Piece Of Puzzle Research Articles

Universities at the Heart of attractive territories: A Novum Trivium formula in the era of energy consumption optimization

Today, it has become fruitless, reducing the role of the university to its sole mission of education. Undoubtedly, a university is a source of knowledge that acts as an incubator for creativity and innovation; however, more light should be shed on how universities help create a vibrant economy by providing employment opportunities, promoting highquality research, and stimulating investment. In this work, we focus on displaying a new adaptable trinity formula that places the university, with all its missions, in the heart of an attractive territory. It is in this vein that we study the importance of well-choosing the remaining territorial components to be part of the trivium formula. The main aim of the current premise is to highlight the significance of adjusting territorial innovation models, such as business ecosystems of innovation and triple helix models of innovation, in accordance with the weight of keystone components of territory and how they participate in its attractiveness, all of which are orchestrated by universities. The importance of this topic lies in recognizing the potential of universities as accelerators for territorial advancement, including their role in achieving the Sustainable Development Goals, by acknowledging their many distinct contributions to fostering innovation and protecting the environment. Adopting the trivium formula means providing a blueprint that encourages fortifying links within a territory’s components, thus enhancing relations between universities and other pieces of the territorial development puzzle we built this work upon as a method to understand innovative territories, from green innovation to industrial intelligence.

Enhance Safety in Aneurysm Surgery: Strategies for Prevention of Intraoperative Vascular Complications.

Complications during surgery for intracranial aneurysms can be devastating. Notorious pitfalls include premature rupture, parent vessel occlusion, local cerebral injury and brain contusion, and incomplete neck obliteration. These unfavorable intraoperative events can result in major neurological deficits with permanent morbidity and even mortality. Herein, the author highlights the relevant surgical strategies used in his daily practice of aneurysm surgery (e.g., aneurysm clipping with adenosine-induced temporary cardiac arrest), application of which may help prevent vascular complications and enhance surgical safety through reduction of the associated risks, thus allowing improvement of postoperative outcomes. Overall, all described methods and techniques should be considered as small pieces in the complex puzzle of prevention of vascular complications during aneurysm surgery.

The relationship of postocclusive reactive hyperemia assessed by the plethysmographic perfusion index to lactate clearance: a new piece in the unsolved puzzle of tissue perfusion and oxygenation in septic shock

The relationship of postocclusive reactive hyperemia assessed by the plethysmographic perfusion index to lactate clearance: a new piece in the unsolved puzzle of tissue perfusion and oxygenation in septic shock

Microcystin Shows Thresholds and Hierarchical Structure With Physicochemical Properties at Lake Fayetteville, Arkansas, May Through September 2020

Highlights Despite little to no dissolved nutrient supply in surface water, harmful algal blooms are sustained throughout the 2020 growing season. Sediment phosphorus release was high in a lake that has annual harmful algal blooms, and it is an important piece of the watershed management puzzle. Thresholds and hierarchical structure with individual physicochemical properties and pigment fluorescence at this lake explain a large portion of microcystin variability. Abstract. Harmful algal blooms (HABs) in freshwaters are a global concern, and research has focused on the nutrient drivers of cyanobacterial growth and toxin production. We explored the importance of nutrients on sustained cyanobacterial HABs producing measurable microcystin at Lake Fayetteville, Arkansas, USA. The specific objectives were to (1) quantify sediment phosphorus (P) flux and estimate potential equilibrium P concentrations (EPC0) in July 2020, (2) assess water quality conditions in the lake from March through September 2020, and (3) evaluate physicochemical thresholds (or change points, CPs) and hierarchical structure with total microcystin concentrations. The sediments were a potential P source under both oxic and anoxic conditions, and the SRP concentrations in the lake water were continuously less than the EPC0 estimated for bottom sediment (~0.03 mg L-1); sediments are likely a potential P source for cyanobacterial HABs at Lake Fayetteville. The physicochemical changes at Lake Fayetteville over the 2020 growing season were typical of small, hypereutrophic reservoirs, with low biomass in winter when nutrient supply was greatest and the greatest cyanobacterial growth and microcystin toxin as nutrient supply diminished into the growing season. Microcystin concentrations were elevated above 1 µg L-1 from mid-June through mid-August 2020, and most physicochemical parameters in this study showed thresholds or change points with microcystin. Hierarchical structure existed with total microcystin concentrations, showing the potential importance of cyanobacterial biomass, N supply, and total P on elevated microcystin. Nutrients and algal pigment raw fluorescence explained 83% of the variation in total microcystin concentrations at Lake Fayetteville during the 2020 growing season. Nutrients (both N and P) from external and internal sources are likely important drivers of these blooms and toxicity at Lake Fayetteville. Keywords: Harmful Algal Blooms, Nutrient Drivers, Sediment Phosphorus Release, Water Quality.

