The study was carried out to determine the appropriate age and route of vaccination with duck plague vaccine (LRI, Mohakhali) in experimentally reared local ducks during the period from September 2006 to May 2007. A total of 90 local ducklings were divided into eight groups namely A, C, D and E for vaccinated parent origin and B, F, G and H for non-vaccinated parent origin. Again Group C, D, E, F, G and H were divided into subgroups C1 & C2; DÂ1& DÂ2; E1& E2; F1 & F2; G1 & G2; H1 & H2 respectively and contained 5 ducklings each. Group A and B contained 15 ducklings in each group and were used as unvaccinated control and also for studying persistence of maternally derived antibody level. Highest mean MDA titre in ducklings of Group A was found 53.33 ± 4.03 at the age of day 1 that declined to a negligible level (â¤4) at the age of day 21 and highest mean MDA titre in ducklings of Group B was found 29.86 ± 1.45 at the age of day 1 that declined to a negligible level (â¤4) at the age of day 16. Ducklings of different sub-groups were vaccinated with duck plague vaccine at day 14, day 21 and day 28 through intramuscular (breast) and subcutaneous route at the dose rate of 1ml per duckling. Sera were collected from vaccinated ducklings on day 7, day 14 and day 21 after vaccination and antibody titre was measured by passive hemagglutination test. Among the vaccinated subgroups, the highest mean PHA titre was found 89.60 ± 15.67 and 83.20 ± 19.20 in subgroups E1 (vaccinated at day 28) and F1 (vaccinated at day 14) respectively. At challenge, ducklings of vaccinated subgroups showed 100% protection except C1 and C2 (showed 80% protection) and control subgroups showed 0% protection. From the results of protection test it may be concluded that both intramuscular (breast) and subcutaneous routes are equally suitable for duck plague virus vaccination in ducklings and the optimum age for vaccination to ducklings originated from vaccinated parent to duck plague vaccine might be at day 21 or 28 and ducklings originated from non-vaccinated parent at day 14 or 21 instead of usual schedule of day 28. Key words: Vaccination, duck plague vaccine, age, route, ducklings DOI = 10.3329/bjvm.v6i1.1335 Bangl. J. Vet. Med. 2008. 6 (1): 27-30