Pine Oil Research Articles

Study of PCCI engine operating on pine oil diesel blend (P50) with benzyl alcohol and diethyl ether

This article examines the effect of premixing of benzyl alcohol and Diethyl Ether (DEE) in a CI engine fuelled with pine oil diesel fuel blend. The fuel sample is prepared by blending 50% of pine oil with 50% of diesel fuel (P50) on volume basics. The benzyl alcohol and diethyl ether were injected in the premixing ratio of 10% and 20% on the CI engine fuelled with P50. The experimental analysis was conducted in a 4-stroke single cylinder CI engine with added provision to operate on PCCI mode. The engine was operated with constant speed and tested at different torque condition ranging from 0 to 100 % in the interval of 25%. The experiment results shows that premixing of benzyl alcohol and diethyl ether enhanced the brake thermal efficiency by 4.5 % and 8.75 % than P50 respectively and brake specific energy consumptions was low when compared to neat P50 operation. Premixing of diethyl ether and benzyl alcohol increased the emission of CO and HC whereas reduced smoke and NOx emission. There was increase in peak combustion pressure and heat release rate with the premixing of benzyl alcohol however, premixing of diethyl ether resulted in reverse trend.

Synthesis, Characterization, and Comparison of Disinfectant Bioactivity Test of Two Triphenyltin(IV) Compounds

This paper aims to report the synthesis of two new organotin(IV) carboxylate derivatives, triphenyltin(IV) 4-aminobenzoate (2) and triphenyltin(IV) 4-nitrobenzoate (3) and to examine their antibacterial activity as a disinfectant. These compounds were prepared by reacting triphenyltin(IV) hydroxide (1) with 4-aminobenzoic acid and 4-nitrobenzoic acid, respectively. Compound (2) was obtained as a yellow solid with a yield of 84.09% and compound (3) in the form of a white solid with a yield of 80.70%. These compounds were well characterized using UV-Vis spectrometry, FT-IR spectrometry and NMR spectroscopy. The bioactivity test as a disinfectant was tested against Salmonella typhosa and Staphylococcus aureus. The activity test was carried out by measuring the optical density (OD) of the tested compounds with concentration variations of 5x10-3, 1x10-3, and 5x10-4 M in methanol and 5% dimethyl sulfoxide (DMSO), commercial Wipol (2.5% pine oil) was used as a positive control with observations monitored at contact times of 0, 5, 10, and 15 minutes. The results showed that of both compounds were active against the two bacteria compared to the positive control with compound 3 found to be more active than compound 2.

Disinvasive activity of “Hemastal BIO” and “Staldren” against non-invasive eggs of Trichuris skrjabini nematodes parasitizing sheep

Geohelminths parasitizing sheep are widespread throughout the world and have a significant pathogenic effect on the animal body. Among them, one of the leading places in terms of distribution is occupied by helminths of the genus Trichuris. This is due to the high resistance of trichurises to the effects of chemicals and adverse environmental factors. The presence of viable eggs of trichurosis pathogens in the surface layer of the soil and a sufficiently long period of their storage in the environment represents a potential threat of parasitic invasion on the territory of sheep farms. The aim of the research was to determine the disinfestation activity of modern ecologically safe means for dry disinfection against eggs of non-invasive nematode eggs of Trichuris skrjabini parasitizing sheep. An experimental test of the preparations “Hemastal BIO” (AS – calcium hydroxide, calcium sulfate, calcium sulfite-semihydrate, calcium chloride) and “Staldren” (AS – calcium carbonate, pine oil, magnesium silicate, iron oxide) was carried out in laboratory conditions. In the experiment, test cultures of eggs isolated from the gonads of T. skrjabini nematode females were used. The results of the conducted research established a high level of disinfestation efficiency of “Hemastal BIO” and “Staldren” (DE – 100 %) relative to non-invasive eggs of T. skrjabini on the 54th day of cultivation – the period of formation of invasive eggs in the control culture. During the embryogenesis of trichurises, the effectiveness of dry disinfectants gradually increased with “Hemastal BIO” up to 88.84 % – on the 18th day and up to 98.36 % – on the 36th day, and “Staldren” up to 82.79 % – on the 18th day and up to 95.90 % – for 36th days. Their harmful effect was characterized by specific changes in the eggs of nematodes in the form of sticking of crystals of the agents around the causative agents, as a result of which there was a stop in growth and development and the gradual decay and resorption of the embryos. Such changes are also confirmed by the metric parameters of the width and length of T. skrjabini eggs. The obtained data allow to recommend dry disinfectants “Hemastal BIO” and “Staldren” for the purpose of effective maintenance of sanitary and epidemiological well-being in relation to trichurosis in sheep farms.

