Plant Fresh Weight Research Articles

Development of effective micropropagation protocols for endangered Gentiana lutea and Gentiana boissieri (endemic to Türkiye)

Abstract Gentiana spp. are ornamental as well as valuable medicinal plants. This research was conducted to investigate the effects of different cytokinin and auxin treatments on shoot propagation and rooting of both G. lutea and G. boissieri, and to establish the most appropriate micropropagation protocols for these species. For this purpose, MS media containing benzyl adenine (BA) (1, 2, 3, and 4 mg/l), zeatin (0.5, 1, 2, and 3 mg/l), and kinetin (0.5, 1, 2, and 3 mg/l) were used for shoot propagation, and the optimal cytokine applications were determined by examining shoot number per explant (SN/E) and shoot length (SL). To determine rooting ability, shoots were transferred to MS media containing indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA) at different concentrations (1, 2, 3, and 4 mg/l). The optimum auxin applications for root and plant development were determined by examining the rooting percentage (%, RP), root number per shoot (RN /S), root length (RL), shoot length (SL), and plant fresh weight (FW). As a result of the study, it was found that the media containing 3 mg/l and 2 mg/l BA were the most suitable shoot propagation media for G. lutea and G. boissieri, respectively. The media containing 4 mg/l IBA and 1 mg/l AgNO3 gave the highest rooting and growth parameters for G. lutea, while G. boissieri had the best rooting and growth values in media supplemented with 2 mg/l NAA. In addition, G. boissieri was found to be superior to G. lutea in both shoot propagation and rooting.

Effect of the Root Endophytic Fungus Piriformospora indica on Strawberry Growth, Fruit Quality and Physiological Traits Under Elevated Electrical Conductivity

Strawberries are sensitive to abiotic stresses such as salinity, high levels of electrical conductivity, and nutrient imbalances. The mutualistic endophytic fungus Piriformospora indica has significant potential to be used in improving crop production under adverse conditions, with a large host range. However, greenhouse production requires novel tactics to improve the efficiency of saline water irrigation in areas with limited freshwater resources. This study was conducted at the National Research and Development Center for Sustainable Agriculture (Estidamah), KSA, to investigate the impact of P. indica colonization on the growth, photosynthesis traits, productivity, and fruit quality of Fragraria x ananassa Duch cv. Festival strawberry grown in pots and irrigated with two electrical conductivity (EC) nutrient solutions of 1.5 and 3.0 dS/m. The results showed that higher-EC-nutrient solution clearly reduced growth and early yield and improved vitamin C, TSS, and anthocyanin of strawberry in comparison to low-EC-nutrient solution. On the other hand, P. indica colonization significantly increased plant height, shoot fresh weight, root length, and early yield of plants grown under high-EC-nutrient solution with no impact on fruit quality. Obviously, the symbiosis between strawberry roots and P. indica enhanced chlorophyll content, photosynthetic rate, stomatal conductance, and transpiration rate, as well as antioxidant activity such as proline, malondialdehyde, catalase, superoxide dismutase, and peroxidase under higher-EC-nutrient solution. Our study indicated that P. indica might be used as a sustainable tool for strawberry production in arid and semiarid zones, to mitigate the negative impacts of higher-EC-nutrient solution.

Photoperiodic Effect on Growth, Photosynthesis, Mineral Elements, and Metabolome of Tomato Seedlings in a Plant Factory

The duration of light exposure is a crucial environmental factor that regulates various physiological processes in plants, with optimal timing differing between species and varieties. To assess the effect of photoperiods on the growth and metabolites of a specific truss tomato cultivar, three photoperiods (12 h, 16 h, and 20 h) were tested in a plant factory. Growth parameters, including plant height, stem diameter, fresh and dry weights of shoots and roots, photosynthetic characteristics, mineral content, and metabolome profiles, were analyzed under these conditions. The results indicated that prolonged light exposure enhanced plant growth, with the highest photosynthesis and chlorophyll content observed under a 20 h photoperiod. However, no significant correlation was observed between the photoperiod and the mineral element content, particularly for macro minerals. Metabolome analysis revealed that different photoperiods influenced the accumulation of metabolites, particularly in the lipid metabolism, amino acid metabolism, and membrane transport pathways. Long periods of light would enhance photosynthesis and metabolism, improving the rapid growth of tomato seedlings. Overall, this study provides a theoretical basis for understanding the responses of truss tomato cultivars to varying photoperiods in plant factories and proposes an optimizable method for accelerating the progress of tomato seedling cultivation.

