The aim of this study was to evaluate the effects of dietary phospholipids (PL) sources (fish gonad G-PL and soybean lecithin S-PL) and levels (50 and 90 g kg−1 dry matter) on the performances and fatty acid (FA) composition of pikeperch larvae. From day 10 to day 34 posthatching (p.h.), larvae were fed with three isoproteic and isolipidic microdiets. The best results of growth and skeletal development were related to a high phospholipid level regardless of their origin and FA profile. Jaw deformities seemed associated with high dietary highly unsaturated FA (HUFA) level. The optimal level of eicosapentaenoic acid and docosahexaenoic acid (EPA + DHA) for pikeperch larvae appeared to be around 12 g kg−1 (dry matter) associated with a PL level around 90 g kg−1. FA composition of diets and larvae revealed a better incorporation of arachidonic acid, EPA and DHA into PL fraction especially in larvae fed with soybean PL. Moreover, 34-day-old pikeperch larvae may have capability of converting 18 carbon n-3 FA into the n-3 HUFA. Hence, for pikeperch larvae, PL from plant origin were as efficient as those from marine fish origin.