The Ascochyta Blight (AB) caused by Ascochyta rabiei is recognized as a major foliar disease of chickpea worldwide. The rapid detection of AB is a prerequisite for the effective and timely management of disease at an early stage to prevent the AB epidemics in chickpea. The Indian isolates of A. rabiei were characterized pathogenically and sequencing of Internal Transcribed Spacer (ITS) region. The phylogenetic analysis of ITS sequences indicated 99.97% similarity index and isolates formed two major clusters through neighbour joining analysis. The RAPD monomorphic bands were cloned and sequenced. The best set of SCAR marker pairs designated as SCAR1F/SCAR1R were validated using PCR with sensitivity of 0.30 ng of genomic DNA. The RAPD derived SCAR marker demonstrated high sensitivity and were species specific in the detection of A. rabiei. The SCAR marker could be used for quick and timely detection of the A. rabiei fungus in seed materials of quarantine importance for phytosanitary certification of chickpea.