Pisum Sativum Var Research Articles

Edible-pod Peas as High-value Crops in the U.S. Virgin Islands

Snow pea (Pisum sativum var. saccharatum) and sugar snap pea (P. sativum var. macrocarpon) are high-value crops typically grown in temperate regions. Temperature is the main limiting factor to growing edible-pod peas in warmer U.S. states and territories. The objective was to evaluate edible-pod peas performance in tropical climates and to make cultivar recommendations to farmers in the U.S. Virgin Islands based on fruit yield. Trials were performed in two consecutive years (2014 and 2015), testing six cultivars of edible-pod peas: three snow pea (Little Sweetie, Mammoth Melting, and Oregon Giant) and three sugar snap pea (Super Sugar Snap, Cascadia, and Sugar Sprint) in a complete randomized block with four replications. ‘Little Sweetie’ produced the highest total fruit yield for the season (15,442 kg·ha−1) and ‘Mammoth Melting’ (4249 kg·ha−1) and ‘Sugar Sprint’ (3349 kg·ha−1) produced the lowest total fruit yield in Year 1. The same trend happened in Year 2, where ‘Little Sweetie’ (14,322 kg·ha−1) and ‘Super Sugar Snap’ (12,511 kg·ha−1) were higher yielding and ‘Mammoth Melting’ (4582 kg·ha−1) and ‘Sugar Sprint’ (1929 kg·ha−1) were the lowest yielding cultivars. ‘Mammoth Melting’ showed a marketable yield below 80% of total yield in Years 1 and 2. ‘Mammoth Melting’ and ‘Super Sugar Snap’ produced the tallest plants in Year 1, while ‘Mammoth Melting’ was significantly taller than the others in Year 2. As expected, sugar snap pea presented fruit soluble solids concentration (SSC) 2.7% to 6.5% higher than snow pea. The snow pea cultivars had longer mean fruit length (81 to 86 mm) than sugar snap pea (60 to 68 mm). The opposite trend occurred with fruit thickness; sugar snap pea averaged 28.5% thicker than snow pea. The shoot dry weight of ‘Sugar Sprint’ was on average 78.5% smaller than ‘Mammoth Melting’ and ‘Oregon Giant’, resulting in poor performance due to small plant size. ‘Mammoth Melting’ and ‘Super Sugar Snap’ had the lowest chlorophyll content compared with the other cultivars. Results of this experiment indicate that edible-pod peas have potential as a specialty, short-season, high-value crop when grown in the cool–dry winter months of the U.S. Virgin Islands. Of the cultivars tested, Little Sweetie was the highest yielding cultivar evaluated within the environmental and geographical conditions of this study for two consecutive years.

Eficiencia en la nodulación por rizobios nativos, procedentes de nódulos de Pisum sativum “arveja” colectados de diferentes Departamentos del Perú

In the present investigation was able to authenticate the ability to nodulate and to evaluate the frequency of nodulation efficiency of 50 isolate strains of pea nodules, collected from the departments: La Libertad, Lambayeque, Cajamarca and Lima. Which was inoculated to 1 mL of culture suspension alone (1.2x109 cel /mL) in seedlings of Pisum sativum var. Selection Junin, keeping them under conditions of relative humidity,50-70% and temperature / photoperiod, 28 °C – 24 °C / 14 h – 10 h (light-dark). At 25 days post-inoculation was obtained that 40% nodulated the host plant. To determine the frequency of nodulation efficiency, we took into account the parameters of speed of nodulation (DN in mm) and the percentage of roots nodulated (PRN%); pearson correlational analysis of the 5% significance, MANOVA and Tukey statistical DHS, allowed to establish the ranges (high, medium and low) for DN and PRN% for each crop, resulting in only 10% of all crops (Ra Ra 218-01 and 212-01) showed high efficiency in nodulation, as DN were high and high PRN%.

