Betel Research Articles

Study on Sensory Evaluation and the Optimum Levels of Calcutta Betel Vine (Piper betel) Leaves Extract in the Preparation of Shrikhand

The present investigation was conducted at Section of Animal Husbandry and Dairy Science, College of Agriculture, Nagpur (Dr. PDKV, Akola) during 2022-2023. An effort was made to determine the optimum level of Calcutta betel vine leaves extract in the preparation of shrikhand, with an objective to evaluate the product by sensory quality along with its cost structure and physio-chemical properties. As per current market scenario, the limited variety of shrikhand is available in the stores but this study might help in addition to available varieties in the market. The present study was carried out with five treatments including control T1 and shrikhand prepared from cow milk chakka with different levels of Calcutta betel vine leaves extract i.e. 2.0%, 4.0%, 6.0% and 8.0% in treatment T2, T3, T4 and T5, respectively. The results showed that, the mean score of flavour of shrikhand in treatment T4 was highest (8.5) and lowest was recorded in T5 (5.0). The mean score for body and texture of shrikhand was highest in T4 (8.5) and lowest in T5 (5.2). In terms of colour and appearances, the highest score was recorded in T4 (8.5) and lowest in T5 (5.5). The highest overall acceptability score was recorded in T4 (8.5) over other treatments This superiority was found due to the addition of 6.0% betel vine leaves extract in Shrikhand. It is concluded that, shrikhand of acceptable quality can be prepared by adding 6.0% Calcutta betel vine leaves extract.

Coaxial electrospun PVA-SAP functional nanofibers embedded with betel leaf extract for enhanced germicidal activity and breathability

This study investigates the formation of coaxial electrospun nanofibrous membrane with enhanced germicidal activity and breathability using superabsorbent polymer and polyvinyl alcohol integrated with betel leaf extract and its utilization for developing performance textiles. The functional nanofibrous membrane was fabricated through the coaxial electrospinning technique and characterized for the intended applications. The morphological investigation of the developed nano-membrane using scanning electron microscopy revealed the formation of regular nanofibers with diameters ranging from 154 to 200 nm. The FTIR spectra suggested the existence of functional compounds in the nanofibers from their characteristic peaks. The presence of the bioactive compounds in the ethanolic extract of betel leaf was explored using phytochemical screening. The analysis of antibacterial activity against S. aureus and E.coli using the agar disc diffusion method showed an enhanced zone of inhibition with a maximum dimension of 27 mm and 24 mm against S. aureus and E.coli, respectively. The developed coaxial nanofibrous membranes also demonstrated acceptable particle barrier performance with greater than 95 % efficiency and a superior tensile strength of 99.5 N. Besides, the improved moisture management and air permeability of coaxial nano-membranes embedded with betel leaf extracts suggested an easy transfer of fluid and moisture vapor from the body to the environment via the membrane. Hence, the improved germicidal activity with acceptable particle filtration efficiency and enhanced breathability of the developed coaxial electrospun nano-membranes indicated their potentiality of utilizing them in designing performance textiles with simultaneous barrier performance and breathability for comfort.

In Vitro evaluation of anti-urolithiatic activity of Piper betle L.

Urolithiasis or kidney stone is the most frequently occurring urinary disorders seen all over the world. They are saturated crystals formed due to the minerals present in the urine. It majorly comprises calcium oxalate, uric acid and phosphate crystals. The phosphate crystals, known as struvite, are formed due to the urinary tract infections. Although there are various synthetic medications, the use of traditional medicines / plants has been a promising method to treat kidney stones. To investigate the antiurolithiatic potential of traditional plant - Piper betle, the aqueous extract of Piper betle leaves was used. This extract was tested on two different crystals, first onto the raphides present in Colocasia esculenta leaf which is analogous to the calcium oxalate crystals. Second on the struvite crystals prepared from the crystallization method. The study showed there was a considerable decrease in the amount of raphides present in Colocasia esculenta and the same effect was seen on struvite crystals, which reveals that the leaves of Piper betle have an effect in dissolving and/or minimising the size of both crystals, thus substantiating the traditional claim. It is concluded that the leaves of Piper betle have beneficial inhibitory effect on in vitro crystallization of calcium oxalate and struvite crystals.

