Disease In Pigs Research Articles

The Influence of the Temperature on Effectiveness of Selected Disinfectants Against African Swine Fever Virus (ASFV)

African swine fever (ASF) is one of the most economically significant diseases of pigs caused by African swine fever virus (ASFV). Due to the lack of effective and safe vaccines, one of the crucial measures to protect farms from the introduction of the ASFV is to apply a strict regime of biosecurity and disinfection. However, in field conditions, the activity of disinfectants may be influenced by temperature, resulting in reduced activity or biodegradation (i.e., freezing or evaporating). The aim of this study was to evaluate the effect of a wide range of temperatures on the virucidal activity of selected active substances commonly used against ASFV. Eight active substances were tested, namely: sodium hypochlorite (1.0%), glutaraldehyde (0.1%), potassium peroxysulfate (0.5%), caustic soda (1.0%), phenol (1.0%), acetic acid (3.0%), benzalkonium chloride (1.0%), and formaldehyde (0.4%). The virucidal activity of each compound was tested at different temperatures (21, −10, and −20 °C for 30 min) and compared to the initial virus titer under the same temperature conditions. Exposure to a range of temperatures did not significantly affect the virucidal efficacy of tested active substances against ASFV. Most of the evaluated substances had reduced virus titers ≥ 4 log10, regardless of the temperature. However, two of them (benzalkonium chloride and acetic acid) were sensitive to sub-zero temperatures, showing a lack of the required 4 log10 virus titer reduction. The conducted study showed that temperature could hamper the virucidal effect of selected substances (i.e., benzalkonium chloride and acetic acid), showing their moderate efficacy against ASFV −10 °C and −20 °C. The results suggest that extreme caution should be taken while applying these substances at sub-zero temperatures. The other substances had no significant sensitivity to the temperature range. Nevertheless, in the case of freezing the agent, insufficient penetration of the disinfected surface may occur, which may result in an ineffective disinfection process.

Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes

IntroductionPorcine epidemic diarrhea virus (PEDV) is a pathogen that causes a highly contagious intestinal disease in pigs, which causes significant economic losses to the pig industry worldwide. PCR is the most commonly used technique for PEDV diagnosis in practical clinics, however, reported works still suffer from shortcomings, for example, most of them cannot differentiate GI and GII subtypes, they suffer from low sensitivity, and some primer sequences are no longer able to match the mutant strains.MethodsTo address these issues, we conducted a comprehensive analysis by comparing the sequences of the PEDV S protein in the existing NCBI database with a recently isolated epidemic strain of PEDV, named SX0818-2022, of subtype GIIa from Shanxi, China. The conserved sequences of GI and GII subtypes were retrieved to design the primers and probe. Leveraging this information, we developed a TaqMan probe-based quantitative real-time PCR (qPCR) assay that is uniquely tailored to detect both PEDV GI and GII subtypes.ResultsAdditionally, this qPCR can identify PEDV GI and GII subtypes with high sensitivities of 90 copies/μL and 40 copies/μL, respectively (refers to the number of copies of the DNA target per microliter of template in the reaction system), much higher than the previously reported works and especially suitable for early diagnosis and prevention. Besides, excellent specificity and repeatability of the duplex qPCR were verified, thus supporting its potential applications in practical clinics.DiscussionTherefore, this work presents a promising tool for PEDV diagnosis, prevention, and control.

Genetic profile of the whole genome sequence of African swine fever virus from the first outbreak in Malaysian Borneo.

African swine fever (ASF), a severe and highly contagious haemorrhagic viral disease of pigs, is becoming a major threat not only in Malaysia but around the world. The first confirmed case of ASF in Malaysia was reported in February 2021. Despite the emergence of ASF in Malaysia, genetic information on this causative pathogen for the local livestock is still limited. This study aimed to genetically characterize the African swine fever virus (ASFV) responsible for the 2021 outbreak in Malaysia. The genome of the ASFV isolated during the first outbreak in Malaysia was analysed as ASFV/Sabah/Malaysia/1160/21 which has 190,594 base pairs, with a nitrogenous bases (GC) content of 40.33% and 195 predicted Open Reading Frames (ORF). The complete genome sequence was compared with other annotated ASFV genomes retrieved from database of National Center for Biotechnology Information (NCBI) to obtain information based on target gene B646L, E183L, intergenic region (IGR) between I73R and I329L (IGRI73R-I329L), EP402R and B602L. The ASFV/Sabah/Malaysia/1160/21 genome had a high similarity percentage to the reference genome, Georgia 2007 and all Southeast Asian strains. Phylogenetic analysis revealed that the ASFV strain belonged to genotype II, serogroup 8, CVR1 and showed high characteristics of IGR variant II based on IGRI73R-I329L. This study expands our understanding of genetic diversity and provides significant insights into the genomic characteristics and variation of ASFV strains that are circulating in Malaysia.