Winter Annual Legumes Seeded into Bermudagrass: Production, Nutritive Value, and Animal Preference

Core Ideas Overseeding winter annual legumes can extend grazing season and reduce feed costs. Overseeding winter annual legumes into bermudagrass improved subsequent hay quality. Overseeding winter annual legumes can increase nutrient harvest from the soil. Overseeding cool‐season annual legumes into bermudagrass [Cynodon dactylon (L.) Pers.] offers an economical solution to pasture shortage experienced by beef cattle (Bos spp.) farmers during winter on the southeastern Coastal Plain of the USA. Using Austrian winter peas [Pisum sativum var. arvense (L.) Poir.], ‘Dixie’ crimson clover (Trifolium incarnatum L.), ‘Yucchi’ and ‘Apache’ arrowleaf clover (T. vesiculosum Savi), and ‘AU Merit’ hairy vetch (Vicia villosa Roth.) in replicated complete blocks (four replicates in each of two growing seasons), our objectives were to (i) determine the nutritive value of forage provided by several legumes as they mature; (ii) evaluate legume impact on the nutritive value of subsequent bermudagrass hay harvests; (iii) obtain estimates of the quantity of N they potentially add to the system; and (iv) evaluate the preferences of grazing cattle for the various legumes. All legumes improved early‐spring dry matter (DM) yield compared with control plots without overseeding (3310 vs. 1120 lb/acre for legumes and control; P < 0.01). Legumes increased the crude protein (CP) content of bermudagrass hay harvested during the subsequent growing season (14.1, 14.8, 13.7, 14.8, and 12.0% for crimson, vetch, peas, arrowleaf, and control, respectively; P < 0.01); however, peas and arrowleaf reduced hay DM yield (P = 0.04). Across species, legumes added approximately 89 lb/acre N to the system by fixation, and overseeding offered a potential addition of 64 grazing days/acre (1200‐lb cows). Legumes improved the potential removal of both N and P from the system, and cattle readily consumed the legumes. Overseeding may have the potential to reduce N fertilization and lengthen grazing season; however, with added seed costs, it may not improve profitability.

Study of genetic variability, character association, path analysis and selection parameters for heterotic recombinant inbred lines of garden peas (Pisum sativum var. Hortense L.) under mid-hill conditions of Himachal Pradesh, India

The present investigations involving forty five diverse pea recombinant inbreds and three standard checks were carried out for two successive years during 2011-2012 and 2012-13. The objective of the study was to ascertain genetic variability, heritability, genetic advance, correlation and path coefficient among yield and other horticultural and quality traits and identification of superior recombinants for their utilization in crop improvement programme. Wide range of variability was observed for most of the characters under study. The magnitude of phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variability (GCV) for all the traits. High phenotypic and genotypic coefficient of variations were recorded for protein content, ascorbic acid, plant height and pod yield per plant for both the years. Pod yield per plant and plant height exhibited high heritability associated with high genetic advance in the respective years indicating the additive gene action for their expression and are likely to respond better to selection. Pod yield showed a positive and significant correlation with number of pods per plant, pod length and number of seeds per pod both the years. Path analysis revealed that number of pods per plant and pod length have highest positive direct effect both the years and thus it may be treated as selection criteria for isolating higher yielding genotypes in garden peas. In heterosis study, some of the recombinant inbreds were found superior over standard checks which include L-14 and L-13 for pod yield/plant, number of pod/plant and earliness. These findings can be further utilized to develop and enhance the yield potential of garden pea.

Storage quality of shelled green peas under modified atmosphere packaging at different storage conditions.

