Betel Research Articles

Innovation in Idli batter preservation through betel leaf essential oil-based nanoemulsion: Evaluating fermentation kinetics and physicochemical analysis

Extending shelf life while maintaining quality of traditional fermented foods is a major challenge. This study aimed to investigate betel leaf essential oil nanoemulsions (BLEONE) as a clean-label preservative for fermented Idli batter. BLEONE (1–10 %) was incorporated into Idli batter and evaluated for physicochemical properties, sensory quality, and shelf life during ambient (30 °C) and refrigerated (4 °C) storage. Kinetic modeling and analysis of variance (ANOVA) were used to quantify BLEONE's effects on fermentation dynamics and assess statistical significance. At 5 % concentration, BLEONE significantly (p < 0.05) maintained pH (4.5 vs 3.8 in control), restricted acidity increases (1.2 % vs 1.8 % in control), and limited viscosity loss (15 % vs 40 % in control) during fermentation compared to untreated and sodium benzoate-treated samples. Sensory analysis showed acceptable attributes up to 5 % BLEONE. Optimally fermented Idli batter with 5 % BLEONE extended shelf-life to 7 days at 30 °C and 25 days at 4 °C storage. The nanoemulsion's enhanced antimicrobial efficacy and controlled release of active compounds significantly improved safety and quality preservation during storage. This research demonstrates the potential of essential oil nanoemulsions as effective clean-label preservatives for traditional fermented foods, offering a promising solution to extend shelf life while maintaining product quality.

Development and characterization of sodium alginate and β-cyclodextrin nanoemulsions encapsulating betel leaf (Piper betle L.) extract for enhanced antimicrobial efficacy against foodborne pathogen

This study aims to investigate the physical stability, droplet size, zeta potential, and antimicrobial properties of nanoemulsions formulated with betel leaf extract using β-cyclodextrin (CD) and sodium alginate (SA) biopolymers. Nanoemulsions with β-cyclodextrin exhibit superior stability at lower temperatures, with limited droplet size, and strong electrostatic repulsion. Morphological images demonstrate the successful encapsulation of betel leaf extract within both biopolymers, highlighting their potential for antimicrobial applications. Both CD and SA nanoemulsions display inhibitory effects on bacterial strains (E. coli, P. aeruginosa, L. monocytogenes, S. aureus, and B. cereus) and fungal growth (A. brasiliensis, R. stolonifer, F. oxysporum, and C. albicans). SA nanoemulsions show higher antimicrobial activity due to H+ ion release, particularly against A. brasiliensis and C. albicans. These findings underscore the potential of betel leaf extract nanoemulsions, especially those with SA, for various antimicrobial applications for sustainable food packaging, highlighting their significance in addressing microbial challenges.

Evaluation of DSM-5 Diagnostic Criteria for Betel-Quid Use Disorder Using the Addiction Characteristics Defined by American Society of Addiction Medicine

Background The Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5), categorizes betel-quid use disorder (BUD) under Other (Or Unknown) Substance Use Disorder, and the diagnostic criteria used are adapted from those of Substance Use Disorder. Because different substances have different characteristics, an improved set of diagnostic criteria is required to better detect BUD. Objective The objective of this study was to examine the different measures of accuracy for DSM-5 BUD by using the addiction characteristics defined by the American Society of Addiction Medicine (ASAM). Methods A certified psychiatrist conducted face-to-face diagnostic interviews. Questionnaires were administered to assess betel-quid use history, patterns of use, and dependence features. All betel-quid users were evaluated for BUD by using the DSM-5 criteria and addiction characteristics defined by the ASAM. Results One of the DSM-5 diagnostic criteria for BUD, namely large amount of time spent on obtaining and using betel quid and recovering from betel-quid use, showed the lowest sensitivity of 0.14, lowest diagnostic accuracy of 0.63, and lowest diagnostic odds ratio of 2.61. Another DSM-5 diagnostic criterion, namely continued betel-quid use despite knowledge of physical or psychological problems, had the lowest specificity of 0.49. The diagnostic threshold of five or more DSM-5 BUD criteria showed a sensitivity of 0.86 and a specificity of 0.97. Conclusions This study is the first to evaluate the different measures of accuracy for DSM-5 BUD. Given that each addictive substance has unique addictive characteristics, the composition and number of criteria for diagnosing DSM-5 BUD must be reconsidered.

