Nurserymen Research Articles

Intensively Cultivated Roman Villae Estates: Case Study of Medulin Bay (Istria, Croatia)

ABSTRACTOur image of the Roman landscape of Istria is characterised by large‐scale centuriation and architectural remains of Roman villae. Detection and mapping of other, less dramatic landscape features require systematic large‐scale prospection, but this faces significant difficulties in the Mediterranean environment. However, the developments in the field of airborne laser scanning offer the possibility to create archaeologically usable digital terrain models under water and under very dense and low maquis vegetation. This paper reports on the use of terrain models created using a green laser and a sophisticated archaeologically driven ground point filtering strategy. Combined with archaeological aerial photo interpretation, this provides the means for landscape mapping and interpretation that has revealed a wealth of archaeological structures hinting at Roman agricultural practices and landscape. Our case study is based on a laser scan of about 24 km2 of land and underwater terrain in Medulin Bay. Processed, visualised and interpreted for archaeological purposes, the data reveal not only features ranging from prehistoric hilltop settlements to modern military installations but also features a complex picture of the Roman land use. Of particular interest is the large number of planting pits, which extend over a total length of 4 km. They were laid on a regular grid of approximately 35 × 35 m, sometimes combined in contiguous parcels. They can be interpreted as remains of orchards or tree nurseries of Roman date, and the paper examines also the question of whether they can be linked to the associated Roman estates. The case study area presented here shows that the potential of remote sensing methods goes far beyond the mere finding of traditional sites but can open up new landscape‐scale perspectives on regions that have been archaeologically little explored.

Evaulation of Effective Antagonistic Yeasts in Kalasin Province, a Biocontrol Agent against the Fungal Colletotrichum capsici Caused Anthracnose Diseases in Chili Fruits

Primary screening of epiphytic and endophytic yeasts, the majority yeast was found from rhizosphere soil (21 isolates, 31.81%), and 5 antagonistic yeasts can have inhibited the growth of mycelia of C. capsici up to 60%. Efficacy of five antagonistic yeasts were screened under plant nurseries conditions. The result found all isolates were tested and compared with control and chemical plots. The result of plant height showed that, yeast isolate KS34 had highest of plant height at 45.80 cm. For the result of branch per plant of chili plant found isolate KS37 showed highest of branch per plant at 16.60 branches and the flower of chili per plant presented that isolate KS37 had highest of flower at 18.60 flowers. The result of anthracnose disease control by antagonistic yeasts in disease incidence and category mean as plant resistance. These results found that, Isolates KS46 had a great disease incidence at 7.80% that mean this isolate prove chili plant to resistance to C. capsisci. Addition, isolate KS46 had highest of activity of chitinase enzyme at 170.72 U/ml. Isolate KS46 identified by D1/D2 domain of 26S ribosomal DNA sequencing. The sequence analysis showed maximum identity of 100% with Candida haemulonii. This assumed C. haemulonii was showed the best to control C. capsici. For confirmed this result, all isolates were determined the mode of action by studied in relationship between antagonist isolates. The action of yeast isolate could competition the nutrient with mycelia of pathogen. This mode effect to C. capsici loss nutrient caused the pathogen get stuck to braked and died later.

Community Tree Nurseries in the UK: Promise, Potential and Pitfalls

Forest restoration and tree-planting projects initiated as a response to climate change and biodiversity loss are increasingly important around the globe. Small-scale and community-based tree nurseries have been promoted as a potential route to meeting some local or regional supply-side bottlenecks. A study in the UK used a mixed methods approach to assess the potential contribution of community tree nurseries (CTNs) to tree material supply. Semi-structured interviews (SSIs) were undertaken with 16 CTNs across the UK to generate a sector-wide characterisation of CTNs. A UK-wide online survey assessed the total number of CTNs, production methods and volumes, biosecurity practices and benefits of community involvement. Another 13 CTNs receiving support to establish and extend their operations took part in SSIs assessing their development. The results indicate that there are four broad types of CTN (Organisation- and project-based, Community-based, Enterprise and Network CTNs). A significant number of CTNs in Britain are new and establishing. The sustainability of CTNs relies in large part on grant support rather than income from tree sales. Production is almost exclusively native broadleaves and local provenances. There are policy implications concerning: i. the suitability of species being produced to future climatic and market conditions and ii. the tension around financial viability, grant dependence and CTN sustainability for those CTNs focused on social and environmental benefits rather than income generation. Identifying gaps in the provision of trees by commercial nurseries, e.g., uncommon or recalcitrant species, could be a feasible financial strategy. It is likely that financial support and skills development are likely to be required as social innovation in the community-supported tree nursery sector establishes and develops. Judging the success and impact of some types of CTNs in terms of production and finance measures may be unfair, as other social and environmental benefits may be as important and valuable. It is clear that more evaluative research is needed to quantify and better understand these kinds of outcomes and the added value they accrue.

