Extraction Of Phytochemicals Research Articles

Green Extraction of Phytochemicals from Fresh Vegetable Waste and Their Potential Application as Cosmeceuticals for Skin Health

The utilization of bioactive compounds from fresh produce waste, which is gaining attention in the agri-food and cosmetics industries, focuses on employing green extraction over conventional extraction methods. This emerging field addresses environmental concerns about food waste and the uses of bioactive phytochemicals for skin health. Modern green extraction methods aim to minimize the energy-intensive process and the use of harmful solvents. These techniques include ultrasound, microwave, and supercritical fluid extraction, pulsed electric field extraction, pressurized liquid extraction, and subcritical water extraction methods, which provide high efficacy in recovering bioactive phytochemicals from vegetable and root crops. The phytochemicals, such as carotenoids, polyphenols, glucosinolates, and betalains of fresh produce waste, exhibit various therapeutic properties for applications in skin health. These dietary antioxidants help to neutralize free radicals generated by UV radiation, thus preventing oxidative stress, DNA damage, and inflammation. The skin care formulations with these phytochemicals can serve as natural alternatives to synthetic antioxidants that may have toxic and carcinogenic effects. Therefore, this review aims to discuss different green extraction technologies, consumer-friendly solvents, and the beneficial skin health properties of selected phytochemicals. The review highlights recent research on major phytochemicals extracted from vegetables and root crops in relation to skin health.

Prunella vulgaris-phytosynthesized platinum nanoparticles: Insights into nanozymatic activity for H2O2 and glutamate detection and antioxidant capacity

Artificial nanozymes (enzyme-mimics), specifically metallic nanomaterials, have garnered significant attention recently due to their reduced preparation cost and enhanced stability in a wide range of environments. The present investigation highlights, for the first time, a straightforward green synthesis of biogenic platinum nanoparticles (PtNPs) from a natural resource, namely Prunella vulgaris (Pr). To demonstrate the effectiveness of the phytochemical extract as an effective reducing agent, the PtNPs were characterized by various techniques such as UV–vis spectroscopy, High-resolution Transmission electron microscopy (HR-TEM), zeta-potential analysis, Fourier-transform infrared spectroscopy (FTIR), and Energy dispersive spectroscopy (EDS). The formation of PtNPs with narrow size distribution was verified. Surface decoration of PtNPs was demonstrated with multitudinous functional groups springing from the herbal extract. To demonstrate their use as viable nanozymes, the peroxidase-like activity of Pr/PtNPs was evaluated through a colorimetric assay. Highly sensitive visual detection of H2O2 with discrete linear ranges and a low detection limit of 3.43 μM was demonstrated. Additionally, peroxidase-like catalytic activity was leveraged to develop a colorimetric platform to quantify glutamate biomarker levels with a high degree of selectivity, the limit of detection (LOD) being 7.00 μM. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) test was used to explore the scavenging nature of the PtNPs via the degradation of DPPH. Overall, the colorimetric assay developed using the Pr/PtNP nanozymes in this work could be used in a broad spectrum of applications, ranging from biomedicine and food science to environmental monitoring.

Role of infrared-assisted extraction of antioxidant phytochemicals in enhancing antimicrobial activity of Phaeodactylumtricornutum

Microalgae are a sustainable source of highly bioactive and nutritious biomolecules such as polyphenols, and carotenoids. The efficiency of a novel infrared (IR) technology in terms of optimization of phytochemical (phenolic compounds, chlorophylls and carotenoids) recovery from P. tricornutum was studied using Response Surface Methodology (RSM). Optimized antioxidant activity (870 ± 22 μg TE/mL) and phenolic compound concentration (562 ± 7 mg GAE/100 g DM) were obtained using IR at 33 °C for 12 min at pH 7. In addition, chlorophyll A (26.54 ± 2.78 mg/100 g DM) and carotenoids (9.61 ± 0.40 mg/100 g DM) were recovered at the simultaneous optimal IR conditions: 56 °C, 36 min, pH 9.5. Compared to conventional aqueous extraction, IR enhanced by 10.06% the recovery of phenolic compounds and ameliorated their biological activities by 36.24% for DPPH, 17.24% for CUPRAC, 14.50% for TEAC, and 4.60% for FRAP. Finally, IR bioactive extracts also exhibited antibacterial effects against S. aureus. In conclusion, the richness of P. tricornutum in phytochemicals makes it a valuable alternative food source. IR technology proved to be an efficient method for the recovery of high-added value compounds from microalgae. Antioxidant and pigment-rich microalgae extracts can be used as dietary supplements.

