Abstract Twisting is an important part in the production process of large package E-glass yarn. To derive the twisting process parameters of large package E-glass yarn, a method from the physical properties of bobbin yarn is proposed based on the study of the twisting principle of E-glass yarn. After unwinding the bobbin yarn and measuring its twist, loop pitch, and corresponding diameter, the calculation is completed according to the steps of first determining the descending speed of the traveler, then fitting the spindle speed data, and finally calculating the elevating speed of the traveler. The result is that the twisting spindle speed of D450 E-glass yarn with 31.8 T/m decreases from 5,015 to 4,550 rpm, the elevating speed of the traveler is 0.91 m/min, and the descending speed of the traveler is 1.50 m/min. The accuracy of the calculation is verified by monitoring the rotating speed of the roving cake and measuring the loop pitch of bobbin yarn in the twisting experiment. The result shows that the prediction error of this method is less than 1.1%, which can effectively derive the twisting process parameters of large package E-glass yarn, and provide a reference for the twisting process design scheme of glass fiber strand manufacturers.