Picea Rubens Sarg Research Articles

Long-term outcome of precommercial thinning in northwestern New Brunswick: growth and yield of balsam fir and red spruce

A study was established between 1959 and 1961 to study the long-term responses of balsam fir ( Abies balsamea (L.) Mill.) and red spruce ( Picea rubens Sarg.) to precommercial thinning. Three nominal spacings of 4 ft (1.2 m), 6 ft (1.8 m), and 8 ft (2.4 m) were compared with an unthinned control in a randomized complete block design with five replicates. At the time of thinning, natural regeneration averaged 16 years of age, 8 years after harvest. Although thinning had minimal effect on gross total volume production over a 42 to 44 year observation period, actual spacings between 2.1 and 2.5 m produced an average of 360 m3·ha–1gross merchantable volume (GMV), representing a 21% gain over unthinned stands. The same spacings produced quadratic mean diameters of 21 and 23 cm, respectively, compared with 18 cm in the unthinned stands. These size increases translated to individual stem volume gains of 33% and 62%, significantly reducing the age at which thinned stands would meet a specified minimum requirement for merchantability or habitat. The mean annual increment of GMV ranged from 6 m3·ha–1·year–1in unthinned stands, to more than 7 m3·ha·–1·year–1in the thinned stands, and had not yet culminated an average of 50 years postharvest.

Effect of cutting intensity on microenvironmental conditions and regeneration dynamics in yellow birch – conifer stands

This paper presents the 5 year results of different cutting intensities (removal of 0%, 40%, 50%, 60%, and 100% of the basal area) applied in two mixed yellow birch ( Betula alleghaniensis Britt.) – conifer stands of eastern Quebec, Canada. Two sites 90 km apart were used: Armagh and Duchesnay. Each site had four replicates of the treatments in a randomized block design. The effect on light availability was similar in the two sites: the 0%, 40%, 50%, 60%, and 100% cuts transmitting a mean of 5%, 21%, 26%, 30%, and 94% of full light, respectively, during the first summer. Soil temperature increased only in the 100% cut (4−5 °C, maximum daily temperature). Soil disturbance during harvest was higher at Duchesnay than at Armagh, which clearly improved seedbed receptivity, particularly to yellow birch. After 5 years, treated areas contained 21 000 to 48 300 seedlings/ha at Duchesnay compared with 5500 – 10 500 seedlings/ha at Armagh. Significant losses of coniferous advance growth were observed at both sites, but a subsequent seedling recruitment occurred only at Duchesnay. Red spruce ( Picea rubens Sarg.) showed superior establishment in the 60% cut (4400 seedlings/ha) than under other cutting intensities (1600–2100 seedlings/ha), whereas balsam fir ( Abies balsamea (L.) Mill.) responded well to all partial cutting treatments. At both sites, pin cherry ( Prunus pensylvanica L.f.) was the main competing species in the 100% cut, whereas densities of the preestablished mountain maple ( Acer spicatum Lamb.) and striped maple ( Acer pensylvanicum L.) either remained the same or increased in the partial cuts.

Age-related trends in red spruce foliar plasticity in relation to declining productivity

Phenotypic plasticity in needle morphology with increasing tree size and age was investigated by comparing four age classes of red spruce (Picea rubens Sarg.) ranging from juvenile (3-12 years old) to mature (over 100 years old). With increase in tree age there were significant increases in leaf mass per unit area (LMA), mesophyll and vascular bundle area as a percentage of total needle cross-sectional area, and stomatal density. Within the vascular bundle, both xylem cross-sectional area and tracheid lumen area increased significantly, whereas air space as a percentage of total cross-sectional area decreased. These morphological changes were associated with a significant decrease in photosynthetic capacity and stomatal conductance, and an increase in (13)C enrichment. Although both photosynthetic capacity and whole-tree conductance decreased significantly between age classes 3 and 12 years, they did not differ between age classes 53 and 127 years, even though needle (13)C enrichment was significantly greater in the 127-year age class. Thus there appear to be compensatory mechanisms that maintain photosynthetic capacity as trees increase in size and vascular complexity, which in red spruce and other species, may affect leaf hydraulic conductance. Although increased LMA may contribute to reduced photosynthetic capacity in red spruce, similar relationships are not seen in other conifers.

