenic-stressed rice plants

BackgroundGrain quality is an important index of rice production, particularly when plants are grown under stress. Arsenic (As) contamination in paddy fields severely affects rice grain yield and quality. Here, the effects of As and combinations of As(III)-oxidizing bacteria (Pseudomonas stutzeri 4.25, 4.27, and 4.44) and plant growth-promoting bacteria (Delftia acidovorans KKU2500-12 and Cupriavidus taiwanensis KKU2500-3) on enzymes related to starch accumulation in grains and the grain quality of Khao Dawk Mali 105 rice cultivated in As-contaminated soil under greenhouse conditions were investigated.ResultsArsenic affected the activities of starch biosynthesis-related enzymes, and decreases of up to 76.27%, 71.53%, 49.74%, 73.39%, and 47.46% in AGPase, SSS, GBSS, SBE, and SDBE activities, respectively, and 9.42–61.07% in starch accumulation in grains were detected after growth in As-contaminated soil. However, the KKU2500-3/4.25 and KKU2500-3/4.44 combinations yielded the greatest enzyme activities in grains, and compared with the results observed in uninoculated seedlings, increases in starch accumulation of up to 51.16% and 23.81% were found in the inoculated seedlings after growth in medium- and high-As-contaminated soils, at 10–17 and 10–24 days after anthesis, respectively. The bacteria increased the 2-AP content in rice under As stress, possibly via the induction of proline, a 2-AP substrate. Bacterium-inoculated rice had significantly greater 2-AP levels than uninoculated rice, and 2.16–9.93% and 26.57–42.04% increases were detected in rice plants grown in medium- and high-As-contaminated soils, respectively.ConclusionsArsenic toxicity can be mitigated in rice growing under greenhouse conditions by maintaining starch biosynthesis, accumulating amylose, and increasing 2-AP content. The effectiveness of these bacteria should be validated in paddy fields; hence, safe rice grains with a good starch content and aroma could be produced.

Agro-technological aspects of growing branched annual apple nursery plants

The article presents an analytical review of Russian publications reporting the results of studies that search for optimal ways to affect apple plants in order to obtain branched annual plants. Since it has been proved that nursery plants of a certain age and quality are preferable for each type of intensive planting, as their use promotes the early maturity of trees thus ensuring a quick economic return on capital investments, it is currently relevant to study the production of apple tree planting material with specified biometric parameters. The use of mechanical impact on the above-ground part of grafted plants to suppress apical dominance is considered; the effectiveness of stimulating premature shoots in the crown area is confirmed. Much attention is paid by researchers to the chemical treatments of plants in the fields of fruit tree nurseries to improve the quality of annual plants. The use of cytokinin preparations and gibberellic acids is shown to be highly effective. The maximum effect on obtaining branched annual plants in most works was achieved in pomological apple varieties that are prone to the formation of premature shoots, with the budding on vegetatively propagated rootstocks of a first-grade commercial variety at a height of over 15 cm above the soil surface. One of the alternative ways of obtaining branched annual plants is wood grafting in spring and cultivation both in the field and in protected ground with a root-ball system. According to most researchers, the greatest effectiveness of the combined use of various techniques can be achieved only with strict adherence to conventional technologies for each method of cultivation.

Research progress on differentiation and regulation of plant chromoplasts.

Carotenoids, natural tetraterpenoids found abundantly in plants, contribute to the diverse colors of plant non-photosynthetic tissues and provide fragrance through their cleavage products, which also play crucial roles in plant growth and development. Understanding the synthesis, degradation, and storage pathways of carotenoids and identifying regulatory factors represents a significant strategy for enhancing plant quality. Chromoplasts serve as the primary plastids responsible for carotenoid accumulation, and their differentiation is linked to the levels of carotenoids, rendering them a subject of substantial research interest. The differentiation of chromoplasts involves alterations in plastid structure and protein import machinery. Additionally, this process is influenced by factors such as the ORANGE (OR) gene, Clp proteases, xanthophyll esterification, and environmental factors. This review shows the relationship between chromoplast and carotenoid accumulation by presenting recent advances in chromoplast structure, the differentiation process, and key regulatory factors, which can also provide a reference for rational exploitation of chromoplasts to enhance plant quality.