Newly Discovered Components of Dendrolimus pini Sex Pheromone.

The pine-tree lappet moth, D. pini, is a harmful defoliator of pine forests in Europe and Asia and a potentially invasive species in North America. The lures for trapping D. pini males based on two known components of its sex pheromone appeared weakly attractive to male moths. Identification of all components of the sex pheromone might allow for the development of more effective lures. The pheromone was sampled from virgin females using SPME and analyzed using gas chromatography coupled with mass spectrometry. Four new likely components ((Z5)-dodecenal, (Z5)-dodecen-1-ol, (Z5)-decen-1-yl acetate, (Z5)-tetradecen-1-yl acetate) and two known components ((Z5,E7)-dodecadienal, (Z5,E7)-dodecadien-1-ol) were identified based on comparison against authentic standards, Kováts indices and spectra libraries. The samples also contained several sesquiterpenes. Wind tunnel and field experiments showed that some blends of synthetic pheromone components alone or enriched with Scots pine essential oil (SPEO) were attractive to D. pini males. One component-(Z5)-decen-1-yl acetate-had a repelling effect. The presented knowledge of D. pini sex pheromone provides a basis for developing optimal lures for monitoring or controlling insect populations.

Essential Oils as Antimicrobial Active Substances in Wound Dressings.

Wound dressings for skin lesions, such as bedsores or pressure ulcers, are widely used for many patients, both during hospitalization and in subsequent treatment at home. To improve the treatment and shorten the healing time and, therefore, the cost, numerous types of wound dressings have been developed by manufacturers. Considering certain inconveniences related to the intolerance of some patients to antibiotics and the antimicrobial, antioxidant, and curative properties of certain essential oils, we conducted research by incorporating these oils, based on polyvinyl alcohol/ polyvinyl pyrrolidone (PVA/PVP) biopolymers, into dressings. The objective of this study was to study the potential of a polymeric matrix for wound healing, with polyvinyl alcohol as the main material and polyvinyl pyrrolidone and hydroxypropyl methylcellulose (HPMC) as secondary materials, together with additives (plasticizers poly(ethylene glycol) (PEG) and glycerol), stabilizers (Zn stearate), antioxidants (vitamin A and vitamin E), and four types of essential oils (fennel, peppermint, pine, and thyme essential oils). For all the studied samples, the combining compatibility, antimicrobial, and cytotoxicity properties were investigated. The obtained results demonstrated a uniform morphology for almost all the samples and adequate barrier properties for contact with suppurating wounds. The results show that the obtained samples containing essential oils have a good inhibitory effect on, or antimicrobial properties against, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231. The MTT assay showed that the tested samples were not toxic and did not lead to cell death. The results showed that the essential oils used provide an effective solution as active substances in wound dressings.

Investigation of the production of manganese nanomaterials from fine and low-grade manganese ore by two-stage column flotation technique

ABSTRACT This study was carried out to investigate the production of manganese nanomaterials by enriching fine and low-grade manganese ores. Fine and low-grade manganese ore sample was obtained from Balıkesir-Mezit-Tumanpınarı region in Turkey. Experimental studies were processed in two different routes. In the first method, optimum enrichment conditions were determined by enriching the ore with a two-stage column flotation method. The optimum flotation time obtained for fine and low-grade manganese ore is 10 minutes. The optimum variables obtained from 10 min column flotation are 60 g/dm3 collector (sodium oleate), 1 g/dm3 depressant (sodium silicate), 3 g/dm3 frother (pine oil) and pH 7 (natural pH of manganese ore). Higher-grade MnO2 was obtained at the natural pH value of manganese ore. Under the optimum conditions, the concentrate of 76.55% MnO2 could be recovered by two-stage column flotation from the ore containing 29.9% MnO with the recovery of 90.25%. In the other route, the enriched sample was dissolved and MnCO3 was obtained by using Na2CO3. Then, 400 nm manganese oxide nanorods were produced by roasting MnCO3.

