Liquid Pineapple Research Articles

Impact of Simulated Human Gastrointestinal Digestion on the Bioactive Fraction of Upcycled Pineapple By-Products.

Pineapple by-products (peels and stems) from fruit processing industries were evaluated to understand its potential application as a functional food. Therefore, the bioactive compounds of pineapple by-products were characterized for prebiotic and antioxidant activities. A total characterization of soluble carbohydrates profile (simples and complex carbohydrates), as well as polyphenols was performed, after removal of enzymatic fraction from pineapple crude juice, allowing the decrease of proteolytic activity and improving the other biological activities. Results showed that pineapple liquid fraction, from stem and peels, can be applied as a prebiotic enhancer, promoting the growth of five probiotic microorganisms (two strains of Lactobacillus sp. and three strains of Bifidobacterium sp.), as a single carbohydrate source. Moreover, through HPLC (High Performance Liquid Chromatography) analysis, 10 polyphenols were identified in pineapple liquid fractions, with some expected differences between both evaluated by-products. Gastrointestinal tract was simulated, in a continuous mode to understand the impact of pH changes and gastrointestinal enzymes into pineapple liquid fractions. Results showed a digestion of high molecular weight polysaccharides into small molecular weight tri-, di-, and monosaccharides. There was an increase of samples antioxidant activity through the gastrointestinal stage, followed by the release of specific polyphenols, such as chlorogenic, coumaric, and ferulic acids. The prebiotic activity did not improve throughout the simulation, in fact, the prebiotic potential decreased throughout the different stages.

Optimization of Hydraulic Retention Time and Organic Loading Rate in Anaerobic Digestion of Squeezed Pineapple Liquid Wastes for Biogas Production

Pineapple wastes are produced in huge amount during the industrial canning process of pineapple; in Thailand over 400,000 tons per annum of canned pineapple exported leaving behind the waste. Besides the pulps and peels as solid wastes, the squeezed pineapple liquid wastes (SPLW) extracted from solid wastes can also be used for anaerobic digestion. In the present study, the anaerobic digestion of liquid squeezed from industrial pineapple peels was carried out using a lab-scale hybrid reactor. The reactor was operated for over 170 days with the hydraulic retention time (HRT) of 20 days decreasing down to 5 days and simultaneous control of organic loading rate (OLR). Under controlled conditions in the hybrid reactor, pH was maintained at 6.5–7.6 by adding alkaline for anaerobic microbial activity. Results showed that the chemical oxygen demand (COD) removal efficiency was at ≥ 90% for all conditions. The biogas production (mL/day) increased thoroughly from longer HRT to shorter HRT, as same as methane production with the maximum values (HRT 5 days, OLR 5 g/COD/ day with recirculation) of 55,130 and 30,322 mL/day, respectively. Moreover, the highest yields of biogas and methane were also investigated under similar conditions with the values of 0.504 and 0.277 L/gCOD, respectively. Interestingly, this optimization of both HRT and OLR of lab-scale anaerobic digestion process could be further practically applied to pilot or industrial scale in canned pineapple factories for biogas production.

PENGARUH VARIASI DOSIS PUPUK ORGANIK LIMBAH CAIR NANAS (LCN) TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN SELADA

Abstract: This research is an experimental research, which aims to determine the effect of variation dosage in of organic pineapple liquid waste fertilizer, on the growth and productivity of red lettuce (Lactuca Sativa L) and its potential as learning resources of biology learning. This research uses a Completely Randomized Design with 1 control and 3 treatment combinations and 5 repetitions. This research was conducted for 30 days. The parameters which is used in this research include plant height and wet weight of lettuce (Lactuca sativa L), with supporting data in the form of number of leaves and stem diameter. The main data is tabulated and analysed using the SPSS 25.0 application which includes Multivariate Analysis of Variance. The results of this research can be concluded that the application of organic fertillizer from pineaplle liquid waste has a significant influence on the growth and product of lettuce (Lactuca sativa L). The results of the research be used as a practical guide on plant growth and development materials.