Dry Matter Accumulation, Water Productivity and Quality of Potato in Response to Regulated Deficit Irrigation in a Desert Oasis Region.

As one of the most important food crops, the potato is widely planted in the oasis agricultural region of Northwest China. To ascertain the impact of regulated deficit irrigation (RDI) on various facets including dry matter accumulation, tuber yield, quality and water use efficiency (WUE) of potato plants, a two-growth season field experiment under mulched drip irrigation was conducted in the desert oasis region of Northwest China. Water deficits, applied at the seedling, tuber formation, tuber expansion and starch accumulation stages, encompassed two distinctive levels: mild (55-65% of field capacity, FC) and moderate (45-55% FC) deficit, with full irrigation (65-75% FC) throughout the growing season as the control (CK). The results showed that water deficit significantly reduced (p < 0.05) above-ground dry matter, water consumption and tuber yield compared to CK, and the reduction increased with the increasing water deficit. A mild water deficit at the tuber formation stage, without significantly reducing (p > 0.05) yield, could significantly increase WUE and irrigation water use efficiency (IWUE), with two-year average increases of 25.55% and 32.33%, respectively, compared to CK. Water deficit at the tuber formation stage increased starch content, whereas water deficit at tuber expansion stage significantly reduced starch, protein and reducing sugar content. Additionally, a comprehensive evaluation showed that a mild water deficit at the tuber formation stage is the optimal RDI strategy for potato production, providing a good balance between yield, quality and WUE. The results of this study can provide theoretical support for efficient and sustainable potato production in the desert oasis regions of Northwest China.

Selecting appropriate forage cover crops to improve growth, yield, and fruit quality of wolfberry by regulation of photosynthesis and biotic stress resistance

Forage cover crops have the potential to improve soil quality and orchard productivity, but their effects on wolfberry (Lycium barbarum L.) plants are still unclear. In this study, we conducted field and greenhouse experiments between 2019 and 2021, with 10 forage species as cover crops. We observed the growth, yield, fruit quality, and photosynthetic characteristics of wolfberry plants, as well as the occurrence of plant diseases and pests. Based on averaged data for all forage species, cover cropping facilitated plant growth, maintained fruit yield, and promoted leaf photosynthesis in wolfberry compared to monocropping. This was exemplified by a notable increase in the branch number of wolfberry plants under ryegrass treatment, with marginal increase in branch length under evergreen grass (lvyuan 5) and mangold treatments. Cover cropping additionally improved wolfberry quality through increasing carotenoid, flavonoid, and ascorbic acid contents by 21%, 53%, and 127%, respectively (P< 0.05). The presence of mangold, alfalfa, sweet sorghum, ryegrass, and feather grass reduced powdery mildew-induced leaf damage in wolfberry plants by 69% (P< 0.05). When alfalfa, feather grass, and ryegrass were used, the risk of aphid infestation was lowered by 67% (P< 0.05). Collectively, the results indicated that mangold, ryegrass, and alfalfa were the optimal cover crops for sustainable wolfberry production in the study area. The use of appropriate forage cover crops enhanced plant growth and fruit quality of wolfberry by stimulating photosynthetic capacity and biotic stress resistance.

Application of microalgae in cauliflower fertilisation

In a circular bioeconomy, balancing inorganic fertilisers and microalgae supplementation is important for ensuring sustainable agriculture. This study aimed to evaluate the fertilising effects of live microalgae (mostly Chlorella vulgaris and a mixture of Chlorophyceae and Trebouxiophyceae) on cauliflower (Brassica oleracea L. subsp. botrytis) plant morphology, yield, and quality of cauliflower heads. Microalgae fertilisation effects were analysed by employing an experimental factorial design with two factors: inorganic fertiliser and microalgae, each at high and low dosage levels, and the results revealed higher plant height, wider stem diameter, and lower stem slenderness in high-dose combinations than those in other combinations. In addition, an increase in nitrate, calcium, potassium, and sodium content was observed in the sap of cauliflower leaves in high-dose combinations compared to those in low-dose combinations. The high-dose combination also increased the yield, number of commercial heads, weight of the heads, and number of curds per head of cauliflower. Overall, the study demonstrates that increasing the dose of inorganic fertilisers and microalgae significantly increases nutrient concentration in the sap, which translates into a larger plant, an increase in the number of leaves and yield, and an improvement in the quality of cauliflower heads. These findings underscore the necessity for further research to ascertain the optimal combination of inorganic fertilisers and microalgae to maximise crop yield and cauliflower head quality.

