The isolating performance of NSS (Negative-Stiffness-Structure) on ameliorating the automotive’s comfortable in all vertical, pitching, and rolling directions has been not studied yet. Thus, two vibration models of the automobile including the two-dimensional (2-D) model and three-dimensional (3-D) model are built to evaluate automobile’s comfortable efficiency under various conditions. The reduction of the seat and automobile accelerations has been used as an evaluation index. Results present that seat’s vertical acceleration using NSS is evidently improved compared without NSS in two models of the automobile. Especially, the seat’s root-mean-square acceleration with NSS is strongly deteriorated by 65.1% with 2-D model and reduced by 69.7% with 3-D model in comparison without NSS. However, the seat’s NSS does not affect the pitching vibration of the automobile’s body. Besides, from the comparison results between 2-D and 3-D models, seat’s NSS could strongly ameliorate the comfortable efficiency in both vertical and rolling vibrations while the seat’s NSS does not affect improving the automobile’s pitching angle of the automobile. Therefore, to fully assess NSS’s isolating performance as well as improve the comfortable efficiency of the automobile in all vibration directions, 3-D model of the automobile should be used.