A PDMS/chitosan/MPMs composite film based on multi-field coupling enhancement for African swine fever virus P72 protein detection.

African swine fever (ASF) is an acute hemorrhagic disease in pigs caused by the African swine fever virus (ASFV), which has a high mortality rate and brought great damage to global pig farming industry. At present, there is no effective treatment or vaccine to combat ASFV infection, so early detection of ASFV has become particularly important. Therefore, the PDMS/chitosan/MPMs composite film was proposed to detect ASFV P72. PDMS mixed with chitosan particles were used to provide biological modification sites for the ASFV P72 antibody, which simplified the functional modification process. The specific binding of antigen and antibody leads to the mechanical deformation of the composite film, which was observedthrough optical method tochange the microsphere spacing. The polystyrene microsphere (PMs) spacing changes were acquired by simply measuring the diameters of the Debye ring diffracted by the PDMS/chitosan/PMs with a simple laser pointer. The conductive layer formed by AgNWs realize the transformation of biological signals to electrical signals. By conjugating PMs with Fe3O4 nanoparticles (MPMs) and applying a magnetic field, a magnetic-stress-electric coupling system has been constructed, which amplifies the deformation of the composite film and enhances the sensitivity of the flexible film. The experimental results showed that the flexible film has wide linear range of 10ng/mL-100μg/mL, and the optimal detection limit (LOD) is 0.059ng/mL, which was aboutone order of magnitude lower than that without magnetic field. In addition, the flexible film showed a good stability, specificity, and selectivity.

Effective and sustainable prevention and control of pork tapeworm diseases in humans and pigs in Tanzania: a policy brief

Effective and sustainable prevention and control of pork tapeworm diseases in humans and pigs in Tanzania: a policy brief

Detection of antimicrobial resistance in Glaesserella parasuis in South China using whole-genome sequencing.

Glaesserella parasuis causes Glässer's disease in pigs, a leading cause of death in swine herds and a major contributor to economic losses in the global swine industry. Although several studies have investigated antimicrobial resistance in G. parasuis, the correlation between phenotypic and genotypic resistance remains unclear due to incomplete genetic resistance mechanisms detection. The susceptibility of 117 clinical G. parasuis isolates to 7 antimicrobials was determined using a broth microdilution method. The sequences of 48 resistant isolates were obtained by whole-genome sequencing. Resistance genes, mutations, and group 1 vtaAs were detected based on whole-genome sequence data. Sequence types (STs) were identified by multilocus sequence typing (MLST). Phenotypic analysis showed that most isolates were susceptible to the tested antibiotics; resistance was most common against tetracycline (27%) and enrofloxacin (20%). All isolates were susceptible to ceftiofur. Analysis of whole-genome sequences revealed that resistance to tetracycline, amoxicillin, erythromycin, florfenicol, and chloramphenicol was frequently associated with the resistance genes tet(B) or tet(H), bla ROB-1 , erm(T), floR, and catA3, and enrofloxacin resistance was associated with mutations in GyrA, ParC, and ParE. MLST identified 25 STs, of which, 14 were novel. The sequenced strains were divided into two primary lineages, LI and LII. Group 1 vtaA genes were detected in 87.5% (n = 42) of the isolates. This study provides comprehensive insights into the molecular mechanisms responsible for drug resistance in G. parasuis, the characteristics of molecular epidemiology, and the virulence of resistant groups. Our findings can aid in the development of G. parasuis-specific clinical breakpoints and inform strategies for managing antimicrobial resistance in swine herds.

Construction of a recombinant African swine fever virus with firefly luciferase and eGFP reporter genes and its application in high-throughput antiviral drug screening.

