In this paper the system based on Python programming language for control of Pioneer 3dx mobile robot is considered. To write code in Python initially the ARIA language imported. The Pioneer 3dx’s simulation control is started by creating a robot object and connecting it with the emulator. In case of failed connection Pioneer is performed exiting from the program. Program Codes is written for research works and performs some actions like grabbing and moving the ball with the external gripper, and then putting this ball on another place. This routine code is useful in performing some laboratory and industry tests. The areas, where could be used Pioneer 3dx mobile robot and method of application to the different fields are not limited. As our research shown that the Pioneer 3dx with gripper mainly can be used in industries for routine work, in laboratories for different experiments. The hardware components of mobile robot are pitman motor, motor control, sensors, actuators and communication among them. The main focus was receiving initial data from sensors. Movement is performed based on robot’s motor. The control panels located on the Pioneer robot have indicators that increase capabilities of mobile robot. The software packages used for Pioneer robot is eyes-client mobile application. Additionally, in research work the ultrasonic sensor’s working principle and logic were applied. Core programming language ARIA and its working through Python considered also. The code written during research work was applied on the simulator MobileSim that makes robot perform a series of planned actions. The application of smartphone on Pioneer 3dx robot for speech recognition purpose can improve the functionality and effectiveness of it. Moreover, the additional camera also underlined as new feature for security and monitoring system.