Piper Amalago Research Articles

Influence of seasonal variation on the chemical composition of Piper amalago essential oils and their phytocytogenotoxic activity in model plants and weeds

Influence of seasonal variation on the chemical composition of Piper amalago essential oils and their phytocytogenotoxic activity in model plants and weeds

The Cardiorenal Effects of Piper amalago Are Mediated by the Nitric Oxide/Cyclic Guanosine Monophosphate Pathway and the Voltage-Dependent Potassium Channels.

Piper amalago L. is used in Brazilian traditional medicine to treat inflammation, chest pain, and anxiety. This study aimed to investigate the safety and the renal and cardiovascular effects of the volatile oil (VO) and the aqueous (AE) and hydroalcoholic (HE) extracts from P. amalago. The gas chromatography-mass spectrometry analyses identified 47 compounds in the VO, with β-cyclogermacrene, spathulenol, β-phellandrene, and α-pinene standing out. Among the 47 compounds also found in AE and HE by liquid chromatography-mass spectrometry, glycosylated flavones, organic acids, amino acids, and amides were highlighted. Some examples of these compounds are methoxy-methylenedioxy cis-cinnamoyl pyrrolidine, methoxy-methylenedioxy trans-cinnamoyl pyrrolidine, and cyclobutene-2,4-bis-(1,3-benzodioxol-5-methoxy-6-yl)-1,3-dicarboxapyrrolidide. The acute toxicity experiments were conducted on female rats (n = 5). The cardiorenal assays (n = 8) and evaluations of vasodilatory effects on the mesenteric vascular bed (n = 5) were conducted on male rats. In either extract or VO, there were no mortality or changes in relative weights or histopathological analysis of the organs. Urinary volume and renal electrolyte excretion were elevated significantly during repeated dose 7-day treatment with different preparations from P. amalago. None of the preparations induced hypotension or changes in cardiac electrical activity. Only HE promoted significant vasodilatory effects in rats' isolated mesenteric vascular beds. These effects were completely abolished in the presence of L-NAME plus 4-aminopyridine. Therefore, P. amalago leaves are safe and present diuretic activity after acute and repeated dose administration over 7 days. Moreover, the HE induced significant vasodilator response in rats' mesenteric vascular beds by NO/cGMP pathway and voltage-dependent K+ channels activation.

A Review of Bioactive Compounds and Antioxidant Activity Properties of Piper Species.

Antioxidants are compounds that are able to inhibit the negative effects that come from free radicals. The phenomenon of imbalanced antioxidant production and the accumulation of free radicals in cells and tissues can cause oxidative stress. Excessive free radicals that enter the body cannot be warded off by endogenous antioxidant compounds so that the required antioxidant compounds can come from the outside, which helps in the performance of endogenous antioxidants. Antioxidants that come from outside consist of synthetic and natural antioxidants; however, synthetic antioxidants are not an option because they have toxic and carcinogenic effects. Therefore, the use of natural ingredients is an alternative method that is needed to create a new natural antioxidant compound. Piper species are being considered as possible medicinal plants for the development of new sources of antioxidants. Several studies have been carried out starting from the extract levels, fractions, and compounds of the Piper species, which showed good antioxidant activity. Currently, some of these plants are being used as ingredients in traditional medicines to treat allergies, toothaches, and coughs. This review examines the distribution, botanical data, pharmacology, especially antioxidant activity, and the compounds contained in five Piper species, namely Piper amalago L., Piper betle L., Piper hispidum Sw., Piper longum L., and Piper umbellatum L.

Aximopsis gabrielae sp. nov.: a gregarious parasitoid (Hymenoptera: Eurytomidae) of the skipper Quadrus cerialis (Lepidoptera: Hesperiidae) feeding on Piper amalago in southern Mexico

ABSTRACT Aximopsis gabrielae Zhang, Gates and Campos sp. nov. is described from southern Mexico in the Yucatan Peninsula, Mexico. This species is a koinobiont gregarious larval-pupal endoparasitoid of the caterpillar of Quadrus cerialis (Stoll) (Lepidoptera: Hesperiidae) feeding on the shrub Piper amalago L. (Piperaceae) in the semi-evergreen forest. This is the first record of Aximopsis parasitoids on Hesperiidae (Lepidoptera). Morphological, biological, ecological and geographical data are integrated to delineate the new species. http://www.zoobank.org/urn:lsid:zoobank.org:pub:3226C193-88FB-45EA-BFCB-5C752B3C5E46

Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies

Piper amalago L. (Piperaceae) is traditionally used due to its anti-inflammatory, analgesic, diuretic, and antiparasitic properties. However, few studies have focused on its adverse effects, compromising its safe use. This study evaluated the toxicological safety of ethanolic extract from Piper amalago leaves (EEPA), through subacute toxicity and genotoxicity assays in rodents. In subacute toxicity, 100, 200 or 300 mg/kg of EEPA were tested in female Wistar rats, by gavage, for 28 days. For genotoxicity test, female Swiss mice were orally treated with 17.5, 175 or 1750 mg/kg of EEPA and the comet, micronucleus, and splenic phagocytic assays were evaluated. In subacute toxicity, the extract induced an increase in the food and water intakes, as well as in the liver absolute weight, and in the heart and kidney relative weights. EEPA also provoked alterations in histopathological analysis of liver and in hemato-biochemical parameters, evidenced by a decrease in hematocrit levels and albumin levels, and an increase in the number of platelets and in alkaline phosphatase and cholesterol levels. However, EEPA did not presented genotoxic nor mutagenic properties. EEPA showed hemato-biochemical toxicity profile in rats and should be used with caution, especially when for prolonged period.

From the leaf to the community: Distinct dimensions of phytochemical diversity shape insect–plant interactions within and among individual plants

Abstract Plant secondary chemistry is known to be an important driver of plant–insect community structure across ecological scales. Recently, the concept of phytochemical diversity (PD) has been introduced to help describe variation in plant secondary chemistry and explain how this variation affects community structure. Previous studies show that PD among individuals and species results in phytochemical mosaics, known as the phytochemical landscape. However, plant traits can vary at finer scales, such as within individuals, and even a single host plant may be perceived as an entire phytochemical landscape by an interacting insect. Using the neotropical shrub Piper amalago, we tested and compared how herbivory, caterpillar biodiversity and plant–herbivore network structure are affected by the compositional (number and concentration of compounds) and structural (diversity of distinct chemical structures) dimensions of PD. We analysed variation among individual plants and among‐plant height strata within individual plants. This allowed us to decompose PD within and among plant individuals and analyse how variation at both scales affects the plant–herbivore network. We found that both within and among plants greater structural diversity decreased herbivore feeding damage. Furthermore, each dimension of PD has different effects on herbivore biodiversity and network structure depending on the scale of biological organization. Within plants, the compositional dimension, specifically low concentrations of compounds tentatively identified as Piper amides, increased herbivore biodiversity. This dimension also increased the capacity of strata within plants to mediate ecological cascades through direct and indirect effects on herbivore abundance in the plant–herbivore network. In contrast, a greater structural diversity among plants decreased herbivore biodiversity and the capacity of plants to affect all other herbivores and plants directly and indirectly in the network. Synthesis. Based on our results we expand the concept of the phytochemical landscape to multiple scales of biological organization and provide evidence that PD may be maintained by how its multiple dimensions have distinct roles across scales of biological organization.

In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix

Piper amalago L. is a medicinal plant traditionally used as a healing agent for wounds, burns, abscesses, boils, and insect bites. The current study aimed to evaluate the possible effects of the aqueous crude extract obtained from P. amalago leaves, in different concentrations and in different incubation times, using the in vitro model of mouse fibroblasts (3T3). The extract was tested in different concentrations at the 24 h incubation time for analysis of cell viability, cytotoxicity, proliferation, cell morphology, immunostaining, adhesion and cell spreading assays, as well as to determine the hydroxyproline concentration and activity of the metalloproteinase MMP2. Morphologically, after exposure to the concentrations of 15 and 150 µg/mL, the cells maintained the morphology, yet a greater number of cells with more expansions of the cell body and larger than the control cells were observed. The treated cell culture also showed a greater number of cells, larger cells, a greater expansion of the cell body, adherent cells spread over the substrate, and a more juxtaposed, central and spherical nucleus. The treatment induced greater cell adhesion to the polymer, fibronectin, and collagen I. Biochemical results showed a significant increase in the hydroxyproline amino acid after exposure for 96 h. The extract did not induce loss of cell viability until the concentration reached 150 µg/mL, positively modulating proliferation, morphology, adhesion, degree of spreading, and organization of microfilaments. The extract also promoted a significant increase in the hydroxyproline amino acid.