IJCM_150A: A profile of Head and Neck Cancer patients attending a tertiary cancer care centre in Southern Karnataka

Background: Overall, 57.5% of head and neck cancers (HNC) globally are from Asia. In India, HNC accounted for 30% of all cancers in males and 11 to 16% in females, of all sites of cancer. HNC in the low- and middle-income countries differ from those in the Western part of the globe in terms of age, site of disease, etiology, and molecular biology. Poverty, illiteracy, advanced stage at presentation, lack of access to affordable treatment facilities pose a major challenge in management of these cancers. Objective: To identify the socio-demographic and epidemiological factors influencing the cause of Head and neck cancer Methodology: A cross sectional study was conducted between February 2023 till November 2023, in Manipal Comprehensive Cancer Care Centre, Kasturba Hospital, Manipal. All the newly diagnosed Head and Neck cancer (HNC) patients aged above 18 years, from December 2022 were included in the study. The participants were interviewed with the structured questionnaire. Details of the socio-demographic characteristics, various risk factors of HNC, presenting symptoms, co-morbid conditions, anthropometric details, dietary habits, level of physical activity when they were healthy, tobacco, alcohol and betel quid use were taken. Institutional Ethics Committee Approval and informed consent from the participants were taken before the initiation of the study Results: The study found that 84% of the head and neck cancer patients were males. 78% of the cases had education only up to or less than 10th std. The middle class and lower class (according to BG prasad scale) constituted 62% of the cases. 70% reported the site of cancer as oral cavity. Conclusion: The study found that consumption of tobacco, alcohol andNon-vegetarian diet are more commonly seen in HNC cases. Also, males, age group of 51-70 years, belonging to middle-class SES, are more affected.

Multi-target mechanism of polyherbal extract to treat diabetic foot ulcer based on network pharmacology and molecular docking

Introduction: Diabetic foot ulcer (DFU) potentially leads to loss of function, infections, hospitalization, lower-extremity amputation, and even death. The potential therapeutic efficacy of a polyherbal candidate named TIP-Heal was identified for treating DFU. TIP-Heal, which stands for Tinospora crispa, Isotoma longiflora, and Piper betle L var nigra, consists of extracts from these three herbs in a ratio of 2:1:1. The Indonesian population commonly uses these herbs due to their wound-healing properties. It is our interest to analyse the mechanism of the polyherbal extract using network pharmacology and molecular docking. Methods: This study uses network pharmacology and molecular docking methods to analyze the multi-target mechanism of active compounds in TIP-Heal extract for DFU treatment. The proteins targeted by the bioactive chemical present in TIP-Heal and DFU were identified within a particular dataset with the keyword "homo sapiens." The identified target proteins were assessed using gene ontology (GO) analysis, the Kyoto Encyclopaedia of Gene and Genomes (KEGG) pathways, protein-protein interactions (PPIs), and molecular docking. Results: The critical proteins obtained were AKT serine/threonine kinase 1 (AKT1), caspase-3 (CASP3), epidermal growth factor receptor (EGFR), proto-oncogene tyrosine-protein kinase Src (SRC) and matrix metalloproteinase-9 (MMP-9). Several compounds, namely PubChem (Compound Identifier=CID: 5319898), 3-epiursolic acid, palmitic acid, and alpha-linolenic acid showed great potential as viable candidates to facilitate the healing process of DFU. Conclusion: The findings of this study indicate that the TIP-Heal extract has the potential to be used as a natural herbal treatment for DFUs with the involvement of AKT1, CASP3, EFGR, and SRC proteins.

Awareness of Oral Submucous Fibrosis among the Quid-Chewing South-Asian Expatriates in the United Arab Emirates.

Oral submucous fibrosis (OSF) is a chronic disorder prevalent in South and Southeast Asia and is mainly related to the habit of chewing betel quid. Although there are numerous South-Asian studies about OSF, there is no study that evaluates the awareness of OSF among expatriate populations of South-Asian origin. Evaluated the awareness of OSF among South-Asian expatriate patients reporting to a dental hospital in Sharjah. Prevalidated questionnaires were given to 150 expatriate patients of South-Asian origin with quid-chewing habit reporting to the teaching clinics of a dental hospital in the United Arab Emirates. Among the 150 patients, 103 responded to the questionnaire. Among the 103 respondents, 11.65% were aware of OSF. Respondents living in shared residency and labor camps had significantly (P = 0.43) lower awareness of OSF compared to respondents living with their families. The results of our study show that the awareness of OSF is lower among the respondents living in labor camps and shared residencies. The younger respondents had more awareness of OSMF and were more likely to quit the quid-chewing habit.