Green Synthesis of Ag/Mg(OH)2 Nanocomposite from Potato Peel Extract (Solanum tuberosum) and Its Antioxidant Activity

Potato peel extract (PPE) is a rich source of polyphenols which could be used as reducing agents for the green synthesis of nanocomposites. The project aims to synthesize Ag/Mg(OH)2 nanocomposites (NCs) by using PPE and investigate its antioxidant activity. The phytochemicals in peel extract act as reducing, stabilizing, and capping agents in the reduction of precursor salts. SEM-EDX analyses of the bio-synthesized Ag/Mg(OH)2 NCs show the formation of Ag nanoparticles (NPs) on the surface of Mg(OH)2 carriers with various sizes in the nanoscale, while XRD patterns suggest the existence of Mg(OH)2 hexagonal crystalline structure. The antioxidant activity of Ag/Mg(OH)2 NCs was assessed through the DPPH free radical scavenging assay with IC50 values between 1.3 and 1.4 mg/mL. Compared to various plant-based Ag NPs, such values are remarkably closer to the IC50 of the standard ascorbic acid (0.6 mg/mL), promoting PPE-based Ag/Mg(OH)2 as an effective and ecofriendly antioxidant.

Phytochemical analysis, Antioxidant and Antimicrobial Screening of Shoot Extracts of Viola odorata linn. from Shikari Devi Wildlife Sanctuary, Himachal Pradesh, India

The traditional healers made significant use of the Viola odorata of the Violaceae family for its many ethnopharmacological uses. The phytochemical composition and in vitro antioxidant activity of solvent extracts from Viola odorata shoots were studied, and the antibacterial potential of the optimized extract was also examined. The Soxhlet apparatus was used to extract phytochemicals from shoots in four different solvents, including n-hexane, butanol, methanol, and water. High-performance liquid chromatography (HPLC) was used to analyze the stigmasterol profile of V. odorata shoots. The extracts were found to contain alkaloids, flavonoids, terpenoids, saponins, and phenolics, all of which were found in significant amounts in the methanolic extract. The methanolic extract also showed the highest radical scavenging activity (79.23 ± 0.44 %). TLC and HPLC profiling of methanolic extract revealed a considerable amount of stigmasterol in the shoots of Viola odorata. HPLC analysis of the methanolic shoot extract of the plant revealed the presence of 0.009 µg mL-1 stigmasterol. Optimized Methanolic extract showed respectable in-vitro antimicrobial activity against Bacillus subtilis (2.6 ± 0.41 mm zone of inhibition), E. coli (3.4 ± 0.71 mm zone of inhibition), and Candida albicans (3.5 ± 0.48 mm zone of inhibition). The outcomes demonstrated that the methanolic shoot extract of Viola odorata from Shikari Devi Wildlife Sanctuary, Himachal Pradesh, India, is a valuable herb for further screening and might be prepared for in-vivo animal studies.

Comparative Phytochemical Constituents of Extracts of Bryophyllum pinnatum Grown in Anambra State, Nigeria

Bryophyllum pinnatum is a plant abundant in several phytochemicals, that can be extracted, purified, and packaged for the goal of promoting public health. In this study, the phytochemical composition of the leaf extracts of Bryophyllum pinnatum grown in Anambra State, Nigeria, was compared. B. pinnatum leaves were obtained from a farm in Anambra State, Nigeria. The leaves were processed and extracted using cold water and hot water extraction, methanol extraction, and ethanol extraction. The extraction processes were carried out following standard extraction methods. Preliminary qualitative and quantitative analyses were conducted to determine the presence and concentration of phytochemicals in the plants. Phytochemicals tested include alkaloids, flavonoids, saponins, tannins, anthocyanins, reducing sugars, carbohydrates, proteins, cardiac glycosides, terpenoids, and phlobatannins. All the tested phytochemicals were recovered in the solvent (methanol and ethanol) extracts; carbohydrates, reducing sugar, protein, and anthocyanin were absent in the aqueous (cold and hot water) extracts. Varying concentrations of the phytochemicals in the different extracts were recorded, and they range from 0.00±0.00% to 13.30±0.22%, with the highest concentration of the extracts recovered in the solvent (methanol and ethanol) extracts. This study revealed that the extraction method used can influence the type and concentration of phytochemicals recovered in Bryophyllum pinnatum. Therefore, the specific desired phytochemical should determine the extract method to use in any study. Int. J. Appl. Sci. Biotechnol. Vol 12(1): 1-7.