Effective purification of graphite via low pulp density flotation-low temperature alkali roasting-acid leaching route: From laboratory-scale to pilot-scale

A typical flake graphite concentrate sampled from a graphite flotation plant was used to prepare high purity graphite. The carbon content of the concentrate was 85.6%, and silicate and iron-containing minerals were found principally in the concentrate. High-grade graphite concentrate with a carbon content of 95% was obtained in the flotation process with feed pulp of 5% solids by weight after regrinding by a low-speed ball milling system. A small amount of the flotation reagents such as caustic soda, water glass, kerosene, and pine oil were added. The resulting flotation concentrate was mixed with alkali and roasted at 500 °C for 90 min. During the alkali roasting, the silicate mineral was converted into the water- and acid-soluble materials. Afterward, the soluble materials and iron-containing minerals were dissolved by acid leaching. As a result, a product with a carbon content of 99.82% was prepared. According to the calculation results of Gibbs energy, the reactions between impurities and chemicals in alkali roasting and acid leaching processes were spontaneous. The purification route was very effective for high purity graphite production and has been successfully applied in the pilot-scale experiment.

In Vitro Antibacterial Activity of Some Plant Essential Oils against Four Different Microbial Strains

This study evaluates the antimicrobial and antioxidant activities of five essential oils (EO): pine oil, thyme oil, sage oil, fennel oil, and eucalyptus essential oils. To identify the chemical composition of the essential oils, we used gas chromatography coupled to a mass spectrometer (GC-MS). EO are predominantly characterized by the presence of monoterpene hydrocarbons and oxygenated monoterpenes, except in the case of fennel essential oil which contains phenylpropanoids as its main components. The antimicrobial activity of the EO was highlighted on four standard microbial strains (two Gram-negative strains-Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853; one Gram-positive strain Staphylococcus aureus ATCC 25923, and one yeast strain-Candida albicans ATCC 10231). Antimicrobial activity was assessed by measuring the diameter of the inhibition zone, and by determining the values of the minimum inhibitory concentration (MIC) and minimum concentration of biofilm eradication (MCBE). Analyzing the diameter values of the inhibition zones we observed increased efficiency of thyme essential oil, which showed the highest values for all tested microbial species. The results of tests performed in a liquid confirm the high sensitivity of the standard strain Escherichia coli ATCC 25922 to the action of all essential oils, the lowest values of MIC being recorded for sage and thyme essential oils. For the most essential oils tested in this study, the MCBE values are close to the MIC values, except for the pine EO which seems to have stimulated the adhesion of the yeast strain at concentrations lower than 5%. The study highlights the antimicrobial activity of the tested essential oils on Gram-positive and Gram-negative strains.

Produced water treatment using microemulsion formulated with vegetable oil-based surfactant

The presence of produced water is common in oil production. In certain production stages, the volume of produced water exceeds that of oil. Pretreatment is necessary before the disposal or reinjection of produced water into wells because it is harmful to the environment owing to the high oil and grease contents (OGC), salinity, and other factors. This study proposes a produced water treatment using microemulsions (ME) with vegetable oil-based surfactants (S), with the aim of reducing two main factors, OGC and salinity. The decision to use a vegetable oil-based surfactant as an alternative to a petroleum-based surfactant was made to reduce the negative effects of the overall process on the environment. Coconut soap, the surfactant used in the formulated ME, was selected after testing a variety of vegetable oil-based surfactants. The other components used in the ME formulation were 2-butanol as a co-surfactant (C), pine oil as the oil phase, and saline water at 2 % NaCl as the water phase. The ME point used in the water treatment was defined by D-optimal mixture planning, composed of 85 % C/S, 2.5 % oil phase, and 12.5 % water phase, with a C/S ratio of 10. In addition, we used a complete factorial experimental design with combinations of three variables (time, temperature, and microemulsion percentage), and repetition of the central point to select the greatest reduction in OGC and salinity. According to the results, OGC reduction values ranged from 89.15 % to 95.43 %, with the real OGC values ranging from 16.98 mg·L−1 to 7.15 mg·L−1. These values are within the limits established by environmental agencies, CONAMA, US EPA, and the OSPAR Commission, for the disposal of produced water in the sea. In addition, salinity reduction varied from 38.02 % to 46.8 %, with real salinity decreasing from 36,223.53 mg·L−1 to 31,217.65 mg·L−1. Therefore, it can be said that using a vegetable oil-based and biodegradable surfactant in the microemulsion formulation for the treatment of produced water is as effective in the reduction of OGC and salinity as petroleum-based surfactants, which are more harmful to the environment.