Storage quality of shelled green peas (Pisum sativum var. sativum L) was investigated under modified atmosphere packaging (MAP: perforated and non perforated) compared to unsealed samples, respectively, at T1 (4±1°C and 94±2% RH) and T2 (10±1°C and 90±2% RH) for each sample and during period of storage (8, 16 and 24days). Modified atmosphere (MA) was created using low density polyethylene (LDPE) film packages having 107μm of film thickness and package size of 0.022 m(2). Quality parameters viz., weight loss (WL), total phenolic content (TPC), instrumental colour, ascorbic acid (AA) and sensory characteristics were evaluated during storage period. Weight loss was in the range of 0.18 to 3.54 (zero perforation at T1), 0.21 to 6.48(unsealed samples at T2) and 0.31 to 9.64% (zero perforation at T1) after 8, 16 and 24days of storage, respectively. Total phenolic content significantly increased to 102.47-161.54mg/100g from an initial value of 91.53mg/100g for all the samples and treatments studied. The MAP non perforated sample stored at T2 recorded maximum Hunter 'L' and '-a' colour values than all other samples. A significant decrease in AA content was observed in all the samples with maximum loss (53.77%) in unsealed sample stored at T2, whereas MAP (3 perforations) sample stored at T1 retained maximum AA (90.50%). Sensory quality analysis revealed that MAP (3 perforations) sample stored at T1 was in acceptable quality, with good appearance and overall acceptance. The study shows that shelled green peas can be stored in MAP with 3 perforations (0.4mm dia) in the temperature range of 4 to 10°C and 90-94% RH to extend shelf life with marketable quality for 24days.

English

Dekoko (Pisum sativum var. abyssinicum) is a unique crop developed and cultivated in Ethiopia. The objectives of the study were; understanding the genetic variability present in Dekoko population, determining the correlation between grain yield, protein content and other traits and understanding traits that can be used for indirect selection for high grain yield and high protein content. Local collections of Dekoko were planted in 3 replications of the RCBD design at Mekhan farmers’ Training Centre in Endamekhoni during 2010.Traits such as days to flowering and maturity and leaf width had low phenotypic coefficient of variation and genotypic coefficient of variation and low genetic advance (<20%). The genotypic correlation between and the direct effect of days to flowering, plant height and biomass on seed yield was positive suggesting that the  traits can be used for indirect selection of high yielding accessions. Seed yield and protein content had perfect negative genotypic correlation (-1.00). The direct effect of protein content on seed yield was also negative implying that simultaneous selection towards increased seed yield and increased protein content at the same time may be difficult.   Key words: Associations, Dekoko, Pisum sativum var. abyssinicum, accession, crude protein.

Evaluation of the quality of protein in cotyledons and radicles of selected legumes after germinating as a healthy food

The quality of protein in cotyledons and radicles (shoot) was separately investigated in selected legumes, namely: Pisum sativum (cultivars: Terno, Xantos, Svit, Achat), Glycine max, Lupinus albus (cultivar Amiga), Pisum sativum var. arvense (cultivar Arkta), Faba vulgaris (cultivar Piestansky) after germinating for 48 hours and compared with the respective raw seeds. The highest content of amino acid in cotyledons and radicles were noted in P. sativum (Xantos) and its phenylalanine was the highest increased essential amino acid in radicles 7.4 g/16 g N with respective raw seeds 4.6 g/16 g N. The in vitro protein digestibility of cotyledons and radicles increased significantly (P < 0.05) of all legumes under study. It is ranged from 79.1% to 86.4% in cotyledons, 86.7% to 93.4%. in radicles and 54.1% to 75.0% in respective raw seeds. The quality of proteins in cotyledons and radicles of all legumes was improved significantly after germinating 48 hours.

Screening for powdery mildew (Erysiphe pisi D.C.) resistance gene-linked SCAR and SSR markers in five breeding lines of Pisum sativum L.