Irrigation and Fertilization: A Comprehensive Analysis of Their Influences on Qualitative Indices in Two Plum Varieties

The chief aim of this study is to provide information regarding the value and effectiveness of localized irrigation applied to plum trees grown in nurseries; this study also emphasizes how irrigation impacts various qualitative indices in the context of different fertilization treatments. By increasing production in the nursery, the application of differentiated rules for fertilization and irrigation is expected to yield vigorous, healthy fruit tree planting material. As in the case of all cultivated plants, fruit trees in nurseries are primarily dependent on soil and climatic conditions. This research was carried out in a private fruit tree nursery in the northwestern part of Romania. The soil taxonomic unit identified on the research field was arable, weakly glaciated loamy clay on fluvial deposits. The two plum cultivars that were studied were Stanley and Cacanska Lepotica, both of which are valued for the high caliber of their fruit. This research was conducted using a 4 × 2 × 4 trifactorial experiment, with irrigation acting as the primary factor, cultivar as the secondary factor, and fertilization as the tertiary factor. During this research, the fertilization treatments proved to have the most significant impact (34.50%) on stem diameter compared with irrigation (20.67%) and cultivar (5.63%), given that the cultivar had no discernible influence on the increase in the diameters of the grafted trees.

Life cycle assessment of citrus tree nurseries in Uruguay: Are their environmental impacts relevant?

Perennial fruit production at the commercial scale, such as citrus fruits, begins with seedling production in a nursery. This stage lasts several months and involves different phases and the use of substrates and infrastructure. As the seedling does not produce fruit but does consume inputs, studying the environmental impacts associated with this stage becomes relevant, especially to understand its contribution to the total impact of the crop cycle. Despite the global relevance of fruit tree seedlings production, LCA studies in the literature focus on horticultural crop nurseries, and those on perennial tree nurseries do not consider both substrate and structures in the analysis, which is key for this type of crop since the main production system is soilless production in greenhouses. Thus, the main goal of this study is to quantify the environmental impacts related to the production of citrus fruit tree seedlings using LCA, analyse the main production system applied nowadays, and study its relevance with respect to the crop cycle. To this end, a certified Uruguayan citrus nursery was analysed, from which primary data was obtained. As well, methodological issues concerning water consumption and modelling emissions from input applications in soilless greenhouse systems are tackled. Results show that the main hotspots of the nursery stage are infrastructure production and peat transportation, which highlights the relevance of their inclusion when modelling the system. Extending the lifespan of the galvanised steel structures and decreasing substrate transport distances are shown to be effective measures to reduce environmental impacts. The contribution of the nursery stage to the citrus production cycle is negligible for almost all the impact categories assessed except cancer human toxicity, as it accounts for 0–3.6% of the impacts depending on the impact category. Great differences (from 10 to 400 times higher results on average) are observed when comparing the results with those from commercial databases, as they consider open-field nurseries where seedlings are grown in the soil. The need to develop harmonised methods to model water consumption and fertiliser and pesticide emissions for soilless crops in greenhouses arises. The present study presents a complete quantification of the environmental impacts of the main production system of citrus fruit tree seedlings and provides scientific and quantitative evidence of its contribution to the production cycle, helping decision-makers understand where efforts should be focused to achieve a more sustainable fruticulture.

The effect of ectomycorrhizal fungal exposure on nursery-raised Pinus sylvestris seedlings: plant transpiration under short-term drought, root morphology and plant biomass.

