Event Abstract Back to Event To study the contribution of activated CD4+ and CD8+T cells arisen from B-T constructs of F1 and V antigens of Yersinia pestis as an improved vaccine Geetanjali Gupta1 and D N. Rao2* 1 All India Institute of Medical Sciences, India 2 All India Institute of Medical Scinces, Biochemistry, India Yersinia pestis, a Gram negative bacterium, is the etiological agent of pneumonic and bubonic plague. The current efforts are aimed at evaluating whether our vaccine protocol primes memory T cells that rapidly produce IFN-γ and/or perforin in response to peptide or conjugate stimulation. In the present study, the secretion of IFN-γ and IL-4 by splenocytes, stimulated by different constructs of B and T cell epitopes of F1 and V antigens, was measured by ELISpot assay. We also measured perforin and IFN-γ expression as a function of cell mediated immunity by flow cytometry. We observed that three B–T constructs of F1 and seven B–T constructs of V antigens produced a high number of IFN-γ secreting cells as compared to native antigen and a low number of IL-4 secreting cells. B–T conjugates of F1 and V antigens showed significantly high (p ‹ 0.001) percentage of CD4+ IFN-γ+ cells as compared to CD8+ IFN-γ+ cells. Our study demonstrated that IFN-γ is an effector molecule of activated CD4+ and CD8+T cells arisen from B–T constructs of F1 and V antigens for the antibacterial activity as an improved plague vaccine. Keywords: mucosal immunity, microspheres, conjugate vaccine, Flow Cytometry, Elispot Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Gupta G and Rao DN (2013). To study the contribution of activated CD4+ and CD8+T cells arisen from B-T constructs of F1 and V antigens of Yersinia pestis as an improved vaccine. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00109 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. D N Rao, All India Institute of Medical Scinces, Biochemistry, New Delhi, New Delhi, 110029, India, dnrao311@rediffmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Geetanjali Gupta D N Rao Google Geetanjali Gupta D N Rao Google Scholar Geetanjali Gupta D N Rao PubMed Geetanjali Gupta D N Rao Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.