Comparison of the Efficiency of Deep Eutectic and Organic Solvents in the Extraction of Phytochemicals from Cannabis sativa L.

Industrial hemp (Cannabis sativa L.) is an attractive candidate for sustainable pest management due to its abundance of bioactive compounds with potential pesticidal properties. Solvent choice has a significant impact on the extraction efficiency of bioactive compounds. Deep Eutectic Solvents (DESs) are gaining popularity in extraction because they are safe and environmentally friendly, making them viable alternatives to organic solvents (OSs). This research first compared the extraction efficiency of OSs in the extraction of phytochemicals from the infloresences of two hemp varieties, Citrus and Cherry Dwarf. Inflorescences were extracted using three OSs, ethanol, ethyl acetate, and hexane. The highest level of cannabidiol (CBD; 0.69%) was extracted from Cherry Dwarf using ethanol, while the level of delta-9 tetrahydrocannabinol THC (0.19%) was essentially the same in both. Therefore, Cherry Dwarf was selected to compare the extraction efficiency of DESs with OSs. The DESs were choline chloride/ethylene glycol, citric acid/ethylene glycol, menthol/lauric acid, choline chloride/urea, and choline chloride/glycerol. In the targeted analysis, choline chloride/ethylene glycol extracted the highest amount of CBD (0.87%) followed by choline chloride/urea (0.78%). As some DESs outperformed ethanol, the popular solvent for extracting cannabinoids, DESs are viable candidates for replacement of organic solvents.

Bioprospecting of Vernonia cinerea for nutraceutical and homemade first aid remedies: a review update

Vernonia cinerea (VC) is a ubiquitous weed in tropical and subtropical regions. It was traditionally used to treat health issues by various cultures, but today little is known to the new generation about its high medicinal values, how to use it as a quick first aid, and its commercialization potential. A literature search found that VC has been extracted using various kinds of solvents for different polarities of phytochemical extraction. Interestingly, the food-grade solvents such as ethanol and water were found to be suitable to extract the active ingredients from VC for major therapeutic applications such as antioxidants, anti-diabetic, anti-inflammatory, anti-cancer, and anti-microbial as well as being a wound healer, hepato-protective, and ease the smoking withdrawals symptoms. The spray-dried water extracts are currently innovated into different forms of nutraceutical products. The high toxicity threshold allows the utilisation of VC as home remedies in the form of fresh, dried, and water extracts for oral intake, while the extracts using common alcohol/spirit and oil can be made for topical applications. This review aimed to give an insight into the commercialization potential of VC as a nutraceutical product and the reestablishment of homemade first-aid remedies for various health conditions especially among the people in rural areas.

Chemotherapeutic prospects of organic extracts of Bornetella nitida from Selayar Island

In this study, we aimed to identify the chemotherapeutic potential of Bornetella nitida, a Dasycladacean that contains various phytochemicals. Phytochemical extraction was performed using gradient maceration with n-hexane, chloroform, ethyl acetate, and methanol. Additionally, toxicity tests as well as antioxidant activity, cytotoxicity, phytochemical, and molecular docking analyses against three main cervical cancer proteins were performed. The B. nitida extracts showed moderate toxicity with LC50 ranging from 116.06 to 713.12 μg mL−1. The free-radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl varied with solvent polarity, exhibiting IC50 values of 22.20, 87.77, 401.55, and 752.08 μg mL−1 when extracted using n-hexane, chloroform, ethyl acetate, and methanol, respectively. Meanwhile, the cytotoxicity against HeLa cells ranged from 281.09 to 1329.33 μg mL−1, with the highest activity shown by the ethyl acetate extract. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of phytochemicals with various bioactivities in the extract, such as L-ascorbic acid 2,6-dihexadecanoate, octadec-9-enoic acid, hexadecatrienoic acid, 2,4-bis(1,1-dimethylethyl)phenol, 9,12-octadecadienoic acid (Z,Z), 1,2-benzenedicarboxylic acid, 2,6,10-trimethyl-14-ethylene-14-pentadecene, and phytol. Docking studies implied that the compounds commonly interact well with the binding site of the target protein. Overall, our results indicate that B. nitida extracts especially ethyl acetate extract can be developed as chemotherapeutic agents.