Reaction dynamics as the missing puzzle piece: the origin of selectivity in oxazaborolidinium ion-catalysed reactions.

The selectivity in a group of oxazaborolidinium ion-catalysed reactions between aldehyde and diazo compounds cannot be explained using transition state theory. VRAI-selectivity, developed to predict the outcome of dynamically controlled reactions, can account for both the chemo- and the stereo-selectivity in these reactions, which are controlled by reaction dynamics. Subtle modifications to the substrate or catalyst substituents alter the potential energy surface, leading to changes in predominant reaction pathways and altering the barriers to the major product when reaction dynamics are considered. In addition, this study suggests an explanation for the mysterious inversion of enantioselectivity resulting from the inclusion of an orthoiPrO group in the catalyst.

Dr. KID: Direct Remeshing and K-Set Isometric Decomposition for Scalable Physicalization of Organic Shapes.

Dr. KID is an algorithm that uses isometric decomposition for the physicalization of potato-shaped organic models in a puzzle fashion. The algorithm begins with creating a simple, regular triangular surface mesh of organic shapes, followed by iterative K-means clustering and remeshing. For clustering, we need similarity between triangles (segments) which is defined as a distance function. The distance function maps each triangle's shape to a single point in the virtual 3D space. Thus, the distance between the triangles indicates their degree of dissimilarity. K-means clustering uses this distance and sorts segments into k classes. After this, remeshing is applied to minimize the distance between triangles within the same cluster by making their shapes identical. Clustering and remeshing are repeated until the distance between triangles in the same cluster reaches an acceptable threshold. We adopt a curvature-aware strategy to determine the surface thickness and finalize puzzle pieces for 3D printing. Identical hinges and holes are created for assembling the puzzle components. For smoother outcomes, we use triangle subdivision along with curvature-aware clustering, generating curved triangular patches for 3D printing. Our algorithm was evaluated using various models, and the 3D-printed results were analyzed. Findings indicate that our algorithm performs reliably on target organic shapes with minimal loss of input geometry.

Severe COVID-19 patients show a dysregulation of the NLRP3 inflammasome in circulating neutrophils.

SARS-CoV-2 triggers inflammasome-dependent release of pro-inflammatory cytokine IL-1β and pyroptosis, therefore contributes to the huge inflammatory response observed in severe COVID-19 patients. Less is known about the engagement of inflammasome in neutrophils, main players in tissue injury and severe infection. We studied the activation of the inflammasome in neutrophils from severe COVID-19 patients and assessed its consequence in term of cells contribution to disease pathogenesis. We demonstrated that NLRP3 inflammasome is dramatically activated in neutrophils from severe COVID-19 patients, and that the specific inhibition of NLRP3 reverts neutrophils' activation. Next, the stimulation of severe patients' neutrophils with common NLRP3 stimuli was not able to further activate the inflammasome, possibly due to exhaustion or increased percentage of circulating immature neutrophils. Collectively, our results demonstrate that the NLRP3 inflammasome is hyperactivated in severe COVID-19 neutrophils and its exhaustion may be responsible for the increased susceptibility to subsequent (and possibly lethal) infections. Our findings thus include a novel piece in the complex puzzle of COVID-19 pathogenesis.