A Review on Production and Marketing of Betel Vine

Betel Vine is grown widely across the country, playing an essential role in social, religious, and cultural activities and significantly contributing to the economy through employment and foreign exchange. This review article on Betel Vine, a significant economic and heritage crop in India, employs a systematic literature review to analyze and synthesize existing research on its production and marketing. Betel Vine, with its heart-shaped leaves known as "Paan," provides substantial employment and foreign exchange. The review identifies that the marketing of Betel leaves faces unique challenges due to their perishable nature and specific regional preferences. The systematic literature review provides a comprehensive understanding of the trends, challenges, and opportunities in Betel Vine production and marketing. Key findings indicate significant economic contributions from states like Karnataka, Tamil Nadu, and West Bengal. The review also identifies research gaps, such as the need for improved marketing strategies and better management of production challenges. Future research should focus on developing value-added products, enhancing marketing efficiency, and addressing health concerns associated with Betel chewing. The conceptual framework guides the review process, synthesizing diverse literature to facilitate a structured and comprehensive analysis. The outcomes suggest that effective strategies for enhancing the Betel Vine industry should address these identified gaps and leverage opportunities to boost economic growth and support rural communities.

The color features and k-nearest neighbor algorithm for classifying betel leaf image

Piper betle L. (betel) is a species that belongs to the genus Piper and is a type of medicinal plant that is quite well known to the general population. The varieties of the leaf color may distinguish are red, green, and black betel. However, consumers still need assistance determining the differences between the many types of betel leaf. Therefore, using image processing techniques, this research contributes to building a classification method for distinguishing betel leaves based on color attributes. This approach anoints for the region of interest detection, feature extraction, and classification. In addition, three different classifiers, naïve Bayes, support vector machine, and k-nearest neighbors (k-NN), were used during the classification process. The evaluation for this study used a percentage split to divide a total of 180 images between the training and testing phases. The method’s performance provided the highest accuracy value possible, 100%, by utilizing the color characteristics with the k-NN classifier.

790. RISK FACTORS FOR ESOPHAGEAL CANCER IN NORTHEAST INDIA: A MULTI-CENTRIC CASE-CONTROL STUDY

Abstract Background Northeast India has a high incidence of esophageal cancer(EC), with age-adjusted incidences ranging from 19 to 50 per 100,000 (in females and males respectively). This is over ten times the average incidence of EC in India. The local preparation of raw betel nut,‘kwai’, is integral to the culture, but its role has not been established. This study aimed to assess the role of betel nut, with or without tobacco as a causative agent for EC. The role of alcohol was also studied. Methods A multi-centric, matched case-control study was performed from October 2022 to March 2024 in three centres situated in the Northeast region of India. All cases of biopsy proven squamous EC, arising from the cricopharynx to the gastroesophageal junction were included, with matched controls (1:3 ratio) being patients visiting the hospital for minor ailments and healthy volunteers from the community. A validated questionnaire was administered by trained trial coordinators. The data was analysed using a conditional multivariate logistic regression which incorporated the individual matching of cases with 3 controls. A p-value of &amp;lt; 0.05 was considered statistically significant. Results A total of 185 cases and 556 controls were matched for age (± 5 years), ethnicity, and gender. There was an increased risk of EC for those consuming betelnut with slaked lime [Calcium hydroxide Ca(OH)2] (Odds Ratio(OR) of 3.77 (Confidence Interval(CI) 1.30-10.92)); alcohol (OR 2.00 CI: 1.80-6.67) and tobacco (OR 3.47 CI:1.80-6.67). The risk was higher for those who swallowed the betel quid and juice as compared with those who spit it out (OR 4.01 CI: 2.24-6.73). The risk was increased in those consuming betelnut while hungry, instead of a meal (OR 10.40 CI 3.66-29.57). The risk was increased in those with no formal education (OR of 4.13 CI: 2.01-8.49). On adjusting with other risk factors (tobacco and alcohol), the risk was highest for betelnut with tobacco (OR 7.84 CI 2.12-29.90) and betelnut with alcohol (OR 2.84 CI 1.11-7.29). All these OR were statistically significant. Conclusion Consumption of betelnut with slaked lime increases the risk of EC. This risk is increased by the concomitant use of tobacco and alcohol.

