Comfort is the prime need in clothing. It is affected by the properties of fibers, yarns, and fabrics. A good understanding of these factors is essential in the design and conception of functional clothes. Fabric design serves as a manufacturing tool to meet several end-use requirements. The construction specifications and design of knitted fabrics greatly influence the comfort level of the end-use garment. This study aims to investigate the effect of the knitting stitch type and the dyeing process on the clothing comfort of knitted fabrics. For this purpose, five structures were prepared by combining plain, float and tuck stitches: plain jersey, cross miss 1 × 1, single cross tuck, weft lock knit and Lacoste. Then, yellow, and black dyeing were applied. The investigated wear comfort properties were the drying time, the air permeability and the relative water vapor permeability. Tests were conducted on the finished and on domestically washed fabrics. The data obtained were statistically evaluated with Pearson correlation, paired t-test, analysis of variance analysis and the Tukey test. The results showed that tucked fabrics have the longest drying time combined with the lowest air and water vapor permeability. Black variants showed better drying behavior and higher water vapor permeability. Analysis of variance results showed that the structure has a significant effect on clothing comfort. However, the dyeing process exerts a significant influence only on the drying time and the relative water vapor permeability. The paired t-test results indicate that the domestic washing cycles significantly change the drying time and the air permeability of knitted fabrics.