Regeneration development under shelterwoods in a lowland red spruce – balsam fir stand

The shelterwood system can be used to establish regeneration and to improve the growing conditions of seedlings and, thereby, increase their probability of survival after final harvest. To determine the cutting intensity that best promotes the development of red spruce ( Picea rubens Sarg.) regeneration, an experiment, including four repetitions of five treatments (control; low, moderate, and heavy partial cuttings; and clear-cutting), was established in a lowland stand in Quebec, Canada, dominated by red spruce and balsam fir ( Abies balsamea (L.) Mill.). Regeneration development responses to treatments varied according to cutting intensity, species, and position relative to the skidding trails in the residual stands. Ten years after treatment, red spruce seedlings were well established but were smaller than those of balsam fir and deciduous species. Red spruce seedling height was generally greater in partial-cut skidding trails, as was light availability. However, in clearcuts, the size of red spruce seedlings established in skidding trails seemed to be negatively affected by the considerable quantity of woody debris strewn over them. Among the partial-cut treatments, tree regeneration leaf biomass was the highest where 60% of the initial basal area was removed. Thus, this treatment is an attractive alternative to clear-cutting in such lowland stands where watering-up is anticipated after final harvest.

Leaf gas exchange of understory spruce-fir saplings in relict cloud forests, southern Appalachian Mountains, USA

The southern Appalachian spruce-fir (Picea rubens Sarg. and Abies fraseri (Pursh) Poir.) forest is found only on high altitude mountain tops that receive copious precipitation ( > 2000 mm year(-1)) and experience frequent cloud immersion. These high-elevation, temperate rain forests are immersed in clouds on approximately 65% of the total growth season days and for 30-40% of a typical summer day, and cloud deposition accounts for up to 50% of their annual water budget. We investigated environmental influences on understory leaf gas exchange and water relations at two sites: Mt. Mitchell, NC (MM; 35 degrees 45'53'' N, 82 degrees 15'53'' W, 2028 m elevation) and Whitetop Mtn., VA (WT; 36 degrees 38'19'' N, 81 degrees 36'19'' W, 1685 m elevation). We hypothesized that the cool, moist and cloudy conditions at these sites exert a strong influence on leaf gas exchange. Maximum photosynthesis (A(max)) varied between 1.6 and 4.0 micromol CO(2) m(-2) s(-1) for both spruce and fir and saturated at irradiances between approximately 200 and 400 micromol m(-2) s(-1) at both sites. Leaf conductance (g) ranged between 0.05 and 0.25 mol m(-2) s(-1) at MM and between 0.15 and 0.40 mol m(-2) s(-1) at WT and was strongly associated with leaf-to-air vapor pressure difference (LAVD). At both sites, g decreased exponentially as LAVD increased, with an 80-90% reduction in g between 0 and 0.5 kPa. Predawn leaf water potentials remained between -0.25 and -0.5 MPa for the entire summer, whereas late afternoon values declined to between -1.25 and -1.75 MPa by late summer. Thus, leaf gas exchange appeared tightly coupled to the response of g to LAVD, which maintained high water status, even at the relatively low LAVD of these cloud forests. Moreover, the cloudy, humid environment of these refugial forests appears to exert a strong influence on tree leaf gas exchange and water relations. Because global climate change is predicted to increase regional cloud ceiling levels, more research on cloud impacts on carbon gain and water relations is needed to predict future impacts on these relict forests.

Relative role of understory and overstory in carbon and nitrogen cycling in a southern Appalachian spruce–fir forestAES Publication 7863. Utah Agricultural Experiment Station, Utah State University, Logan, Utah.

This study investigated aboveground pools and fluxes of biomass, carbon (C), and nitrogen (N) in the overstory and understory of a southern Appalachian red spruce ( Picea rubens Sarg.) – Fraser fir ( Abies fraseri (Pursh) Poir.) forest, following adelgid-induced fir mortality and spruce windthrow. Using fifty 20 m × 20 m plots, stratified by elevation (1700–1900 m), we estimated standing biomass and fluxes of all growth forms from periodic stand inventories (1998–2003), vegetation surveys, and existing or derived allometric equations. Total C and N pools and fluxes were calculated from plant- and tissue-specific C and N concentrations. Total aboveground biomass attained predisturbance values, ranging from 313 Mg·ha–1at the lower elevations to 204 Mg·ha–1at the upper elevations. Overstory biomass production (5650 kg·ha–1·year–1) and N uptake (11–15 kg·ha–1·year–1) exceeded earlier reported values, indicating forest recovery. Woody understory accounted for 3% of aboveground biomass, 10% of annual productivity, and 19% of total N uptake (∼7 kg·ha–1·year–1). Herbaceous vegetation, which comprised only 1% of total biomass, took up 18–21 kg N·ha–1annually, >50% of total ecosystem N uptake (37 kg·ha–1·year–1). This suggests that N-rich understory vegetation plays an important role in N cycling.