Essential Oil Compositions of Pinus Species (P. contorta Subsp. contorta, P. ponderosa var. ponderosa, and P. flexilis); Enantiomeric Distribution of Terpenoids in Pinus Species.

Pinus species are important in traditional medicine throughout their ranges, and pine essential oils are of interest in aromatherapy and as topical treatments. In this work, the leaf (needle) essential oils of Pinus ponderosa var. ponderosa and Pinus contorta subsp. contorta from Oregon and Pinus flexilis growing in Idaho, have been obtained by hydrodistillation and analyzed by gas chromatographic techniques. The leaf essential oil of P. ponderosa was dominated by β-pinene (21.5–55.3%), methyl chavicol (8.5–41.5%), α-pinene (3.6–9.6%), δ-3-carene (3.6–6.2%), and α-terpineol (1.4–5.3%). The major components of P. contorta essential oil were β-phellandrene (23.8%), terpinen-4-ol (11.0%). The essential oil of P. flexilis was dominated by α-pinene (37.1%), β-pinene (21.9%), bornyl acetate (12.8%), and camphene (8.5%). Chiral gas chromatography revealed the enantiomeric ratios of α-pinene and limonene to be variable, but (−)-β-pinene predominated in Pinus essential oils.

Field larvicidal and oviposition repellent efficacy of three essential oil formulations for the control of filaria vector mosquito Culex quinquefasciatus Say (Diptera: Culicidae): A kill and push concept

The field larvicidal and oviposition repellent efficacies of emulsifiable formulations of botanical oils, (CN-citronella oil, EU-eucalyptus oil, PN-pine oil, PN+EC-combination of pine and eucalyptus oil in 1:1 ratio), against Culex quinquefasciatus mosquitoes. • Larval mosquito control is vital to curb vector population and thereby disease transmission. • Synthetic larvicides cause insecticide resistance and environmental toxicity in mosquitoes. • Three botanical oils were tested against filaria vector Culex quinquefasciatus mosquitoes. • Eucalyptus and pine oils were potent larvicide and oviposition repellent than citronella. • The emulsifiable formulations are effective for multiple weeks under the field conditions. Biological control of larval mosquitoes is in great demand due to the development of resistance against synthetic insecticides, environmental toxicity and the inability to protect habitats from further oviposition. In the present study, three botanical essential oils (BEOs) – citronella, eucalyptus, and pine oils – were formulated for the assessment of larvicidal and oviposition repellent efficacies against Culex quinquefasciatus Say , the filaria vector . The GC–MS profiling of BEOs showed the presence of 16 – 19 compounds covering 87.7–93% of oil composition. The resistance status of Culex quinquefasciatus population was evaluated with temephos (LC 50 = 0.001 ppm, LC 90 = 0.01 ppm) . Larval bioassay of emulsifiable concentrate (EC) formulations prepared from eucalyptus and pine oils showed promising efficacy (LC 50 = 22.7 and 23.2 ppm) and LC 90 (63.8 and 62.4 ppm) compared to citronella oil EC (LC 50 = 43.4 ppm and LC 90 = 199.0 ppm). The field trials of eucalyptus + pine (1:1 ratio) EC showed 100% larval mortality for 3 weeks at 300 ppm compared to 2 weeks of individual oils. Further, the oviposition attraction index (OAI) for ECs of eucalyptus, pine, and their combination showed complete protection of breeding habitats from oviposition at 1st week and −0.9 to −1.0 OAI at 2nd week with slight reduction to −0.5 at 3rd week. Citronella EC provided shortest larvicidal and oviposition repellent efficacy under the field conditions. The promising mosquitocidal activities of EC formulations of eucalyptus and pine or their combination suggest them as potential biocontrol vector control candidates over citronella oil.

