Phytoavailability Of Heavy Metals Research Articles

Phytoextraction of nickel, lead, and chromium from contaminated soil using sunflower, marigold, and spinach: comparison of efficiency and fractionation study.

Heavy metals in soil pose a serious threat through their toxic effect on the human food chain. Phytoremediation is a clean and green potentially cost-effective technology in remediating the heavy metal-contaminated soil. However, the efficiency of phytoextraction is very often limited by low phytoavailability of heavy metals in soil, slow growth, and small biomass production of hyper-accumulator plants. To solve these issues, accumulator plant(s) with high biomass production and amendment(s) which can solubilize metals in soil is required for better phytoextraction. A pot experiment was conducted to assess the efficiency of phytoextraction of sunflower, marigold, and spinach as affected by the incorporation of Sesbania (solubilizer) and addition of gypsum (solubilizer) in nickel (Ni)-, lead (Pb)-, and chromium (Cr)-contaminated soil. A fractionation study was conducted to study the bioavailability of the heavy metals in contaminated soil after growing the accumulator plants and as affected by using soil amendments (Sesbania and gypsum). Results showed that marigold was the most efficient among the three accumulator plants in phytoextraction of the heavy metals in the contaminated soil. Both sunflower and marigold were able to reduce the bioavailability of the heavy metals in the post-harvest soil, which was reflected in their (heavy metals) lower concentration in subsequently grown paddy crop (straw). The fractionation study revealed that carbonate and organically bound fractions of the heavy metals control the bioavailability of the heavy metals in the experimental soil. Both Sesbania and gypsum were not effective in solubilizing the heavy metals in the experimental soil. Therefore, the possibility of using Sesbania and gypsum for solubilizing heavy metals in contaminated soil is ruled out.

Biochar Is Not Durable for Remediation of Heavy Metal-Contaminated Soils Affected by Acid-Mine Drainage

Biochar is a soil conditioner for enhancing plant growth and reducing plants’ uptake of heavy metals. However, the protonation of biochar surfaces in acid soils can weaken the capacity of biochar to reduce the phytoavailability of soil-borne heavy metals over time. The aim of this study was to test this hypothesis by performing a plant-growth experiment with five harvest cycles to examine the durability of rice-straw biochar for the remediation of an acidic-mine-water-contaminated soil. The application of the biochar significantly reduced the phytoavailability of the heavy metals and inhibited the plant uptake of cationic heavy metals but not anionic Cr. The beneficial effects of the biochar were weakened with the increasing number of harvest cycles caused by the gradual protonation of the biochar surfaces, which resulted in the desorption of the adsorbed heavy metals. The weakening capacity of the biochar to reduce the heavy-metal uptake by the vegetable plants was more evident for Cu, Zn, and Pb compared to Ni and Cd. The experimental results generally confirmed the hypothesis. It was also observed that the bioaccessible amount of various metals in the edible portion of the vegetable was also reduced as a result of the biochar application.

Effects of solid waste-based soil conditioner and arbuscular mycorrhizal fungi on crop productivity and heavy metal distribution in foxtail millet (Setaria italica)

Shanxi is a large coal-producing province, and it also produces a lot of solid waste. Solid waste can leach heavy metals, which can harm soil and affect food security at the beginning of the food chain. To investigate the impacts of solid waste-based soil conditioner (SWSC) and arbuscular mycorrhizal fungi (AMF) on millet safety and crop production, a field experiment with foxtail millet (Setaria italica) was conducted in Tunliu. The results of this study demonstrate that SWSC + AMF, SWSC and AMF can increase millet yield by 28.0%, 27.1% and 19.5%, respectively, compared with CK. This is mainly due to increased mycorrhizal infection. Besides, the pollution index (Pi) and the Nemerow-integrated pollution index (PN) of the soil with SWSC and AMF were both below 0.7, indicating safe pollution levels. The application of AMF and SWSC inhibits plants from absorbing heavy metals from the soil and reduces the TFroot/soil of the millet. SWSC + AMF application inhibited the transfer of heavy metals from the roots to the upper part of the ground and reduced the TFshoot/root of the millet. The TFgrain/soil of the millet was below 1. The HQ and HI of the millet grains did not exceed 1, indicating the absence of a potential health risk. Therefore, SWSC combined with AMF is applicable for millet production in Tunliu, and the combined treatment can decrease heavy metal phytoavailability and post-harvest transfer risks. This work provides a way to utilize solid waste while also improving millet yields in dry farming. Based on the review, we suggested future researches to better understand the mechanisms of SWSC + AMF long-term application to promote awareness on its role over time through alterations in its surface chemistry, soil microbial community and environmental implications.