SummaryA major disease in garden pea (Pisum sativum var. hortense) is powdery mildew caused by Erysiphe pisi. Homozygous recessive alleles of the er1 and er2 genes, and the dominant allele of Er3 have been found to contribute to powdery mildew resistance in pea. Nine parental genotypes used for pea breeding in India were screened using 12 sequence characterised amplified region (SCAR) markers and five simple sequence repeat (SSR) markers. None of the SCAR markers could differentiate between the resistant or susceptible genotypes, although they amplified at the specific loci previously reported. The SSR marker A5 clearly distinguished the homozygous resistant and susceptible parents and three F2 progeny from crosses of ‘Arka Priya’ × 'IP-3’, ‘Arka Pramod’ × ‘IP-3’, and ‘Arka Ajit’ בAzad-Pea’. Chisquare analysis showed a 1:3 phenotypic segregation pattern and a genotypic ratio of 1:2:1 for the SSR marker A5. Since SSR A5 is a co-dominant marker, it distinguished the clear homozygous (Er1Er1/er1er1) and heterozygous Er1er1) allelic conditions of er1 at the F2 progeny level. The SSR marker A5 can therefore be used for marker assisted breeding for powdery mildew resistance in pea.

Variability and character association studies in garden pea (Pisum sativum var. hortenseL.) during winter season at mid hills of Himachal Pradesh

Fifty four genotypes including four checks were grown in a RBD with three replications during Rabi 2010-11 at Vegetable Experimental Research Farm, Nauni, Solan HP to estimate the parameters of variability and association of important characters with yield in garden pea. Analysis of variance showed significant differences among the genotypes for all the morphological characters under study. The genotypic and phenotypic coefficients of variation were high for total soluble solids, total sugars, pod yield per hectare and total phenols. High heritability estimates coupled with high to moderate genetic gain observed for pod yield (kg/plot), node at which the first flower appear (number), number of pods per plant and total Phenols (g/100g). Pod yield was positively correlated with number of pods per plant, pod length (cm), number of seeds per pod and shelling percentage thereby indicating that the selection based on these traits could be effective for improvement of green shelled peas yield.

Occurrence and Characterization of Bean common mosaic virus Strain NL1 in Iowa.

A legume survey in Story County was conducted to characterize legume viruses present in Iowa and potentially problematic to soybeans. Symptomatic (chlorosis, leaf curl, or vein banding) and non-symptomic leaves were selected from Apios americana, Cyamopsis tetragonolobus, Desmodium spp., Glycine max, Lablab purpureus, Lotus japonicus, Phaseolus coccineus, Phaseolus vulgaris, Vicia faba, Vigna ungulculata, and Chenopodium quinoa (an indicator species), and sent to Agdia Inc. for their Crop Screen: "Bean (Soybean)" tests. Cowpea mosaic virus (in L. purpureus), Southern bean mosaic virus (in A. americana), Soybean dwarf virus (in L. purpureus), and Tobacco streak virus (in Desmodium spp.) were identified. In addition, P. vulgaris was found positive in the serological Potyvirus group test, and was further characterized using reverse transcription (RT)-PCR using total RNA (Qiagen RNeasy extraction kit) from symptomatic leaves. A ~1-kb fragment of virus genome was amplified using degenerate primers, forward: 5' TGYGTNGAYGAYYTYAAYAA 3' (3) and reverse: 5' TCRTARAARTCRAAIGCRTAICKIG 3' (2). The closest GenBank BLAST hits for the sequence were BCMV strain NL1 (GenBank Accession No. AY112735) and BCMV MS1 (EU761198), both at 97% identity. RNA sequencing using an Illumina HiSeq 2500 (100 base paired ends) allowed construction of a nearly complete viral sequence from positions 38 to 10,037 nt (KM023744). This showed 99% similarity to BCMV-NL1 (AY112735) and 98% similarity to BCMV MS1 (EU761198). The BCMV isolate was recovered on the P. vulgaris cv. Taos Red and tested against a panel of Phaseolus, Glycine, and other hosts. Symptomatic hosts (vein-banding, mosaic, stunting, and leaf curl) were P. vulgaris cvs. Starlight, Tohono O'odham vaya Amarillo, Black Valentine Stringless, Sutter Pink, Hidatsa red, Stringless Green Refugee, Burpees Stringless Improved Bush, Princess d'Artois, Princess Double de Holland, and Dubelle Witte. Asymptomatic hosts included P. vulgaris cvs. Sierra, Great Northern Tara, Tara, and Vigna radiata. Non-host plants were P. vulgaris cvs. Michelite 62, Great Northern UI 31, Great Northern UI 123, Pure Gold Wax, Top Crop, Redlands Greanleaf B, Red Mexican UI 34, Imuna, Monroe, Pinto UI 114, Widusa, Othello, Sacramento, Red Kidney, Provider, Black Turtle Soup, Victor, and Witte boon, G. max cv. Williams, Cucumis sativus cv. Straight 8, Solanum lycopericum cv. Reine des Hatives, Vicia faba, Pisum sativum var. macrocarpon, V. unguiculata, and A. americana. The host range of the BCMV isolate on common bean differential species concurred with pathogenicity group I (1) and agreed with assignment as strain NL1. To our knowledge, this is the first complete characterization of a pathotype I strain NL1 of BCMV in the United States, combining biological typing and whole genome sequencing.