Pumakkal compost formula for degrading shrimp pond sediment (As a learning source of Biology subject)

Research used completely randomized design (CRD) 5 treatments of C3 (3 bacteria), C6 (6 bacteria), C9 (9 bacteria), C12 (12 bacteria) and C15 (15 bacteria) 5 replications. Compost quality is measured activated carbon, Nitrogen (N), C / N ratio, levels of phosphorus (P), calcium (Ca) and potassium (K). A total of 30 samples weighing 300 g each after 30 days of fermentation was analyzed in the Chemistry Laboratory of the University of Muhammadiyah Malang. The results showed that the five treatments were significantly different. Pumakkal Pineapple Liquid Waste Bioremediator is able to degrade shrimp pond sediments into Shrimp pond sediment compost (KSTU), from the parameters of organic carbon, C / N ratio, nitrogen, phosphorus, potassium and the degree of acidity (pH). The most effective treatment is Konsorsia C15 (15 isolates) with the best average yield. Treatment of C15 consortia (15 bacteria) obtained 25% C-organic yield, for a C/N ratio of 24; Nitrogen (N) 3.4%, Pospor 2.3%, from Potassium 2.1%, Calcium (Ca) content 20%and pH 7.2

Organic fertilizer of coffee peel with PUMAKKAL starter formula for sustainable plantation cultivation

Coffee is a strategic commodity that generates promising foreign exchange, where Lampung is the main coffee production centre which continues to increase its production, and more than 80% of it is smallholder coffee. Between 40% and 55% of coffee, production is coffee husk waste, which has not been optimally utilized. Previous research showed that pineapple liquid waste indigen bacteria (Pumakkal) were able to increase acidity and decompose waste organic matter, and the final result was a suitable fertilizer for plant cultivation. This research will solve the problem of coffee husk waste used as compost by utilizing a starter from the previously tested pineapple liquid waste bacteria (Pumakkal). The research was conducted with the first stage of making coffee skin compost with a starter formula of KA (3 bacteria), KB (6 bacteria), KC (9 bacteria), KD (12 bacteria) and KE (15 bacteria), the second stage was to test the quality of the coffee husk compost organic fertilizer followed by a pilot plan application using the best formula. The application uses spinach and chilli plants. The result shows that KC is a model the most appropriate coffee skin compost starter formula.

The The Effect of Acetobacter xylinum Starter in Waste Liquid Pineapple Peel on the Properties of Nata de Pina

Abstract. Fresh pineapple waste consisted of saccharides, water, protein, lipid, vitamin and minerals that can be used by beneficiary microorganisms for production another food products. The aim of this study was to investigate physical properties nata de pina made from waste liquid pineapple peel using various starter concentrations and length of fermentation of Acetobacter xylinum. The starter used were 20, 30 and 40% ml. Each 200 mL fresh waste containing the starter was incubated for 14, 21 and 28 days at 29°C. Nata properties such as yield, thickness, fibre and water content were determined. Results showed starter concentration and length of fermentation affect and significantly (P<0.05) different on nata thickness, yield and fiber content. The highest yield (35,75%), thickness (24.6 mm) and fibre content (4.43%) occurred at 40% starter after 28 days of fermentation. However, the optimum nata thickness occurred at 21 days. There is no significantly different (P<0.05) at nata moisture on starter concentration and length of fermentation. In conclusion, waste liquid pineapple peel is potential as a raw material source for making nata de pina with starter Acetobacter xylinum.

PENGARUH PGPR AKAR BAMBU APUS DAN PUPUK LIMBAH CAIR NANAS TERHADAP PERTUMBUHAN BAWANG DAUN UNTUK INFORMASI MASYARAKAT

Increasing agricultural crop production, especially onion, can be done by improving the optimal growing environment for plants. Plant roots can be optimized for the absorption of nutrients and water in the process of photosynthesis. One way that can be used is by applying Plant Growth Promoting Rhizobacteria (PGPR). In addition to increasing the content of micro and macro elements in the soil can also support the growth of leek plants obtained by applying Pineapple Liquid Waste (LCN) made using bacterial isolation and can meet the needs of nutrients in plants. This study aims to determine the effect Plant Growth Promoting Rhizobacteria (PGPR) from Apus (Gigantochola apus) bamboo root and Pineapple Liquid Waste (LCN) fertilizer on the growth of leeks (Allium fistulosum L.), then the results of this study are treated as community information in the form of Leaflets. The method used in this study was an experiment using a Completely Randomized Design (CRD) system consisting of 4x4 factorials with 3 replications. Based on the results of calculations and data analysis, the results show that there is an effect of PGPR and LCN on the growth of scallions on the height and number of tillers per scallion plant family, but there is no influence on pseudo stem circumference and wet weight per scallion plant family, and the results of this study can be used as a source of public information in the form of leaflets with an average percentage included in good eligibility criteria.