A comparison between the function of Serendipita indica and Sinorhizobium meliloti in modulating the toxicity of zinc oxide nanoparticles in alfalfa (Medicago sativa L.).

Zinc oxide nanoparticles (ZnO-NPs) are among the most commonly used nano-fertilizers (NF). However, elevated levels of ZnO-NPs in soil may affect plant growth and development due to its potential toxicity when accumulated in large amounts in plant tissues. This research was conducted using an in situ rhizobox system with the aims of evaluating zinc uptake from nano-zinc oxide amended rhizosphere soil by alfalfa plant and the effect of plant growth-promoting microorganisms on alleviating the phytotoxicity of ZnO-NPs. Treatments included microbial inoculations (Sinorhizobium meliloti, Serendipita indica) and different ZnO-NP concentrations (0, 400, and 800 mg kg-1) with three replications. The results indicated that S. indica minimized the phytotoxicity of ZnO-NPs to alfalfa by enhancing growth rate and decreasing zinc (Zn) translocation from root to shoot. Compared with plants inoculated with S. meliloti, co-inoculation with S. indica increased the shoot dry weight by 18.33% and 8.05% at 400 and 800 mg kg-1 ZnO-NPs, respectively. However, at the highest level of ZnO-NPs (800 mg kg-1), root inoculation of S. indica and S. indica + S. meliloti decreased Zn translocation factor by 60.2% and 44.3% compared to S. meliloti, respectively. Furthermore, a distinct relation between tolerance of S. indica-colonized plant to ZnO-NPs and the ability of S. indica in inhibiting or retarding degradation of polyunsaturated lipids through prevention of excess reactive oxygen species formation was observed. Malondialdehyde content of inoculated plants with S. indica either alone or in combination with S. meliloti was significantly lower than non-inoculated plants (p< 0.01). Zn-induced oxidative stress was mitigated by S. indica through enhanced activities of catalase and peroxidase enzymes. The findings of the present study indicate the potential use of endophytes fungus S. indica for ensuring food safety and security, and human health in heavy metal-polluted soil by reducing the phytoavailability of heavy metals in the aerial parts of the host plants.

Variations in phytoremediation potential and phytoavailability of heavy metals in different Salix genotypes subjected to seasonal flooding

Climate-related flooding poses a potential challenge to phytoremediation of metal polluted areas. In the present study, uptake, translocation and accumulation of heavy metals (Cd, Zn and Cu), and their phytoavailability in six flood-tolerant Salix genotypes were investigated under simulated seasonal flooding conditions (non-flooding conditions were kept as the control). Plants were cultivated in a greenhouse with open windows using the soil polluted with Cd, Zn and Cu for 110 days. All the control (non-flooded) genotypes did not exhibit visible toxic symptoms, whereas the flooded genotypes showed leaf chlorosis and developed both lenticels and adventitious roots. Biomass production and metal accumulation in tissues varied with Salix genotypes. The flooded genotypes dramatically decreased aerial biomass production compared with corresponding non-flooded genotypes. All the control Salix genotypes showed relatively high accumulation for Cd, Zn and Cu in aerial parts due to high EDTA-extractable metals in the rhizosphere, exhibiting phytoextraction features. In contrast, the flooded genotypes drastically decreased uptake, translocation, accumulation, and extraction capacities for Cd, Zn and Cu in aerial parts, differing with genotypes, and tended to phyto-stabilize them in roots, especially Cu. This study indicated that flooding is a leading factor on phytoremediation efficiency for contaminated sites with willows.