Rove beetles (Coleoptera: Staphylinidae) in yellow birch-dominated stands of southeastern Quebec, Canada: Diversity, abundance, and description of a new species

AbstractRove beetle assemblages from forest dominated by yellow birch (Betula alleghaniensis Britt. (Betulaceae)) at two sites in southeastern Quebec were characterized in terms of composition, diversity, and relative abundance. In total, 143 species were collected over two seasons (June–August of 1999 and 2000). In the pretreatment year (1999), 88 species were found in uncut stands. In the post-treatment year, 116 species were found in treated (by patch harvesting and ground scarification) and control plots combined. Sixty-one species were common to the two sites, 27 species captured in the 1999 season were not recaptured in the 2000 season, and 55 additional species were captured in 2000. There are 23 new distribution records for Quebec, and one species, Atheta (Dimetrota) pseudomodesta Klimaszewski, sp. nov., is described. New bionomics data on relative abundance, seasonality, and the utility of different trapping methods are presented. Assemblage composition is compared with that of a red spruce (Picea rubens Sarg. (Pinaceae)) forest in New Brunswick.

Carbon storage in a chronosequence of red spruce (Picea rubens) forests in central Nova Scotia, Canada

Red spruce ( Picea rubens Sarg.) forests are an ecologically and economically important forest type in eastern Canada. We quantified the carbon (C) stocks of natural red spruce dominated stands in central Nova Scotia. Twenty-four stands over a 140 year chronosequence were sampled. Within each stand, major C pools including above- and below-ground tree biomass, shrub and herb vegetation, dead organic matter, and upper (0–10 cm) mineral soil were measured. A nonlinear four-parameter logistic function was fitted to the total site C stock data to describe the change in total ecosystem C storage over time. Total site C storage increased throughout stand development in a general sigmoidal pattern, increasing from 94.4 Mg C·ha–1in the youngest age-class to a maximum of 247.0 Mg C·ha–1in the 81- to 100-year-old age-class, then decreasing in the oldest age-classes. Carbon pools of live vegetation, standing dead trees, and downed woody debris displayed recognizable changes in C storage throughout stand development, conforming to some of the fundamental ideas on forest stand dynamics. Overall, above- and below-ground tree biomass had the greatest influence on total site C storage dynamics. These results are likely to be integrated into further forest management plans and generalized in other contexts to evaluate carbon stocks at the regional scale.

Comparative nutrient economy, stable isotopes, and related adaptive traits in Picea rubens, Picea mariana, and their hybrids

Nutrient- and water economy-related traits in plants have significant implications for growth and fitness. We explored, examined, and compared nutrient concentrations, use efficiencies, assimilation, and informative isotopic elements in a seedling provenance experiment, and in seedling and mature tree controlled-cross hybrid experiments of red spruce (RS) (Picea rubens Sarg.) and black spruce (BS) (P. mariana (Mill.) BSP). Provenance experiment results showed RS had consistently lower carbon (C) and nitrogen (N) concentrations, and N assimilation ratio (NAR), but higher N-use efficiency (NUE), C:N ratio, water-use efficiency (WUE), and needle calcium (Ca) and magnesium (Mg) concentrations than BS. The hybrid seedling experiment showed similar results and additive inheritance for needle N, C:N ratio, NAR, Ca, and Mg, evident by a near-linear progression from one species to the other. Within both species, seedling height showed a negative relationship with needle N and a positive relationship with NUE. However, across hybrid indices, seedling height showed a positive relationship with needle N and a negative relationship with NUE. Also across hybrid indices, seedling height showed a negative relationship to Ca and C:N, and a positive relationship with NAR and 13C discrimination (without hybrid 25). Mature tree hybrid experiment results were similar to those of the seedling experiment, but with a dampening of differences caused by low nutrient availability and possibly age effects. The similarity was not true for 13C discrimination as mature tree height showed a strong negative relationship to 13C discrimination, indicating that BS had greater WUE. The reversal is most probably caused by the large difference in water availability.