Drought is a major environmental stressor that limits seedling growth. Several studies have found that some ectomycorrhizal fungi may increase the drought tolerance of nursery-raised seedlings. However, the precise role that different ectomycorrhizal fungi species play in drought tolerance remains unclear. We evaluated the transpiration rate of Pinus sylvestris seedlings under drought stress in greenhouse conditions by exposing seedlings to 10 ectomycorrhizal fungi species, with different functional traits (exploration type and hydrophobicity), and to 3 natural soil inoculums. We measured the transpiration and water potential of the seedlings during a 10-day drought period and a 14-day recovery period. We then analyzed their root morphology, stem, needle, root biomass and needle chlorophyll fluorescence. We showed that exposing seedlings to ectomycorrhizal fungi or soil inoculum had a positive effect on their transpiration rate during the driest period and through the recovery phase, leading to 2- to 3-fold higher transpiration rates compared with the nonexposed control seedlings. Seedlings exposed to medium-distance ectomycorrhizal fungi performed better than other exploration types under drought conditions, but ectomycorrhizal fungi hydrophobicity did not seem to affect the seedlings response to drought. No significant differences were observed in biomass accumulation and root morphology between the seedlings exposed to different ectomycorrhizal fungi species and the control. Our results highlight the positive and species-specific effect of ectomycorrhizal fungi exposure on drought tolerance in nursery-raised Scots pine seedlings. The studied ectomycorrhizal fungi functional traits may not be sufficient to predict the seedling response to drought stress, thus physiological studies across multiple species are needed to draw the correct conclusion. Our findings have potential practical implications for enhancing seedling drought tolerance in nursery plant production.

Oxidative stress as markers in identification of aluminum-tolerant peach tree rootstock cultivars and clonal selections

ABSTRACT Peach rootstock and scion cultivars are selected in breeding programs considering resistance to pests and diseases, salt tolerance, drought tolerance, and vigor. However, rootstock tolerance to aluminum (Al), which is markedly present in tropical and subtropical soils of the world, is not considered. Thus, it is essential to define potential markers that can contribute to the selection of Al-resistant or Al-tolerant peach rootstocks. The objective of this study was to identify Al-tolerant peach tree rootstock cultivars and clonal selections using physiological and oxidative stress variables. A completely randomized experimental design was used in a 13 (rootstock cultivars and clonal selections) × 2 (with and without Al) factorial arrangement, with three replications. Nursery peach trees of own-rooted ‘BRS Mandinho’ (without rootstock) and nursery trees of ‘BRS Mandinho’ grafted on different rootstock cultivars and clonal selections were grown in a hydroponic system, consisting of two treatments, with and without 100 mg L -1 of Al. Dry biomass, photosynthetic variables, pigment concentration, hydrogen peroxide content, membrane lipid peroxidation, and activity of the antioxidant enzymes were evaluated. The total dry matter production of the own-rooted ‘BRS Mandinho’ trees and the SS-CHI-09-39 and SS-CHI-09-40 selections is affected by the Al presence, representing a decline of 35.4, 37.2, and 24.4 %, respectively, compared to the treatment with Al. The highest total dry matter production in Al presence was observed for the ‘Sharpe’ rootstock. ‘Capdeboscq’, DB-SEN-09-23, FB-ESM-09-43, JB-ESM-09-13, JAH-MAC-09-77, SAS-SAU-09-71, and VEH-GRA-09-55 rootstock selections are tolerant to Al. The use of physiological and biochemical variables shows potential for the selection of clonal rootstocks tolerant or resistant to Al.

Plant Infrastructure

This essay analyzes Miami as a place where plants are a major component of urban infrastructure. The centrality of tropical plants to the growth of Miami connects that city to the history of empire, where control of plant matter was the violent model for the standardization and distribution practices of modern infrastructure. In Miami, the US Department of Agriculture established a Plant Introduction Garden in 1898, with “Agricultural Explorer” David Fairchild activating networks connecting India, Kew Gardens, and Washington to bring mostly Asian tropical fruits, shrubs, and flowers to South Florida. A close reading of archives of botanical gardens, plant nurseries, and community organizations shows that Miami plant infrastructure was created jointly by these elite political/scientific networks and by vernacular, informal networks of Black labor migration and horticultural know-how. The essay focuses on the cultivation of Miami’s best-known plant product, the Haden mango, and the vital role in its propagation played by Black Bahamian gardener Nathan Sands, whose letters to his employer David Fairchild are preserved in the latter’s papers. Recovering the stories of people like Nathan Sands is vital to understanding the development of a global metropolis whose plant infrastructure erased the Black horticultural work on which it depended.