African Swine Fever (ASF) is a highly lethal and contagious disease in pigs caused by African Swine Fever Virus (ASFV), which primarily infects domestic pigs and wild boars, with a mortality rate of up to 100%. Currently, there are no commercially available vaccines or drugs that are both safe and effective against ASFV. The ASFV 0428C strain was continuously passaged in Vero cells, and the adapted ASFV demonstrated efficient replication in Vero cells. The adapted ASFV was used as the parental virus, and an expression cassette encoding a dual reporter gene for firefly luciferase (Fluc) and enhanced green fluorescent protein (eGFP) was inserted into the ASFV genome using CRISPR/Cas9 gene editing technology to construct a recombinant ASFV variant (rASFV-FLuc-eGFP). rASFV-Fluc-eGFP was genetically stable, effectively infected porcine alveolar macrophages (PAM) and Vero cells, and expressed Fluc and eGFP concurrently. This study provides a tool for investigating the infection and pathogenic mechanisms of ASFV, as well as for screening essential host genes and antiviral drugs. Additionally, a high-throughput screening model of antiviral drugs was established based on rASFV-FLuc-eGFP in passaged cells, 218 compounds from the FDA-approved compound library were screened, and 5 candidate compounds with significant inhibitory effects in Vero cells were identified. The inhibitory effects on ASFV were further validated in both Vero and PAM cells, resulting in the identification of Salvianolic acid C (SAC), which demonstrated inhibitory effects and safety in both cell types. SAC is a candidate drug for the prevention and control of ASFV and shows promising application prospects.

Tomographic and ultrasound evaluation of the thyroid gland in pet guinea pigs.

There has been increasing interest in the literature on primary thyroid diseases in guinea pigs, which are believed to be underdiagnosed. This study describes the size and characteristics of the thyroid gland on ultrasound (US) and CT examinations in the guinea pig (Cavia porcellus) without evidence of thyroid pathology and examines the possible use of US as a routine examination for thyroid diseases. This prospective and anatomical study included 20 guinea pigs that underwent CT for dental pathologies and US for thyroid gland (TG) examination. Based on US and CT images, this study evaluated the thyroid lobe dimensions (length, height, and width) and shape. Echogenicity and homogeneity were assessed on US images, and attenuation values (HU) and homogeneity were evaluated on CT images. All animals underwent CT and US examinations under anesthesia. Thyroid lobes appeared more frequently as fusiform on CT (65%) and oval on US scans (67.5%). The attenuation values on CT scans were homogeneous. On the US scans, the TG was more frequently hypoechoic. No statistically significant differences were found between the size of the right and left thyroid lobes, both with CT and US. A statistically significant difference was found between the length values on CT and US. The US and CT thyroid gland dimensions, shape, and appearance were described. Both CT and US are useful techniques for evaluating the TG in guinea pigs.

Selected topics of the 27th IPVS Congress in Leipzig. The impact of changes in swine production and globalization of pig trade on the appearence and spread of transboundary diseases.

Between June 4 and 7 2024, the 27th Congress of the International Pig Veterinary Society (IPVS) took place in Leipzig, Germany. The meeting took place in a modern congress center located on the outskirts of this historic Saxon city with a history of more than a thousand years. Polish practitioners and scientists involved in the field of swine diseases have been participating in IPVS congresses since the beginning. Our delegation is usually one of the most numerous. In Leipzig, the IPVS congress was held for the first time simultaneously with the 15th European Symposium of Porcine Health Management (ESPHM), which has been organized for many years by the European College of Pig Health Management (ECPHM). Hence, IPVS&ESPHM affiliations appear in all materials related to the congress. During the congress, 1,035 reports were presented in oral or poster form. Plenary lectures were of particular interest. The 17 lectures were presented by internationally recognized scientists and practitioners invited by the organizers. Analyzing the subject matter of the papers submitted to the congress, it can be concluded that the most important topics over the last two years have been viral diseases of pigs, including circovirus infections, PRRS, and ASF. Scientists predict that, due to climate change, at least 15,000 viruses will move between different mammalian species in the next 50 years. Human mortality from zoonoses is projected to increase 12-fold between now and 2050.

From structure prediction to function: defining the domain on the African swine fever virus CD2v protein required for binding to erythrocytes.

A better understanding of the interactions between viruses and their hosts is a crucial step in the development of strategies for controlling viral diseases, such as vaccines and antivirals. African swine fever, a pig disease with fatality rates approaching 100%, causes very substantial economic losses in affected countries, and new control measures are clearly needed. In this study, we characterized the interaction between the ASFV CD2v protein and host erythrocytes. The interaction plays a key role in viral persistence in blood since it can allow the virus to "hide" from the host immune system. We identified the amino acids in the viral protein that mediate the interaction with erythrocytes and used this information to construct a mutant virus that is no longer able to bind these cells. This virus induces strong immune responses that provide high levels of protection against infection with the deadly parental virus.