Synthesis and mechanism of Mg/Al layered double oxides-silica nanocomposites for sustainable multi-nutrient delivery in agricultural applications

Potassium (K), urea (N), phosphate (P), and selenite (Se) are widely used in modern agriculture for improvement of crop yield and quality. However, traditional fertilizer suffers from poor fertilizer utilization efficiency and noncontrollable slow-release behavior. To improve nutrient utilization, we developed a layered double oxides-silica (LDO@Si) based on calcined Mg/Al layered double hydroxide-silica nanocomposites (LDH@Si), for slow release of K, N, P, and Se to crops. In this study, SEM, XRD, FT-IR, XPS, BET, and TGA were employed to analyze the structure, morphology, and microstructures of the samples. Results show that LDH@Si successfully transforms into LDO@Si after calcination, where LDO is mainly connected to silica via M-O-Si bonds. Furthermore, after K, P, and Se loading, the LDH@Si structure was successfully restored, indicating that phosphate and selenite ions have been effectively embedded in the inner layer of LDH. K ions are firmly fixed to the material surface via M-O-K bonds. After urea introducing, the pore structure of the material was completely filled, and excess urea formed a urea layer on the surface of the material. LDO@Si can continuously release nutrients into water through diffusion and LDO@Si dissolution for up to 240 h. Compared with chemical fertilizers, LDO@Si based slow-release fertilizer (CRSF2) significantly improves plant fresh weight, dry weight and chlorophyll content, increasing them by 13.91 %–23.13 %, 18.20 %–34.40 % and 2.24 %–14.81 %, respectively. Furthermore, a modest increase in the levels of nutrients N, P, and K is observed, while the Se concentration in plants treated with CRSF2 demonstrates significant enhancements of 9.57 %, 72.49 %, and 50.97 % compared to control treatments. These results illustrate the potential agricultural application of LDO@Si as a slow-release fertilizer for improving crop yields while minimizing the required amount of fertilizer.

Effect of Palm Oil Mill Effluent (Pome) Concentration and Soil Type on the Growth of Oil Palm (Elaeis guinensis Jacq) in Pre-Nursery

The place of research was carried out at Educational and Research Garden of the STIPER Agricultural Institute which is located in Maguwoharjo Village, Depok District, Sleman Regency, Yogyakarta, Indonesia. The research location is located 118 meters above sea level. This research was conducted between March to June 2024. Two components make up the factorial experiment used in the Complete Randomized Design (CRD). The soil type had three levels: A1 = latosol soil, A2 = regusol soil, and A3 = grumusol soil, was the first factor. Palm Oil Mill Effluent (POME) concentration was the second factor. It was consisted into four levels: X1 was the control (NPK fertilizer), X2 was 150 ml per polybag, X3 was 300 ml per polybag, and X4 was 450 ml per polybag. So there were 12 treatment combinations (3 x 4 = 12) with 4 repetitions, so all the experiment 48 plants. Analysis of variance (ANOVA) was applied at the significant level of 5% to check the observation data. Duncan's Multiple Range Test (DMRT) was then used at a noticeable 5% level if there was significant difference. The weight parameters of fresh roots and the amount of chlorophyll in the leaves are influenced by the interaction of the soil and POME concentration. The composition, regusol soil and POME 450 ml/polybag provide an optimal combination of treatments. The type of soil has an impact on the weight of fresh and dry crowns, the number of leaves, the chlorophyll content in the leaves, the volume of roots, and the height of the plant. The best type of soil is regusol soil. Plant height, root volume, fresh and dry crown weight, and leaf chlorophyll content are all affected by POME concentration. The concentration of POME 450 ml/polybag showed the best results.

The herbicidal activity and mechanism of Talaromyces purpureogenus CY-1 metabolites.