Nitrogen and calcium additions increase forest growth in northeastern USA spruce–fir forests

We determined responses of red spruce ( Picea rubens Sarg.) – balsam fir ( Abies balsamea (L.) Mill.) forests to 6 years of nitrogen (N), calcium (Ca), and N + Ca treatments (100, 160, and 260 kg·ha–1·year–1 of N, Ca, and N + Ca, respectively) in New York (NY) and New Hampshire (NH). Forest responses to Ca treatments were also determined in Vermont (VT). Nitrogen treatments increased aboveground net primary production (ANPP) by 33% and 25% above controls in NY and NH, respectively. Similarly, N + Ca treatments increased ANPP by 27% and 28% in NY and NH, respectively. Calcium treatments increased ANPP by 25% and 21% above controls in NY and VT. Calcium treatment did not increase ANPP in NH, suggesting N, but not Ca limitation. Leaf-litter quantity and quality, and soil C and N storage were greater in treated than in control plots. Fine-root mass and production did not differ among treatments. Trees, therefore, assimilated more soil nutrients without increasing root growth in treated plots. Red spruce ANPP, however, declined or remained unchanged in response to N and Ca additions. The equivalent of 68–102 years of anthropogenic N addition to soils changed forest species composition without decreasing ANPP, and Ca additions did not prevent this change.

Chlorophyll fluorescence response of red spruce and balsam fir to a watershed calcium fertilization experiment in New Hampshire

Decreases in soil exchangeable calcium (Ca) due to acid deposition have been linked with declines of forest species in the northeastern United States, particularly red spruce (Picea rubens Sarg.). In 2005, chlorophyll fluorescence measurements were taken on red spruce and balsam fir (Abies balsamea (L.) Mill.) trees growing on two watersheds at the Hubbard Brook Experimental Forest (HBEF) in New Hampshire. One watershed had been fertilized with CaSiO3 in 1999 to replace the soil Ca losses of the past 50 years, and the other was a reference watershed. In March, there were no differences in any chlorophyll fluorescence parameter between sites or species. In August, however, dark-adapted ratios of variable fluorescence to maximum fluorescence (Fv/Fm) were significantly greater (p = 0.05) in the Ca-treated watershed for both spruce and fir, and spruce values were significantly greater than fir. No differences were found in the light-adapted fluorescence parameters. These results suggest that both spruce and fir respond to increases in Ca availability, even though fir does not exhibit the decline symptoms seen in spruce. It is unclear what physiological effect is responsible for the observed differences in Fv/Fm. The Fv/Fm values appear to respond to differences in Ca availability in forest tree species; thus, the ratio has the potential to be used in these two watersheds at HBEF to determine if other forest species are also responding to Ca additions.

Soil organic carbon dynamics along a climatic gradient in a southern Appalachian spruce–fir forest

A field study was conducted in a high-elevation spruce–fir ( Picea rubens Sarg. – Abies fraseri (Pursh.) Poir) forest in the Great Smoky Mountains National Park to assess the effect of temperature on soil C storage and dynamics. In eight plots along an elevation gradient (1500–1900 m), we measured soil temperature, forest floor and mineral soil C, litter decomposition, soil respiration, and forest floor mean residence time. Mean annual soil temperature and annual degree-days above 5 °C were inversely correlated with elevation. Total soil C (166–241 Mg·ha–1) showed no trend with elevation, while forest floor C accumulation (16.3–35.9 Mg·ha–1) decreased significantly with elevation. Carbon dynamics did not follow a consistent elevation pattern; however, the cooler upper elevations showed the lowest C turnover as indicated by the lowest needle decomposition rate (k = 0.0231·year–1) and the longest mean residence time of forest floor C (22 years). Mean annual CO2efflux from the soil (1020–1830 kg C·ha–1·year–1) was negatively correlated with mean annual soil temperatures and annual degree-days above 5 °C. This gradient study offers useful insights into C release patterns under future warming scenarios, and suggests that the highest elevation may be most susceptible to global warming.

Changes in conifer and deciduous forest foliar and forest floor chemistry and basal area tree growth across a nitrogen (N) deposition gradient in the northeastern US

We evaluated foliar and forest floor chemistry across a gradient of N deposition in the Northeast at 11 red spruce ( Picea rubens Sarg.) sites in 1987/1988 and foliar and forest floor chemistry and basal area growth at six paired spruce and deciduous sites in 1999. The six red spruce plots were a subset of the original 1987/1988 spruce sites. In 1999, we observed a significant correlation between mean growing season temperature and red spruce basal area growth. Red spruce and deciduous foliar %N correlated significantly with N deposition. Although N deposition has not changed significantly from 1987/1988 to 1999, net nitrification potential decreased significantly at Whiteface. This decrease in net potential nitrification is not consistent with the N saturation hypothesis and suggests that non-N deposition controls, such as climatic factors and immobilization of down dead wood, might have limited N cycling.