Anthracnose Disease Control and Postharvest Quality of Hass Avocado Stored in Biobased PLA/PBAT/Pine Essential Oil/Chitosan Active Packaging Nets.

For sale in the domestic market, Hass avocados are kept in non-biodegradable plastic nets and stored at an ambient temperature. The fungus Colletotrichum gloeosporioides can cause important losses at this stage. Consequently, formulations based on polylactic acid (PLA)/poly (butylene adipate-co-terephthalate) (PBAT) 60/40 biodegradable blends added with pine essential oil (PEO) at 10, 12, 14, and 20% and coated with 1% chitosan (CH) were used for the elaboration of nets, which were evaluated on C. gloeosporioides and Rhizopus stolonifer on nutrient media and fruit and on the ripening behavior of Hass avocados at ambient temperature. The spore germination stage of C. gloeosporioides was the most extensively damaged (78% inhibition). The incidence of anthracnose was notably reduced by almost 80% in the avocados stored in the 60/40 PLA/PBAT nets coated with 1% CH. The overall values regarding weight loss, °Brix, and dry matter were c.a. 23.5%, 7.5, and 24.5%, respectively. The changes in firmness, color, and CO2 production were particularly associated with the initial harvest index and storage temperature rather than with the nets. In future research, essays on nets should include evaluations at the commercial levels.

The effect of pine trees surrounding wheat fields on the infestation of wheat sunn pest, Eurygaster integriceps in Shahr-e-Rey/Tehran region

The sunn pest, Eurygaster integriceps Put. (Hem.: Scutelleridae) is one of the most important pests of wheat in Iran and the Middle East. At present, the control of this pest in the country is based on chemical control, which due to the limitation of using chemical pesticides, the use of other eco-friendly control methods, including the cultivation of companion plants is considered. Therefore, in this study, the effect of windbreakers, especially pine trees, around wheat fields, on the infestation of the sunn pest was investigated during 2019 and 2020. In this experiment, three treatments were considered in three replications, including three areas surrounded by pine trees, three areas surrounded by non-pine trees, and three areas without windbreakers. In each area, sampling of the mother's bug was performed several times by quadrate and insect net during an outbreak of pest in two consecutive years. Furthermore, the correlation of meteorological parameters with the pest density at sampling dates in different treatments was investigated. As well as, in the laboratory studies, the repellency of pine essential oils on the adult insect was tested in three concentrations, including LC10, LC25, LC50 by olfactometer and wind tunnel. The results of field sampling showed that the density of the pest in fields with windbreakers, especially pine windbreakers, was much lower than the farms without windbreakers in both years. Bioassay results showed that pine trees essential oils have a high lethal effect on adult bug, and the values of LC50, LC25 and LC10 were 362, 82 and 21 µl/l of air, respectively. The results also showed that increasing the concentration of essential oils increased the rate of repellency. The results of the correlation of meteorological parameters with adult bug density showed that in all treatments, there was a positive and significant correlation between sunn pest density and wind direction in both studied years. Therefore, based on this study's results, planting of pine trees around wheat fields can reduce infestation of sunn pest, and the repellency properties of the volatile compounds of this plant on the pest are probably adequate.

Effect of hydrochloric polyhexamethylene guanidine (PHMGH) and polyhexamethylene biguanidine (PHMBH), also in combination with plant essential oils and ZnO nanoparticles on some eukaryotic cattle and pig cells