Legume Seeds and Rapeseed Press Cake as Replacers of Soybean Meal in Feed for Fattening Pigs

Abstract The possibility of replacing soybean meal with a mixture of legume seeds and rapeseed press cake (RPC) was evaluated on 60 pigs weighing about 30 kg at the beginning of the experiment. Pigs were allocated to 5 experimental groups. Group I (control) received standard feed mixture containing soybean meal as a main protein source. Next groups received rapeseed press cake (RPC) mixed with fodder pea (Pisum sativum var. Ramrod) - group II, field bean (Vicia faba var. Kasztelan) - group III, blue lupin (Lupinus angustifolius var. Regent) - group IV or yellow lupin (Lupinus luteus var. Mister) - group V. Soybean protein was replaced by experimental protein sources at about 30% in grower (17% legumes, 13% rapeseed press cake) and at 100% in finisher diets (experimental proteins in equal ratio accounted for about 55% of mixture protein). Limited feeding was used, water was available ad libitum. Half the animals in each group received mixtures supplemented with fibrolytic enzymes Ronozyme VP and Ronozyme WX. Apparent digestibility of feed nutrients was estimated using the balance method on 30 fatteners not used in the fattening experiment, weighing about 40 kg for grower and 70 kg for finisher diets. Gross composition of legume seeds and RPC, amino acid composition of their protein, glucosinolate content in RPC and tannin content in faba bean and alkaloids in lupins were analysed. Carcass traits and meat quality were also estimated. Legume protein content ranged from 19.6% (pea) to 39.8% (yellow lupin). RPC protein contained more sulphur amino acids than legume protein. There was no significant difference in protein and fat digestibility. Body weight gains of fatteners fed with blue lupin were comparable to controls but significantly lower than those of the remaining groups. Supplemental enzymes improved body weight gains of fatteners receiving field bean. There was no significant difference in carcass traits and meat quality except for sensory analysis. It is concluded that the mixture of RPC and legume seeds can replace soybean meal in fattener feed.

Perfluoroalkyl acid distribution in various plant compartments of edible crops grown in biosolids-amended soils.