Studies have shown that Talaromyces can produce a large number of secondary metabolites in its metabolic process, many of which have good insecticidal, antibacterial, antitumor, antiviral and other biological activities. In order to explore the herbicidal activity and mechanism of Talaromyces purpureogenus CY-1, we determined the inhibitory effect of the fermentation broth of the CY-1 strain on weeds, identified the major active components, and further investigated the herbicidal mechanism. The results showed that CY-1, with IC50 values of 5.40 g/L and 4.39 g/L, respectively, exhibited good herbicidal activity against Xanthium sibiricum and Amaranthus lividus. Spraying CY-1 strain fermentation broth on redroot pigweed resulted in plant protection efficiencies and fresh weight protection efficiencies of 83.7% and 87%, respectively. The active component identified in the broth was 2-(3-hydroxybenzoyloxy) acrylic acid. Treatment of Amaranthus lividus with 2-(3-hydroxybenzoyloxy) acrylic acid resulted in trends of increasing superoxide dismutase activity, peroxidase activity, respiratory rate, cytochrome oxidase activity, and soluble protein content, followed by a decrease. Peroxidase activity, relative conductance and malondialdehyde content gradually increased, while acetyl lactate synthase and glutamine synthetase initially decreased and gradually returned to normal. The soluble sugar content showed a gradual decrease. The findings of the present study indicate that 2-(3-hydroxybenzoyloxy) acrylic acid can induce oxidative stress in plants, damaging plant cell walls, weakening respiratory activity, inhibiting protein synthesis and sugar metabolism, ultimately leading to weed wilting and death. © 2024 Society of Chemical Industry.

The effect of organic manure on the production of jute (Corchorus olitorius)

Field experiments to investigate the effect of different sources of organic manure (Swine manure (SM), Poultry manure (PM), Cattle manure (CM), Rabbit urine manure (RUM)) on production of Jute (Corchorus olitorius) was carried out. The experimental site was cleared and cultivated (tilled and fertilized). Treatments were laid out before planting at once, there was no re-application of treatment. Data were collected on growth and yield parameters (Foliage, Plant height and Plant fresh weight). There was a significant difference in the foliage of Corchorus olitorius treated with SM, PM, CM, and RUM when compared with the control group. The foliage of Corchorus olitorius with PM treatment is significantly higher (P<0.05) compared with other organic manure treatment except for CM. Significant increase (P<0.05) in the height of Corchorus olitorius was obvious in all the treatment group when compared with control. There is no significant difference (P>0.05) in the height of jute across the organic manure treatment groups (SM, PM and RUM) but there is a significant increase in the plant height of PM when compared CM. SM, PM and RUM significantly increase the fresh weight of the plant when compared with the control and CM. There was a numerical increase in the plant fresh weight of PM when compared to other treatment groups. From this experimental study, PM consistently increased the number of leaves, the fresh plant weight and plant height significantly and it is recommended for Corchorus olitorius production.

Morphological, biochemical and genotoxic effects of non-ionizing radiation at 1800MHz and 2400MHz frequencies in Allium cepa L.

The frequent use of electronic devices in daily lives, predominantly reliant on non-ionizing radiation, has increased the prevalence of electromagnetic radiation (EMR) in natural environment. In light of this, effects of EMR at frequencies of 1800MHz and 2400MHz characterized by a power of 10.0 dBm (0.01 W), across varying exposure durations of 1h/day, 2h/day, 4h/day, 6h/day, and 8h/day for 7days, in Allium cepa L. were studied. The effects of the treatment on the morphological features (root length, fresh weight, and dry weight of roots) and biochemical characteristics (protein content and antioxidative enzymes, namely, ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST), guaiacol peroxidase (POD), catalase (CAT), and dehydroascorbate (DHAR)) were studied in roots and bulbs of Allium cepa L. Further genotoxicity for different exposure periods at both frequencies was also conducted. Prolonged exposure to electromagnetic radiation (EMR) at both frequencies was found to reduce root length, fresh weight, and dry weight of plant. Furthermore, significant effects were observed on protein content, indicating a reduction with prolonged exposure duration. Investigation into the activities of antioxidative enzymes such as APX, GR, GST, DHAR, CAT, SOD, and POD at a frequency of 1800MHz and 2400MHz in roots and bulbs demonstrated a significant enhancement in enzyme activity during 6h/day and 8h/day exposure periods. Additional investigation during genotoxicity studies demonstrated the induction of chromosomal aberrations in the root tip cells of the Allium cepa L. plant test system. The current study revealed the initiation of oxidative stress and genotoxicity resulting from long-term exposure to electromagnetic radiation in the studied plant test systems.