Molecular detection and evolutionary analysis of porcine epidemic diarrhea virus in Henan and Shaanxi provinces in China.

Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious and devastating enteric disease of pigs characterized by diarrhea, dehydration and 80-100% mortality in piglets, leading to substantial economic losses in the global swine industry. To investigate the prevalence of PEDV in Henan and Shaanxi provinces of China from 2022 to 2023, a total of 87 clinical samples (including intestinal tissues and faeces) were collected from diseased piglets during outbreaks of diarrhea on pig farms. Of the 87 samples, 32 (36.7%) tested positive for PEDV by RT-PCR, and the complete S gene from nine positive samples and the ORF3 gene from 11 positive samples, all from different farms, were subsequently sequenced. Phylogenetic analysis based on S gene sequences showed that most of the nine PEDV isolates sequenced belonged to the G2a clade and were most closely related to variant strains of PEDV. However, two strains from Shaanxi (CH-SX1-2023 and CH-SX2-2023) belonged to the G2b subtype, and one strain from Henan (CH-HNC2-2023) belonged to the S-INDEL branch. Amino acid sequence comparisons showed that there were several amino acid substitutions and deletions in the S and ORF3 proteins of the PEDV strains from Henan and Shaanxi compared to the CV777 vaccine strain. Some of these mutations occurred in neutralizing epitopes, particularly COE and might therefore be associated with the ineffective protection provided by existing vaccines. These results will provide insights for better understanding of the epidemiological situation of PEDV in Henan and Shaanxi provinces between 2022 and 2023, and this information also contributes to the development of new strategies for the prevention and control of variant PEDV strains.

Assessment of the Effect of Deleting the African Swine Fever Virus Gene R298L on Virus Replication and Virulence of the Georgia2010 Isolate.

African swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia. The causative agent, ASF virus (ASFV), is a large, double-stranded and structurally complex virus. The ASFV genome encodes for more than 160 proteins; however, the functions of most of these proteins are still in the process of being characterized. The ASF gene R298L, which has previously been characterized as able to encode a functional serine protein kinase, is expressed late in the virus infection cycle and may be part of the virus particle. There is no description of the importance of the R298L gene in basic virus functions such as replication or virulence in the natural host. Based on its evolution, it is proposed that there are four different phenotypes of R298L of ASFV in nature, which may have potential implications for R298L functionality. We report here that a recombinant virus lacking the R298L gene in the Georgia 2010 isolate, ASFV-G-∆R298L, does not exhibit significant changes in its replication in primary cultures of swine macrophages. In addition, when experimentally inoculated in pigs, ASFV-G-∆R298L induced a fatal form of the disease similar to that caused by the parental virulent ASFV-G. Therefore, deletion of R298L does not significantly affect virus replication and virulence in domestic pigs of the ASFV Georgia 2010 isolate.

Oreochromicin-2 shows antimicrobial and immunostimulant effect against respiratory pathogens in pigs.

Porcine respiratory diseases have a huge economic impact on pig production. The highest incidence of these diseases is commonly linked to Streptococcus suis, Pasteurella multocida and Bordetella bronchiseptica, some of which are zoonotic posing a risk to human health. The inappropriate and excessive use of conventional antibiotics, as usual procedure for treating respiratory diseases in pigs, has generated the emergence of antimicrobial resistance (AMR), which urgently requires the development of alternative approaches to current antimicrobials. Antimicrobial peptides (AMPs) have rapidly garnered interest as novel therapeutic candidates. Oreochromicin-2 (Oreoch-2), an AMP previously isolated from Oreochromis niloticus gills, has shown broad antibacterial properties against several species. However, studies about its effect on porcine respiratory pathogens and its potential use for the treatment of swine respiratory diseases are not available. In this work we determined the in vitro antimicrobial activity of the peptide against S. suis by a broth microdilution method. Oreoch-2 showed a MIC of 3.13μM against this pathogen. For in vivo experiments, Yorkshire x Landrace crossbred (LYxL35) weaning pigs aged 30-33days were allocated in pens containing ten pigs each. To study the influence of the peptide on health status, a total clinical score was determined. The administration of Oreoch-2 improved the clinical behavior of the animals, similar to the conventional antibiotic shotapen, respect to the placebo group. A reduction of bacterial loads in the respiratory tract and lungs was observed in Oreoch-2-treated animals as compared to the placebo group. It was shown that peptide treated-piglets displayed significantly higher serum IgG concentration compared to the control group. These results demonstrated Oreoch-2 potential as an antimicrobial and immunostimulant drug candidate against respiratory diseases in pigs.