Application pineapple liquid waste to increase fruit weight and vitamin c pineapple as biological learning resources

Pineapple Liquid Waste (PLW) is waste that has acidity characteristics and contains organic material which can be utilized as a substrate for bacterial growth. Researchers are trying to provide the latest breakthroughs to utilize Pineapple Waste (PLW) as organic fertilizer to reduce waste increases and increase agricultural yields. The purpose of this research is to find out the effect on the weight of pineapple and find out whether it will increase vitamin C levels in pineapple. This study used an Experimental Method, the design of the design used was a Complete Random Design Variety and analyzed using a multivariate test. the conclusion of this study is the variation of PLW has a significant effect on fruit weight p> 0.05 and on vitamin C levels p < 0.05, so that no significant effect on fruit weight and significant effect on increasing levels of vitamin C, so this research can potentially design learning resources for biology.

The use of pineapple liquid waste and cow dung compost to improve the availability of soil N, P, and K and growth of pineapple plant in an Ultisol of Central Lampung

A study that was aimed to elucidate the effect of adding pineapple liquid waste and cow dung compost in increasing the availability of soil N, P, K, contents of N, P, K in pineapple leaves and growth of pineapple plant in an Ultisol was conducted in Central Lampung. The study consisted of two steps, i.e. incubation of pineapple liquid waste with cow dung compost in the soil, and planting pineapple by adding pineapple liquid waste and cow dung compost. Treatments tested in experiment 1 (laboratory incubation experiment) were combinations of pineapple liquid waste (L) and cow dung compost (K). Changes of soil chemical characteristics were observed at 15, 30, 45, and 60 days. Three best treatments of experiment 1 were selected for the experiment 2 (plant growth experiment in a glasshouse). Two control treatments were included in experiment 2. N, P, and K contents in pineapple leaves as well as and pineapple root length were observed at 30 and 60 days. Results of the first experiment showed that addition of pineapple liquid waste and cow dung compost significantly increased soil pH, availability of soil N, P, and K compared to controls at 15, 30, 45, and 60 days. Addition of 20 t cow dung compost/ha and 20,000 L pineapple liquid waste/ha (K3L3 treatment) resulted in higher values of soil pH, total N, available P, and available K than the control. Results of the second experiment showed that the addition of pineapple liquid waste and cow dung compost gave no significant effect on the growth of pineapple plant, but the addition of 20 t cow dung compost/ha and 10,000 L pineapple liquid waste/ha (K3L2 treatment) gave optimum growth compared to other treatments at 60 days. The K3L2 treatment also had higher pineapple leaf nutrient content, compared to control.

Gelatin extraction from the indigenous Pangasius catfish bone using pineapple liquid waste

Gelatin extraction from fish bone has traditionally involved hydrogen chloride and/or sodium hydroxide during pre-treatment. However, these chemicals have begun to be abandoned because of their associated safety and environmental issues. Several studies have looked at the use of citric acid as a safer alternative in fish bone gelatin extraction. The aim of this research was to extract gelatin from the bone of Pangasius catfish with pineapple liquid waste. The extraction was performed in two steps: pre-treatment followed by main extraction at various times (24–56 h) and temperatures (45–75°C). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used as a confirmation test and showed a band for gelatin at ~120 kDa. Gelatin yields were calculated as the ratio of weight of dried gelatin to the total weight of fish ossein. The results indicated that pineapple liquid waste can be used for fish bone gelatin extraction. The recommended conditions for extraction of fish bone gelatin using pineapple liquid waste are 56 h of pre-treatment and 5 h of main extraction at a temperature of 75°C. The gelatin yield was 6.12% and the protein concentration 4.00 g/100 g.

Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

Solid And Liquid Pineapple Waste Utilization For Lactic Acid Fermentation

The liquid and solid pineapple waste contain mainly sucrose, glucose,fructose, and other nutrients. It therefore can potentially be used as carbon source for fermentation to produce organic acid. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer, the experiments were carried out in batch fermentation using the liquid and solid pineapple waste to produce lactic acid. The anaerobic fermentation of lactic acis were performed at 40 0C, ph 6, 5% inocolum, and 50 rpm. Initially results show that the liquid pineapple waste by using Lactobacillus delbrueckii can be used as carbon source for lactic acid fermentation. The production of lactic acid are found to be 79% yield, while only 56% yield was produced y using solid waste.Keywords : Lactic acid fermentation, Pineapple waste, Lactobacillus delbrueckii

PERBANDINGAN JENIS MEDIA TANAM TANAH ENTISOL YANG TERPAPAR SATU KALI DAN DUA KALI OLEH LIMBAH CAIR NANAS TERHADAP PERTUMBUHAN SELEDRI (Apium graveolens L.) SEBAGAI SUMBER BELAJAR BIOLOGI

Abstract. This research to know effect of Pineapple Liquid Compost (PLC) in long term the growth of celery. This research conducted at the village in Yosodadi 21 Polos, East Metro, Metro City since 7 December until 28 February 2015. This research is experimental research, the design used the Complete Random Design (CRD). This research used entisol soil that was exposed by PLC once and entisol soil that was exposed by PLC twice. The treatment was done to them both by giving PLC about 200ml in a time per three days. The data analysis was done by using normality test (liliefors), homogeneity test (barlett) and Ttes. It shows that there is no negative effect of fertilizer use PLC in the long term against the growth of celery in entisol soil that was exposed by PLC once and entisol soil that was exposed by PLC twice.