Deciphering the Biocontrol Potential of Trichoderma asperellum (Tv1) Against Fusarium-Nematode Wilt Complex in Tomato.

The study assessed Trichoderma spp. as a biocontrol agent for managing wilt-nematode complex in tomato crops, aiming to mitigate yield losses. Fusarium sp. and Meloidogyne sp. were isolated from infected plant samples and confirmed molecularly and morphologically as Fusarium oxysporum f.sp. lycopersici and Meloidogyne incognita. Four Trichoderma spp. procured from the Department of Plant Pathology, Tamil Nadu Agricultural University, India were identified molecularly as Trichoderma asperellum (Tv1), Trichoderma asperelloides (Tasd1), Trichoderma harzianum (Th1), and Trichoderma koningiopsis (Tk1) utilizing ITS and TEF1 primer pairs. Among them, Tv1 effectively inhibited the mycelial growth of pathogen isolates. Furthermore, crude metabolite of Tv1 exhibited similar effects. The mortality rate of M. incognita J2s ranged from 90.48% to 100% after 24-72 h of incubation and inhibition percentage of egg hatching reached 90.20%. The shoot length, root length, fresh weight, and dry weight of the tomato plants treated with Tv1 conidia were increased. In a pot experiment, Tv1 treatment reduced disease incidence by 64.57%, comparable to carbendazim treatment (82.32%). Tv1-treated plants had fewer root galls, egg masses, and J2s per 100 g of roots than the inoculated-untreated control plants. The root-knot index (RKI) was significantly lower in plants treated with carbafuran (1.98 ± 0.047) and Tv1 (3.06 ± 0.086) than in control (4.47 ± 0.109). The bio-control efficiency of Tv1 against M. incognita was 21.04%, and the nematodes' reproductive factor (RF) declined to 0.53 in Tv1 treatment group. Based on the findings above, it was established that Tv1 effectively controlled nematode populations and wilt disease when applied in soil.

Resource recycling of nitrogen and phosphorus from waste adsorbents: Implications for soil improvement and plant growth

The enrichment of nitrogen and phosphorus in sewage poses a significant risk of resource loss if not effectively recovered and utilized. This study investigates the sustainable management and conservation of these vital nutrients through the recycling of saturated Mg-loaded adsorbents as soil additives for mint (Mentha spicata) cultivation. The effects of recycled waste adsorbents were compared with new adsorbents, commercial compound fertilizers, and struvite, using a control check (CK) to assess their impact on soil nutrient content and plant growth parameters. Results showed that waste adsorbents significantly enhanced soil nutrient content compared to CK. Although nutrient levels in the first 30 days were lower than with commercial fertilizers, a reversal was noted between 30 and 60 days, indicating a slower nutrient release rate. Furthermore, the germination rate and leaf width of mint increased by 13.34 % and 25.38 %, respectively. Significant improvements in plant height, fresh weight, and dry weight demonstrated positive effects on growth. This study confirms the feasibility of recycling waste Mg-loaded adsorbents for the sustainable reutilization of nitrogen and phosphorus resources from wastewater, contributing to enhanced soil health and sustainable agricultural practices.

Preliminary Insights into Sustainable Control of Solanum lycopersicum Early Blight: Harnessing Arbuscular Mycorrhizal Fungi and Reducing Fungicide Dose

Tomato (Solanum lycopersicum L.) production is constantly threatened by several fungal pathogens, such as Alternaria solani, the causal agent of early blight disease. In this study, a greenhouse experiment was set up to evaluate the biocontrol ability of arbuscular mycorrhizal fungi (AMF) against A. solani in the presence of reduced doses of fungicides (i.e., captan and copper oxychloride). Disease severity, plant growth traits, chlorophyll and phosphorus content, phenolic compounds, and antioxidant activity were assessed. The effects of fungicide dose on AMF were investigated by root colonization, spore density, and mycorrhizal dependence evaluation. AMF-inoculated and fungicide-treated plants reduced disease severity compared to fungicide-treated and non-mycorrhizal plants, in most cases, regardless of the fungicide dose. AMF improved plant growth, especially when combined with copper oxychloride. However, plant fresh weight decreased in plants treated with the lowest dose of captan (25 g 100 L−1). Overall, AMF colonization decreased in plants with high fungicide doses, while the leaf color parameters did not show differences between treatments. The results suggest reducing the fungicide dose using AMF is possible, particularly for copper oxychloride. Further studies will be required to confirm these data. This integrated approach could offer a sustainable alternative to decrease the use of chemical control.

