The anchor pile is widely used in marine aquaculture, and its uplift resistance capacity determines the safety performance of the marine aquaculture structure. Cyclic loads such as wind, waves, and currents in the marine environment affect the uplift resistance capacity of anchor piles. By carrying out a cyclic loading model test of anchor piles for marine aquaculture, the influence of loading amplitude, initial tension angle, and other factors on the uplift resistance of anchor piles was investigated. The experimental results showed that with an increase in the loading amplitude, the cumulative displacement and elastic displacement of the anchor pile under vertical and oblique loading increase, and the stiffness of the soil around the anchor piles decreases. The stability of the anchor piles is reduced. When the loading amplitude is the same, with the increase in the initial loading angle, the lateral cumulative displacement of the anchor pile increases. Meanwhile, the vertical cumulative displacement decreases, the stiffness of the soil around the anchor pile decreases, and the stability decreases.