Tea Grounds as a Waste Biofiller for Natural Rubber.

The aim of this study was the utilization of ground tea waste (GT) left after brewing black tea as a biofiller in natural rubber (NR) composites. Ionic liquids (ILs), i.e., 1-ethyl-3-methylimidazolium lactate and 1-benzyl-3-methylimidazolium chloride, often used to extract phytochemicals from tea, were applied to improve the dispersibility of GT particles in the elastomeric matrix. The influence of GT loading and ILs on curing characteristics, crosslink density, mechanical properties, thermal stability and resistance of NR composites to thermo-oxidative aging was investigated. The amount of GT did not significantly affect curing characteristics and crosslink density of NR composites, but had serious impact on tensile properties. Applying 10 phr of GT improved the tensile strength by 40% compared to unfilled NR. Further increasing GT content worsened the tensile strength due to the agglomeration of biofiller in the elastomer matrix. ILs significantly improved the dispersion of GT particles in the elastomer and increased the crosslink density by 20% compared to the benchmark. Owing to the poor thermal stability of pure GT, it reduced the thermal stability of vulcanizates compared to unfilled NR. Above all, GT-filled NR exhibited enhanced resistance to thermo-oxidation since the aging factor increased by 25% compared to the unfilled vulcanizate.

Exploring the Phytochemical Diversity and Antioxidant Potential of the Vietnamese Smilax glabra Roxb: Insights from UPLC-QTOF-MS/MS and Zebrafish Model Studies.

Research on natural products is growing due to their potential health benefits and medicinal properties. Despite regional variations in phytochemical composition and bioactivity, Smilax glabra Roxb (SGB) has attracted the interest of researchers. Scientists are particularly interested in the Vietnamese SGB variant, which is influenced by biological and environmental factors. Despite geographical differences in phytochemical makeup and bioactivities, SGB remains a fascinating subject in traditional herbal medicine. Using ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS), the phytochemicals in Vietnamese SGB extracts were investigated. This study revealed a wide range of phytochemical compounds, including flavonoids, terpenoids, glycosides, alkaloids, organic acids, phenolics, and steroids. Furthermore, utilizing zebrafish as a model organism, we discovered that these extracts have the surprising ability to greatly improve the survival rate of zebrafish larvae exposed to oxidative stress caused by arsenite (NaAsO2) and hydrogen peroxide (H2O2). Notably, our discoveries suggest the occurrence of new antioxidative pathways in addition to the kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, expanding the understanding of the antioxidant properties and potential therapeutic uses of these plants. To summarize, our research findings shed light on the phytochemical composition of Vietnamese SGB, revealing its potential as a natural antioxidant and encouraging further exploration of its underlying mechanisms for future innovative antioxidant therapies.

In Vitro and In Silico Screening For Potential Phytochemicals In Alcoholic Extract Of Cheilocostus Speciosus For Its Pharmacological Properties

In Vitro and In Silico Screening For Potential Phytochemicals In Alcoholic Extract Of Cheilocostus Speciosus For Its Pharmacological Properties

Comparative antioxidant analysis of extracts prepared from Indigofera longiracemosa aerial parts identifies leaf methanolic extract as a promising antioxidant

Extraction of phytochemicals from the leaves and stems of Indigofera longiracemosa, a previously underexplored dye-yielding plant, was done using four solvents of increasing polarity followed by in vitro assessment of their antioxidant potential. Of the four solvent extracts, methanol extract yielded the highest percentage of phytochemicals. Methanol extracts of leaves and stems also showed the best antioxidant potential in in vitro antioxidant assays. Further, comparison with known standard antioxidant molecules showed lower IC50 values for the extracts tested. Our study thereby validates for the first time the antioxidant potential of I. longiracemosa aerial parts, and identifies an extract enriched in phytochemicals capable of quenching free radicals in vitro at concentrations lower than or comparable to pure antioxidant molecules.

