New donor-π-acceptor (D-π-A) type quinoxaline derivatives with carbazole (ECVQ), triphenylamine (TPVQ) and phenothiazine (EPVQ) moieties as electron-donating groups were synthesized by standard Knoevenagel condensation reactions with good yields. Their fluorescent emission spectra in both solution and the solid state and mechanofluorochromic behavior was compared and studied. The results indicated that these D-π-A type molecules showed unique intramolecular charge transfer (ICT) properties. The extent of the ICT of the three compounds increased with the sequence of ECVQ < TPVQ < EPVQ. It is interesting that TPVQ and EPVQ exhibited reversible mechanofluorochromism upon the treatment of grinding and fuming. TPVQ showed low emission color change (a spectral shift of 12 nm), and EPVQ revealed high contrast MFC with a large spectral change of 53 nm, which might be originated from bending configuration of EPVQ with phenothiazine group and efficient ICT progress. By sharp contrast, ECVQ did not exhibit MFC behavior. The reason was that the planar molecular conformation and weak ICT degree of ECVQ. This work demonstrated dynamic control of the fluorescent emission and mechanofluorochromic behavior by using different electron donating moieties of D-π-A type quinoxaline derivatives.