Response of Aeroponically Cultivated Baby-Leaf Lettuce (Lactuca sativa L.) Plants with Different Zinc, Copper, Iodine, and Selenium Concentrations

Over two billion people worldwide suffer from micronutrient deficiencies. Biofortifying vegetables can enhance micronutrient intake through the diet. This study assessed the biofortification of indoor-grown baby-leaf lettuce using aeroponics. Four experiments, two each, were conducted by adding different concentrations of Zn (from 10 to 450 µM) or Cu (from 3 to 250 µM) into a nutrient solution. A fifth experiment was conducted by simultaneously adding to the nutrient solution the optimal concentration of I (5 µM) and Se (13 µM), chosen on the basis of previous works, and the optimal concentration of Zn (250 µM) and Cu (150 µM), chosen on the basis of the results obtained in the first four experiments. Leaf biomass, mineral concentrations, chlorophylls, carotenoids, phenols, flavonoids, nitrates, and antioxidant capacity were measured 21 days after transplanting. Higher concentrations of Cu, Zn, I, or Se in the nutrient solution led to an increase in their concentrations in lettuce leaves, without affecting the growth or leaf quality of lettuce plants. The simultaneous application of I with the other elements induced a higher accumulation in leaves compared to when I is applied alone. One hundred grams of lettuce leaves biofortified with Se, I, Cu, and Zn would provide the 6.1%, 35.3%, and 263.0% of Adequate Intake for Cu, Se, and I, respectively, and 4.5% of Population Reference Intake for Zn. Our results suggest that simultaneously biofortifying baby-leaf lettuce with these four minerals is a practical and convenient way to integrate these micronutrients into the diet without reducing the yield or quality of lettuce.

Isolation and functional analysis of R2R3-MYB transcription factor PlMYB42 associated with stem strength in herbaceous peony

Lodging resistance is a vital agronomic trait of plants, which affects plant architecture, yield and quality. Herbaceous peony (Paeonia lactiflora Pall.) is considered a high-end cut flowers due to its unique color, pattern, and aroma of flowers, but the traditional cultivated varieties of P. lactiflora have stem lodging. Although several regulatory factors participated in stem development have been identified in other plants, few involved in P. lactiflora have been reported. Here, PlMYB42, a transcription factor isolated from P. lactiflora, was found associated with stem strength and belonged to R2R3-MYB subfamily. PlMYB42 was highly expressed in stems, and showed a rise trend along with stem development. PlMYB42 protein was located in the nucleus and exhibited transcriptional activation activity. Silence of PlMYB42 in P. lactiflora suppressed lignin accumulation and weakened stem strength, while overexpression of PlMYB42 in tobacco promoted stem lignification and increased stem strength. Expression levels of lignin biosynthesis related genes were significantly decreased in PlMYB42 silence lines. These results indicated that PlMYB42 might promote stem strength through lignin accumulation. This work would reveal the genetic basis of lignin accumulation and stem development in P. lactiflora, and provide new insights into plant stem development.

Foliar-applied melatonin and titanium nanoparticles modulate cadmium (Cd) toxicity through minimizing Cd accumulation and optimizing physiological and biochemical properties in sage (Salvia officinalis L.).