Valorization of betel leaves byproducts: A novel ingredient for sustainable cookie development

Environmental sustainability in food production requires innovative methods to repurpose byproducts. This study examines betel leaf residue as a sustainable ingredient to improve the nutritional value and sensory attributes of cookies. A randomized controlled trial was conducted where betel leaves powder after extraction (BLPAE) was incorporated into cookie formulations at varying proportions (3% to 10%). The experimental design utilized a completely randomized design (CRD) to ensure unbiased distribution of treatments. The effects of BLPAE incorporation were interpreted using analysis of variance (ANOVA) to identify significant differences between means, followed by duncan's multiple range test (p ≤ 0.05) to compare the treatment groups. Additionally, sensory evaluations were performed using a 9-point hedonic scale with trained panellist to assess the acceptability of the fortified cookies. Cookies with 5% BLPAE showed a 571.42 ± 8.57% increase in fiber content. Pasting properties reduced peak viscosity (103.10 cP to 69.58 cP) and increased dough hardness (3481.6 g to 8577.97 g), enhancing texture with overall acceptability of 7.5, without compromising taste and texture. In conclusion, incorporating 5% BLPAE into cookie formulations optimally enhances nutritional value while maintaining desirable sensory attributes, highlighting betel leaf residue as a sustainable ingredient for innovation in bakery products.

Quality evaluation of Idli prepared from betel leaves essential oil nanoemulsion treated stored batter

The demand for clean-label, natural preservation in fermented foods requires innovative methods to extend shelf-life while maintaining quality. This study evaluates Idli quality from batter stored with and without 5% betel leaf essential oil-based nanoemulsion (BLEONE) at 30 °C and 4 °C. Color, texture, microbial growth, proximate composition and sensory attributes were assessed using standard methods, with statistical analysis via ANOVA and Tukey's post-hoc test (p < 0.05). BLEONE-treated Idli showed 30.2% reduced hardness, 17.1% better color retention, and 0.61 log CFU/g less microbial growth after 5 days, inhibiting harmful microbes and supporting beneficial lactic acid bacteria. At 30 °C, BLEONE-treated Idli maintained quality for 7 days versus 2 days for controls. Moisture, protein, and fat retention improved by 3.4%, 7.3%, and 6.2%. Sensory scores for taste, mouthfeel, and acceptability increased by 3.5%, 4.1%, and 3.8% after 10 days at 4 °C. BLEONE-treated batter produced acceptable Idli for up to 25 days at 4 °C, compared to 10 days for controls, extending shelf-life by 150%. This study shows BLEONE is an effective natural preservative for Idli, extending shelf-life and maintaining quality at ambient and refrigerated temperatures. These findings suggest potential for clean-label preservation methods for fermented foods, potentially revolutionizing the food preservation industry.

Total Phenolics, Flavonoids, and ɑ-Glucosidase Inhibitory Activity of Red Betel (Piper crocatum) Extract in Various Solvents

Alpha-glucosidase enzyme activity needs to be inhibited to avoid increasing blood sugar levels in diabetics. Red betel leaf extract is reported to have active compounds such as phenolics and flavonoids that have alpha-glucosidase enzyme inhibition activity. Previous research showed that 70% betel leaf extract has an ɑ-glucosidase enzyme inhibition activity of 69.84%, but it is not yet known how the effect of extracts with different levels of polarity on the inhibition activity of alpha-glucosidase enzyme. This study aimed to determine extracts that have optimal levels of phenolic and flavonoid content and the highest suppression of α-glucosidase activity. The multistage maceration method was used to extract red betel leaf simplicia using solvents with different levels of polarity (n-hexane, ethyl acetate, methanol). Tests were conducted on overall phenolic concentration, flavonoids, and ɑ-glucosidase inhibition ability. Data were analyzed using one-way ANOVA test with Tukey's further test (p&lt;0.05). The results of the assessment of phenolic concentration, flavonoids, and α-glucosidase inhibitory activity as a whole showed statistically significant differences (p&lt;0.05) in all extracts with the highest total phenolic and flavonoid content found in the n-hexane extract of 25.68 ± 0.29 mg GAE/g and 65.84 ± 0.96 mg QE/g respectively. Meanwhile, methanol extract of red betel leaf became the best extract in this study with the greatest inhibition among all extracts with a percent α-glucosidase inhibition value of 43.87 ± 1.83%.