Fifth-Generation District Heating and Cooling Networks Based on Shallow Geothermal Energy: A review and Possible Solutions for Mediterranean Europe

This document presents a comprehensive review of research works, regulatory frameworks, technical solutions, and commercial trends related to the integration of shallow geothermal energy (SGE) technologies in modern 5th-generation district heating and cooling (5GDHC) networks. This literature and market analysis is contextualized by the present geopolitical, environmental, and societal scenario in Europe. In this sense, decarbonization of the heating and cooling sector is a crucial piece in the energy transition puzzle to keep global warming below the critical threshold of 1.5 °C by the next century. Moreover, Ukraine war has added urgency to end up with fossil fuel dependency. The most relevant outcome of this literature review is the synergistic relationship between SGE, 5GDHC networks, and urban environments. SGE is most efficiently deployed in urban environments when it is part of a district heating and cooling network, and the modern concept of 5GDHC is the most suitable scenario for it. Since the potential contribution of SGE to the decarbonization of the heating and cooling supply is mostly untapped across Europe, this synergistic effect represents a possible boost. Hybridization with solar photovoltaics and/or storage makes it even more attractive. Outstanding cases are reviewed, challenges for the future are presented, and tools to overcome social reluctance and/or lack of awareness are described, along with a discussion of the stimuli for the deployment of SGE and 5GDHC networks. A particular focus on Mediterranean countries is presented, where SGE systems and DHC networks of any kind show a particularly low deployment compared to the rest of Europe. To this end, the second part of this work evaluates, justifies, and analyzes the possibilities and potentialities of their application in this zone.

Autonomous Soft Robots Empowered by Chemical Reaction Networks.

Hydrogel actuators are important for designing stimuli-sensitive soft robots. They generate mechanical motion by exploiting compartmentalized (de)swelling in response to a stimulus. However, classical switching methods, such as manually lowering or increasing the pH, cannot provide more complex autonomous motions. By coupling an autonomously operating pH-flip with programmable lifetimes to a hydrogel system containing pH-responsive and non-responsive compartments, autoonenomous forward and backward motion as well as more complex tasks, such as interlocking of "puzzle pieces" and collection of objects are realized. All operations are initiated by one simple trigger, and the devices operate in a "fire and forget" mode. More complex self-regulatory behavior is obtained by adding chemo-mechano-chemo feedback mechanisms. Due to its simplicity, this method shows great potential for the autonomous operation of soft grippers and metamaterials.

Insights into autoregulation of a membrane protein complex by its cytoplasmic domains

Membrane transporters mediate the passage of molecules across membranes and are essential for cellular function. While the transmembrane region of these proteins is responsible for substrate transport, often the cytoplasmic regions are required for modulating their activity. However, it can be difficult to obtain atomic-resolution descriptions of these autoregulatory domains by classical structural biology techniques, especially if they lack a single, defined structure. The betaine permease, BetP, a homotrimer, is a prominent and well-studied example of a membrane protein whose autoregulation depends on cytoplasmic N- and C-terminal segments. These domains sense and transduce changes in K+ concentration and in lipid bilayer properties caused by osmotic stress. However, structural data for these terminal domains is incomplete, which hinders a clear description of the molecular mechanism of autoregulation. Here we used microsecond-scale molecular simulations of the BetP trimer to compare reported conformations of the 45-amino-acid long C-terminal tails. The simulations provide support for the idea that the conformation derived from electron microscopy (EM) data represents a more stable global orientation of the C-terminal segment under downregulating conditions while also providing a detailed molecular description of its dynamics and highlighting specific interactions with lipids, ions, and neighboring transporter subunits. A missing piece of the molecular puzzle is the N-terminal segment, whose dynamic nature has prevented structural characterization. Using Rosetta to generate ensembles of de novo conformations in the context of the EM-derived structure robustly identifies two features of the N-terminal tail, namely 1) short helical elements and 2) an orientation that would confine potential interactions to the protomer in the counterclockwise direction (viewed from the cytoplasm). Since each C-terminal tail only contacts the protomer in the clockwise direction, these results indicate an intricate interplay between the three protomers of BetP in the downregulated protein and a multidirectionality that may facilitate autoregulation of transport.