Notwithstanding the fact that melatonin (MT) and titanium nanoparticles (Ti NPs) alone have been widely used recently to modulate cadmium (Cd) stress in plants, there is a gap in the comparative impacts of these materials on lowering Cd toxicity in sage plants. The objective of this study was to determine how foliar application of MT and Ti NPs affected the growth, Cd accumulation, photosynthesis, water content, lipid peroxidation, and essential oil (EO) quality and quantity of sage plants in Cd-contaminated soils. A factorial experiment was conducted using MT at 100 and 200μM and Ti NPs at 50 and 100mg L-1 topically, together with Cd toxicity at 10 and 20mg Cd kg-1 soil. The results showed that Cd toxicity decreased plant growth and enhanced lipid peroxidation. The Cd stress at 20mgkg-1 soil resulted in increases in Cd root (693%), Cd shoot (429%), electrolyte leakage (EL, 29%), malondialdehyde (MDA, 72%), shoot weight (31%), root weight (27%), chlorophyll (Chl) a + b (26%), relative water content (RWC, 23%), and EO yield (30%). The application of MT and Ti NPs controlled drought stress by reducing MDA and EL, enhancing plant weight, Chl, RWC, and EO production, and minimizing Cd accumulation in plant tissues. The predominant compounds in the EO were α-thujone, 1,8-cineole, β-thujone, camphor, and α-humulene. MT and Ti NPs caused α-thujone to rise, whereas Cd stress caused it to fall. Based on heat map analysis, MDA was the trait that was most sensitive to treatments. In summary, the research emphasizes the possibility of MT and Ti NPs, particularly MT at 200μM, to mitigate Cd toxicity in sage plants and enhance their biochemical reactions.

Topping and NPK fertilization alter seed germination, plant growth and active components of Valeriana amurensis.

Valeriana amurensis, a tall herbaceous plant, has been traditionally utilized as a herbal remedy both in China and Russia. This study was set to explore how to cultivate high quality seedlings, considering factors such as seedling time, seeding density, shading, and plastic film mulching. In addition, we investigated the impact of topping and fertilizer on the growth and quality of V. amurensis. According to the percentage of seed germination, the seeds of V. amurensis were sowed with 0.88 g m-2 density using plastic film mulching from late April to mid-May (germination percentage was more than 30%). The optimal Transplanting time was identified as late September, ensuring a high survival rate of 98%. Generally, topping showed the most improved growth indices in late fruit period (21.87 for number of radical leaves) and withering period (6.50 for number of buds and 234.81 for number of roots). Topping increased the yields of valtrate (10.91 mg per plant), valepotriates (809.51 mg per plant) and essential oil (395.64 mg per plant) in withering period. Nitrogen fertilizer promoted maximum root growth and increased the biomass of V. amurensis. Meanwhile, N fertilizer significantly increased the yields of valtrate to 10.46 mg per plant and valepotriates to 772.32 mg per plant among three types of fertilizers. Seedlings are obtained according to rational sowing factors and transplanting time. Topping and nitrogen fertilization emerge as superior strategies to enhance the growth and medicinal quality of this valuable plant. This study provides actionable insights for the cultivation V. amurensis.

Zno nanoparticles: improving photosynthesis, shoot development, and phyllosphere microbiome composition in tea plants

BackgroundNanotechnology holds revolutionary potential in the field of agriculture, with zinc oxide nanoparticles (ZnO NPs) demonstrating advantages in promoting crop growth. Enhanced photosynthetic efficiency is closely linked to improved vigor and superior quality in tea plants, complemented by the beneficial role of phyllosphere microorganisms in maintaining plant health. However, the effects of ZnO NPs on the photosynthesis of tea plants, the sprouting of new shoots, and the community of phyllosphere microorganisms have not been fully investigated.ResultsThis study investigated the photosynthetic physiological parameters of tea plants under the influence of ZnO NPs, the content of key photosynthetic enzymes such as RubisCO, chlorophyll content, chlorophyll fluorescence parameters, transcriptomic and extensive targeted metabolomic profiles of leaves and new shoots, mineral element composition in these tissues, and the epiphytic and endophytic microbial communities within the phyllosphere. The results indicated that ZnO NPs could enhance the photosynthesis of tea plants, upregulate the expression of some genes related to photosynthesis, increase the accumulation of photosynthetic products, promote the development of new shoots, and alter the content of various mineral elements in the leaves and new shoots of tea plants. Furthermore, the application of ZnO NPs was observed to favorably influence the microbial community structure within the phyllosphere of tea plants. This shift in microbial community dynamics suggests a potential for ZnO NPs to contribute to plant health and productivity by modulating the phyllosphere microbiome.ConclusionThis study demonstrates that ZnO NPs have a positive impact on the photosynthesis of tea plants, the sprouting of new shoots, and the community of phyllosphere microorganisms, which can improve the growth condition of tea plants. These findings provide new scientific evidence for the application of ZnO NPs in sustainable agricultural development and contribute to advancing research in nanobiotechnology aimed at enhancing crop yield and quality.Graphical