A variance-covariance structure to take into account repeated measurements and heteroscedasticity in growth modeling

This study proposes a within-subject variance-covariance (VC) structure to take into account repeated measurements and heteroscedasticity in a context of growth modeling. The VC structure integrates a variance function and a continuous autoregressive covariance structure. It was tested on a nonlinear growth model parameterized with data from permanent sample plots. Using a stand-level approach, basal area growth was independently modeled for red spruce (Picea rubens Sarg.) and balsam fir [Abies balsamea (L.) Mill.] in mixed stands. For both species, the implementation of the VC structure significantly improved the maximum likelihood of the model. In both cases, it efficiently accounted for heteroscedasticity and autocorrelation, since the normalized residuals no longer exhibited departures from the assumptions of independent error terms with homogeneous variances. Moreover, compared with traditional nonlinear least squares (NLS) models, models parameterized with this VC structure may generate more accurate predictions when prior information is available. This case study demonstrates that the implementation of a VC structure may provide parameter estimates that are consistent with asymptotically unbiased variances in a context of nonlinear growth modeling using a stand-level approach. Since the variances are no longer biased, the hypothesis tests performed on the estimates are valid when the number of observations is large.

Management for red spruce conservation in Québec: The importance of some physiological and ecological characteristics – A review

The physiological and ecological characteristics of red spruce (Picea rubens Sarg.) are reviewed and integrated into ecosystem management options. Red spruce is a shade-tolerant, late-successional conifer species found in the temperate forests of northeastern North America. Its wood, being of excellent quality, is prized by the forest industry. Unfortunately, this high-value species has been in decline throughout its entire range for the past 50 years. At high elevations, in northeastern United States, crown dieback caused by the combined effect of atmospheric pollution and climate is largely responsible of this decline. In other areas, such as Québec (Canada), the scarcity of red spruce is mainly caused by forest management practices that are poorly adapted to the species' ecophysiology. Many physiological studies have shown that the species is sensitive to full sunlight (at juvenile and advance growth stages), high temperatures and frost. It also has particular microsite requirements for seed germination and early seedling establishment, such as the presence of large decaying woody debris. Hence, a management strategy adapted for red spruce should favour the use of partial cutting, maintaining some overstory and dead wood. This will emulate the natural dynamics of small canopy gaps and minimize the physiological stresses to regeneration. The ecophysiological aspects of natural and artificial regeneration of red spruce should be examined with respect to the increased use of partial cutting techniques. Key words: advance regeneration, balsam fir (Abies balsamea L.), ecophysiology, ecosystem management, frost susceptibility, light response, microenvironment, red spruce (Picea rubens Sarg.), seedling establishment, shade tolerance, silvicultural systems, thermosensitivity

Concentrations of Ca and Mg in early stages of sapwood decay in red spruce, eastern hemlock, red maple, and paper birch

The decay of coarse woody debris is a key component in the formation of forest soil and in the biogeochemical cycles of Ca and Mg. We tracked changes in density and concentration of Ca and Mg in sapwood of red maple ( Acer rubrum L.), red spruce ( Picea rubens Sarg.), paper birch ( Betula papyrifera Marsh.), and eastern hemlock ( Tsuga canadensis (L.) Carr.) in Maine and New Hampshire. We repeatedly sampled 10 logs of each combination of tree species and location at the time of felling and at 2-year intervals for 6 years (birch and hemlock) or 8 years (spruce and maple). We found that density loss was essentially linear for the time period investigated, with birch and maple sapwood decaying at faster rates than spruce and hemlock. Repeated-measures analysis and regression modeling of log-transformed concentrations indicated a significant accumulation of Ca for sapwood of all tree species at both locations (30%–90% increase after 6–8 years of ground contact). Regression estimates of Mg concentration in spruce and maple declined about 20% during the 8 years of ground contact. There was no significant trend for Mg concentration in birch and hemlock. Variation in decay rates and trends in Ca and Mg concentration may be due to differences in sapwood quality, the community of wood decay fungi and associated organisms, or to abiotic conditions.