International Water Law’s Role in Addressing the Problem of Marine Plastic Pollution: A Vital Piece in a Complex Puzzle!

Abstract The management and protection of water resources, and thus the practice of water law, is absolutely central to every aspect of human well-being. This is as true at the international level as at the domestic level. Water pervades every area of human activity and connects almost every form of pollution and environmental harm. This truism is clearly illustrated by the highly topical problem of marine plastic pollution (MPP) which, though it concerns the ubiquitous use and eventual accumulation in the marine environment of a broad category of environmentally persistent synthetic materials, is intrinsically linked to the environmental management of major rivers, many of which are shared international watercourses. While the general international obligation to prevent marine pollution by plastics can be derived from both international marine environmental law and international water law, evolving practice under the latter framework, and under related sub-fields of international and transnational environmental law, provides helpful clarity regarding the normative parameters of the due diligence standard of conduct expected of all States in this regard.

Anderson-Fabry Disease: A New Piece of the Lysosomal Puzzle in Parkinson Disease?

Anderson-Fabry disease (AFD) is an inherited lysosomal storage disorder characterized by a composite and multisystemic clinical phenotype and frequent involvement of the central nervous system (CNS). Research in this area has largely focused on the cerebrovascular manifestations of the disease, and very little has been described about further neurological manifestations, which are known in other lysosomal diseases, such as Gaucher disease. In particular, a clinical and neuroimaging phenotype suggesting neurodegeneration as a putative mechanism has never been fully described for AFD, but the increased survival of affected patients with early diagnosis and the possibility of treatment have given rise to some isolated reports in the literature on the association of AFD with a clinical phenotype of Parkinson disease (PD). The data are currently scarce, but it is possible to hypothesize the molecular mechanisms of cell damage that support this association; this topic is worthy of further study in particular in relation to the therapeutic possibilities, which have significantly modified the natural history of the disease but which are not specifically dedicated to the CNS. In this review, the molecular mechanisms underlying this association will be proposed, and the available data with implications for future research and treatment will be rewritten.

The social driving forces of desertification in the high Algerian steppe plains

The sedentarization of shepherds at the margins of the desert and the introduction of private land ownership in the high Algerian steppe plains has led to profound lifestyle transformations and increased desertification. Based on policy analysis, field observations and oral surveys with 188 household heads, this paper underlines the social drivers of desertification where short-term economic strategies and political misunderstanding are the key elements. Agro-forestry-pastoral practices are no longer in balance with the local environment. Assembling the puzzle pieces has underlined that political leaders and administrators are only partially familiar with the local geosystem, have implemented multiple unsuccessful measures, and have been unable to sufficiently learn (from their efforts) due to political instability. The paper shows that the sedentarization occurring in this fragile environment with low soil fertility creates rural systems that are less resilient to climate fluctuations, which severely impacts both the environment and the most vulnerable inhabitants.

Endogenous Modulation of Extracellular Matrix Collagen during Scar Formation after Myocardial Infarction

Myocardial infarction is remains the leading cause of death in developed countries. Recent data show that the composition of the extracellular matrix might differ despite similar heart function and infarction sizes. Because collagen is the main component of the extracellular matrix, we hypothesized that changes in inflammatory cell recruitment influence the synthesis of different collagen subtypes in myofibroblasts, thus changing the composition of the scar. We found that neutrophils sustain the proliferation of fibroblasts, remodeling, differentiation, migration and inflammation, predominantly by IL-1 and PPARγ pathways (n = 3). They also significantly inhibit the mRNA expression of fibrillar collagen, maintaining a reduced stiffness in isolated myofibroblasts (n = 4-5). Reducing the neutrophil infiltration in CCR1-/- resulted in increased mRNA expression of collagen 11, moderate expression of collagen 19 and low expression of collagen 13 and 26 in the scar 4 weeks post infarction compared with other groups (n = 3). Mononuclear cells increased the synthesis of all collagen subtypes and upregulated the NF-kB, angiotensin II and PPARδ pathways (n = 3). They increased the synthesis of collagen subtypes 1, 3, 5, 16 and 23 but reduced the expression of collagens 5 and 16 (n = 3). CCR2-/- scar tissue showed higher levels of collagen 13 (n = 3), in association with a significant reduction in stiffness (n = 4-5). Upregulation of the inflammation-related genes in myofibroblasts mostly modulated the fibrillar collagen subtypes, with less effect on the FACIT, network-forming and globular subtypes (n = 3). The upregulation of proliferation and differentiation genes in myofibroblasts seemed to be associated only with the fibrillar collagen subtype, whereas angiogenesis-related genes are associated with fibrillar, network-forming and multiplexin subtypes. In conclusion, although we intend for our findings to deepen the understanding of the mechanism of healing after myocardial infarction and scar formation, the process of collagen synthesis is highly complex, and further intensive investigation is needed to put together all the missing puzzle pieces in this still incipient knowledge process.