Aim. To investigate both toxic (hemolytic), and stimulating effects of two polymeric derivatives of guani- dine, in particular, polyhexamethylene guanidine (PHMG) and polyhexamethylene biguanidine (PHMB) both in the hydrochloride form, on eukaryotic cells depending on the concentration of the preparation; to study the possibility of using wound-healing and stimulating properties of these preparations in veterinary medicine. Methods. The hemolytic activity (toxicity) of PHMGH and PHMBH preparations in the concentration of 0.1% towards cattle and pig erythrocytes was determined by titration. Primary cell cultures of fetal kidney cells of calves and piglets were used to determine the influence of PHMGH and PHMBH both alone and in combination with the following biologically active substances: essential oils of Pinus sylvestris, Eucalyptus globulus, Citrus sinensis, Monarda didyma, ZnO nanoparticles (size c. 25 nm), and electrochemically acti- vated water – anolyte (Eh = –800 mV, pH 6.5–7.0). The concentration of the cells in the nutrient medium was determined via photocolorimetry. Results. It was found that depending on the concentration, PHMGH, and PHMBH preparations can cause the lysis of erythrocytes, and stimulate cell proliferative activity, including the formation of a monolayer of kidney cells of calves and piglets They cause hemolysis of cattle erythro- cytes in the concentrations commonly used for disinfection, i.e., about 0.1 %, in the average titers of 1 : 7 for PHMGH and 1 : 2.5 for PHMBH. Therefore, PHMBH shows greater hemolytic (biocidal) activity for cattle erythrocytes than PHMGH (in ≈2.8x). The high molecular weight fraction of PHMBH (M2 ≈ 2,000–7,000 Da) demonstrated a lower (in ≈2.4x) hemolytic activity than the low molecular weight basic fraction (M1 ≈ 500–2,000 Da). The experiments on the kidney cell cultures of pigs and cattle have shown that at non-toxic concentrations (10–5 %) PHMBH can effectively stimulate (from 27 to 65 % increase) the proliferative activ- ity of eukaryotic cells and accelerate the formation of a monolayer of cells. The combinations of PHMGH with some essential oils of medicinal plants also show a good effect (from 52 to 95 % increase), and PHMBH shows a good effect with oil of pine for pig kidney cells (20 % increase) and oil of horsemint for cattle kidney cells (67 % increase). Conclusions. PHMGH and PHMBH can possibly be used in agricultural production not only as disinfectants or antiseptics, but also in wound healing. Although their toxicity is also significant to eukaryotic cells, yet they can possibly be used in veterinary medicine in low concentrations (0.005–0.5 %) for the treatment of wounds of various origin, including burns, in the composition of ointments, gels, bandages, or plasters, which we have presently in investigation.

Modification of Natural Carbolic Acid from Arpus wit Lemongrass Essential oil as a Disinfectant

Carbolic acid is a cleaning fluid or disinfectant that can be used to clean various kinds of surfaces, especially floors. Carbolic acid is used to prevent germs and viruses from growing so as to prevent disease and its spread. Most housewives often use chemical-based floor cleaners that are immediate or more practical. Long-term and too frequent use can be harmful to the body and the environment. Therefore, one solution to minimize this is to use natural ingredients that are already available and developed in Lubuklinggau City, such as lemongrass to be used as carbolic acid. The preparation begins by dissolving the arpus into the NaOH solution until it dissolves, then adding a mixture of pine oil, teepol and propylene glycol until everything is homogeneously mixed. Carbol is ready to use. The resulting product is carbolic floor disinfectant. Modified natural carbolic acid from arpus and lemongrass can be used as a disinfectant because based on the results of research that has been done it can kill E-coli bacteria and has a fairly high effectiveness with an inhibition zone of 19.2 mm.

Study of gold ore processing by flotation methods

The article deals with the studies of gold-bearing ore benefication by flotation methods. The object of the study is a low-sulfide gold-quartz type of ores with the following petrographic composition: quartz – 90 %, quartz-chlorite schists – 10 %. The ore from this deposit consists of the weathering crust rocks including iron-mica rocks with veins and spots of granoblastic quartz. The purpose of the research is to develop an optimal flotation regime for obtaining sulfide gold-bearing concentrate. The influence of ore size, reagent mode, flotation redistribution structure, and flotation time on operations was recorded during the experiment. The paper presents the results of studying the chemical composition of the ore by the method of silicate and optical emission analysis. The flotation process used butyl potassium xanthate as a collector and a combination of pine essential oils as a foaming agent. The Hancock concentration efficiency criterion was determined based on a series of experiments. The following technological indicators of ore processing were identified: gravity concentrate with the gold grade of 1165 g/t with the yield of 0.3 % and the recovery of 73.74 %; flotation concentrate (after purification II) with the gold grade of 68.9 g/t with the yield of 1.52 % and a recovery of 22.05 %. Its silver content was 15.9 g/t. The total gold recovery was 95.79 %, with the yield of 1.82 % and the gold grade of 249.9 g/t. The gold grade in the flotation tailings was 0.19 g/t.