Effects of biochar derived from sewage sludge and sewage sludge/cotton stalks on the immobilization and phytoavailability of Pb, Cu, and Zn in sandy loam soil

Co-pyrolysis of sewage sludge and straws has been used to improve the pore structure and reduce the ecological risks of heavy metals in sewage sludge-derived biochars. However, to date, no study has focused on the effects of biochar derived from sewage sludge/straws on the immobilization and phytoavailability of heavy metals in soil. Here, we studied the effects of biochar derived from sewage sludge/cotton stalks (SCB) and that derived from sewage sludge alone (SSB) on the remediation of sandy loam soil contaminated by Pb, Cu, and Zn. SCB amendment decreased the bioavailable forms of Pb, Cu, and Zn in the soil by 19.0%, 34.9%, and 18.2%, respectively, and reduced their accumulation in ryegrass by 28.6%, 50.1%, and 30.0%, respectively, compared with those by SSB amendment. Furthermore, SCB amendment transformed more metals from the acid-soluble fraction to the oxidizable fraction than SSB amendment, indicating that complexation played a more critical role in SCB amendment than in SSB amendment. Both biochar amendments effectively improved soil water holding capacity, increased the supply of available P, N, and K, and promoted ryegrass growth. The findings of this study show the benefits of SCB over SSB for the remediation of heavy metal-contaminated soil.

Effect of some additives on heavy metals behavior and phytoavailability in municipal solid waste compost-amended soil

The effect of application of municipal solid waste compost (MSWC) on copper (Cu), lead (Pb) and cadmium (Cd) levels in soil and wheat plant was studied. The effectiveness of complementary remediations including compost washing (three levels), wood ash and sawdust application (each at three levels) was also examined. A completely randomized design pot experiment with medium-textured soil was performed. The diethylenetriaminepentaacetic acid–triethanolamine (DTPA–TEA) extracts of the soil and plant shoots were tested for heavy metal content using atomic absorption. The heavy metal contents of plant showed a definite dependence on extractable heavy metals in differently treated soil. The highest metal uptake by plant was observed in unleached compost-amended soil, followed by leaching, sawdust and wood ash remediations. No significant differences were observed within different levels of leaching and wood ash applications. The highest crop yield was obtained for wood ash amendment (1 wt%). Consequently, wood ash is an appropriate remediation for MSWC-treated soil to reduce heavy metal phytoavailability although monitoring of total heavy metal in soil is necessary for long-term application of MSWC.

Phytoavailability and human risk assessment of heavy metals in soils and food crops around Sutlej river, India

We assessed the phytoavailability of heavy metals in wheat and rice grown on soils along flood plains of Sutlej river (India) and its effects on human health. Surface soil samples along with wheat (pre-monsoon season) and rice (post-monsoon season) grain samples were collected during the years 2017 and 2018. Soil samples were analysed for total and DTPA-extractable metals and, wheat and rice grains samples for total metals. There were no significant (p < 0.05) changes in total metal concentration during both the years, but the concentration of DTPA-extractable metals was higher during pre-monsoon than post-monsoon season. The concentration of Pb, Cd, and Co in wheat grains, whereas the concentration of Cr, Co, Pb and Cd in rice grains surpassed their safe limits. Both DTPA-extractable metals (water soluble plus exchangeable) and non-exchangeable fractions explained more than 80% of the variations in metal uptake by plants. The bioaccumulation factor for DTPA-extractable metals (BCF) was above one, but Pollution Load Index for these metals (PLI) was less than one. The carcinogenic risks due to ingestion of wheat and rice grains were higher for Cd. The BAF, PLI and health risks were higher along transboundary of the river. These results suggest that exchangeable and non-exchangeable fractions are contributing towards metals uptake by wheat and rice along flood plain soils of the Sutlej river and the present study may act as a model for carrying out similar studies to find out the risks of heavy metals and it effect on human health in future.

Phytoavailability-based threshold values for cadmium in soil for safer crop production

The phytoavailability of heavy metals in soils is important for both food safety and environmental management. Hence soil metal phytoavailability threshold values need to be established based on a firm scientific basis. In this study, optimal Cd phytoavailability threshold values, were determined for bean, rice and sesame cultivated in 100 soils varying widely in soil chemical characteristics by comparing the soil Cd phytoavailability obtained using three commonly used extraction procedures. Subsequently, the transfer functions derived in this study, were used to establish soil Cd phytoavailability threshold value standard limits for each specific crop. In addition, independent experimental data were used to supplement the obtained soil phytoavailable Cd threshold value for rice. Soil phytoavailable Cd concentrations extracted by 1 M NH4NO3, 0.05 M EDTA and Mehlich3 solutions were each more significantly correlated with plant Cd concentrations than total soil Cd concentrations. Thus, the soil Cd phytoavailability threshold values proposed in this study provide a more effective means of ensuring safer agricultural food production. Therefore, it is recommended that current agricultural soil heavy metal management policy; which is based on total concentrations; should be changed to embrace soil metal phytoavailability for safer agricultural food production.