The wound healing effect of aqueous extract from Piper amalago L. in diabetic patient

Diabetes patients present a complex healing process due to several factors directly linked to their pathology. The use of medicinal plants that aid in tissue repair can bring great benefits to such individuals. This case report describes how the topical application of the aqueous extract produced from the leaves of Piper amalago L. was used to aid the healing of a lacerated wound in the left thumb of a patient with type 2 diabetes mellitus.The aqueous extract of the leaves of Piper amalago L. was prepared in boiling water. During the boiling process the dried leaves were submerged in the boiling water and left for five min. The injured thumb was submerged in the solution and the leaves were placed on the injury. The action of the aqueous extract obtained from the leaves of P. amalago was shown to be promising in the healing of a wound in a patient with type 2 diabetes mellitus. The topical application of the aqueous extract produced from the leaves of P. amalago assisted in the healing of a lacerated wound in the left thumb of a patient with type 2 diabetes mellitus over a period of 15 days.

Temporal distribution in a tri-trophic system associated with Piper amalago L. in a tropical seasonal forest

Insect seasonality is a known pattern that has intrigued ecologists for over 30 years. However, despite being well understood in general, for several taxa such as Lepidopteran caterpillars its underlying causes and mechanisms are still not fully understood. This is especially critical for Brazilian tropical forests where caterpillars have previously been shown to have a puzzling pattern of peaking in abundance only in the first months of the dry season; however, this pattern still lacks an explanation. Here, to advance our understanding of the factors underlying seasonal changes in caterpillar abundance in tropical forests, we addressed how the lepidopteran caterpillar community that feeds on Piper amalago L. plants, their host plants leaf numbers, the herbivory levels, and the parasitoid pressure all change throughout the dry and wet seasons in a Brazilian tropical semideciduous forest. We found that immature abundance and herbivory peak in the first 2 months of the dry season and then rapidly decrease and remain low throughout the remaining of the dry season and the entire wet season at the study site. However, although the proportion of parasitized immatures increased alongside caterpillar abundance, it peaked in the month that followed a drastic decrease in caterpillar abundance. These results suggest that parasitoids play a major role in the observed caterpillar abundance pattern and thus, we propose the hypothesis that high parasitoid predation pressure causes early eclosion and emergence of caterpillars and primarily drives caterpillar abundance seasonality in Brazilian tropical forests.

Chemical characterisation of Piper amalago (Piperaceae) essential oil by comprehensive two-dimensional gas chromatography coupled with rapid-scanning quadrupole mass spectrometry (GC×GC/qMS) and their antilithiasic activity and acute toxicity.

Piper amalago has a distribution from Mexico to Brazil; their aerial parts have been used in folk medicine to treat diuretic and kidney diseases. The purpose of this study was to obtain a deeper understanding of the chemical composition of essential oils (EOs) extracted from both the leaves and stems of P. amalago, compare them, and evaluate their antilithiasic activity and acute toxicity. Extraction was performed by hydrodistillation, whereas chemical characterisation by two-dimensional gas chromatography coupled with rapid-scanning quadrupole mass spectrometry (GC×GC/qMS). The antilithiasic activity was evaluated by the effect of the EOs on calcium oxalate crystallisation in vitro. The turbidity index and the number of crystals formed were determined and used as an estimative of the activity. In the acute toxicity assay, the effects of a single oral dose of the EOs in Wistar rats were determined. General behaviour, adverse effects, and mortality were determined. A total of 322 compounds were identified in the EOs. The sesquiterpenes displayed the highest contribution in leaves EOs among which included bicyclogermacrene and δ-cadinene. Sesquiterpenes and oxygenated sesquiterpenes displayed the highest contribution in EOs from stems, among which included bicyclogermacrene and α-cadinol. The EOs demonstrated an excellent action on the crystals growth inhibition, and the oral dose tested did not induce significant changes in the parameters for acute toxicity. The oils have a high chemical complexity, and there are differences between their compositions, which could explain the observed differences in antilithiasic activity. The findings support the use of this plant in folk medicine to treat kidney diseases.

Supercritical Fluid Extraction of Pyrrolidine Alkaloid from Leaves of Piper amalago L.