Inhibitory and anti-adherent effects of Piper betle L. leaf extract against Acanthamoeba triangularis in co-infection with Staphylococcus aureus and Pseudomonas aeruginosa: A sustainable one-health approach.

Keratitis is a serious ocular infection often caused by pathogenic microorganisms such as Acanthamoeba spp. Among other harmful microbes, Acanthamoeba keratitis presents a particular challenge due to its resistance to conventional antimicrobial agents. Piper betle Linn., commonly known as betel leaf, has been traditionally used for its medicinal properties. This study aimed to assess the potential of the leaf ethanol extract of P. betle Linn. in the treatment of Acanthamoeba triangularis in monoculture and co-culture with two prevalent pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, associated with keratitis. Minimum inhibitory concentrations (MICs) of A. triangularis, S. aureus, and P. aeruginosa extracts in monoculture and coinfected conditions were examined. In addition, this study explored the potential of the extract in preventing Acanthamoeba adherence in both monoculture and co-culture environments. Scanning electron microscopy (SEM) analysis confirmed the impact of the extract on Acanthamoeba cell membranes, including acanthopodia. Furthermore, a time-kill kinetic assay was used to validate the amoebicidal activity of the extract against A. triangularis and the tested bacteria. MICs for trophozoites, cysts, P. aeruginosa, and S. aureus in the monoculture were 0.25, 0.25, 0.51, and 0.128 mg/mL, respectively, whereas the MICs for Acanthamoeba coinfected with bacteria were higher than those in the monoculture. This extract inhibited the growth of A. triangularis trophozoites and cysts for up to 72 h. Moreover, P. betle extract effectively prevented the adherence of Acanthamoeba to contact lenses under monoculture conditions. SEM analysis confirmed that P. betle extract affects the cell membrane of Acanthamoeba, including Acanthopodia. In addition, the time-kill kinetic assay confirmed that the extract contained amoebicidal activity against A. triangularis, including the tested bacteria. Notably, S. aureus was more susceptible than A. triangularis and P. aeruginosa to P. betle extract treatment. Unexpectedly, our study revealed that S. aureus negatively affected A. triangularis in the co-culture after 3 days of incubation, whereas P. aeruginosa facilitated the growth of A. triangularis in the presence of the extract. This study provides compelling evidence of the anti-adhesive and anti-Acanthamoeba properties of P. betle leaf extract against A. triangularis under monoculture and co-culture conditions. The observed impact on Acanthamoeba cell membranes, coupled with the time-kill kinetic assay results, underscores the potential of P. betle leaf extract as a promising agent for combating Acanthamoeba-related infections in humans and animals.

The potential of a self-nano emulsifying drug delivery system for ethanol extract of red betel vine as an antibacterial agent against Mycobacterium tuberculosis and Pseudomonas aeruginosa

The potential of a self-nano emulsifying drug delivery system for ethanol extract of red betel vine as an antibacterial agent against Mycobacterium tuberculosis and Pseudomonas aeruginosa

Total Phenolic content and Potential of Black Betel Leaf (Piper betle L.var Nigra) Extract as an Antioxidant and Anti-Gout

Research to find antioxidants and anti-gout has been done using black betel leaf. The sample was extracted at room temperature for 30 minutes using the Ultrasound-Assisted Extraction (UAE) method, and a yield of 8.47% was obtained. Phytochemical screening was carried out on the ethanol extract of black betel leaves and showed the presence of secondary metabolite compounds consisting of alkaloids, phenolics, saponins, tannins, and steroid glycosides, with a total phenolic content of 758,534±0.003mg GAE per gram of sample. Antioxidant activity analysis was carried out using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods, which showed IC50 and EC50 values of 115.562±0.14mg/L and 119.273±0.385 mg/L, respectively. The antioxidant activity of black betel leaf extract is in the medium category (100–150 mg/L). The anti-gout activity test was carried out using allopurinol as a control, which showed the ability of black betel leaf extract to reduce uric acid up to 14.48±0.01%. The ability of black betel leaf extract as an anti-uric acid is five times better than allopurinol, which is commonly used as a gout drug. The research results obtained opened up the opportunity to find a natural medicine for gout.

