High compression ratio natural gas engine (NGE) is prone to knock at high load, which aggravates the thermal load of piston. Therefore, accurately evaluating the piston thermal load in the practical design is essential to enhance engine reliability and improve performance. The typical piston thermal load simulation adopts uniform wall boundary conditions, making it difficult to reveal the influence of turbulent flame and knock on the piston thermal load. In this paper, a stepwise decoupling method is applied to calculate the piston thermal load. The advancement of this method lies in its integration of chemical reaction mechanism with computational fluid dynamics (CFD) to calculate the spatial variations in heat transfer coefficient (HTC) and near-wall gas temperature at the piston top. Moreover, the conjugate heat transfer (CHT) method is adopted to solve the piston thermal load by coupling the thermal boundary conditions (TBC) obtained in the first step with the cooling oil boundary conditions. Based on this method, the effect of knock on piston thermal load of a high compression ratio NGE under different knock intensities is explored in this paper. It is found that turbulent combustion causes uneven distribution of thermal load at piston top. The decrease in knock intensity significantly reduces the gas temperature, heat flux and thermal load at piston top. Particularly under severe knock, the average temperature of piston is 22.9 K and 32.4 K higher than that of light knock and normal combustion, respectively. Furthermore, the main factor affecting the piston temperature field distribution is the gas temperature rather than the HTC. The gas temperature at piston top is overall the highest on the side near the inlet valve pit, which leads to a higher temperature on this side. Compared to light knock and normal combustion, when severe knock occurs, the thermal load on the exhaust valve pit increases significantly. This study provides an effective method for analyzing the heat transfer of the piston under knocking combustion, which is of guiding significance for controlling the thermal load of the high-temperature components in the combustion chamber.