The Role of Phialocephala fortinii in Improving Plants' Phosphorus Nutrition: New Puzzle Pieces.

Plants' mineral nutrition in acidic soils can be facilitated by phosphate solubilizing fungi inhabiting the root systems of these plants. We attempt to find dark septate endophyte (DSE) isolates in the roots of wild-heather plants, which are capable of improving plants' phosphorus nutrition levels. Bright-field and confocal laser scanning microscopy were used for the visualization of endophytes. A model system of co-cultivation with Vaccinium macrocarpon Ait. was used to study a fungal isolate's ability to supply plants with phosphorus. Fungal phytase activity and phosphorus content in plants were estimated spectrophotometrically. In V. vitis-idaea L. roots, we obtained a Phialocephala fortinii Wang, Wilcox DSE2 isolate with acid phytase activity (maximum 6.91 ± 0.17 U on 21st day of cultivation on potato-dextrose broth medium) and the ability to accumulate polyphosphates in hyphae cells. The ability of the isolate to increase both phosphorus accumulation and biomass in V. macrocarpon is also shown. The data obtained for the same isolate, as puzzle pieces put together, indicate the possible mediation of P. fortinii DSE2 isolate in the process of phosphorus intake from inorganic soil reserves to plants.

Correlating CO Coverage and CO Electroreduction on Cu via High-Pressure in Situ Spectroscopic and Reactivity Investigations.

The absolute coverage of CO has been a missing piece in the mechanistic puzzle of the CO reduction reaction (CORR) on Cu. For the first time, we revealed the upper bound of the CO coverage under electrocatalytic conditions to be 0.05 monolayer at atmospheric pressure and the saturation CO coverage to be ∼0.25 monolayer by conducting surface enhanced infrared spectroscopy at CO pressures up to 60 barg in a custom-designed spectroelectrochemical cell. CORR activities on Cu were also determined in the same pressure range. Calculated reaction orders of C2+ products with respect to adsorbed CO are substantially less than unity, clearly indicating that the coupling of adsorbed CO is not the rate-determining step leading to multicarbon products. The increase in CO coverage can reduce the C affinity on the Cu surface and favor the selectivity towards oxygenates, especially acetate, over ethylene. Uncommon products, including ethane, glycolaldehyde, and ethylene glycol, were detected in appreciable amounts, likely due to a new C-C coupling mechanism taking place at elevated CO pressures.

On the mechanisms underlying activation and reversal of high altitude-induced pulmonary hypertension in humans - Another piece in the pulmonary puzzle.

On the mechanisms underlying activation and reversal of high altitude-induced pulmonary hypertension in humans - Another piece in the pulmonary puzzle.

Is myocardial work the piece of puzzle that we missed?

Is myocardial work the piece of puzzle that we missed?

Review: Art and Material Culture in the Byzantine and Islamic Worlds: Studies in Honour of Erica Cruikshank Dodd, edited by Evanthia Baboula and Lesley Jessop

Review: <i>Art and Material Culture in the Byzantine and Islamic Worlds: Studies in Honour of Erica Cruikshank Dodd</i>, edited by Evanthia Baboula and Lesley Jessop

WIN Symposium 2022 - Abstract Poster Presentations.

WIN Symposium 2022 - Abstract Poster Presentations.