Berbamine inhibits Pseudorabies virus in vitro and in vivo.

Pseudorabies virus (PRV) is a significant pathogen that causes acute infectious diseases in pigs, resulting in considerable economic losses for the global pig industry. The lack of effective control measures and vaccines against the circulating variants of PRV highlights the pressing need for novel treatment strategies. In this study, a screening of a natural product library identified Berbamine as a promising compound that inhibits PRV replication, with a selectivity index of 17. Preliminary investigations demonstrated that Berbamine impedes viral proliferation by targeting the replication and release stages of the PRV life cycle. In experiments with mice artificially infected with PRV, Berbamine significantly alleviated clinical symptoms and histopathological changes in brain tissue caused by PRV infection. Furthermore, molecular docking studies indicated that Berbamine targets the UL50 protein, not only of PRV but also of HSV-1, FHV-1, and BoHV-1. Given that the UL50 protein is a promising target for antiviral drug development, Berbamine holds considerable potential for broad application in antiviral therapies.

Study on the Effect of Phillyrin on Streptococcus suis In Vivo and In Vitro.

As a zoonotic pathogen, S. suis serotype 2 (SS2) can cause severe diseases in both pigs and humans, and develop resistance to antibiotics. Plant natural compounds are regarded as promising alternatives to conventional antibiotics. Phillyrin is the major bioactive components of Chinese herbal medicine Forsythia suspensa. In this study, we explored the activity and action mechanism of phillyrin against SS2. The results showed that phillyrin could disrupt membrane integrity, destroy intracellular structures, and increase the exosmosis of DNA. Results of PCR revealed that phillyrin affected bacterial-virulence-related genes' expression levels. Meanwhile, phillyrin significantly decreased the adhesion activity, inhibited lactate dehydrogenase (LDH) secretion, and reduced biofilm formation of SS2 in Newborn pig trachea epithelial (NPTr) cells. Furthermore, phillyrin protected tight junction protein of NPTr cells from SS2. We reported that phillyrin (0.1 mg/kg) treatment after bacterial challenge significantly improved the survival rate, ameliorated pulmonary inflammation, and inhibited the accumulation of multiple cytokines (IL-1, IL-6, IL-8, and TNF-α). Molecular docking showed that phillyrin had a good binding activity with the Ala88 and Asp111 of suilysin (SLY), one of the most important virulence factors of SS2. Collectively, phillyrin possesses antibacterial and anti-inflammatory activities, and is a promising candidate for preventing SS2 infection.

A New Perspective on Pig Genetics and Breeding: microRNA.

microRNA (miRNA) is a class of small non-coding RNA molecules that are widely expressed in organisms and play an important role in the regulation of gene expression at the post-transcriptional level. In recent years, researchers have begun to explore its effects on the development of domestic animals and have begun to think about its potential role in modern molecular breeding. Increasing evidence shows that miRNA play a central role in the regulation of pig fertility, pork product quality and disease resistance. Understanding the physiological mechanism of miRNA will be able to better guide future breeding work. In this paper, we will review the research progress of the function and mechanism of miRNA in combination with the above economic characteristics of pigs. The reported miRNA and their target genes were sorted out to evaluate their potential role in improving economic traits such as pig fertility, meat quality and disease resistance, to provide a reference for modern pig molecular breeding.

Development and application of a quadruplex TaqMan real-time fluorescence quantitative PCR assay for four porcine digestive pathogens.