Genome‑Wide Identification and Expression Analysis of SPX Domain-Containing Gene Subfamily in Response to Phosphorus-Solubilizing Bacteria in Apple (Malus domestica)

Phosphate is one of the major elements that significantly affects fruit yield and quality. The aim of the study was to determine whether using phosphorus-solubilizing bacteria could produce high-quality apple nursery trees. Five different treatments were tested on a “Granny Smith” apple cultivar that was grafted onto an M.9 rootstock. These were 100% P, 50% P, 50% P + Bacillus megatarum (plant growth promoting rhizobacteria, PGPR), 0%P, and 0%P + PGPR. The study also identified the SPX gene family, which is essential for plant growth and development and responds to phosphorus (P) stress. A total of 72 SPX genes were identified in different plant species based on structural and phylogenetic analysis. The apple genome contains seven different SPX genes distributed on five of the 17 chromosomes. Gene structure and motif analysis showed that SPX genes show a relatively conserved exon/intron arrangement and motif composition in five different species: apple, strawberry, peach, apricot, and grape. Protein–protein network analysis showed that SPX proteins are closely related to proteins involved in P metabolism in apple. The digital expression profiles of MdSPX genes among 47 apple tissues were characterized to provide insight into their potential functions. RT-qPCR revealed that the expression level of all MdSPXs was significantly downregulated in 50% P + PGPR treatments, indicating that 50% P combined with PGPR is effectively taken up by the plant, saving it from Pi starvation. These results not only confirm the key role of MdSPXs in Pi homeostasis and the Pi signaling pathway but also clarify the importance of Pi-solubilizing bacteria in plant nutrition.

Practice and Biodiversity of Informal Ornamental Horticulture in Kinshasa, Democratic Republic of Congo

Biodiversity conservation is a key factor in meeting sustainable development goals. This is even more important in cities, where green spaces are becoming increasingly scarce. This study analyzes Kinshasa’s proliferating ornamental plant nurseries, known as informal horticultural sites (IHSs). The analysis focused on characterizing the profile of horticulturists, their production conditions, and the ornamental species produced. A total of 15 IHSs were sampled using the “snowball” technique, and 178 horticulturists were surveyed. Based on the socio-professional profile of the horticulturists, five groups of IHS are distinguished after a hierarchical clustering of principal components (HCPC). We found that IHSs exclusively employed men, most of whom were new to the trade, from all levels of education, and most of whom ranged from 19 to 45 years old. Production conditions are relatively similar from one site to another. However, all IHSs are characterized by permanent land insecurity, the use of phytosanitary products, plant-conditioning methods that are not very diversified and calibrated to growers’ investment capacities, and diversified seed acquisition methods. A total of 139 ornamental species, most of them exotic, were identified. Of these, 37% are phanerophytes, and 24% are considered potentially invasive. We suggest ways of professionalizing the activity and protecting the urban environment.

Individual nursery trees classification and segmentation using a point cloud-based neural network with dense connection pattern

Nurseries are used to cultivate a variety of tree species. Obtaining some specific information like the tree species, positions of crowns and trunks can enhance the efficacy of nursery management. Due to the robustness to illumination, the point cloud-based neural network models have become extensively employed in segmenting and classifying individual trees from large-scale data. However, few studies have focused on further processing the point clouds of individual trees. Therefore, D-PointNet++ (Dense PointNet++) is proposed in this paper for classifying tree species and segmenting different parts of trees (crowns, trunks, pots and supporting poles). D-PointNet++ utilizes a dense connection pattern in the feature extraction module, inspired by the architecture of DenseNet. Additionally, the proposed model uses a gating system and concatenation as fusion operations to combine point cloud features with different dimensions to improve accuracy. The point cloud data of seven different types of garden trees in the nursery was collected using a laser sensor. The experimental results demonstrate that D-PointNet++ surpasses two representative baseline methods, PointNet and PointNet++, in terms of both classification and segmentation accuracy. For the self-made nursery dataset, the classification overall accuracy (OA) and class accuracy (mAcc) of D-PointNet++ can reach 92.65% and 92.54%; the average Intersection over Union (mIoU) and mAcc can reach 89.90% and 92.18%, respectively. The proposed D-PointNet++ can provide more accurate information on each tree and is beneficial to the management of the nursery.