Pinus sylvestris L. and Syzygium aromaticum (L.) Merr. & L. M. Perry Essential Oils Inhibit Endotoxin-Induced Airway Hyperreactivity despite Aggravated Inflammatory Mechanisms in Mice.

Scots pine (SO) and clove (CO) essential oils (EOs) are commonly used by inhalation, and their main components are shown to reduce inflammatory mediator production. The aim of our research was to investigate the chemical composition of commercially available SO and CO by gas chromatography–mass spectrometry and study their effects on airway functions and inflammation in an acute pneumonitis mouse model. Inflammation was evoked by intratracheal endotoxin and EOs were inhaled three times during the 24 h experimental period. Respiratory function was analyzed by unrestrained whole-body plethysmography, lung inflammation by semiquantitative histopathological scoring, myeloperoxidase (MPO) activity and cytokine measurements. α-Pinene (39.4%) was the main component in SO, and eugenol (88.6%) in CO. Both SO and CO significantly reduced airway hyperresponsiveness, and prevented peak expiratory flow, tidal volume increases and perivascular edema formation. Meanwhile, inflammatory cell infiltration was not remarkably affected. In contrast, MPO activity and several inflammatory cytokines (IL-1β, KC, MCP-1, MIP-2, TNF-α) were aggravated by both EOs. This is the first evidence that SO and CO inhalation improve airway function, but enhance certain inflammatory parameters. These results suggest that these EOs should be used with caution in cases of inflammation-associated respiratory diseases.

Comparison of the effectiveness of selected essential oils with mineral oil and spinosad on Dermanyssus gallinae.

It seems that essential oils can be a good ingredient in effective preparations against Dermanyssus gallinae. Dermanyssus affects animal health leading to financial losses and bird welfare issues. Collected mites were treated with various essential oils in four (20, 50, 80 and 100%) concentrations at a dose of 0.28 mg/cm2. The direct toxicity of the essential oils, mineral oil and spinosad to D. gallinae was tested in the laboratory. Eucalyptus oil was the most toxic essential oil in all concentrations to D. gallinae (87.6 - 97.6% mortality at all four concentrations), while geranium, pine and rosemary oils showed mortality rates of 14.2 - 68.2%. High mortality after 48 hours of contact was also recorded for the oil of cloves at 80% dilution (85.1% mortality), lavender 100% (94.2% mortality). Similarly, the thyme essential oil produced 83.5 - 93.2% mortality in three concentrations: 50, 80 and 100%. The mineral oil was the least effective oil against mites. Spinosad showed high effectiveness against D. gallinae.

Effect of pine essential oil and rotating magnetic field on antimicrobial performance

This work presents the results ofa study which concerns the influence of rotating magnetic field (RMF) on the antibacterial performance of commercial pine essential oil. A suspension of essential oil in saline solution and Escherichia coli were exposed to the rotating magnetic Afield (the frequency of electrical current supplied by a RMF generator f = 1–50 Hz; the averaged values of magnetic induction in the cross-section of the RMF generator B = 13.13 to − 19.92 mT, time of exposure t = 160 min, temperature of incubation 37 °C). The chemical composition of pine (Pinus sylvestris L.) essential oil was determined by gas chromatography coupled with mass spectrometry (GC–MS). The main constituents were α-pinene (28.58%), β-pinene (17.79%), δ-3-carene (14.17%) and limonene (11.58%). The present study indicates the exposition to the RMF, as compared to the unexposed controls causing an increase in the efficacy of antibacterial properties of pine oil. We have shown that rotating magnetic fields (RMF) at a frequency, f, between 25 Hz to and 50 Hz increased the antimicrobial efficiency of oil a concentration lower than 50%.

Improvement of the reagent regime for the flotation enrichment of polyhalite ores

The effect of foaming agents of different chemical nature (amyl alcohol, glycolic ether, pine oil) on the flotation of halite from polyhalite ores using alkylmorpholine as a collector was investigated. It was established that the combined adding of amyl alcohol with alkylmorpholine and glycolic ether with alkylmorpholine in the process of sodium chloride flotation allows achieving high technological performance and reduces the consumption of expensive alkylmorpholine by 2 times. The use of pine oil as a foaming agent in a mixture with alkylmorpholine has been established to reduce the collector consumption by 2.2 times.