Characterization of Liquid Pineapple Waste as Carbon Source for Production of Succinic Acid

Pineapple cannery produces large amount of solid and liquid waste. The disposal of waste without an appropriate treatment can cause a great environmental pollution. Since pineapple waste contains some valuable components such as glucose, fructose and sucrose, the ability to convert this waste into higher value added product such as succinic acid would be advantageous. Therefore, in this study, liquid pineapple waste was characterized in order to investigate the possibility of succinic acid production via fermentation using liquid pineapple waste as a carbon source. The physical and chemical composition in the liquid pineapple waste such as cation, anion, pH, sugar content and soluble protein were determined. The dominant sugar in the liquid pineapple waste were glucose, fructose and sucrose and the total sugar content was more than 100 g/l. Result from the fermentation process proved that liquid pineapple waste can successfully produce succinic acid with almost the same amount as using glucose as carbon source, with the concentration of 6.26 g/l.

Response surface optimization of glucose production from liquid pineapple waste using immobilized invertase in PVA–alginate–sulfate beads

Pineapple waste is known as a byproduct of the pineapple processing industry and typically consists of residual pulp, peels and skin. In this study, the conversion of sucrose to glucose was performed with the help of Baker’s yeast invertase. Response Surface Methodology (RSM) comprising Box–Behnken design was successfully applied in this study as a tool to evaluate the interactive effects and to obtain the optimum operating conditions for the enzymatic hydrolysis. Four parameters were varied: pH (4–6), temperature (40–60°C), agitation rate (100–200rpm) and amount of immobilized beads (3–6g). Results revealed that the optimum operating conditions derived via RSM were: pH 4.74, temperature 50.11°C, agitation rate of 147.68rpm and amount of immobilized beads used were 4.45g. The experimental yield of glucose was found to be 111.42g/L (850.5%) and sucrose 25.70g/L (32.3%) under the optimum conditions, which correlated well with the maximum predicted value of glucose and sucrose 110.13g/L (840.7%) and 25.93g/L (32.6%) respectively. Hydrolysis of the liquid pineapple waste showed that 95% of sucrose content in the waste was hydrolyzed to glucose after 3h of hydrolysis and the overall glucose generated from the hydrolysis was approximately 1131% of its original content.

Process Parameters for Fermentation in a Rotary Disc Reactor for Optimum Microbial Cellulose Production using Response Surface Methodology

In this study, microbial cellulose production by Acetobacter xylinum 0416 using standardized liquid pineapple waste was carried out in a 4-L rotary disc reactor (RDR). The objective of this study was to optimize the process parameters for production of microbial cellulose in the RDR. The effects of the disc rotation speed (5 to 12 rpm), pH (3.5 to 7.5), fermentation period (3 to 6 days), and inoculum concentration (3 to 20% v/v) on the microbial cellulose production were investigated. The optimum microbial cellulose yield was obtained using 10% (v/v) of inoculum concentration, whereby four days’ duration gave the most productive yield. In addition, the highest production of microbial cellulose was obtained at a low disc rotation speed of 7 rpm and a pH of 5.0. Analysis of data performed a high coefficient of determination value (R2=0.875) represented by a mathematical model of optimized microbial cellulose production, Y = -200.437 + 7.180X1 + 69.869X2 + 4.369X3 + 1.867X4 – 0.512X12 – 6.766X22 – 0.585X32 – 0.079X42. From the results, it can be concluded that the foremost factors that affect the production of microbial cellulose in RDR were pH followed by inoculum concentration, disc rotation speed (rpm), and fermentation period.

