A new phytoecdysteroid, punisterone [(20R,24S)-25-deoxy-11α,20,24-trihydroxyecdysone] (1), together with six known ones, ecdysone, 20-hydroxyecdysone, 5β,20-dihydroxyecdysone, ponasterone C, pterosterone, and turkesterone, have been isolated from the seeds of Blandfordia punicea.