Efektifitas Rebusan Air Daun Sirih Dalam Perawatan Dermatitis Dalam Konsep Asuhan Keperawatan di Kelurahan Pandan Kasturi RT/RW: 01/01 Kecamatan Sirimau Kota Ambon

Occupational skin disease is a skin disorder that occurs due to a person's work and is commonly found in industrial, agricultural, and plantation areas. This disease is caused by materials/substances and processes carried out in the workplace generally and is known as dermatitis. Atopic dermatitis has dry skin and often has itching, allergic rhinitis, nasal obstruction, conjunctiva and pharynx itching, lacrimation, and seasonality when associated with pollen. This study uses a descriptive case study method where the researcher will describe the case experienced by a client with dermatitis. Respondents in this case study were Mr. N who has been experiencing dermatitis for ± 2 weeks in the pandan kasturi village RT / RW: 01/01 which was carried out for 3 days (November 27, 2023-30 November 2023). The results showed that during the 3 days of implementing dermatitis treatment with siri leaf decoction water, there were changes from Mr. N's complaints. Mr. N said the itching on his feet and hands and between his fingers decreased, did not feel itchy at night after treatment with siri leaf decoction water, redness and wounds due to frequent scratching seemed to decrease. It can be concluded that betel leaf cooking water is effective for the treatment of itching in dermatitis.

Impact of Matrix Metalloproteinase-8 Polymorphisms on Nasopharyngeal Carcinoma Risk.

Upregulation of matrix metallo-proteinase-8 (MMP-8) serves as a protein-based indicator for predicting nasopharyngeal carcinoma (NPC) metastasis. Nevertheless, the role of MMP-8 genotypes in NPC has never been investigated. This study aimed to explore the involvement of MMP-8 genotypes in NPC development. We employed the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to analyze MMP-8 genotypes, specifically C-799T (rs11225395), Val436Ala (rs34009635), and Lys460Thr (rs35866072), in a Taiwanese cohort comprising 208 NPC cases and 416 healthy controls. Individuals with either heterozygous or homozygous variant genotypes of MMP-8 rs11225395 showed no significant change in NPC risk compared to those with the wild-type genotype [odds ratio (OR)=0.97 and 0.79, 95% confidence intervals (95%CI)=0.68-1.38 and 0.46-1.36; p=0.9304 and 0.4736, respectively]. Similarly, there was no significant association between the heterozygous genotypes of MMP-8 rs34009635 and NPC risk (OR=0.66, 95%CI=0.24-1.84; p=0.5738). For MMP-8 rs35866072, all individuals in the study were of the TT genotype. Furthermore, the presence of variant alleles at MMP-8 rs11225395 or rs34009635 did not result in altered NPC risk (OR=0.91 and 0.66, 95%CI=0.71-1.16 and 0.24-1.84, p=0.4876 and 0.5769, respectively). Additionally, no significant association was observed between MMP-8 rs11225395 variant genotypes and NPC risk among individuals regardless of smoking, alcohol consumption, or betel quid chewing habits (all p>0.05). There was no association between the MMP-8 genotypes rs11225395, rs34009635, or rs35866072 and NPC risk among Taiwanese individuals. Moreover, no combined effects of MMP-8 genotype with smoking, alcohol consumption, or betel quid chewing habits on NPC risk were observed.

