The additive manufacturing technology has made its debut in the industrial field about 30 years ago, when prototyped parts were usually used at the 3D printing stage during fabrication, to give the end customer a truthful concept of how a part will looks when conventional manufacturing techniques were used for final part fabrication. Because of the increasing demand for non-toxic, biodegradable materials and products, human society is always searching for new materials with specific applications, which are able to fulfill the above-mentioned requirements. Consequently, it is essential to identify the qualities of these materials and their behavior when subjected to various external factors, in order to find their optimal solutions for application in various domains. Manufacturing parts from biodegradable materials by 3D printing represents a major concern of industry specialists. The 3D printing process involves several parameters whose influence on the sample functional characteristics is a topical issue. In this paper are determined influences of certain technological parameters (thickness of the layer, filling speed, and part orientation on the printing bed) on some mechanical properties (tensile strength, structure, thermal analysis by DSC, and friction coefficient). Experiments were performed on specimens made of three materials: PLA, HD PLA Green, and Impact PLA Gray. A complete factorial experimental plan was used with three input parameters on two levels. Each experiment was repeated three times following the process stability. The obtained mean values of the tensile test were used in the analysis. The analysis was performed with the MiniTab application, which allowed the parameters hierarchy by influencing each mechanical characteristic studied, model development, and optimum values setting.