PINEAPPLE LIQUID WASTE AS NATA DE PINA RAW MATERIAL

This research aims to study the quantity, quality, ecological and economic feasibility of nata de pina production (NP) from pineapple liquid waste (PLW). The design of the study employs complete random design (CRD) with three treatments: PLW without nutrients addition (A), PLW nutrients addition (B), and PLW stored for six months with nutrients addition (C). The nata de pina’s production factors measured were weight, thickness, fiber content, color, brightness, and residual waste. The highest weight was reached in treatment B (899 grams), followed by treatment A (616.4 grams), and C (477.8 grams). The thickness of NP of the height and low as in treatment B (1.58 cm) followed by treatment A (1.24 cm) and C (0.88 cm), respectively. The highest fiber content was found in treatment C (9.3%) followed by treatment B (7.6%) and A (6.9%), respectively. The fiber content, along with color quality and brightness fit with food standards. The production of NP may reduce the volume of the PLW from 46.2% to 89.1% ( p= 0.001). Based on the standard value of biological oxygen demand (BOD), chemical oxygen demand (COD), total suspended solid (TSS) below to the required threshold except pH. The production of NP is economically feasible to 4.7 BC ratio. The overal manufacture of nata de pina from PLW produces better and feasible product ecologically and economically

DEGRADASI BAHAN ORGANIK LIMBAH CAIR NANAS OLEH BAKTERI INDIGEN

Pineapple Liquid Waste has high organic material and still unfulfilled required quality standard. Processing occurred at the waste processing installation with pond system (Lagoon) required a wide space and keep long time so that less efficient. Based on this problem, it needs an environmentally sound waste management by utilizing bioprocess technology using indigenous bacteria’s ability in degrading organic pollutant called Bioremediation. The objective of this research was to get isolated indigenous bacteria, enables to decrease organic material. It was divided into four stages. First, performed characteristic observation of isolated bacteria that potentially decrease organic material, secondly decomposition capability test in vitro, and thirdly scale bioremediation test pilot plan, fourthly bioremediation test in ex situ at the microbiology laboratory of Malang State University. Data were analyzed by Varian analysis to test the presence or absence of differences and regression test to analyze the link between variables. First phase result: 15 isolated indigenous bacteria.. Potential consortia bacteria consisting of more effective 4 species, indicated by 4 consortia bacteria decreased BOD in 6 days. Starter bacteria with a consortia K1’s combination (ABCD), Volume: 5% (v/v) and 6 days were the most effective incubation time in decreasing BOD. Second phase result: 1) A significant link among the number of bacteria, a. Bacillus cereus, b. Acinetobacter baumanni, c.Bacillus subtilis, d. Pseudomonas pseudomallei and not abcd against organic material with contribution of 72,7% and negatively correlated, 2) Media treatment and incubation time gave distinctive outcome against organic material, 3) there was a link between organic material organic acid concentration decrease would decrease BOD at scale bioremediation test Pilot plan, and ex situ scale. Threshold BOD achievement limit value on 10th day (75) quality standard: 75. In accordance with ministry decree LH No. 05 year 2007 industrial wastewater quality standard, suggested to industry to use this study in processing its waste.&lt;br /&gt;&lt;br /&gt;Keywords: organic material, isolated indigenous bacteria, pineapple liquid waste.&lt;br /&gt;&lt;br /&gt;

PRODUCTION OF PIGMENTS FROM BACTERIA GROWN IN SOLID AND LIQUID PINEAPPLE WASTE

Awareness of the use of natural pigments has escalated over the years due to stringent environmental standards imposed on synthetic pigments. Natural pigments can be extracted from microorganisms, notably bacteria. However, the high cost of the growth medium hampers their large scale production. We report the extraction of two pigments, violacein and prodigiosin from Chromobacterium violaceum and Serratia marcescens, respectively, grown in liquid pineapple and solid pineapple waste. The pigments were succesfully applied for dyeing of natural and synthetic fabrics.

Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix

A thorough investigation of sucrose hydrolysis by invertase immobilized in recently developed PVA–alginate beads was performed. The developed PVA–alginate bead is distinguished by the treatment with sodium sulfate solution. The performance of invertase immobilized in PVA–alginate matrix beads in terms of leakage, reusability, ideal temperature and pH and kinetic parameters K m and V max was investigated. Results revealed that the ideal temperature and pH for the immobilized invertase are 50 °C and 4 respectively. The apparent K m of each bead is relatively lower than that of the free enzyme with the value of 4.7 × 10 −5 mM and 2.849 × 10 −5 mM for 12PVA–5BA and 12PVA–7BA, respectively. An increase in V max was also observed with the value of 2281.02 U mg −1 enzyme and 2281.02 U mg −1 enzyme for 12PVA–5BA and 12PVA–7BA, respectively. The reusability of the beads and its storage period were also investigated and were found to be 14 cycles and 60 days, respectively. Finally, the beads with the best performance (12PVA–5BA) were used to hydrolyze liquid pineapple waste. The production yield of glucose was about 745% in 3 h in the hydrolysis of the liquid pineapple waste.