A stand density management diagram for spruce–balsam fir mixtures in New Brunswick

Balsam fir (Abies balsamea (L.) Mill.), red spruce (Picea rubens Sarg.), black spruce (P. mariana (Mill.) BSP), and white spruce (P. glauca (Moench) Voss) often form mixed stands throughout northeastern North America. After harvesting operations or natural disturbances, the resulting natural regeneration may require thinning prescriptions to achieve the desired future stand structure and associated forest products. Stand density management diagrams (SDMDs) can assist the forest manager in examining potential yield implications of stand density management decisions. Data from New Brunswick, Nova Scotia, and Quebec indicate a single SDMD is not appropriate for mixtures of balsam fir and spruce (red and black). The maximum size density line is flatter for mixtures than for pure species stands and the quadratic mean diameter isolines are affected by the species composition. The top height isolines are independent of species composition. The results indicate the SDMD for spruce–balsam fir mixtures needs to be dynamic, incorporating the species proportions. The SDMD has been incorporated into software that prompts the user for the balsam fir fraction and generates the appropriate SDMD. Key words: Acadian Forest Region, eastern species mixtures, thinning decisions

Dendrochronological reconstruction of spruce budworm outbreaks in northern Maine, USA

Using dendrochronological analyses, we reconstructed a 300 year history of eastern spruce budworm ( Choristoneura fumiferana (Clem.)) outbreaks in northern interior Maine. By analyzing radial growth patterns from the budworm host, red spruce ( Picea rubens Sarg.), and nonhost, northern white cedar ( Thuja occidentalis L.), we identified five outbreaks beginning ca. 1709, 1762, 1808, 1914, and 1976, all of which have been documented from eastern Canada. However, little or no evidence was found in our study for the 1830s, 1870s, or 1940s outbreaks also documented there. The mean outbreak return interval in our study (67 years) was roughly twice that postulated for eastern Canada. Differences in forest types, and associated stand dynamics, between the regions may explain the longer return intervals, and consequently the absence of these three outbreaks in Maine. Results also indicate that small, slow-growing trees exhibit a budworm signal very similar to that of overstory trees, once tree-ring series have been properly standardized.

Genetic variation and control of chloroplast pigment concentrations in Picea rubens, Picea mariana and their hybrids. I. Ambient and elevated [CO2] environments

Traits related to light-energy processing have significant ecological implications for plant fitness. We studied the effects of elevated atmospheric CO(2) concentration ([CO(2)]) on chloroplast pigment traits of a red spruce (RS) (Picea rubens Sarg.)-black spruce (BS) (P. mariana (Mill.) B.S.P.) genetic complex in two experiments: (1) a comparative species' provenance experiment from across the near-northern part of the RS range; and (2) an intra- and interspecific controlled-cross experiment. Results from the provenance experiment showed that total chlorophyll (a + b) concentration was, on average, 15% higher in ambient [CO(2)] than in elevated [CO(2)] (P < 0.001). In ambient [CO(2)], BS populations averaged 11% higher total chlorophyll and carotenoid concentrations than RS populations (P < 0.001). There were significant species, CO(2), and species x CO(2) interaction effects, with chlorophyll concentration decreasing about 7 and 26% for BS and RS, respectively, in response to elevated [CO(2)]. Results from the controlled-cross experiment showed that families with a hybrid index of 25 (25% RS) had the highest total chlorophyll concentrations, and families with hybrid indices of 75 and 100 had among the lowest amounts. Initial analysis of the controlled-cross experiment supported a more additive model of inheritance; however, parental analysis showed a significant and predominant male effect for chlorophyll concentration. In ambient and elevated [CO(2)] environments, crosses with BS males had 10.6 and 17.6% higher total chlorophyll concentrations than crosses with hybrid and RS males, respectively. Our results show that chlorophyll concentration is under strong genetic control, and that these traits are positively correlated with productivity within and across species. A significant positive correlation between chlorophyll concentration and the ratio of total plant N to root dry mass was also found (r = 0.872). The almost fourfold decrease in chlorophyll concentration in RS suggests that it would be at a competitive disadvantage compared with BS in a high [CO(2)] environment.

Algae Associated with Myxomycetes and Leafy Liverworts on Decaying Spruce Logs

ABSTRACT Algae associated with leafy liverworts growing on decorticated red spruce (Picea rubens Sarg.) logs were investigated at study sites in the mountains of West Virginia and the Great Smoky Mountains National Park. Twelve taxa (nine cyanobacteria, two green algae and one diatom) were identified, but the microhabitat represented by the samples we examined was dominated by just three taxa, two cyanobacteria (Chroococcus tenax and Aphanothece saxicola) along with the green alga Chlorococcum humicola. The relative abundance and consistent presence of these organisms in this microhabitat suggest that they could represent a possible food source for certain species of myxomycetes with which they co-occur.