Field evaluation of biostimulants on growth, flowering, yield, and quality of snap beans in subtropical environment

The cultivation of snap beans (Phaseolus vulgaris L.) in subtropical regions faces environmental challenges leading to potential declines in yield. This study explores the efficacy of biostimulants as a solution, specifically investigating spraying treatments with 6-benzylaminopurine (6-BA), chitosan (Ch), triacontanol (TRIA), and potassium silicate (KSi) on the snap bean cv. Paulista. Over two growing seasons with late sowing and elevated summer temperatures, the research assesses growth, flowering, yield, and quality. Notably, 5 ppm TRIA demonstrates the most significant impact on plant growth and leaf nutrient content. Treatments with 40 ppm 6-BA, 5 ppm TRIA, or 200 ppm KSi exhibit notable effects on inflorescence flower count and flowers per plant. These treatments prove most effective for crucial green pod yield measures, including the number and weight of marketable pods. Moreover, 40 ppm 6-BA or 5 ppm TRIA significantly enhances pod characteristics, such as length, diameter, and weight, consistently improving over both seasons. Particularly, 5 ppm TRIA outperforms in enhancing the chemical quality of pods throughout the study. Overall, the findings suggest that the application of 5 ppm TRIA offers the most favorable enhancements for the growth, flowering, productivity, and quality of snap bean plants in subtropical field conditions.

Temporal change and impact on air quality of an energy recovery plant using the M-BACI design in Gipuzkoa

A significant concern in our society is the potential impact on both health and the environment of air pollutants released during the incineration of waste. Therefore, it is crucial to conduct thorough control and monitoring measures. In this context, the objective of this research was to study the evolution of particulate matter (PM2.5) and associated trace elements during the period before and after the installation of an Energy Recovery Plant (ERP). For that, a descriptive and temporal analysis of PM2.5 concentration and composition were performed on two similar areas (impact/control) using the Before-After/Control-Impact (BACI) design and two periods (before from January 01, 2018 to February 06, 2020 and after from December 10, 2020 to September 30, 2022). Results showed a decrease in the levels of PM2.5 and associated trace elements is observed in the impact zone (IZ) and in the control zone (CZ) throughout the study period. In the case of PM2.5, the most notable decrease occurred in the period of the start-up of the ERP, a period that coincides with the confinement and restrictions of COVID, with a subsequent increase in both zones, without reaching the levels observed in the period prior to the start-up of the ERP. Selenium is the only trace element that increases significantly in the IZ. In conclusion, a decrease is observed for all pollutants except selenium in both zones, although less pronounced in the IZ. Since selenium already showed an upward trend in the phase prior to the start of the ERP, it is necessary to investigate its evolution and find out the possible cause.

SlPGR5/SlPGRL1 pathway-dependent cyclic electron transport regulates photoprotection and chloroplast quality in tomato plants

The essential photoprotective role of proton gradient regulation 5 (PGR5)-dependent cyclic electron flow (CEF) has been reported in Arabidopsis, rice, and algae. However, its functional assessment has not been performed in tomato yet. In this study, we focused on elucidate the function of SlPGR5 and SlPGR5-like photosynthetic phenotype 1 (PGRL1) in tomato. We performed RNA interference and found that SlPGR5/SlPGRL1-suppressed transformants exhibited extremely low CO2 assimilation capacity, their photosystem I (PSI) and PSII were severely photoinhibited and chloroplasts were obviously damaged. The SlPGR5/SlPGRL1-suppressed plants almost completely inhibited CEF and Y(ND), and PSII photoinhibition may be directly related to the inability to produce sufficient proton motive force to induce NPQ. The transgenic plants overexpressing SlPGR5 and SlPGRL1 driven by 35S promoter capable alleviate photoinhibition of plants under low night temperature. The transcriptomic and proteomic analyses suggested that the nuclear gene transcription and turnover of chloroplast proteins, including the plastoglobule-related proteins, were closely related to SlPGR5/SlPGRL1 pathway dependent CEF. The bridge relationship between CEF and chloroplast quality maintenance was a novel report to our knowledge. In conclusion, these results revealed the regulatory mechanism of the SlPGR5/SlPGRL1 pathway in photoprotection and maintenance of chloroplast function in tomato, which is crucial for reduce yield loss, especially under adverse environmental conditions.