Healing Effect of Wistar Rat Incision Wounds on Administration of Microemulsion Combination of Green and Red Betel Leaf Extracts

One natural ingredient that has the potential to heal wounds is betel leaf. This study aimed to analyze the healing effect of Incision wounds in Wistar rats when administering a microemulsion combination of green and red betel leaf extracts. This research begins with sample collection, drying process, and extraction. The extract was then formulated into emulgel as a microemulsion in three formulations. These three formulas, along with a positive control and a negative control, were tested on each Wistar rat incision wound. The results of the research showed that emulgel formula 3, which was tested on mice, had the best ability to heal wounds for an average of 2.5 days and kept the mice's body temperature normal; 36.6oC. Formula 3 contains 5% South Kalimantan red betel, 5% South Kalimantan green betel, 5% Central Kalimantan red betel, and 5% Central Kalimantan green betel. This proves that the combination of formula 3 can heal wounds and body temperature in experimental mice. Further research is needed to find the best composition that produces faster, more complete wound healing effects and safely impacts experimental animals.

The capability of Piper betle leaves ethanolic extract to inhibit neutrophil infiltration in sepsis-induced BALB/c Mice

Background: Sepsis due to ESBL-producing Escherichia coli infection requires innovative management to prevent a higher prevalence of meropenem overuse. The antimicrobial effect of Piper betle leaves ethanolic extract has been previously proven and should be tested on sepsis-induced animal models. Objectives: This study assesses the antimicrobial effect of Piper betle leaves ethanolic extract towards the bacterial loads and its anti-inflammatory effect on neutrophil infiltration in the lung, kidney, and liver of sepsis-induced mice. Methods: After sepsis inducement, 3 mg/mL of Piper betle leaves ethanolic extract was administered orally. After the mice died, the bacterial load of the lung, kidney, and liver was measured, while neutrophil infiltration was observed using hematoxylin-eosin staining. Results: There is no significant decreasing the number of bacterial loads in the treatment group but there is a significant decrease in the number of neutrophils infiltration in the treatment group. Conclusion: Single dose administration of Piper betle leaves ethanolic extract is sufficient to inhibit neutrophil infiltration significantly.

Effectiveness of Administering Red Belt Leaf Extract (Piper Crocatum) Against Aeromonas Hydrophila Bacteria in Vitro

Diseases caused by pathogenic bacteria such as Aeromonas hydrophila are a major health concern. However, the use of red betel leaves (P. crocatum) can serve as a potential solution to this problem due to their antibacterial properties. This research aimed to study the antibacterial activity of red betel leaf extract (P. crocatum), the structure and character of the bacteria inhibited by the extract, and the bioactive components present in the leaves. The results of the disc test showed that the average zone of inhibition was 14.28 mm, while the MIC test results revealed that a dose of 100 mg/L could inhibit bacterial growth (bacteriostatic). Moreover, the crude extract of red betel leaf (P. crocatum) damaged the cell walls and cell membranes of A. hydrophila bacteria, thereby affecting their character and structure. The UV-VIS analysis of red betel leaf extract (P. crocatum) and FTIR analysis with 96% ethanol absorption indicated the presence of flavonoids, saponins, tannins, and alkaloids. These findings suggest that red betel leaves can be used as a natural remedy to fight bacterial infections caused by pathogenic bacteria such as Aeromonas hydrophila

Pemberdayaan Kelompok Pembudidaya Ikan Desa Pakowa Bunta dengan Menggunakan Ekstrak Daun Sirih (Piper betle L.) dalam Pakan pada Budidaya Ikan Nila Sistem Bioflok

Pakowa Bunta Village is one of the villages in Nuhon District, Banggai Regency, physically it is a lowland area located on the coastline. The large area of tilapia ponds in Pakowa Bunta Village and the suboptimal management of tilapia cultivation have caused many tilapia fish to die. The repeated failure of cultivation in the traditional system of tilapia cultivation in Pakowa Bunta Village requires the transfer of innovative fish cultivation technology from the traditional system to the biofloc system. This community service activity aims to improve the skills and knowledge of fish cultivator groups regarding biofloc system fish cultivation and increase the economic income of fish cultivators in Pakowa Bunta Village. The approach used to resolve partner problems in this activity is the Community Development method, which is oriented towards developing community empowerment by making the community the subject and object of development and direct involvement in various service activities. The final result achieved from this community service activity is an increase in the skills of the fish cultivator group in innovating tilapia cultivation technology using a biofloc system that is modified by using betel leaf extract in the feed given to tilapia, resulting in an increase in tilapia production and an increase in the community's economic income of fish farmers.