Beneficial Effect of Exogenously Applied Calcium Pyruvate in Alleviating Water Deficit in Sugarcane as Assessed by Chlorophyll a Fluorescence Technique

The growing demand for food production has led to an increase in agricultural areas, including many with low and irregular rainfall, stressing the importance of studies aimed at mitigating the harmful effects of water stress. From this perspective, the objective of this study was to evaluate calcium pyruvate as an attenuator of water deficit on chlorophyll a fluorescence of five sugarcane genotypes. The experiment was conducted in a plant nursery where three management strategies (E1—full irrigation, E2—water deficit with the application of 30 mM calcium pyruvate, and E3—water deficit without the application of calcium pyruvate) and five sugarcane genotypes (RB863129, RB92579, RB962962, RB021754, and RB041443) were tested, distributed in randomized blocks, in a 3 × 5 factorial design with three replications. There is dissimilarity in the fluorescence parameters and photosynthetic pigments of the RB863129 genotype in relation to those of the RB041443, RB96262, RB021754, and RB92579 genotypes. Foliar application of calcium pyruvate alleviates the effects of water deficit on the fluorescence parameters of chlorophyll a and photosynthetic pigments in sugarcane, without interaction with the genotypes. However, subsequent validation tests will be necessary to test and validate the adoption of this technology under field conditions.

A Survey of Phytophthora spp. in Eastern Indian Nurseries and Their Sensitivity to Six Oomycete-Targeted Commercial Fungicides.

A survey of the flori-horticultural nurseries in eastern India found Phytophthora nicotianae to be the most widespread Phytophthora species associated with different foliar symptoms of nursery plants and identified the presence of P. palmivora in eastern Indian nurseries for the first time. The survey also led to the first worldwide finding of P. nicotianae on Dipteracanthus prostratus (Poir.) Nees; Ocimum tenuiflorum L. (syn. Ocimum sanctum L.); Philodendron xanadu Croat, Mayo & J. Boos; and Pyrostegia venusta (Ker-Gawl.) Miers and P. palmivora on Episcia cupreata (Hook.) Hanst., as well as the first report from India of P. nicotianae on Spathiphyllum wallisii Regel; Anthurium andraeanum Linden ex André; and Adenium obesum (Forsk.) Roem. & Schult. Sensitivity to commercial fungicides Glazer 35WS, Rallis India (metalaxyl, FRAC code 4); Ridomil Gold, Syngenta (mefenoxam + mancozeb); Revus, Syngenta (mandipropamid, FRAC code 40); Aliette Bayer (fosetyl-Al, FRAC code 33); Acrobat, BASF (dimethomorph, FRAC code 40); and Amistar, Syngenta (azoxystrobin, FRAC code 11) was analyzed, showing EC50 values ranging from 0.75 to 16.39 ppm, 0.74 to 1.45 ppm, 2.43 to 17.21 ppm, 63.81 to 327.31 ppm, 8.88 to 174.69 ppm, and 0.1 to 1.13 ppm, respectively, with no cross-resistance of the isolates to the fungicides. The baseline information produced about these Phytophthora spp. from ornamental and horticultural host associations could help prevent the pathogens from becoming primary drivers of new disease outbreaks and their large-scale distribution beyond their natural endemic ranges.

Bacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors

Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation in the xylem of small and large citrus plants and the potential of the bacteria to cause ACP nymphs mortality that fed on shoots from Bt-treated citrus.

Soil solarization as a non-chemical weed control method in tree nursery production systems of the Pacific Northwest, USA