Supercritical fluid extraction was used to extract the alkaloid N-[7-(3′,4′-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl]pyrrolidine from leaves of Piper amalago L. A three-level orthogonal array design matrix, OAD OA9(34), was used for optimization of the parameters of supercritical extraction of the alkaloid, employing supercritical carbon dioxide: extraction time (20, 40, and 60 min), temperature (40, 50, and 60°C), pressure (150, 200, and 250 bar), and the use of cosolvents (ethanol, methanol, and propyleneglycol). All parameters had significant effect on the alkaloid yield. The alkaloid yield after 60 min of extraction without cosolvents at 9 different conditions (32) in terms of temperature (40, 50, and 60°C) and pressure (150, 200, and 250 bar) was also evaluated. The optimal yield (≈3.8 mg g−1) was obtained with supercritical CO2 + methanol (5% v : v) at 40°C and 200 bar for 60 min of extraction.

USO DE ÓLEO VOLÁTIL DE <i>Piper amalago</i> L. NA ATRAÇÃO DE MORCEGOS FRUGÍVOROS

A utilização de óleos voláteis extraídos de frutos quiropterocóricos vem sendo investigada com o propósito de potencializar a chuva de sementes provocada por morcegos frugívoros em áreas degradadas. Como filostomídeos utilizam principalmente o olfato para localizar frutos maduros, teve-se, por objetivo, testar o efeito de óleos voláteis extraídos de frutos e folhas de Piper amalago L., espécie frequentemente consumida por esses animais, como atrativo para a captura. Os óleos voláteis foram extraídos por meio de aparelho Clevenger, usando-se a técnica de hidrodestilação. A eficiência do atrativo foi testada em uma área de Floresta Estacional Semidecidual no sul do Brasil. Dez redes de neblina, divididas em dois grupos, controle (sem atrativos) e teste (com atrativos), foram expostas mensalmente, de março a junho de 2012, durante três noites consecutivas. O esforço amostral correspondeu a 33.284m².h. Obteve-se 78% (39 capturas) do total de capturas para o período no grupo teste e 22% (11 capturas) no grupo controle. Os óleos voláteis de frutos e folhas de Piper amalago atraíram morcegos da espécie Sturnira lilium (E. Geoffroy, 1810), corroborando os estudos pioneiros realizados no Paraná.

ETHNOBOTANICAL STUDY IN TOLEDO-PR DISTRICT: AN APPROACH ABOUT USED SPECIES VARIABILITY WITH THE SAME POPULAR NAME

The ethnopharmacological knowledge acquired throughout human evolution culminated in the development of high therapeutic relevance drugs. In ethnobotanical survey conducted in the city of Toledo in 2007, it was found that 79% of the population often use plants for medicinal purposes, mostly in the tea form, originated from own crops. They claimed that the consumption of these plants improved their health conditions. The plants mainly cited were: balm, chamomile and marcela. This study aimed to verify if the population habits related to medicinal plants have changed, also proceed a literature review to find out if the most used plants have their biological activity scientifically proven and looking for reports of homonymous to the most cited medicinal plants. There was small change in the species used by the population. In the literature, it was found out that different species are used with the same popular name, in practice, it was detected the use of two different species, Piper amalago L. (UNOP 7972) and Piper mikanianum (Kunth) (UNOP 7971), for the medicinal plant commonly known as pariparoba, although none of them is recognized for the Brazilian Pharmacopoeia.

Anxiolytic activity and active principles of Piper amalago (Piperaceae), a medicinal plant used by the Q’eqchi’ Maya to treat susto, a culture-bound illness

Ethnopharmacological relevanceThe medicinal plant, Piper amalago L. (Piperaceae), is used traditionally by Q’eqchi’ Maya healers for the treatment of “susto” a culture-bound syndrome. Previous research suggests that susto symptoms may be a manifestation of anxiety. The objectives were to characterize the effect of ethanolic extract of P. amalago in behavioral assays of anxiety at doses representative of traditional use and to isolate active principles. Materials and methodsRats treated orally with low dose ethanolic extracts of P. amalago leaves (8–75mg/kg) were tested in several behavioral paradigms including the elevated plus maze (EPM), social interaction (SI), and conditioned emotional response (CER) tests, and compared to diazepam, a positive control. The active anxiolytic principle was isolated by bioassay guided isolation using an in vitro GABAA competitive binding assay. ResultsExtracts had significant anxiolytic activity in all behavioral tests, with the strongest activity in the SI and the CER paradigms. In an in vitro GABAA competitive binding assay, a 66.5µg/mL concentration of P. amalago ethanol extract displaced 50% of the GABAA–BZD receptor ligand [3H]-Flunitrazepam. Bioassay-guided fractionation identified a furofuran lignan, a molecule with structural similarity to yangambin, with high affinity for the GABAA-BZD receptor as the principle bioactive. ConclusionThe results suggest that the ethnobotanical use of this plant may have a pharmacological basis in its anxiolytic activity, as demonstrated in animal behaviour tests