Chemical, physical, and antibacterial properties of alginate-based edible film with red betel leaf extract

Edible films are biodegradable and environmentally friendly, making them ideal for food packaging; however, they often exhibit low water resistance and poor water vapor barrier properties. This study aims to enhance alginate-based edible films by incorporating ethanol extract from red betel leaves known for their antibacterial properties. The effects of red betel leaf extract on the chemical, physical, and mechanical properties of these films were evaluated, in addition to their antibacterial activity. Concentrations of the extract tested were 0%, 10%, 12.5%, and 15%. The films were assessed for various properties, including moisture content, water vapor transmission rate (WVTR), water solubility, colour, opacity, thickness, tensile strength, and elongation at break. The antibacterial efficacy was tested against Escherichia coli, Staphylococcus aureus, Salmonella enterica, and Pseudomonas aeruginosa. Scanning electron microscopy (SEM) was used to analyze the impact of the extract on film surface morphology. Adding red betel leaf extract significantly impacted the thickness, WVTR, moisture content, water solubility, colour, and opacity of the films. Notably, incorporating the red betel leaf extract improved the WVTR and water solubility, with the 12.5% concentration showing the greatest antibacterial effectiveness.

Impact of MAOA Gene Polymorphism on the Efficacy of Antidepressant Treatment and Craving Severity for Betel Quid Use Disorder.

Betel quid (BQ) use disorder (BUD) is prevalent in many Asian countries, impacting approximately 600 million people. We conducted a randomized clinical trial to analyze the impact of MAOA genetic variations on the severity of BQ craving. This was measured using DSM-5 criteria and the Yale-Brown Obsessive-Compulsive Scale modified for betel quid use (Y-BOCS-BQ). Participants were grouped according to the severity of BUD and MAOA gene single-nucleotide polymorphism (SNP) rs5953210 genotypes. The Y-BOCS-BQ scores were assessed at baseline (week 0) and during follow-up at weeks 2, 4, 6, and 8. The AA genotype group showed significantly greater reductions in Y-BOCS-BQ at weeks 2 (p = 0.0194), 4 (p = 0.0078), 6 (p = 0.0277), and 8 (p = 0.0376) compared to the GG genotype group. Additionally, within the antidepressant group, the AA genotype showed significant reductions in the Y-BOCS-BQ scores at weeks 2 (p = 0.0313), 4 (p = 0.0134), 6 (p = 0.0061), and 8 (p = 0.0241) compared to the GG genotype. The statistical analysis revealed a significant interaction between the treatment and placebo groups based on MAOA genotypes, with the AA genotype in the treatment group exhibiting a more pronounced decrease in Y-BOCS-BQ score (p interaction <0.05) at week 6. Our study highlights the importance of considering genetic factors when developing personalized treatment plans for BUD.

Betel-quid addictive use disorders and Oral potentially malignant disorders and Oral cancer in south, southeast, and East Asia: A systematic review and meta-analysis.

This study aimed to provide a comprehensive assessment of the measurement and prevalence of betel-quid (BQ) abuse, dependence, and BQ use disorder (BUD), as well as to evaluate the impact of BQ addiction on oral malignant diseases. We used the PRISMA guidelines to perform a systematic review and meta-analysis. We searched for relevant publications up to April 2024 in PubMed, Web of Science, and Embase. The articles were evaluated for BQ addiction and its relationship with oral potentially malignant disorders (OPMD) and oral cancer. The prevalence of BQ abuse, dependence, and BUD in South, Southeast, and East Asia varied between 0.8%-46.3%, 0.4%-43.5%, and 4.7%-39.2%, respectively. Among BQ chewers, the corresponding proportions of these disorders ranged from 40.5%-99.6%, 20.9%-99.6%, and 55.2%-99.3%. The pooled risks of OPMD associated with BQ abuse, dependence, and BUD were 16.3, 18.7, and 9.6-35.5, respectively. The risk of oral cancer for mild, moderate, and severe BUD was 8.5, 8.2, and 42.3, respectively. BUD mediates the link between BQ use and an increased risk of oral malignant disorders. Addressing and treating BQ addiction is an important component of comprehensive OPMD and oral cancer preventive and intervention programs that go beyond simple cessation efforts.