Genetic variability, heritability, and genetic advance estimates in selected groundnut genotypes (<i>Arachis hypogaea</i> L.) for agro-morphological and yield traits.

Groundnuts (Arachis hypogaea L.), is a crucial food and oil crop in the tropics, require genetic variability assessment for efficient breeding programs using appropriate parameters like genetic coefficient of variation, heritability estimates, association among yield related traits and genetic advance. This study aims to identify genotypes with desirable traits to improve yield and adaptability, contributing to better crop improvement strategies. The study used a randomized complete block design with three replications, consisting of fourteen selected genotypes. Quantitative traits were used to estimate genetic variability parameters, heritability, and genetic advance. The analysis of variance revealed significant differences (p < 0.005) for all studied traits, indicating greater variability in genotypes. Characters exhibited different levels of variability, heritability, and genetic advance among the genotypes. The estimate of the broad-sense heritability ranged from 13.33% to 33.33% for canopy spread and days to maturity respectively. Low to high phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) were recorded. The study found that traits such as number of branches, pods per plant, fresh and dry plant weight, kernel yield, pod yield, and shelling percentage showed high genotypic coefficient of variation, moderate broad sense heritability, and high genetic advance. These traits can be beneficial for groundnut improvement through effective phenotypic selection, resulting in high expected genetic gain. The study found that pod yield is significantly correlated with kernel yield, grain length, grain width, 100-seed weight, fresh and dry 100-pod weight, and number of pods per plant. Kernel yield is positively correlated with the number of branches per plant, pod per plant, fresh and dry 100-pod weight, 100-seed weight, grain length, and grain width. In addition, the first four principal components accounted for 90.37% of total variation in all morphological traits.

Comparing the individual and combined effects of nano zinc and conventional zinc fertilization on growth, yield, phytochemical properties, antioxidant activity, and secoisolariciresinol diglucoside content in linseed

Zinc sulfate (ZnSO4), a conventional Zn fertilizer, is widely used due to its high solubility and ease of application. In contrast, nano Zn represents an innovative approach, utilizing nanoscale particles to enhance Zn bioavailability and uptake efficiency. This study compares these two Zn fertilizers regarding their impact on plant growth, yield, Zn uptake, and overall crop quality. In our study, we explored the potential of nano Zn and ZnSO4 by applying two different doses of each (100, 1000 ppm ZnO NP and 30, 50 kg ha−1 ZnSO4) both individually and in combination, to linseed accession. The results obtained showed the potential of nano Zn over conventional Zn fertilizer in terms of enhanced linseed growth and yield together with greater antioxidants enzyme, oil content, protein content, Zn accumulation, secoisolariciresinol diglucoside (SDG) content, and the accumulation of bioactive metabolites. Nanoscale ZnO (with particle size less than 100 nm) at a 1000 ppm concentration sped up growth, yield, increased SDG content, and antioxidant activity. However, when nano Zn (1000 ppm) was applied in combination with ZnSO4 (30 kg ha−1), it maximally enhanced plant fresh and dry weight, photosynthesis, and yield compared to their individual treatment. The combined application increased seed yield by 4.55 folds compared to the control. The treated plants were assessed for SDG content using liquid chromatography-mass spectrometry analysis (LC-MS), which showed maximum increase with 1000 ppm ZnO NP. SDG is a type of lignan known for their antioxidant properties and potential health benefits paving way for its pharmaceutical importance.