Salmonella, Escherichia coli, Lawsonella intracellularis, and Brachyspira hyodysenteriae are the primary pathogens responsible for gastrointestinal diseases in pigs, posing a significant threat to the health and productivity of pig production systems. Pathogen detection is a crucial tool for monitoring and managing these infections. We designed primers and probes targeting the invA gene of Salmonella, the 23S rRNA gene of Escherichia coli, the aspA gene of Lawsonella intracellularis, and the nox gene of Brachyspira hyodysenteriae. We developed a quadruplex TaqMan real-time quantitative PCR assay capable of simultaneously detecting these four pathogens. This assay demonstrated high sensitivity, with detection limits of 100 copies/μL for the recombinant plasmid standards pEASY-23S rRNA, pEASY-aspA, and pEASY-nox, and 10 copies/μL for pEASY-invA. The standard curves exhibited excellent linearity (R2 values of 0.999, 0.999, 1, and 0.998, respectively) and high amplification efficiencies (93.57%, 94.84%, 85.15%, and 81.81%, respectively). The assay showed high specificity, with no cross-reactivity detected against nucleic acids from Streptococcus suis, porcine epidemic diarrhoea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), Pasteurella multocida, Clostridium perfringens, Gracilaria parapsilosis, porcine delta coronavirus (PDCoV), porcine group A rotavirus (GARV), and porcine teschovirus (PTV). The assay also exhibited excellent repeatability, with inter- and intra-assay coefficient of variation (CV) ranging from 0.15% to 1.12%. High concentrations of nucleic acids did not interfere with the detection of low concentrations, ensuring robust performance in complex samples. Among 263 diarrhoeic samples, the assay detected Salmonella in 23.95%, Escherichia coli in 26.24%, Lawsonella intracellularis in 33.84%, and Brachyspira hyodysenteriae in 22.43%. This quadruplex TaqMan qPCR assay offers a rapid, sensitive, and specific tool for the simultaneous detection of Salmonella, Escherichia coli, Lawsonella intracellularis, and Brachyspira hyodysenteriae in pigs.

African Swine Fever and Hog Cholera Attacks: Perception and Local Knowledge of Farmers in Manokwari

African Swine Fever (ASF) and Hog Cholera are highly contagious viral diseases in pigs, resulting in losses for pig farmers in Manokwari regency. Farmers cannot avoid panic and resignation. The study aimed to measure pig farmers' attitudes and knowledge about these infectious diseases. Farmers were selected from 12 villages and originated from 8 big ethnics from Papuan and non-Papuan. The findings show that most breeders are still in the early stages of their farming careers and have a long experience. Farmers tend to show concern about African Swine Fever and Hog Cholera. However, their attitudes towards pig diseases are considered less than optimal. Most farmers have fairly good knowledge about African Swine Fever and Hog Cholera. The primary source of information for farmers is obtained from other communities, indicating an exchange of information between pig farmers and the community.

Development and characterization of high-efficiency cell-adapted live attenuated vaccine candidate against African swine fever

ABSTRACT African swine fever (ASF), a contagious and lethal haemorrhagic disease of domestic pigs and wild boars, poses a significant threat to the global pig industry. Although experimental vaccine candidates derived from naturally attenuated, genetically engineered, or cell culture-adapted ASF virus have been tested, no commercial vaccine is accepted globally. We developed a safe and effective cell-adapted live attenuated vaccine candidate (ASFV-MEC-01) by serial passage of a field isolate in CA-CAS-01-A cells. ASFV-MEC-01, isolated via repeated plaque purification using next-generation sequencing analysis, was obtained at passage 18 and showed significant attenuation in 4- and 6-week-old pigs. ASFV-MEC-01 conferred 100% protection against challenge with lethal parental ASFV, which correlated with high ASFV-specific humoral and cellular immune responses. Additionally, ASFV-MEC-01 was not detected in blood until 28 days post-inoculation. Global transcriptome analysis showed that ASFV-MEC-01 lacking 12 genes triggered stronger innate antiviral responses than the parental virus, as exemplified by high levels of mRNA encoding interferon regulatory and inflammatory genes in PAM cells. Ectopic expression of most deleted genes increased replication of DNA viruses by suppressing production of interferons and pro-inflammatory cytokines. Among the genes deleted from ASFV-MEC-01, MGF100-1R interacted specifically with the scaffold dimerization domain of TBK1, thereby preventing TBK1 dimerization and impairing TBK1-mediated type I IFN and NF-κB signalling. These results suggest that attenuation of ASFV-MEC-01 may be mediated by induction of stronger type I IFN and NF-κB signalling within the host innate immune system. Thus, ASFV-MEC-01 could be a safe and effective live attenuated ASFV vaccine candidate with commercial potential.

RGB-based Machine Vision for Enhanced Pig Disease Symptoms Monitoring and Health Management: A Review

RGB-based Machine Vision for Enhanced Pig Disease Symptoms Monitoring and Health Management: A Review