Anti-nociceptive, anti-hyperalgesic and anti-arthritic activity of amides and extract obtained from Piper amalago in rodents

Ethnopharmacological relevancePiper amalago (Piperaceae) has been used in folk medicine as an analgesic. This study aimed to evaluate the pharmacological effects of extract and pure amides obtained from P. amalago on pain to provide a pharmacological basis for their use in traditional medicine. Aim of the studyThis study evaluated the anti-nociceptive, anti-hyperalgesic, anti-arthritic and anti-depressive activities of the ethanolic extract of P. amalago (EEPA) and the amides N-[7-(3′,4′-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl] pyrrolidine (1) and N-[7-(3′,4′-methylenedioxyphenyl)-2(E),4(E)-heptadienoyl] pyrrolidine (2) obtained from P. amalago in animal models. Materials and methodsMice treated daily with EEPA (100mg/kg, p.o.) were assayed for 20 days for knee edema (micrometer measurement), mechanical hyperalgesia (analgesiometer analysis), heat sensitivity and immobility (forced swim test) in the Complete Freund's Adjuvant (CFA) model. Cold (acetone test) and mechanical hyperalgesia (electronic von Frey analysis) responses were evaluated for 15 days in rats treated with oral EEPA (100mg/kg) in the spared nerve injury (SNI) model. Meanwhile, mice were evaluated for carrageenan-induced edema and mechanical hyperalgesia and for nociception using the formalin model after a single administration of EEPA (100mg/kg) or amides 1 and 2 (1mg/kg). ResultsAmides (1) and (2) were detected and isolated from the EEPA. The EEPA inhibited mechanical hyperalgesia, knee edema, and heat hyperalgesia, but not depressive-like behavior, induced by the intraplantar injection of CFA. When evaluated in the SNI model, the EEPA inhibited mechanical and cold hyperalgesia. The EEPA, 1 and 2 prevented the mechanical hyperalgesia induced by carrageenan and the anti-nociceptive effects in both phases of formalin nociception. The EEPA did not induce alterations in the open field test. ConclusionThe EEPA was effective for inhibition of pain and arthritic parameters but was not effective against depressive-like behavior; additionally, it did not alter locomotor activity. The amides obtained seemed to be the active component(s) present in the EEPA because they proved to be anti-nociceptive and anti-hyperalgesic in models of acute pain. Considering that few drugs are currently available for the treatment of chronic pain, especially neuropathic pain, the present results may have clinical relevance and open new possibilities for the development of new anti-hyperalgesic and anti-arthritic agents from P. amalago.

Anatomical investigations of Piper amalago (jaborandi-manso) for the quality control

Piper amalago L., Piperaceae, popularly known as jaborandi-manso, is a shrub that spans a height of 2–7m. It can be found in the regions of Southern America downward up to the south of Brazil. Traditionally it is used to treat digestive problems, heart problems, and burns. This study aims to conduct an anatomical investigation and analysis of the leaves and stems of P. amalago through electron scanning and optical micro techniques. The analysis showed that P. amalago has a hypostomatic leaf, with a subepidermal layer on its surface. There are grandular trichomes that resemble sacs, conic non-glandular trichomes, dorsiventral mesophyll, and a plano-convex midrib having a single vascular bundle in the center. The petiole is short with irregularly shaped and adaxially grooved. The stem is circular in shape and contains two circles of vascular bundles and a sclerenchymatic sheath in the perimedular region. These anatomical features of the Piper amalago's leaves and stems make it easy to pick it out among other species of the Piper genus. This is helpful when conducting quality control process.

Diuretic and antilithiasic activities of ethanolic extract from Piper amalago (Piperaceae)

ObjectivePiper amalago is used in Brazilian folk medicine as diuretic and for the treatment of urinary calculus disease, although no scientific data have been described to support these effects. Thus, this study aims to evaluate the diuretic effects and antilithiatic activity of the ethanolic extract of P. amalago (EEPam). Materials and methodsEthanolic extracts of P. amalago (125, 250 and 500mg/kg) were orally administered in male Wistar rats (n=5) and urinary excretion was measured at intervals of up to 24h after administration. The antilithiasic effect of EEPam on calcium oxalate urolithiasis crystallization was examined in a turbidimetric model. ResultsThe oral administration of all doses of EEPam significantly increased urine output after 24h when compared to control group. Moreover, the application of EEPam, induced an inhibitory effect on calcium oxalate crystallization. ConclusionsAccording to results, P. amalago extracts showed diuretic and natriuretic activity and antilithiasic effects.