SHIELDING BANGLADESH'S EXPORTABLE BETEL LEAVES: MANAGING E. coli AND Salmonella CONTAMINATION

The study examined the presence of E. coli and Salmonella spp. in soil, water, and mature betel leaf samples at preharvest stages across three upazilas in Kushtia district: Kushtia Sadar, Mirpur, and Daulatpur, although these pathogens do not typically affect betel leaf and other fresh vegetables. Researchers tested the samples before and after sanitization with calcinated calcium (CCa) from May to September 2020. They collected soil, water, and betel leaf samples from 15 farmers' fields, and initially, all samples were contaminated with E. coli and Salmonella spp. After sanitization, E. coli contamination persisted in a few samples: 22.22% in Sadar, 28.89% in Mirpur, and 35.56% in Daulatpur, while Salmonella spp. was eradicated. Potential contamination vectors included dogs (86.67%), birds (77.78%), and cats (59.26%). In Dhaka, betel leaves from the central pack house showed significant contamination differences post-washing. Before washing, 51.67% of leaves had E. coli and 41.50% had Salmonella spp. After three washes with CCa, contamination was completely eliminated for both pathogens. The study highlights the effectiveness of CCa in reducing microbial contamination but also points out the need for better contamination control strategies and training for farmers to mitigate vector-related contamination. This comprehensive study highlights the effectiveness of CCa in reducing microbial contamination in betel leaf production and underscores the need for ongoing management to mitigate recontamination risks from environmental vectors.

Medicinal Plants and Natural Products to Treat Obesity through Inhibiting Pancreatic Lipase: A Review (2020-2022)

: Pancreatic lipase is an enzyme that catalyzes the hydrolysis of triglycerides to monoglycerides and free fatty acids which promote and accelerate their absorption by the intestine, thus leading to obesity. Drugs that have numerous side effects explain the beneficial medicinal effect of plants resulting from their Phyto molecules that exhibit strong anti-lipase activity. The present review reveals the medical treatment and consequently the associated side effects. It also represents an update of various medicinal plants and their metabolites that act as lipase inhibitors published between (2020-2022). We have discussed 93 species belonging to 48 different plant families and numerous bioactive molecules exerting this activity. We have compared 29 species for their anti-lipase potential. Fabaceae and Lamiaceae were the most dominant with 7 species, and the highest percentage (95%) for pancreatic lipase inhibitory activity was recorded by “Filipendula kmtaschatia” from Rosaceae family while “Piper betle” from Piperaceae family showed the lowest percentage (15.9%). The medical treatments with low dose effect were liraglutide saxenda (3mg/day), also flavonoids, in particular catechin derivatives, which were the most potent in terms of pancreatic lipase inhibitory activity with the lowest IC50s. This study summarized medical and natural treatments that are used to treat obesity through inhibiting pancreatic lipase and delaying fat assimilation in the intestines. So far, more studies are needed for the use of these as herbal medicine for obesity. Conclusion: This study summarized medical and natural treatments that are used to treat obesity through inhibiting pancreatic lipase and delaying fat assimilation in the intestines. So far, more studies are needed for the use of these as herbal medicine for obesity.

The anti-quorum sensing and biofilm inhibitory potential of Piper betle L. leaf extract and prediction of the roles of the potent phytocompounds