Phytochemical Analysis of Different Plant Extracts and their Inhibitory Effects on Aspergillus niger and Fusarium solani

Plant extracts constitutes versatile phytochemicals which can be substituted with commercial synthetic chemicals to inhibit the growth of pathogens. In this study, the methanol and aqueous extracts of guava, jackfruit, tulsi, peppermint and eucalyptus leaves were evaluated in-vitro against Aspergillus niger and Fusarium solani isolated from Pomegranate and Guava respectively. Plant extracts were prepared using Soxhlet apparatus and condensed the extract by rotary evaporation. Highest yield was obtained in methanol plant extracts compared to aqueous extract, as it causes differences in the polarity of the extraction which leads to wide variation in the level of bioactive compounds in the extract. Poison food technique was used to test the antifungal activity of plant extracts. Jackfruit leaves methanol extract at 7000ppm showed maximum inhibition of 88.89% and guava leaves aqueous extract at 3000ppm showed minimum inhibition of 48.15% against Aspergillus niger. In case of Fusarium solani maximum inhibition was observed in jackfruit leaves methanol extract at 7000ppm of 87.89% and minimum inhibition was recorded in eucalyptus leaves aqueous extract of 57.41% at 3000ppm.The qualitative phytochemical screening of selected plant extracts showed various phytochemicals in plant extracts like tannins, phenols, flavonoids, terpenoid, saponin and alkaloid. GC-MS analysis of jackfruit methanol extract showed the presence of 9-Octadecenoic acid (Z)-, 2-hydroxy-1, Octadecanoic acid and Benzene, 1, 2-dimethoxy-4-(1-propenyl). All these findings implied the availability of various phytochemicals might be a source of antifungal agent for inhibition of pathogens and to improve the postharvest quality of fruits.

Characterization of the Phytochemicals and Antioxidant Properties of Extracts From Wrightia Tinctoria R.Br.

Characterization of the Phytochemicals and Antioxidant Properties of Extracts From Wrightia Tinctoria R.Br.

Formulation of phytosomes with sun protection factor using Agave americana ethanolic extract

Over-exposure to the sun’s ultraviolet rays is a major environmental risk factor for skin cancer. Shortcomings associated with the use of synthetic sunscreens can be overcome by the use of herbal based sunscreens. Plant phytochemicals have antioxidant activity and Sun Protection Factor (SPF) activity, however their hydrophilic nature limits their ability to penetrate into deeper areas of the skin thereby affecting their bioavailability. The aim of this study was to formulate phytosomes with antioxidant and SPF activity using ethanolic extracts of Agave americana americana and Agave americana marginata leaves. Soxhlet extraction was used to extract phytochemicals from dried leaves. The presence of phenols, flavonoids, and tannins were confirmed using various tests. The antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay while SPF activity was determined using UV spectroscopy. Phytosomes were formulated and characterized for size, Polydispersity Index (PDI) and zeta potential. A. americana americana had a higher content of flavonoids, phenols, and tannins which also resulted in better antioxidant activity (IC50) 4.77 μg/ml versus 5.66 μg/ml and SPF activity of 9.95 versus 5.84, when compared to A. americana marginata. The phytosome formulations using A. americana americana had SPF activity of 9.96 to 13.3 with 40 mg extract and 60 mg soy lecithin having the highest activity. This formulation had the smallest particle size of 91nm, PDI and zeta potential of 0.235 and -4.95, respectively. These results indicate the formulation of the extracts into phytosomes enhances their SPF activity.

Phytochemical Characterization and Anticancer Screening of Cladophora goensis and Cladophora glomerata Extracts

Background Cladophora goensis and Cladophora glomerata has been widely identified in the Indian Oceans. Marine algae have been identified as potential pharmacological agents useful in the treatment of diabetes, hypertension, infections, and cancers. Objectives The present study aimed to evaluate the phytochemicals in C. goensis and C. glomerata using gas chromatography-mass spectroscopy (GC-MS), and to study the molecular interactions with cancer-related proteins using molecular docking techniques. Materials and methods GC-MS analysis was done using electron impact ionization at 70eV and the data was evaluated using total ion count for compound identification and quantification. AutoDock 4.0 version was used for the molecular docking analysis. Results The methanolic extracts of algae when subjected to GC-MS analysis, 19 compounds from C. goensis and 11 phytocompounds from C. glomerata were identified. The significant molecular interactions of phytochemicals of C. goensis (6-nitro-3 H-quinazolin-4-one, Isoquinoline, 1,2,3,4-tetrahydro-7-methoxy-2-methyl-8-(phenyl methoxy) and 9-Decen-1-ol, pentafluropropionate) and C. glomerata (phytol, palmitic acid, and octadec-9-enoic acid) against human epidermal growth factor receptor (4WRG), poly (ADP-ribose) polymerase-1 (4UND), human estrogen receptor alpha ligand-binding domain (3ERT), human peroxisome proliferator-activated receptor alpha ligand-binding domain (3VI8), and human topoisomerase (1EJ9) have been demonstrated. Conclusion The phytochemicals of methanolic extracts of C. goensis and C. glomerata showed potential interactions with cancer-related proteins.