Particle size and rate of biochar affected the phytoavailability of Cd and Pb by mustard plants grown in contaminated soils

Various amendments are used to reduce the phytoavailability of heavy metals in contaminated soils, but recently the use of biochar is receiving serious attention. In this study, two particle sizes of an oil palm empty fruit bunch biochar (EFBB); <50 µm (F-EFBB) and >2 mm (C-EFBB) were applied at either 0, 0.5, or 1% (w/w) to soils contaminated with either Cd or Pb and the phytoavailability of these metals by mustard plants grown on the soils was evaluated. Results revealed that the application of EFBB at 1% significantly increased plant growth parameters as compared with the control in Cd-soil. However, there was no significant effect of EFBB application rate on plant growth parameters in Pb-soil. There was a significant difference in the concentrations of Cd and Pb in the plant root and shoot between soils receiving different particle sizes of EFBB. The treatment of 1% F-EFBB gave the lowest concentration of the Cd concentration in the shoot (115.200 mgkg−1) and Pb concentration in the root and shoot (4196.000 and 78.467 mgkg−1, respectively) as compared with the other treatments. Therefore, F-EFBB application at high rates can be recommended for reducing the phytoavailability of Cd and Pb in contaminated soils.

Heavy metal phytoavailability in a contaminated soil of northeastern Oklahoma as affected by biochar amendment.

High concentrations of heavy metals (HM) in soils have negative impacts on plants, human health, and the environmental quality. The purpose of this study was to evaluate the effects of biochars on the bioaccessibility of Zn, Pb, and Cd in a contaminated soil in the Tar Creek area of NE Oklahoma, as well as on the growth and uptake of these elements by perennial ryegrass (Lolium perenne). Biochars were produced from switchgrass (SGB) and poultry litter (PLB) feedstocks at 700 °C and applied to the soil at 0.0, 0.5, 1.0, 2.0, and 4.0% (w/w), with three replications. Regardless of the feedstock, both soil organic carbon (SOC or OC) and pH increased as the rates of biochars increased, which significantly decreased the HM bioaccessibility (p < 0.01). The Zn and Cd extracted by DTPA were highly correlated (p < 0.0001) with their concentration in ryegrass shoots and roots. Except for some significant positive correlations (p < 0.05), HM concentrations in ryegrass shoots and roots were not correlated with their biomass (p > 0.05). Both bioconcentration factor (BCF) and transfer factor decreased as the rates of biochars applied increased, especially for Pb and Cd (p < 0.01). Our results suggest it is beneficial to use biochars at Tar Creek as a soil amendment to reduce HM bioaccessibility and metal uptake by ryegrass.

Linking phytoavailability of heavy metals with microbial community dynamics during municipal sludge composting

Municipal sludge compost contains residual heavy metals (HMs), which could limit the utilization of compost as a resource. Studies have revealed that the availabilities of HMs in composting system are affected by microbial-driven organic matter and physicochemical indices. However, how the microbial community dynamics affect the availability of HMs during municipal sludge composting remains unclear. In this study, high-throughput sequencing technology was applied to investigate the response relationship between microbial dynamics and different availability of HMs. A total of 31 main genera within 5 phyla were identified. The concentrations of the most available HMs increased, and the increasing rates varied from 102% to 240%. Proteobacteria, Firmicutes, and Chloroflexi could affect organic matter to control the phytoavailability of Cr and Pb, whereas Proteobacteria, Bacteroidetes, and Actinobacteria could affect the phytoavailability of Cu and Zn. The bioaccessible HMs are only affected by organic functional groups, whereas the leachable HMs are not affected by organic matter or microorganisms. The “physicochemical index-microorganisms-available heavy metal” response pathway and principal component analysis showed that the increase in total C and N and the decreasing content of NO3−–N reduce the phytoavailable HMs. We finally proposed a regulation method to improve the resource utilization of municipal sludge composting and provide theoretical support for the harmlessness of municipal sludge composting.