IntroductionHerbicide application in tree nurseries is limited because of the potential for chemical injury to the large diversity of trees species grown, the lack of registered products, and increasing restrictions on herbicide use, necessitating the costly practice of hand weeding. Soil solarization can reduce the weed seedbank by trapping solar energy under clear plastic film, resulting in high soil temperatures lethal to imbibed weed seeds and seedlings. The objective of this study was to determine if soil solarization would be an effective weed management strategy in Pacific Northwest, USA, tree production systems.MethodsField studies were conducted at three commercial tree nurseries in Oregon and Washington over two years to test soil solarization in reducing the naturally occurring weed seedbank and the time required to hand weed fields. Further field and laboratory tests were conducted with five weed species: Poa annua, Polygonum pensylvanicum, Amaranthus retroflexus, Portulaca oleracea, and Cyperus esculentus. Weed seeds and tubers were buried in packets at 5 and 10 cm to determine their viability after 6 weeks of solarization. A laboratory study was conducted with all but C. esculentus to quantify the exposure time at 45, 50, and 55°C required for 90% death (T90).ResultsSoil solarization was particularly effective in reducing the emergence of naturally occurring weeds in the fall and winter, when weed emergence was reduced by 94-96%. Emergence was reduced 67-81% during the subsequent spring and early summer. Nine to ten months after solarization, solarized areas had a 52 – 69% reduction in hand weeding time compared to non-solarized areas. In field trials with buried seed and tuber packets, mortality differed by location and depth, with P. annua and P. pensylvanicum having the greatest percent seed mortality followed by A. retroflexus and variable results for P. oleracea and C. esculentus. In lab studies, seed mortality differed depending on species and temperature; however, at 55°C, there was a relatively rapid drop in seed viability for all species, and T90 values ranged from 1.2 to 41 h whereas at 45°C the range was 47 to > 3000 h. Similar to the field studies, P. annua and P. pensylvancium were more sensitive to heat, followed by A. retroflexus and P. oleracea.ConclusionSoil solarization can be an effective weed management tool in reducing the weed seedbank in Pacific Northwest tree nurseries and other fall-sown crops but may not work for certain, thermotolerant weed species such as C. esculentus.

Differential accumulation of phenolics and phytoalexins in seven Malus genotypes cultivated in apple replant disease-affected soil

Apple replant disease (ARD) is a globally recognised soil-borne disease, which affects apple orchards and tree nurseries. Biotic factors are considered to be the main causal agent of ARD. Phenolics and phytoalexins were induced in apple roots grown in ARD soil compared to roots grown in disinfested ARD soil. However, the relationship between ARD susceptibility and production of phenolics and phytoalexins in apple roots is still unclear. To answer this question, we cultivated seven Malus genotypes with varying levels of ARD susceptibility (M.26, EMR 2, M.200, G.214, G.935, Selection 4, and MAL0739) in both ARD soil from Heidgraben, Germany, and the same soil after disinfestation by gamma-irradiation (γ-ARD). Despite the overall growth reduction observed for all seven genotypes, three of them, EMR 2, G.935 and MAL0739, showed better performance, as indicated by the ARD susceptibility index (ASI) and differences in their root structure. Phenolics and phytoalexins were differentially induced in the roots grown for four weeks (T4) and eight weeks (T8) in ARD soil compared to γ-ARD soil. Moreover, differences in the total phenolics content (TPC) and the total phytoalexin content among the evaluated Malus genotypes were observed. The biochemical data from the early time point (T4) separated Malus genotypes more effectively compared to that from the late time point (T8). Among the six ARD-induced phytoalexins, 3-hydroxy-5-methoxybiphenyl at T4 can serve as an early biomarker for ARD. Although TPC and total phytoalexin content of the Malus genotypes exhibited no or weak correlation with root and shoot growth reduction, some individual phenolics and phytoalexins showed higher coefficients, suggesting that they might influence the ARD susceptibility of apple rootstocks.

Polytomous key for selecting plants according to decorative, phenological, phenotypic traits when landscaping cities in the south of Russia

The classic selection of plants for landscaping the urban environment is a rather labor-intensive process involving the use of a large number of reference materials, atlases and plant catalogs. It is necessary to consider various characteristics of plants, taking into account their decorativeness, mutual compatibility, survival in the natural conditions of each region, etc. The purpose of the research was developing a polytomous key and creating it using a modern computer program to simplify the selection of plants according to decorative, phenotypic and phenological characteristics for landscaping cities in the southern Russia. The method of compiling a polytomous key was used as a way to identify plants by several leading characteristics, when the original groups were simultaneously divided into several smaller ones. The life forms, size, habitus of plants, characteristics of the trunk and crown, characteristics of flowering and fruiting, as well as environmental requirements were taken into account. On the basis of the research results the assortment of ornamental plants in large nurseries of the Krasnodar Territory and the Republic of Adygea, a list of plant names was compiled and their characteristics were encoded using a polytomous key. The key consisted of 8 sections, thanks to which it was possible to give the most complete description of the plant according to 36 parameters. The resulting database received state registration certificate No. 2023621905. The base and operation of the polytomous key are like a computer program. The functioning of the program has been successfully tested. The created program greatly simplifies the work of choosing an assortment of plants for landscaping, reducing it to a few minutes.