Mitigating Salinity Stress in Cotton (Gossypium hirsutum L.) with K-humate and Iron Oxide Nanoparticles

Salinity stress poses a major challenge to plant growth and development, causing problems like osmotic stress, ion toxicity, and nutrient imbalances. These issues lead to reduced photosynthesis and early aging of plants. In this study, we explored the potential of potassium humate (Kh) and iron oxide nanoparticles (Fe (II,III) oxide-NPs) to help cotton plants (Gossypium hirsitum L.) cope with saline conditions. We examined various growth parameters such as plant height, leaf number, fresh and dry weights of leaves and roots, leaf area, chlorophyll content (SPAD values), and relative water content (RWC). Our findings showed that salinity stress significantly decreased plant height, leaf number, fresh leaf weight, dry leaf weight, leaf area, and RWC. However, the application of Fe (II,III) oxide-NPs and Kh helped mitigate these negative effects. Notably, the combination of Fe (II,III) oxide-NPs and Kh resulted in the highest plant height under saline conditions. Kh alone was particularly effective in increasing leaf number and fresh leaf weight, even under stress. In conclusion, both Fe (II,III) oxide NPs and Kh proved to be effective in enhancing cotton plant resilience to salinity stress, highlighting their potential use in sustainable agriculture in saline and arid regions. These insights can help develop strategies to improve plant growth and yield under challenging environmental conditions.

Improving yield and irrigation water productivity of green beans under water stress with agricultural solid waste-based material of compacted rice straw as a sustainable organic soil mulch

AbstractThis research aimed at water saving in irrigation by applying deficit irrigation using two strategies, standard drip and partial root drying (PRD), while applying organic and plastic mulch over two growing seasons of green beans. A field experiment was conducted in 2022 and 2023, using four irrigation treatments supplying 100% of the irrigation requirement (IRg), 75% IRg, 50% IRg, and 50% IRg—PRD, and four soil mulching treatments: uncovered soil (UC), plastic mulch (PM), rice straw mulch (RSM), and compacted rice straw mulch (CRSM). The combined effect of deficit irrigation strategies and soil mulching showed that the maximum irrigation water productivity (IWP) of 5.56 kg m−3 was achieved under 50% IRg—PRD & CRSM for both growing seasons, followed by 50% IRg—PRD & RSM and 50% IRg—PRD & PM, with 5.19 and 4.96 kg m−3, respectively. The highest yield of 8936 kg ha−1 was achieved with 50% IRg—PRD & CRSM, followed by 8914 kg ha−1 and 8898 kg ha−1 with 100% IRg & CRSM and 75% IRg & CRSM, respectively. The lowest yield of 6009 kg ha−1 was obtained with 50% IRg & UC. The highest soil moisture content was observed under 100% IRg & CRSM. The application of organic mulches was found to be particularly effective in conserving soil moisture due to enhanced infiltration, improved retention capacity, and suppression of weed growth, ultimately fostering optimal crop development and higher yield. The results of soil temperature variations beneath soil mulches showed that CRSM is effective in alleviating plant water stress, lowering the temperature below the cover and reducing water loss through evaporation from the soil surface. The combination of 50% IRg—PRD & CRSM produced plants with enhanced plant height, fresh and dry weight, leaf area, pod length, and green bean weight, as well as the highest vegetative growth indices. Generally, the organic mulching increased soil temperature, soil moisture, IWP, and green bean production.

Effect of Pinching on Growth, Yield, and Quality of African Marigold (Tagetes erecta L.) cv. Pusa Narangi Gainda

Marigold is a very common and a hardy flower crop cultivated throughout the world. Pinching is an important intercultural practice commonly followed in cultivation of marigold however, this needs to be standardized for getting higher yields in commercial cultivation. Hence, the present investigation was conducted at Rajasthan Agricultural Research Institute, Durgapura, Jaipur, Division of Horticulture, during 2020-21 and 2021-22 to standardize the commercial pinching practice for getting higher productivity. Three levels of pinching (no pinching, single at 20 days and double at 20 and 40 days after transplanting) were used in the course of the research arranged using a factorial randomized block layout with three replications applied to African marigold variety Pusa Narangi Gainda. The results revealed that treatment P0 (No pinching) was discovered to be the finest in increment in height of plant, early flower bud initiation flower opening and treatment P2 (Double pinching) in boosting spread of plant, primary branches, length of leaf, leaf width, leaf area, plant fresh weight, plant dry weight, total biomass, root- shoot ratio, 50% flowering, flower diameter, stalk and ray floret length, fresh and dry flower weight, mass and number of flower per plant, flower yield additionally improved quality characteristics like chlorophyll and xanthophyll content in marigold. Hence, it might be concluded that application of double pinching can be helpful and recommended in improving yield as well as quality characteristics in commercial cultivation of marigold.