Crop uptake of perfluoroalkyl acids (PFAAs) from biosolids-amended soil has been identified as a potential pathway for PFAA entry into the terrestrial food chain. This study compared the uptake of PFAAs in greenhouse-grown radish (Raphanus sativus), celery (Apium graveolens var. dulce), tomato (Lycopersicon lycopersicum), and sugar snap pea (Pisum sativum var. macrocarpon) from an industrially impacted biosolids-amended soil, a municipal biosolids-amended soil, and a control soil. Individual concentrations of PFAAs, on a dry weight basis, in mature, edible portions of crops grown in soil amended with PFAA industrially impacted biosolids were highest for perfluorooctanoate (PFOA; 67 ng/g) in radish root, perfluorobutanoate (PFBA; 232 ng/g) in celery shoot, and PFBA (150 ng/g) in pea fruit. Comparatively, PFAA concentrations in edible compartments of crops grown in the municipal biosolids-amended soil and in the control soil were less than 25 ng/g. Bioaccumulation factors (BAFs) were calculated for the root, shoot, and fruit compartments (as applicable) of all crops grown in the industrially impacted soil. BAFs were highest for PFBA in the shoots of all crops, as well as in the fruit compartment of pea. Root-soil concentration factors (RCFs) for tomato and pea were independent of PFAA chain length, while radish and celery RCFs showed a slight decrease with increasing chain length. Shoot-soil concentration factors (SCFs) for all crops showed a decrease with increasing chain length (0.11 to 0.36 log decrease per CF2 group). The biggest decrease (0.54-0.58 log decrease per CF2 group) was seen in fruit-soil concentration factors (FCFs). Crop anatomy and PFAA properties were utilized to explain data trends. In general, fruit crops were found to accumulate fewer long-chain PFAAs than shoot or root crops presumably due to an increasing number of biological barriers as the contaminant is transported throughout the plant (roots to shoots to fruits). These data were incorporated into a preliminary conceptual framework for PFAA accumulation in edible crops. In addition, these data suggest that edible crops grown in soils conventionally amended for nutrients with biosolids (that are not impacted by PFAA industries) are unlikely a significant source of long-chain PFAA exposure to humans.

English

High salt level of a germination medium may induce a reduction, delay and even complete inhibition of germination due to osmotic effect and/or ion toxicity. The objective of this study was to investigate the effect of salinity due to NaCl on germination and early seedling growth of two crops, Pisum sativum var. abyssinicum and Lathyrus sativus. Seeds of these two crops were treated with NaCl induced saline germinating media prepared in Petri dishes. Fifty (50) surface sterilized seeds per Petri dish were sown in five salt treatments (0, 5, 7, 9 and 15 dSm-). Each treatment was replicated four times. Germination percentage, shoot length and root length of both crops decreased with an increase in salinity level. Although both crops are low salt tolerant legumes, P. sativum var. abyssinium was found to be less tolerant than L. sativus. This study could be strengthened by further work under field conditions and also at mature vegetative and reproductive stages of the crops. Key words: Germination time, water uptake percentage, salt tolerance, seedling biomass.

Wheat (Triticum aestivum) productivity under different tillage practices and legume options in rice (Oryza sativa) and wheat cropping sequence

Screening of suitable tillage practices and legumes between rice (Oryza sativa L.) and wheat (Triticum aestivum L.) is the need of hour to maintain the wheat productivity, which is hampering in rice wheat sequence. Considering this, experiments were conducted during 2006-07 to 2009-10, at Norman E Borlogue Crop Research Centre of G B Pant University of Agriculture &amp; Technology, Pantnagar. The experimental plots were silty clay loam, medium in organic carbon, available phosphorus and low in nitrogen, however the pH was slightly alkaline. Pooled analyses were done for four years considering the years as main plot of split plot design. However, sub plot contained three tillage practices, i.e zero tillage (ZT), raised bed system (RBS) and conventional tillage (CT). Three legumes namely greengram (Vigna radiata L.), cowpea [Vigna unguiculata (L.) Walp.] and vegetable pea (Pisum sativum var hortense) were kept in sub sub plots. The findings revealed that, over the years, wheat yield markedly influenced by maximum temperature as compared to other weather attributes. Simultaneously, tillage and legume interaction was non-significant on wheat yield. Mean effect of the treatments vindicated that metric parameters, viz plant height, dry matter accumulation, biological yield, grain yield, straw yield, grain straw ratio and harvest index were significantly influenced by years, tillage practices and legume inclusion. Year 2007-08 was the good year for wheat performance. Among the tillage practices conventional tillage retained maximum metric traits than the other tillage practices, however in case of legume inclusion; cowpea gave maximum metric traits. Significant effects of treatments were also noticed on NPK uptake, available N in soil, net returns and benefit cost ratio and system productivity. Maximum values of these parameters were noticed during 2007-08. Maximum available N, NPK uptake were observed in zero tillage. Greengram containing plots had maximum available N but vegetable containing plots had higher nutrient content and uptake. Net return, benefit cost ratio were maximum under zero tillage and vegetable pea containing plots. Conventional tillage and vegetable pea contained plots gave maximum system productivity. Therefore, conventional tillage and cowpea inclusion is good for wheat yield but for economics point of view zero tillage practices and inclusion of vegetable pea is better.