Addressing Ergonomic Challenges in Agriculture through AI-Enabled Posture Classification

In this study, we explored the application of Artificial Intelligence (AI) for posture detection in the context of ergonomics in the agricultural field. Leveraging computer vision and machine learning, we aim to overcome limitations in accuracy, robustness, and real-time application found in traditional approaches such as observation and direct measurement. We first collected field videos to capture real-world scenarios of workers in an outdoor plant nursery. Next, we labeled workers’ trunk postures into three distinct categories: neutral, slight forward bending and full forward bending. Then, through CNNs, transfer learning, and MoveNet, we investigated the effectiveness of different approaches in accurately classifying trunk postures. Specifically, MoveNet was utilized to extract key anatomical features, which were then fed into various classification algorithms including DT, SVM, RF and ANN. The best performance was obtained using MoveNet together with ANN (accuracy = 87.80%, precision = 87.46%, recall = 87.52%, and F1-score = 87.41%). The findings of this research contributed to the integration of computer vision techniques with ergonomic assessments especially in the outdoor field settings. The results highlighted the potential of correct posture classification systems to enhance health and safety prevention practices in the agricultural industry.

Host Status of Ornamental Shade Trees and Shrubs to Plant Parasitic Nematodes.

Oregon leads the United States in nursery production of shade trees and is third in deciduous and broadleaf evergreen shrub production. Plant-parasitic nematodes have been implicated in problems with the growth of plants in nurseries and are also of phytosanitary risk. A greenhouse experiment was conducted to evaluate the host status of four trees (Quercus alba, Quercus garryana, Acer campestre, Thuja occidentalis) and two shrubs (Buxus sempervirens, Rhododendron catawbiense) to Meloidogyne incognita, Meloidogyne hapla, and Pratylenchus neglectus. Each plant/nematode treatment was replicated five times, and the experiment was conducted twice. Plants were inoculated with 3,000 eggs of M. incognita or M. hapla and 2,500 individuals of P. neglectus two weeks after planting. After three months, the plants were harvested, and the total density of nematodes in soil and roots for P. neglectus and the total density of second-stage juveniles (J2) in soil and eggs on roots for M. hapla and M. incognita were determined. The final nematode population (Pf) and reproductive factor (RF = Pf/initial population density) were calculated. For M. incognita and M. hapla, all of the ornamental trees and shrubs would be considered as fair to good hosts with RF values > 1. Meloidogyne incognita had the highest Pf (5,234 total J2 and eggs/pot) and RF value (28.4) on A. campestre. For P. neglectus, all of the ornamental trees and shrubs were fair to good hosts, except for B. sempervirens. Buxus sermpervirens was not a host for P. neglectus, with an RF value of almost 0. This is the first report of Q. alba, Q. garryana, and A. campestre as hosts for M. incognita, M. hapla, and P. penetrans. This is also the first report of T. occidentalis and R. catawbiense as hosts for P. penetrans and the non-host status of B. sermpervirens for P. penetrans.

Community-based Agrotourism Development: The Analysis Strategy of Government of Bantaeng Regency, Indonesia

The development of agrotourism can not only encourage economic growth but also increase the positive perception of farmers and the public for preserving agricultural land as new business land. For this purpose, the government needs to take steps for preserving the environment, through means of agrotourism development in order to benefit the community. Through qualitative methods, this study aims to analyze the strategies and efforts that can be made by the government in developing communitybased agrotourism. Research data were obtained from observation, education literature, and interviews, before initiating the analysis, data triangulation process was conducted. The results of the analysis show that the government needs to develop space utilization so that people can develop new agrotourism business products, make promotional efforts to attract visitors, and provide new plant nursery facilities to the community. In addition, to maximize community-based agritourism in Bantaeng district, the government needs to provide training to increase human resources and outreach to the community.
 Keywords: agrotourism development, government, business land