Elevated concentrations of soil carbon dioxide with partial root-zone drying enhance drought tolerance and agro-physiological characteristics by regulating the expression of genes related to aquaporin and stress response in cucumber plants

Water scarcity and soil carbon dioxide elevation in arid regions are considered the most serious factors affecting crop growth and productivity. This study aimed to investigate the impacts of elevated CO2 levels (eCO2 at rates of 700 and 1000 ppm) on agro-physiological attributes to induce drought tolerance in cucumbers by activating the expression of genes related to aquaporin and stress response, which improved the yield of cucumber under two levels of irrigation water conditions [75% and 100% crop evapotranspiration (ETc)]. Therefore, two field experiments were conducted in a greenhouse with controlled internal climate conditions, at the Mohamed Naguib sector of the national company for protected agriculture, during the winter seasons of 2021–2022 and 2022–2023. The treatments included eCO2 in soil under normal and partial root zoon drying (PRD, 100% ETc Full irrigations, and 75% ETc). All the applied treatments were organized as a randomized complete block design (RCBD) and each treatment was replicated six times. Untreated plants were designed as control treatment (CO2 concentration was 400 ppm). The results of this study showed that elevating CO2 at 700 and 1000 ppm in soil significantly increased plant growth parameters, photosynthesis measurements, and phytohormones [indole acetic acid (IAA) and gibberellic acid (GA3)], under partial root-zone drying (75% ETc) and full irrigation conditions (100% ETc). Under PRD condition, eCO2 at 700 ppm significantly improved plant height (13.68%), number of shoots (19.88%), Leaf greenness index (SPAD value, 16.60%), root length (24.88%), fresh weight (64.77%) and dry weight (61.25%) of cucumber plant, when compared to untreated plants. The pervious treatment also increased photosynthesis rate, stomatal conductance, and intercellular CO2 concentration by 50.65%, 15.30% and 12.18%; respectively, compared to the control treatment. Similar findings were observed in nutrient concentration, carbohydrate content, Proline, total antioxidants in the leaf, and nutrients. In contrast, eCO2 at 700 ppm in the soil reduced the values of transpiration rate (6.33%) and Abscisic acid (ABA, 34.03%) content in cucumber leaves compared to untreated plants under both water levels. Furthermore, the results revealed that the gene transcript levels of the aquaporin-related genes (CsPIP1-2 and CsTIP4) significantly increased compared with a well-watered condition. The transcript levels of CsPIP improved the contribution rate of cell water transportation (intermediated by aquaporin’s genes) and root or leaf hydraulic conductivity. The quantitative real-time PCR expression results revealed the upregulation of CsAGO1 stress-response genes in plants exposed to 700 ppm CO2. In conclusion, elevating CO2 at 700 ppm in the soil might be a promising technique to enhance the growth and productivity of cucumber plants in addition to alleviating the adverse effects of drought stresses.

Respon Pertumbuhan Bibit Single Bud Tanaman Aglaonema Varietas Donna Carmen Terhadap Aplikasi Zat Pengatur Tumbuh dan Pupuk Daun

Ornamental plants now become a trend among the public. The hobby of growing ornamental plants causes the demand for ornamental plant is increased day by day, especially the Aglaonema ornamental plant. In general, the propagation of Aglaonema plants is carried out by using separation of tillers and stem cuttings of 4-7 segments. Due to the high demand for Aglaonema plants, a new method of plant propagation is needed, in order to produce more plants in a short time, by using the single bud nursery method. Aglaonema stem cuttings are difficult to do because Aglaonema plants has bud's dormancy period. Dormancy of Aglaonema plant buds is triggered by apical dominance. The way to break bud dormancy on Aglaonema cuttings is using zpt auxin (Rootone-f). In addition, plants also need nutrients to grow, by providing fertilizer. Fertilization for ornamental plants generally uses foliar fertilizer (Gandasil D). This research was conducted using a Randomized Block Design (RBD) which consisted of 2 factors, the first factor zpt auxin (Rootone-f) which consisted of 4 levels (0 ppm, 100 ppm, 200 ppm, 300 ppm), and the second factor was Gandasil D foliar fertilizer which consisted of 3 treatment levels (0 g/l, 1 g/l, 2 g/l) so there are 12 treatment combinations. Each combination was repeated 3 times, thus forming 36 experimental units. The data were analyzed by analysis of variance (ANOVA) to determine the effect of the treatment and those whose effects were significantly different were further tested using the Ducan Multiple Range (DMRT) with a 95% confidence level. The combination of auxin zpt treatment (Rootone-f) with a concentration of 200 ppm and Gandasil D foliar fertilizer with a concentration of 2 g/l showed the best treatment combination on the parameters of observing fresh weight of Aglaonema plants. The concentration of zpt auxin (Rootone-f) 200 ppm gave the highest average value of shoot length, number of leaves, root length, and number of roots of Aglaonema plants. The concentration of Gandasil D 2 g/l foliar fertilizer gave the highest average leaf length and leaf width of Aglaonema plants.