Review of Heavy Metals Pollution in China in Agricultural and Urban Soils.

Background.The concentrations of heavy metals in soil and potential risks to the environment and public health are receiving increased attention in China.Objectives.The objective of this paper is to review and analyze heavy metals soil contamination in urban and agricultural areas and on a national scale in China.Methods.Initially, data on soil heavy metals concentration levels were gathered from previous studies and narratively analyzed. A further statistical analysis was performed using the geo-accumulation index (Igeo), Nemerow integrated pollution index (NIPI), mean, standard deviation (SD), skewness and kurtosis. Pollution levels were calculated and tabulated to illustrate overall spatial variations. In addition, pollution sources, remedial measures and impact of soil contamination as well as limitations are addressed.Results.The concentration level of heavy metals was above the natural background level in most areas of China. The problem was more prevalent in urban soils than agricultural soils. At the national level, the soil in most of the southern provinces and Beijing were heavily polluted. Even though the pollution condition based on Igeo was promising, the Nemerow integrated pollution level was the most worrisome. The soils in about 53% of the provinces were moderately to heavily polluted (NIPI>2). The effects were noticed in terms of both public and ecological health risks. The major sources were waste gas, wastewater, and hazardous residuals from factories and agricultural inputs such as pesticides. Efforts have been made to reduce the concentrations and health risks of heavy metals, including policy interventions, controlling contamination sources, reducing the phytoavailability of heavy metals, selecting and rearing of grain cultivars with low risk of contamination, paddy water and fertilizer management, land use changes, phytoremediation and engineering techniques.Conclusions. China is experiencing rapid economic and technological advancements. This increases the risk of heavy metals contamination of soil. If serious attention is not paid to this problem, soil toxicity and biological accumulation will continue to threaten the sustainability of China's development.Competing Interests.The authors declare no competing financial interests

Potato absorption and phytoavailability of Cd, Ni, Cu, Zn and Pb in sierozem soils amended with municipal sludge compost

Effects of sludge utilization on the mobility and phytoavailability of heavy metals in soil-plant systems have attracted broad attention in recent years. In this study, we analyzed the effects of municipal sludge compost (MSC) on the solubility and plant uptake of Cd, Ni, Cu, Zn and Pb in a soil-potato system to explore the mobility, potato plant uptake and enrichment of these five heavy metals in sierozem soils amended with MSC through a potato cultivation trial in Lanzhou University of China in 2014. Ridge regression analysis was conducted to investigate the phytoavailability of heavy metals in amended soils. Furthermore, CaCl2, CH3COONH4, CH3COOH, diethylene triamine pentacetic acid (DTPA) and ethylene diamine tetraacetic acid (EDTA) were used to extract the labile fraction of heavy metals from the amended soils. The results show that the MSC could not only improve the fertility but also increase the dissolved organic carbon (DOC) content of sierozem soils. The total concentrations and labile fraction proportions of heavy metals increase with increasing MSC percentage in sierozem soils. In amended soils, Cd has the highest solubility and mobility while Ni has the lowest solubility and mobility among the five heavy metals. The MSC increases the concentrations of heavy metals in the root, stem, peel and tuber of the potato plant, with the concentrations being much higher in the stem and root than in the peel and tuber. Among the five heavy metals, the bioconcentration factor value of Cd is the highest, while that of Ni is the lowest. The complexing agent (DTPA and EDTA) extractable fractions of heavy metals are the highest in terms of phytoavailability. Soil properties (including organic matter, pH and DOC) have important impacts on the phytoavailability of heavy metals. Our results suggest that in soil-potato systems, although the MSC may improve soil fertility, it can also increase the risk of soils exposed to heavy metals.