Biocontrol potential of plant growth-promoting rhizobacteria against plant disease and insect pest.

Biological control is a promising approach to enhance pathogen and pest control to ensure high productivity in cash crop production. Therefore, PGPR biofertilizers are very suitable for application in the cultivation of tea plants (Camellia sinensis) and tobacco, but it is rarely reported so far. In this study, production of a consortium of three strains of PGPR were applied to tobacco and tea plants. The results demonstrated that plants treated with PGPR exhibited enhanced resistance against the bacterial pathogen Pseudomonas syringae (PstDC3000). The significant effect in improving the plant's ability to resist pathogen invasion was verified through measurements of oxygen activity, bacterial colony counts, and expression levels of resistance-related genes (NPR1, PR1, JAZ1, POD etc.). Moreover, the application of PGPR in the tea plantation showed significantly reduced population occurrences of tea green leafhoppers (Empoasca onukii Matsuda), tea thrips (Thysanoptera:Thripidae), Aleurocanthus spiniferus (Quaintanca) and alleviated anthracnose disease in tea seedlings. Therefore, PGPR biofertilizers may serve as a viable biological control method to improve tobacco and tea plant yield and quality. Our findings revealed part of the mechanism by which PGPR helped improve plant biostresses resistance, enabling better application in agricultural production.

Smart irrigation system using node microcontroller unit ESP8266 and Ubidots cloud platform

The agricultural irrigation system is extremely important. For optimal harvest yields, farmers must manage rice plant quality by monitoring water, soil, and temperature on agricultural fields. If market demand rises, traditional rice field irrigation in Indonesia will make things harder for farmers. This modern era requires a system that lets farmers monitor and regulate agricultural fields anywhere, anytime. We need a solution that can control the irrigation system remotely using an internet of things (IoT) device and a smartphone. This study employed the Ubidots IoT cloud platform. In addition, the study uses soil moisture and temperature sensors to monitor conditions in agricultural regions, while pumps function as irrigation systems. The test results indicate the proper design of the system. Each trial collected data. The pump will turn on and off automatically based on soil moisture criteria, with the pump active while the soil moisture is less than 20% and deactivated when the soil moisture exceeds 20%. In simulation mode, the pump operates for an average of 0–5 seconds of watering. The monitoring system shows the current soil temperature and moisture levels. Temperature sensors respond in 1-3 seconds, whereas soil moisture sensors respond in 0–4 seconds.

Smart irrigation system using node microcontroller unit ESP8266 and Ubidots cloud platform

The agricultural irrigation system is extremely important. For optimal harvest yields, farmers must manage rice plant quality by monitoring water, soil, and temperature on agricultural fields. If market demand rises, traditional rice field irrigation in Indonesia will make things harder for farmers. This modern era requires a system that lets farmers monitor and regulate agricultural fields anywhere, anytime. We need a solution that can control the irrigation system remotely using an internet of things (IoT) device and a smartphone. This study employed the Ubidots IoT cloud platform. In addition, the study uses soil moisture and temperature sensors to monitor conditions in agricultural regions, while pumps function as irrigation systems. The test results indicate the proper design of the system. Each trial collected data. The pump will turn on and off automatically based on soil moisture criteria, with the pump active while the soil moisture is less than 20% and deactivated when the soil moisture exceeds 20%. In simulation mode, the pump operates for an average of 0–5 seconds of watering. The monitoring system shows the current soil temperature and moisture levels. Temperature sensors respond in 1-3 seconds, whereas soil moisture sensors respond in 0–4 seconds.