10.5937/ratpov51-5072 = Significance of genetic resources of cool season annual legumes: II: Neglected and underutilised crops

Plant genetic resources are a live treasury of both one country and the whole mankind, although their ex situ preservation and in situ conservation are very demanding in numerous ways, especially if human resources and financial issues are considered. Legumes (Fabaceae Lindl.) are facing the bottlenecks caused by breeding emphasizing yield and quality, raising the questions how to solve the issue of this narrowing the genetic basis of cultivated legume species. The reintroduction of neglected and underutilised crops, such as red vetchling (Lathyrus cicera), Cyprus vetchling (Lathyrus ochrus), French serradella (Ornithopus sativus), Ethiopian pea (Pisum abyssinicum), field pea (Pisum sativum var. arvense), fenugreek (Trigonella phoenum-graecum), bitter vetch (Vicia ervilia), Narbonne vetch (Vicia narbonensis), Hungarian vetch (Vicia pannonica) and hairy vetch (Vicia villosa) may significantly assist many contemporary farming systems by diversifying their needs and improve the efficiency of environment resources.

Angiotensin I converting enzyme inhibitory peptides obtained after in vitro hydrolysis of pea (Pisum sativum var. Bajka) globulins.

Pea seeds represent a valuable source of active compounds that may positively influence health. In this study, the pea globulins were digested in vitro under gastrointestinal condition and potentially bioaccessible angiotensin I converting enzyme (ACE) inhibitory peptides were identified. The degree of hydrolysis after pepsin, 14.42%, and pancreatin, 30.65%, were noted. The peptides with the highest ACE inhibitory properties were separated using ion exchange chromatography on DEAE-cellulose. Thirteen peptides fractions were obtained but only four showed potential antihypertensive properties. The highest inhibitory activity was determined for the fraction F8 (IC50 = 0.0014 mg/mL). This fraction was separated on Sephadex G10 and two peptide fractions were obtained. The peptides fraction (B) with the highest ACE inhibitory activity (IC50 = 0.073 mg/mL) was identified by ESI-MS/MS. The sequences of ACE inhibitory peptides were GGSGNY, DLKLP, GSSDNR, MRDLK, and HNTPSR. Based on Lineweaver-Burk plots for the fraction B, the kinetic parameters as K m, Vmax, and K i and mode of inhibition were determined. This fraction belongs to uncompetitive inhibitor of ACE activity. The seeds of pea are the source of precursor protein, which releases the ACE inhibitory peptides as a result of enzymatic hydrolysis.

Effect of planting pattern (row spacing and seed spacing in row) on some pea physical properties (Pisum sativum var. Pofaki)

In this study, peas were planted according to the map. Product management was done completely from planting to mature period and harvest time. Plant to plant distance in row, was determined 5, 7 and 9 cm and row distance was selected 30, 40 and 50 cm. For measuring pea dimensions, 15 peas were selected randomly from pods. All process was repeated three times for all levels and data were analyzed by using of completely randomized design (CRD) and SAS software. The effect of seed spacing in row was significant on length, width, geometric diameter, arithmetic diameter, cross sectional area, mass, actual volume and bulk volume at the level of 5% (P<5%) and thickness at the level of 1% (P<1%). But it was not significant on lateral surface and sphericity ratio. Also the effect of row distance and interaction effect of seed spacing in row and row distance were not significant in any case.

