This pilot study evaluated and compared histomorphometric and immunohistochemical characteristics of peri-implant bone tissue after implant site preparation using piezoelectric tips versus conventional drills. Six patients with bilateral partial edentulism underwent a splitmouth protocol. Twelve alveolar ridges were randomized into six control implant sites prepared using conventional drills (Drill Group); and six test implant sites prepared using piezoelectric implant inserts (Piezo Group). At 28 days after surgery (T1), single-stage "study fixtures" with 0.5 mm of peri-implantbone tissue were explanted and processed for histological, histomorphometric and immunohistochemical analysis in both groups. For each sample inflammatory infiltrates, necrotic bone (Zone 1), woven and newly formed bone (Zone 2), native bone (Zone 3), a vascular endothelium differentiation and neo-osteogenesis marker (CD31) and an osteoblastic cell differentiation and osteogenesis marker (SATB2) were evaluated. According to their histological bone features, the three histologically distinct areas were evident in both groups: Zone 1, Zone 2 and Zone 3 according to their histological bone features. Zone 1 showed lower extension in the Piezo Group than in the Drill Group (p=0.028). Regarding the immunohistochemical markers, in all areas of the Piezo Group, SATB2 and CD31 were statistically higher than in the Drill Group. Implant site preparation using piezo surgery results in less bone necrosis, greater osteoblastic activity and greater vessel proliferation compared to the conventional surgical approach.
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