Determinants of Productivity and Problems Associated With Betel Leaf Cultivation in Sylhet Region of Bangladesh

The study aimed at evaluating the determinants of productivity of betel leaf cultivation among the farmers of two districts namely Sylhet and Moulvibazar administered under Sylhet division, Bangladesh. A total of 80 respondents were selected as the sample using multistage random sampling technique for achieving objectives of the study. Data were collected through a structured questionnaire during the month of March, 2020. Based on the objective’s descriptive statistics, ANCOVA and stepwise regression model were employed to analyze the data. The study found that, most of the farmers of betel leaf were followed their indigenous knowledge and technology to cultivate betel leaf. ANCOVA and stepwise regression model were employed to identify the factors as well as their impact on betel leaf production. The study identified the factors that significantly influenced the productivity of betel leaf farming i.e. the involvement of marketing agreement, access to training, access to irrigation, amount of cow dung applied and land area under betel leaf cultivation. The study also confronted some problems associated with the betel leaf cultivation like excess rainfall during peak season, insufficiency of irrigation water, disease attacks and low price of betel leaf. Therefore, this study provide essential information that can be used by the growers, scientists, exporters, policy makers to increase the productivity of betel leaf cultivation in the future. This study will build a foundation for further research based on the present background.

Pemanfaatan Ekstrak Daun Sirih Hijau (Piper Betle Linn.) sebagai Capping Agent dalam Green Synthesis Spinel Ferit ZnFe2O4 untuk Remediasi Fenol dalam Air dan sebagai Anti Bakteri

The spinel ferrite material ZnFe2O4 was synthesized using a green synthesis approach by the hydrothermal method. In the synthesis, 2M NaOH was used as a mineralizer and betel leaf extract with varying volumes of 1, 3, 5, and 7 mL as a capping agent, then continued with calcination at 500, 600, and 700°C. Several devices, such as XRD, FT-IR, SEM-EDX, DRS-UV-Vis, and VSM, were used to characterize the synthesized ZnFe2O4 material. The pattern of XRD indicated that the ZnFe2O4 material has a cubic structure, where the increase in crystal size was after calcination. The Fourier Transform Infra-Red (FT-IR) spectrum shows the Fe-O interaction at wave numbers 427-417 cm-1, which is located on the octahedral side, while the Zn-O interaction at wave numbers 534-510 cm-1, which is located on the tetrahedral side in spinel ferrite structure. From the DRS UV-Vis spectrum pattern and band gap values, it was found that the material absorbs light in the visible region. Scanning Electron Microscope (SEM) images show that the morphology of the synthesized material is circular. The Vibrating Sample Magnetometer (VSM) hysteresis curve shows that the synthesized ZnFe2O4 has paramagnetic properties. The synthesized ZnFe2O4 material has photocatalytic activity towards phenolic compounds with a degradation percentage reaching 62.2%, and this material is active as an antibacterial with an inhibition area of 14.4 for S. aereus bacteria.

Comparison of Organic Liquid Soap Made from Papaya-Turmeric and Aloe Vera-Piper Betel