Multisurface modeling of Ni bioavailability to wheat (Triticum aestivum L.) in various soils

Continual efforts have been made to determine a simple and universal method of estimating heavy metal phytoavailability in terrestrial systems. In the present study, a mechanism-based multi-surface model (MSM) was developed to predict the partition of Ni(II) in soil–solution phases and its bioaccumulation in wheat (Triticum aestivum L.) in 19 Chinese soils with a wide range of soil properties. MSM successfully predicted the Ni(II) dissolution in 0.01 M CaCl2 extracting solution (R2 = 0.875). The two-site model for clay fraction improved the prediction, particularly for alkaline soils, because of the additional consideration of edge sites. More crucially, the calculated dissolved Ni(II) was highly correlated with the metal accumulation in wheat (R2 = 0.820 for roots and 0.817 for shoots). The correlation coefficients for the MSM and various chemical extraction methods have the following order: soil pore water > MSM ≈ diffuse gradient technique (DGT) > soil total Ni > 0.43 M HNO3 > 0.01 M CaCl2. The results suggested that the dissolved Ni(II) calculated using MSM can serve as an effective indicator of the bioavailability of Ni(II) in various soils; hence, MSM can be used as an supplement for metal risk prediction and assessment besides chemical extraction techniques.

A DOC coagulant, gypsum treatment can simultaneously reduce As, Cd and Pb uptake by medicinal plants grown in contaminated soil

The efficiency of gypsum, as a dissolved organic carbon (DOC) coagulator, for the simultaneous immobilization of two heavy metals (Cd and Pb) and one metalloid (As) in agricultural soils near an abandoned mining site was examined. The agricultural soil was defined as long-term contaminated as As (1540mgkg−1), Cd (55mgkg−1) and Pb (1283mgkg−1) concentrations exceeded the Korean guideline values for As (25mgkg−1), Cd (4mgkg−1), and Pb (200mgkg−1). Gypsum was incorporated into the contaminated soil at 3% (w/w). In comparison two commonly using immobilizing agents (lime and compost), together with a mixture (lime+gypsum) were also included in the pot trial for the cultivation of two medical plants (A. gigas and A. macrocephala) and to evaluate the effectiveness of gypsum on As, Cd and Pb immobilization. The results showed that even though pH change-induced immobilizing agents such as lime were more effective than gypsum at immobilizing Cd and Pb, addition of gypsum also effectively reduced heavy metal phytoavailability as indicated by decreases in the concentration of Cd and Pb in medicinal plants. Furthermore, gypsum and gypsum+ lime were also most effective in reducing As concentrations in both plants studied. This was mainly attributed to significant decreases in soil DOC (48–64%) when gypsum and gypsum+lime were applied to the soil. Consequently, it was concluded that enhanced DOC coagulation with gypsum, could be considered as a promising technique for the immobilization of both metals (Cd and Pb) and metalloids (As) in agricultural soils.

Potential use of lime combined with additives on (im)mobilization and phytoavailability of heavy metals from Pb/Zn smelter contaminated soils

This explorative study was aimed to assess the efficiency of lime alone and in combined with additives to immobilize Pb, Cd, Cu and Zn in soil and reduce their phytoavailability for plant. A greenhouse pot experiment was performed by using low and heavily contaminated top soils viz. Tongguan contaminated (TG-C); Fengxian heavily contaminated (FX-HC) and Fengxian low contaminated (FX-LC). The contaminated soils were treated with lime (L) alone and in combined with Ca-bentonite (CB), Tobacco biochar (TB) and Zeolite (Z) at 1% and cultivated by Chinese cabbage (Brassica campestris L). Results revealed that all amendments (p< 0.05) significantly reduced the DTPA-extractable Pb 97.33, Cd 68.06 and Cu 91.11% with L+TB, L+CB, L+Z in FX-LC soil and Zn 87.12% respectively, with L+CB into TG-C soil. Consequently, the application of lime alone and in combined with additives were drastically decreased the dry biomass yield of Brassica campestris L. as compared with control. Thus, these feasible amendments potentially maximum reduced the uptake by plant shoots upto Pb 53.47 and Zn 67.93% with L+Z and L+TB in FX-LC soil, while Cd 68.58 and Cu 60.29% with L+TB, L+CB in TG-C soil but Cu uptake in plant shoot was observed 27.26% and 30.17% amended with L+TB and L+Z in FX-HC and FX-LC soils. On the other hand, these amendments were effectively reduced the potentially toxic metals (PTMs) in roots upto Pb77.77% L alone in FX-HC, Cd 96.76% with L+TB in TG-C, while, Cu 66.70 and Zn 60.18% with L+Z in FX-LC. Meanwhile, all amendments were responsible for increasing soil pH and CEC but decreased soils EC level. Based on this result, these feasible soil amendments were recommended for long term-study under field condition to see the response of another hyper accumulator crop.

