Extract Of Piper Sarmentosum Research Articles

Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide

Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H(2)O(2))-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H(2)O(2) (0-1000 micromol/L) and it was observed that 180 micromol/L H(2)O(2) reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H(2)O(2)-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 microg/ml) of three different extracts (aqueous, methanol and hexane) of PS. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H(2)O(2)-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H(2)O(2)-induced HUVECs.

Piper Sarmentosum Increases Nitric Oxide Production in Oxidative Stress: A Study on Human Umbilical Vein Endothelial Cells

OBJECTIVE:Nitric oxide produced by endothelial nitric oxide synthase (eNOS) possesses multiple anti-atherosclerotic properties. Hence, enhanced expression of eNOS and increased Nitric oxide levels may protect against the development of atherosclerosis. Piper sarmentosum is a tropical plant with antioxidant and anti-inflammatory activities. This study aimed to investigate the effects of Piper sarmentosum on the eNOS and Nitric oxide pathway in cultured human umbilical vein endothelial cells (HUVECs).METHODS:HUVECs were divided into four groups: control, treatment with 180 μM hydrogen peroxide (H2O2), treatment with 150 μg/mL aqueous extract of Piper sarmentosum, and concomitant treatment with aqueous extract of PS and H2O2 for 24 hours. Subsequently, HUVECs were harvested and eNOS mRNA expression was determined using qPCR. The eNOS protein level was measured using ELISA, and the eNOS activity and Nitric oxide level were determined by the Griess reaction.RESULTS:Human umbilical vein endothelial cells treated with aqueous extract of Piper sarmentosum showed a marked induction of Nitric oxide. Treatment with PS also resulted in increased eNOS mRNA expression, eNOS protein level and eNOS activity in HUVECs.CONCLUSION:Aqueous extract of Piper sarmentosum may improve endothelial function by promoting NO production in HUVECs.

In vivo anti-nociceptive and anti-inflammatory activities of the aqueous extract of the leaves of Piper sarmentosum

Ethnopharmacological relevance Piper sarmentosum (Piperaceae) is a medicinal plant traditionally used by the Malays to treat headaches, toothaches, coughs, asthma and fever. Aim of the study In order to establish the pharmacological properties of the leaf of this plant, studies were performed on anti-nociceptive and anti-inflammatory activities. Materials and methods The aqueous extract of Piper sarmentosum (AEPS) was prepared in the doses of 30, 100 and 300 mg/kg. Anti-nociceptive activity of AEPS was evaluated by abdominal constriction and hot-plate tests. AEPS was also pre-challenged with 5 mg/kg naloxone to determine the involvement of opioid receptors. Anti-inflammatory activity was evaluated using carrageenan-induced paw edema assay. Results Subcutaneous administration of AEPS exhibited anti-nociceptive activity ( P < 0.05) in a dose-dependent manner in the abdominal constriction and hot-plate tests. Pre-treatment with naloxone completely ( P < 0.05) diminished the extract anti-nociceptive activity in both tests. The AEPS, at all doses used, exerted significant ( P < 0.05) anti-inflammatory activity in a dose-dependent manner. Conclusions The AEPS exhibits opioid-mediated anti-nociceptive activity at the peripheral and central levels, as well as anti-inflammatory activity, which confirmed the traditional uses of the plant in the treatment of pain- and inflammatory-related ailments.

Neuromuscular blocking activity of methanolic extract of Piper sarmentosum leaves in the rat phrenic nerve-hemidiaphragm preparation

Methanolic extract of Piper sarmentosum Roxb. (Piperaceae) leaves was studied for the neuromuscular blocking activity in rat phrenic nerve-hemidiaphragm preparations. The plant extract, at concentrations of 3.2, 4.0, 4.8 and 6.4 mg/ml, exhibited an initially transient increase in twitch tension which was followed by a marked dose-related neurally-evoked twitch depression. The neuromuscular blocking effect produced by the plant extract was compared with d-tubocurarine (dTC) and succinylcholine (SCh). The EC 50 for neurally-evoked twitch depression of the extract, dTC and SCh was 4.07 mg/ml, 1.1 μm and 15 μm, respectively. The neurally-evoked twitch depression produced by the extract was partially antagonized by tetraethylammonium (TEA) but not by neostigmine (NS). These findings suggested that the plant extract possessed a marked neuromuscular blocking activity at the neuromuscular junction and a possible mechanism which was likely to inhibit neurotransmitter (acetylcholine) release at the presynaptic terminal.

Hypoglycemic effect of the water extract of Piper sarmentosum in rats

The hypoglycemic effect of the water extract of the whole plant of Piper sarmentosum Roxb. (Piperaceae, Thai name: Chaplu) was examined in normal and streptozotocin-diabetic rats. In an oral glucose tolerance test, a single oral administration of the water extract at doses of 0.125 and 0.25 g/kg significantly lowered the plasma glucose level in the normal rats. A reference drug, glibenclamide, at a dose of 5 mg/kg (per os, p.o.) also showed a significant hypoglycemic effect in the normal rats. In contrast, a single oral administration of the water extract at these doses and glibenclamide did not significantly lower the plasma glucose level in the diabetic rats. However, the repeated oral administration of the water extract at a dose of 0.125 g/kg for 7 days produced a significant hypoglycemic effect in the diabetic rats. Glibenclamide (5 mg/kg, p.o.) also caused significant hypoglycemia in the diabetic rats. These results demonstrated that the water extract of whole plant of Piper sarmentosum has a hypoglycemic effect in rats.