<p>In the wake of the COVID-19 pandemic, the imperative to mitigate pathogen transmission has catalyzed the innovation of soaps imbued with antimicrobial and antibacterial properties. Diverging from conventional reliance on synthetic chemicals, often associated with adverse dermatological reactions, this study explores the formulation of organic liquid soaps. These soaps leverage naturally occurring antibacterial and antimicrobial compounds from readily accessible plants, presenting a viable alternative to commercially available inorganic body washes predominantly manufactured by small to medium-sized enterprises. Specifically, this research harnesses papaya and aloe vera extracts for their antimicrobial attributes, augmented with turmeric and piper betel for enhanced antibacterial efficacy. Despite the recognized potency of turmeric and piper betel, variability in their effectiveness necessitates rigorous validation against the Indonesian National Standards (SNI) to ensure product quality and safety. This includes comprehensive evaluations of pH levels, density, Total Plate Count (TPC), and Mold and Yeast Count (MYC) to certify the soap's inhibitory capacity against bacterial and fungal proliferation. Employing maceration and hot processing techniques, the formulated papaya-based soap adhered to SNI 4085:2017 criteria for pH and MYC, although initially failing the TPC test. Subsequent incorporation of turmeric extract facilitated compliance with all SNI benchmarks. Conversely, aloe vera formulations only satisfied pH requirements under the SNI, even after adding piper betel. However, substituting piper betel with turmeric extract enabled the aloe vera soap to fulfill the requisite SNI parameters. These findings underscore turmeric extract's superior antibacterial properties, positioning it as a pivotal component in papaya and aloe vera soap formulations. Contrary to prevalent assumptions regarding piper betel's antimicrobial and antibacterial effectiveness, our investigation substantiates turmeric's superior role in bacterial growth inhibition within organic soap matrices.</p>

The Effect of Imbibition on Immersion Hydrocolloid Irreversible Alginate Sodium Hypochlorite Disinfectant Solution and Red Betel Leaf (Pipper Crocatum) Extract

Background: impressing the patient's oral cavity in dentistry can pose a risk of cross infection between doctor and patient. Therefore it is necessary to disinfect the impression material to remove bacteria that stick to the impression material. Disinfection that can be used besides sodium hypochlorite can also extract red betel leaves. Purpose: to find out the difference of imbibition on immersion hydrocolloid irreversible alginate in sodium hypochlorite 1% and red betel leaf extract 100%. Method: this research is experimental with pretest and posttest control group design with 4 treatment groups, 2 groups were immersed in sodium hypochlorite 1% and 2 groups were immersed in red betel leaf extract 100%. Immersing time is 5 and 10 minutes. Each repetition is done 6 times. Negative molds are measured before and after immersion. Data were analyzed using the OneWay Anova Test. Results: The effects of alginate mold imbibition on sodium hypochlorite immersion were greater than immersion in red betel leaf extract. Conclusion: There was a significant difference (p <0.05) in the imbibition of the printed hydrocolloid irreversible alginate soaked in sodium hypochlorite 1% and red betel leaf extract 100% for 5 minutes, and 10 minutes

The Phytochemistry profile of Piper betle extract and its Activity Against Hepatitis C Virus

Hepatitis C virus (HCV) is an RNA virus that is easy to mutate and high risk of resistance. The vaccine for HCV has not been provided yet, and the current treatment is very expensive. To provide alternative and complementary antiviral from plants, this study evaluated the activities of Piper betle (P. betle) against HCV and its combination with existing antiviral drugs, Ribavirin and Simeprevir. The antiviral inhibition was identified by in vitro culture using Huh7it-1 cells and JFH1a HCV. Moreover, the phytochemistry profile was also determined by Thin Layer Chromatography (TLC) and High-Performance Liquid Chromatography (HPLC). The result showed that the ethanol extract of P. betle was possess strong activity with an IC50 value of 0.08 ± 0.028 µg/mL. The mechanism of action revealed the extract dominantly inhibit in the post-entry steps. Furthermore, the combination of P. betle extract with simeprevir increased its anti-HCV activity, however, no effect was observed in the combination with ribavirin. The Western blotting analysis was shown inhibition of NS3 protein levels in a dose-dependent manner. Phytochemistry evaluation was shown for the extract containing flavonoids, polyphenols, and alkaloids. These results suggested that the ethanolic extract of P. betle could be a good candidate for the development or an alternative to anti-HCV drugs.
 Keywords: Hepatitis C Virus, Piper betle, Medicinal plants, Simeprevir, Ribavirin, Medicine.

Fungal diseases of Betel vine (Piper betle L.) in India and their management : An overview

Fungal diseases of Betel vine (Piper betle L.) in India and their management : An overview

Evaluation of the Effects of Piper betle Supplementation as a Natural Antibiotic Growth Promoter (NAGP) on Antimicrobial Activity, Feed Acceptance and Growth Performance of Kelah (Tor sp.) in a Tank System

Evaluation of the Effects of Piper betle Supplementation as a Natural Antibiotic Growth Promoter (NAGP) on Antimicrobial Activity, Feed Acceptance and Growth Performance of Kelah (Tor sp.) in a Tank System