Functions of organic matter in polluted soils: The effect of organic amendments on phytoavailability of heavy metals

Soil degradation and/or contamination have significant influence on the quality of food. In such cases immobilization of contaminants in soil may often be applied. In this context, organic matter (especially humic and fulvic acids) presents in soil is favourable component component. It is because of their high sorption capacity with respect to many contaminants, including heavy metals, which may result in their immobilisation and, consequently protection of food and groundwater against contamination. Anthropogenically transformed soils are often poor in organic matter (humic substances), thus their role as a natural barrier is decreased. Therefore, re-generation of humic substances through humification of organic matter from diverse sources added to soils may be the way to re-built the protective function of the soil barrier, and consequently to reduce environmental and/or health risks at the areas under anthropopresion.The usefulness of organic matter contained in brown coal, brown coal-derived preparations and farmyard manure was studied as a factor decreasing the uptake of heavy metals by plants and/or their migration within groundwater by immobilizing them in soil. A conceptual model was developed for soil as a protective barrier against heavy metals uptake by plants and/or migration towards groundwater. Organic amendments to reduce immobilisation and thus the bioavailability of heavy metals in soils were tested in field stone pots. The phytoavailability of tested heavy metals was the lowest when a brown-coal derived preparation was used as an organic amendment, as indicated by the lowest bio-accumulation index (BI) of: 0.24 for Cd (winter wheat grain), 0.021 for Pb (winter wheat straw), and 0.48 for Zn (spinach).

Proposal of new convenient extractant for assessing phytoavailability of heavy metals in contaminated sandy soil.

The aim of the study was to compare the usefulness of 1M HCl with aqua regia, EDTA, and CaCl2 for the extraction of phytoavailable forms of Cu, Ni, and Zn on coarse-textured soils contaminated with these metals. Two microplot experiments were used for the studies. Reed canary grass (Phalaris arundinacea), maize (Zea mays), willow (Salix viminalis), spartina (Spartina pectinata), and miscanthus (Miscanthus × giganteus) were used as test plants. They were grown on soil artificially spiked with Cu, Ni, and Zn. The experimental design included a control and three increasing doses of metals. Microplots (1m2×1m deep) were filled with sandy soil (clay-6%, pH 5.5, Corg-0.8%). Metals in the form of sulfates were dissolved in water and applied to the plot using a hand liquid sprayer. During the harvest, samples were collected from aboveground parts, roots, and the soil and then tested for their Cu, Zn, and Ni contents. The metal content of the soil was determined using four tested extractants. It was found that Cu and Ni were accumulated in roots in bigger amounts than Zn. The usefulness of the extractants was evaluated based on the correlation between the content of metals in the soil and the plant (n=32). This study demonstrated that 1M HCl, aqua regia, and EDTA were more efficient or equally useful for the assessment of the phytoavailability of Cu, Ni, and Zn as CaCl2. Due to the ease of performing determinations and their low cost, 1M HCl can be recommended to assess the excess of Cu, Ni, and Zn in the coarse-textured soils.

Remediation of soils contaminated with heavy metals with an emphasis on immobilization technology.

The major frequent contaminants in soil are heavy metals which may be responsible for detrimental health effects. The remediation of heavy metals in contaminated soils is considered as one of the most complicated tasks. Among different technologies, in situ immobilization of metals has received a great deal of attention and turned out to be a promising solution for soil remediation. In this review, remediation methods for removal of heavy metals in soil are explored with an emphasis on the in situ immobilization technique of metal(loid)s. Besides, the immobilization technique in contaminated soils is evaluated through the manipulation of the bioavailability of heavy metals using a range of soil amendment conditions. This technique is expected to efficiently alleviate the risk of groundwater contamination, plant uptake, and exposure to other living organisms. The efficacy of several amendments (e.g., red mud, biochar, phosphate rock) has been examined to emphasize the need for the simultaneous measurement of leaching and the phytoavailability of heavy metals. In addition, some amendments that are used in this technique are inexpensive and readily available in large quantities because they have been derived from bio-products or industrial by-products (e.g., biochar, red mud, and steel slag). Among different amendments, iron-rich compounds and biochars show high efficiency to remediate multi-metal contaminated soils. Thereupon, immobilization technique can be considered a preferable option as it is inexpensive and easily applicable to large quantities of contaminants derived from various sources.