The leaves of Piper betle L., known as betel leaf, have immense medicinal properties. It possesses potent antimicrobial efficacies and can be a valuable tool to combat drug-resistant microorganisms. Quorum sensing (QS) inhibition is one of the best strategies to combat drug resistance. The present study investigates the anti-quorum sensing and biofilm inhibitory potential of Piper betle L. leaf extract against two bacterial strains, Chromobacterium violaceum and Pseudomonas aeruginosa. The extract produced substantial QS-inhibition zones in a biosensor strain of C. violaceum (CV026), indicating interference with quorum-sensing signals. The Results demonstrated significant inhibition in biofilm formation and different QS-regulated virulence factors (violacein, exopolysaccharides, pyocyanin, pyoverdine, elastase) in both C. violaceum and P. aeruginosa at sub-MIC concentrations of the extract and tetracycline, an antibiotic with known anti-QS activity. The quantitative real-time PCR (qRT-PCR) revealed decreased gene expression in different QS-related genes in C. violaceum (cviI, cviR, and vioA) and P. aeruginosa (lasI, lasR, lasB, rhlI, rhlR, and rhlA) strains after treatment. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified the significant phytocompounds, mainly derivatives of chavicol and eugenol, in the extract. Of these compounds, chavicol acetate (affinity: −7.00 kcal/mol) and acetoxy chavicol acetate (affinity: −7.87 kcal/mol) showed the highest potential to bind with the CviR and LasR protein, respectively, as evident from the in-silico molecular docking experiment. The findings of this endeavour highlight the promising role of Piper betle L. as a source of natural compounds with anti-quorum sensing properties against pathogenic bacteria, opening avenues for developing novel therapeutic agents to combat bacterial infections.

Photocatalytic, antioxidant, and antibacterial activities of biocompatible MgO flake-like nanostructures created using Piper betle leaf extract

In this study, biocompatible magnesium oxide (MgO) nanostructures were synthesized using an aqueous solution of Piper betle leaf methanolic extract. The color change during the synthesis process, i.e., colorless to dark brown solution, indicates the formation of MgO nano structures which is further confirmed through UV–Visible spectrum that exhibited characteristic absorption band at 329 nm. Results obtained from morphological analysis reveal that MgO nano flakes (MgO NFs) are present as triangular and polyhedral shapes having an average size ranging between 150 and 200 nm with clear lattice fringes. Secondary metabolites were clearly identified from the FTIR spectrum, and they helped in reduction and stabilization during the synthesis of MgO NFs. The XRD pattern displayed Braggs planes of (111), (220), (220), (311), and (222) with a face centered cubic (FCC) structure, which was further confirmed from the SAED pattern using HRTEM analysis. The as-synthesized MgO NFs manifested profound antimicrobial activity against gram positive as well as gram-negative bacteria with favorable biocompatibility. Furthermore, methylene orange dye was degraded almost completely (98.36 %) in the presence of MgO NFs photo catalysts within 1 h of sunlight illumination. In addition, MgO NFs displayed high level of antioxidant properties with recorded IC50 value of 15.89 μg/ml.

SIMPLE ELECTROSPINNING ASSEMBLY FOR THE PREPARATION OF POLYVINYL ALCOHOL NANOFIBERS CONTAINING PIPER BETLE (L)

Objective: The aim of this research was to assemble an electrospinning device with some components from used medical devices in hospital, so that it could be utilized to produce nanofibers containing Piper betle (L). Methods: The electrospinning was assembled with the main components were the 20 kV high voltage (hV) power supply (module), the Terumo TE-331 syringe pump and the collector. The resulting device was then evaluated for tool performance. The device was used to produce Polyvinyl Alcohol (PVA)-based nanofibers with Piper betle (L) as the active ingredient. The nanofibers produced were then tested for antibacterial activity, morphology by Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Results: The polymer solution was pushed by a syringe pump with a flow rate of 15 ml/h into the spinneret. In this electrospinning process, the formed nanofibers appear visually as a thin layer on the collector. With variation in PVA concentration and the same concentration of Piper betle l. as the active ingredient, the results showed that the nanofiber carrier did not affect the efficacy provided by Piper betle (L). The characterization with SEM revealed that the assembled tool was able to make nanofiber preparations that have fine continuous/fairly regular fibers with an average diameter of 46.479±2.406 nm. Meanwhile, the analysis using FTIR showed the presence of OH stretching groups of phenolic compounds from Piper betle (L). Conclusion: It can be concluded that the electrospinning was successfully assembled from unused medical devices in hospital and proven to produce nanofibers.

Encapsulation of anti-bacterial Piper betle leaf extract in thermo-sensitive and biodegradable chitosan hydrogels: synthesis, characterization and release kinetics

Encapsulation of anti-bacterial Piper betle leaf extract in thermo-sensitive and biodegradable chitosan hydrogels: synthesis, characterization and release kinetics