Antileishmanial activity of amides from Piper amalago and synthetic analogs

Two natural amides isolated from the chloroform extract of Piper amalago L., Piperaceae, leaves, a hydrogenated derivative and seven synthetic analogs were tested against the promastigote and intracellular amastigote forms of Leishmania amazonensis. The antileishmanial activity was evaluated in terms of growth inhibitory concentration for 50% of protozoa (IC50). The cytotoxicity toward the J774A1 macrophages was evaluated in terms of the cytotoxic concentrations for 50% of macrophages (CC50). The ability to induce nitric oxide production was also investigated for all compounds. The saturated amide 7-(1,3-benzodioxol-5-yl)-1-(1-pyrrolidinyl)- 1-heptanone was obtained by hydrogenation of the natural compound N-[7-(3’,4’- methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl]pyrrolidine. Synthetic amides were prepared by addition of the appropriate amine to the corresponding acyl chloride. The natural compound, N-[7-(3’,4’-methylenedioxyphenyl)-2(E),4(E)-heptadienoyl] pyrrolidine, was the most active of all tested compounds against the promastigote and intracellular amastigote forms with IC50 values of 15μM and 14.5μM, respectively. None of the compounds modulated the production of nitric oxide.

Identification of the Volatile Compounds of Leaf, Flower, Root and Stem Oils of Piper amalago (Piperaceae)

The leaves, flowers, roots and stems of the ornamental and medicinal plant Piper amalago (Piperaceae) were collected from Dourados-MS in the Brazilian and their essential oils were analyzed by GC and GC-MS. Fifty-two, twenty-eight, forty-eight and fifty-one components were identified in the leaf, flower, root and stem oils of P. amalago, respectively. In essential oil leaves the major constituents were α- amorphene (25.7 %), p-cymene (9.4 %) and (E)-methyl geranate (7.8 %), in flowers were germacrene D (18.5 %), silphiperfol-6-ene (13.5 %) and limonene (10.5 %), in stems were α-amorphene (23.3 %), α- muurolol (9.3 %) and longifolene (6.6 %), and in roots were α-amorphene (14.4 % ), α-muurolol (6.3 %) and α-gurjunene (4.4 %).

CHEMICAL DIVERSITY OF ESSENTIAL OILS OF 15 PIPER SPECIES FROM GUATEMALA

Piper is a genus of high botanical, chemical, and pharmacological complexity. In this study, 15 species were collected and identified from two areas of the country, eight from the northern region (P. donnell-smithii, P. fallens, P. geniculatum, P. phytolaccifolium, P. psilorhachis, P. schippianum, P. sempervirens and P. variabile), and seven from the southern region (Piper amalago, P. jaquemontianum, P. oradendron, P. patulum, P. retalhuleuense, P. hispidum and P. umbellatum). From the sample, 10 species are native to Mesoamerica and the others are common to most of Latin America. Essential oils (EO) were obtained by hydrodistillation and analyzed by gas chromatography. Very high chemical diversity was demonstrated among the 15 EOs. The number of detected peaks varied from 58 (P. oradendron) to 11 (P. retahuleuense). β-caryophyllene was common to 15 oils (16.8-0.2%), β-pinene to 13 (20.5-0.2%), α-pinene to 12 (9.3-0.2%), germacrene D to 11 (18.4-0.7%), and 10 to caryophyllene oxide (27.0-1.2%), α-copaene (2.7-0.5%), β-elemene (8.0-0.2%) and γ-cadinene (8.5-0.1%). In some instances, the EO contained an abundant metabolite, being the only one with this component, such as P. jacquemontianum (linalool 70.8%), P. amalago (nerolidol, 48.7%), P. patulum (1,3,5-trimethoxy-2-propenylbenzene, 33.7%, methyl eugenol, 24.6%), P. variabile (camphor, 29.7%), P. sempervirens (bicyclogermacrene, 28.5%), P. retalhuleuense (nerolidol, 28.2%), and P. schippianum (dihydroaromadendrene, 22.1%). The high diversity of EO composition from genus Piper was confirmed, as well as the possibility for agronomical development of some of the species due to the commercial potential of some of their components, such as linalool and nerolidol.