Evaluating temperature variation of nutrient solution in Nutrient Film Technique (NFT) cooling system for temperate vegetable

The production of leafy green vegetables in hydroponic systems within greenhouses has gained global prominence in the fresh vegetable production industry. Hydroponic systems, particularly Nutrient Film Technique (NFT), are highly effective due to their soilless nature and efficient nutrient and water circulation. However, maintaining ideal greenhouse temperatures can be challenging. One strategy to reduce energy costs is to heat or cool the nutrient solution instead of the entire greenhouse environment. By regulating the root zone temperature through nutrient solution management, growers can provide more favourable conditions for temperate leafy greens. These conditions are critical because temperatures above 23°C can inhibit plant growth, while temperatures exceeding 35°C can significantly reduce plant metabolism efficiency. This study investigates how lettuce responds to nutrient solution cooling. Importantly, this research focuses on Malaysia, an equatorial country with a consistently warm and humid climate. In such environments, controlling nutrient solution or air temperature to match the crops' optimal growth requirements is crucial. The study employed Butterhead and Romaine lettuce varieties in a hydroponic NFT system. The EC (Environmental Cooling) treatment involved cooling the nutrient solution, while the RZC (Root Zone Cooling) treatment utilized environmental cooling. The results demonstrated that maintaining a consistent nutrient solution temperature within the range of 14°C to 18°C (as observed in the EC treatment) led to superior plant growth and quality, particularly in the early growth stages. In contrast, the RZC treatment showed temperature fluctuations, causing plant stress and inhibiting growth.

Effect of Organic and Inorganic Fertilizers on Growth, Yield and Quality of Okra (Abelmoschus esculentus L. Moench)

The study, conducted at the Agricultural Research Farm, School of Agriculture, S.G.V.U., Jaipur (Rajasthan) during the Rabi season of 2022-23, examined the impact of organic and inorganic fertilizers on the growth, yield, and quality of Okra (Abelmoschus esculentus L. Moench). Using a Randomized Block Design (RBD) with 13 treatment combinations, including control and various mixes of fertilizers with organic amendments like farmyard manure, vermicompost, poultry manure, neem cake, and biofertilizers, the research found that treatment T5 (75% RDF + 25% N through Vermicompost) had a notable impact on vegetative development, yield, and quality indices, also showing the highest benefit-to-cost ratio of 2.26 compared to the control group's 1.14. The combined application of organic and inorganic fertilizers significantly improved Okra's growth, yield, and quality, with T5 demonstrating the best performance in plant height, fruit yield, and quality traits like ascorbic acid and sugar content, highlighting its potential for sustainable and profitable okra production and the benefits of integrating organic manures with inorganic fertilizers for enhancing crop productivity and soil health.

The Effects of Frass and Vermicompost Fertilization on the Biometrical Parameters of Plant and Soil Quality, and the Rhizobiome, in Red Beet (Beta vulgaris L.) Cultivation

Insect frass and vermicompost hold potential applications as fertilizers, with their abilities to improve plant resilience against unfavorable environmental conditions and increase their resistance to pests and diseases. In this study, we explored the effects of vermicompost fertilization, mealworm frass, and superworm frass as potential plant fertilizers for red beet cultivation. We analyzed the connections among chemical parameters, rhizobiome structure and function, and the biometrics of fertilizer-treated plants. In general, soils enriched with vermicompost and superworm frass exhibited the highest macroelement contents. Dry superworm frass fertilization was characterized by the increased availabilities of total nitrogen, NH4-N, and NO3-N. The use of vermicompost and mealworm frass resulted in significantly higher red beet biomass values. The presence of the highest N-fixation potential and key hormonal substances involved in plant development, such as auxins and gibberellins, was demonstrated using wet superworm frass. The results indicated that wet superworm frass, similar to vermicompost and dry superworm frass, exhibits high chemoheterotrophic potential. This suggests an r-type strategy and high adaptive flexibility of rhizobial bacteria. As a consequence, both life in the root zone and the microbiome itself may be better adapted to sudden, unfavorable environmental changes or attacks by plant pathogens.