Proximate Composition, Phytochemical Analysis and Toxicity Assessment of Extracts of Caulerpa lentillifera using Autoclave- and Microwave-Assisted Extractions

Caulerpa lentilliferais a marine alga widely cultured and consumed in Asia and Oceania. Emerging green techniques are currently popular for phytochemical extraction. Therefore, this study aimed to investigate the proximate compositions, phytochemicals, and antioxidant properties of the C. lentilliferaextracts using autoclave-assisted and microwave-assisted extraction methods (A-CLE and M-CLE, respectively). The toxicity of extracts was tested against human normal colon cells and freshwater crustacean Daphnia magna. We found that crude lipid, ash and moisture contents in A-CLE were lower than those in M-CLE, while crude protein and crude carbohydrate were higher in A-CLE. Total flavonoid content in A-CLE (1.474 ± 0.046 mg QE g-1 extract) was higher than that in M-CLE (1.179 ± 0.054 mg QE g-1 extract). There was no difference in total phenolic contents, triterpenoid contents and antioxidant activities between the extracts. The IC50 of A-CLE (12,260 ± 197 µg mL-1) was higher than that of M-CLE (10,950 ± 169 µg mL-1). In the acute toxicity test, the LC50 of A-CLE against D. magna (7.50 ± 0.28 g L-1) was lower than LC50 of M-CLE (8.76 ± 0.26 g L-1). This study suggest that autoclave-assisted and microwave-assisted extractions are effective green methods of extracting C. lentillifera. This study will be useful for further studies of C. lentillifera extracts to improve human and animal health.

Exploring Diverse Techniques for Phytochemical Extraction from Plant Sources A Comprehensive Review

Exploring Diverse Techniques for Phytochemical Extraction from Plant Sources A Comprehensive Review

Cytotoxic and Antitumoral Effects of Methanolic Extracts of Avocado Fruit Mesocarp in Colorectal Cancer Cell Line HT29.

Colorectal cancer is a widespread neoplasia with high ratios of chemoresistance. Phytochemicals in plant-based extracts could be useful to treat colorectal cancer, and/or reduce chemoresistance. Methanolic extract of avocado mesocarp (MEAM) has demonstrated antitumoral properties, depending on the fruit ripening stage (RS). The aim of this study was to analyze the effects of methanolic extracts of "Hass" avocado fruit at different RS on cytotoxicity, antioxidative, anti-inflammatory, anti-invasive, cell cycle, and epithelial-mesenchymal transition inhibition in colorectal adenocarcinoma cell line HT29. The MEAM showed an increasing concentration of total phenolic compounds as the RS progressed, which was correlated with antioxidant capacity measured by the Ferric Reducing Antioxidant Power assay but not with the 2.2-diphenyl-1-picrylhydrazyl assay. The specific phenolic compounds of MEAM were determined by high-performance liquid chromatography, and it was found that concentrations of epicatechin decreased while concentrations of chlorogenic acid increased as the RS progressed. The HT29 cell line was treated with MEAM for 48 h, and all MEAM had a cytotoxic effect, reported by MTT assay, nevertheless, the strongest effect was associated with the presence of chlorogenic acid. MEAM induced apoptosis and cell cycle arrest in phase G0/G1, reported by flow cytometry. Moreover, MEAM inhibited cell migration evidenced by the wound healing assay. On the other hand, MEAM significantly reduced expression of mRNA of tumor necrosis factor-alpha and cyclooxygenase 2. These effects comprise important inhibition of some hallmarks of cancer. This, in turn, may provide interesting guidelines for developing antitumoral intervention agents.