Influence of indole acetic acid and trehalose, with and without zinc oxide nanoparticles coated urea on tomato growth in nitrogen deficient soils

Nitrogen deficiency in low organic matter soils significantly reduces crop yield and plant health. The effects of foliar applications of indole acetic acid (IAA), trehalose (TA), and nanoparticles-coated urea (NPCU) on the growth and physiological attributes of tomatoes in nitrogen-deficient soil are not well documented in the literature. This study aims to explore the influence of IAA, TA, and NPCU on tomato plants in nitrogen-deficient soil. Treatments included control, 2mM IAA, 0.1% TA, and 2mM IAA + 0.1% TA, applied with and without NPCU. Results showed that 2mM IAA + 0.1% TA with NPCU significantly improved shoot length (~ 30%), root length (~ 63%), plant fresh (~ 48%) and dry weight (~ 48%), number of leaves (~ 38%), and leaf area (~ 58%) compared to control (NPCU only). Additionally, significant improvements in chlorophyll content, total protein, and total soluble sugar, along with a decrease in antioxidant activity (POD, SOD, CAT, and APX), validated the effectiveness of 2mM IAA + 0.1% TA with NPCU. The combined application of 2mM IAA + 0.1% TA with NPCU can be recommended as an effective strategy to enhance tomato growth and yield in nitrogen-deficient soils. This approach can be integrated into current agricultural practices to improve crop resilience and productivity, especially in regions with poor soil fertility. To confirm the efficacy of 2mM IAA + 0.1% TA with NPCU in various crops and climatic conditions, additional field studies are required.

Effects of copper sulphate stress on the morphological and biochemical characteristics of Spinacia oleracea and Avena sativa

Plants are subjected to various biotic and abiotic stresses that significantly impact their growth and productivity. To achieve balanced crop growth and yield, including for leafy vegetables, the continuous application of micronutrient is crucial. This study investigates the effects of different concentrations of copper sulphate (0, 75, 125, and 175 ppm) on the morphological and biochemical features of Spinacia oleracea and Avena sativa. Morphological parameters such as plant height, leaf area, root length, and fresh and dry weights were optimized at a concentration of 75 ppm copper sulfate. At this concentration, chlorophyll a & b levels increased significantly in Spinacia oleracea (462.9 and 249.8 \U0001d707\U0001d454/\U0001d454), and Avena sativa (404.7 and 437.63\U0001d707\U0001d454/\U0001d454). However, carotenoid content and sugar levels in Spinacia oleracea were negatively affected, while sugar content in Avena sativa increased at 125 ppm (941.6 µg/ml). Protein content increased in Spinacia oleracea (75 ppm, 180.3 µg/ml) but decreased in Avena sativa. Phenol content peaked in both plants at 75 ppm (362.2 and 244.5 µg/ml). Higher concentrations (175 ppm) of copper sulfate reduced plant productivity and health. Plants exposed to control and optimal concentrations (75 and 125 ppm) of copper sulpate exhibited the best health and growth compared to those subjected to higher concentrations. Maximum plant height, leaf area, root length, fresh and dry weights were observed at lower concentrations (75 and 125 ppm) of copper sulfate, while higher concentrations caused toxicity. Optimal copper sulfate levels enhanced chlorophyll a, chlorophyll b, total chlorophyll, protein, and phenol contents but inhibited sugar and carotenoid contents in both Spinacia oleracea and Avena sativa. Overall, increased copper sulfate treatment adversely affected the growth parameters and biochemical profiles of these plants.