English

Cultivated&nbsp;Pisum&nbsp;is dominated with&nbsp;Pisum sativum&nbsp;subgroup, but&nbsp;P. sativum&nbsp;var.abyssinicum&nbsp;(Dekoko) is a unique subgroup developed and cultivated in Ethiopia. The objectives of the study were the characterization of the accessions using three qualitative traits and understanding the genetic diversity of the crop. Twenty-four (24) accession/local collections of Dekoko collected from South Tigray and North Wello by Alamata Agricultural Research Centre were planted in three replications of the Randomized Complete Block Design (RCBD) at Mekhan farmers&rsquo; Training Centre in Endamekhoni in 2010. Characterization of the accessions by three qualitative traits viz., flower color, seed size and seed shape had revealed the existence of high genetic diversity of the population (H&rsquo;=0.84, 0.95, 0.98, respectively, with mean H&rsquo;=0.92) implying that the crop can be improved through breeding. The distribution of the various categories of the three qualitative traits (flower color, seed shape and seed size) was independent of region and altitude. Clustering of the accessions by qualitative traits produced seven distinct clusters which did not classify the accessions according to regions or Weredas, but according to three altitude classes. &nbsp; &nbsp; Key words:&nbsp;Characterization, Dekoko,&nbsp;Pisum sativum&nbsp;var. abyssinicum, accession/collection.

Combining ability and gene action studies for horticultural traits in garden pea: A review

Identification of genetically superior parents is an important prerequisite in garden pea for the development of elite strains. The combining ability analysis has been the most important and efficient tool in choosing the desirable parents for hybridization programmes. The attraction of combining ability is that this provides an empirical summary of complex observations and a reasonable basis for forecasting the performance of yet untested crosses but yet makes no assumptions genetically. Being based on first degree statistics (totals, means), they are statistically robust and, being genetically, so to speak, neutral, they are equally applicable to in breeder and out breeder, whether seed propagated or clonal. This technique makes it possible to classify the parental lines in terms of superiority in hybrid combinations and the gene action involved in the inheritance of different characters. Therefore, analysis of combining ability has been the most important and efficient tool in selecting the desirable parents for a hybridization programme. This review will be help in understanding of the exciting opportunities offered by combining ability and gene action studies in graden pea (Pisum sativum var. hortense L.). Key words: Combining ability, garden pea, gene action, horticultural traits.

FT-IR 스펙트럼 기반 다변량통계분석기법에 의한 두과작물의 대사체 수준 식별체계 확립

본 연구에서는 국내에서 재배중인 대표적인 두과작물(대두, 완두, 강낭콩, 팥, 녹두, 동부)종자로부터 전세포추출물의 FT-IR 스펙트럼 데이터로부터 다변량통계분석(PCA, PLS-DA, HCA)을 이용하여 신속하고 간편한 종 구분체계를 확립하였다. 대사체수준에서 팥, 녹두, 동부는 유연관계가 높음을 알 수 있었으며 대두, 완두, 강낭콩은 비록 두과작물이지만 차이가 매우 큼을 알 수 있었다. 아울러 본 연구에서 얻어진 대사체 정보의 다변량통계분석에 의한 유연관계분석은 흥미롭게도 두과작물의 계통분류학적 유연관계와 밀접한 상관관계를 나타내었다. 따라서 FT-IR 스펙트럼 데이터의 다변량통계분석은 방법의 간편성과 신속성을 고려할 때 두과작물의 계통이나 품종의 신속한 식별 수단으로 활용이 가능할 것으로 기대된다. 또한 두과작물의 기능성 성분 함량 정보가 성공적으로 연계된다면 본 연구에서 확립된 대사체 기반 신속식별체계는 기능성 성분의 함량이 높은 계통이나 품종의 조기 선발수단으로 활용이 가능할 것으로 기대된다. To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.