Physiological State Research Articles

Pilot plant cultivation of microalga Dictyosphaerium chlorelloides with night illumination from LEDs sources

Illumination during the night with white LEDs increased the growth of the microalga Dictyosphaerium chlorelloides strain CCALA 330 on a thin-film platform unit (150 L volume, 12 m2 area) approximately 2.5× in comparison to the platform illuminated only by the Sun. The mean PAR intensity on the Sun-illuminated unit was 71 μmol m−2 s−1, on the Sun + LEDs unit 549 μmol m−2 s−1, the mean temperatures were 15 °C and 20.1 °C. On the Sun unit the algae grew to a maximum of 15 g L−1 dry weight in 42 days, with Sun + LEDs into 17.8 g L−1 during 24 days when the both units reached the stationary phase of the growth curve. Biomass production was 3.3 in the Sun and 8.54 g m−2 d−1 in the Sun + LED, i.e. 0.27 and 0.68 g L−1 d−1. In total, the mean of 37.5 and 58.2 kWh per night were consumed, so the total electricity consumptions for biomass production was 0.20 and 0.40 kWh g−1 DW during LED + Sun cycles 1 and 2, respectively. The production of the extracellular polysaccharides was practically the same for both platforms, and constant during time. A more substantial double increase was only after 30 days of cultivation in both platforms and reached 4 g L−1. The fluorescence measurements proved good physiological state of the cultures. The PAR was found as a main driver of the photosynthetic activity. The correlation of the growth and fluorescence parameters to the environmental conditions was much more profound in the Sun pilot plant, therefore the reliable set of monitored parameters should be defined according to the cultivation type, for both of them we propose OD680/OD720 ratio as a proxy of nutrient deficiency.

WITHDRAWN: Non-Contact Blood Pressure Monitoring from Radar Signals by a Temporal-Spatial Feature Fusion Network

Non-contact blood pressure (BP) monitoring offers a comfortable and uninterrupted means of BP assessment, free from the constraints of physical contact. This capability is particularly better for the routine surveillance of BP for individuals with hypertension, burn injuries, skin sensitivities, and other pertinent conditions. In this study, we proposed a Temporal-Spatial Feature Fusion Network (TSFN), a radar-based, non-contact approach for BP monitoring. The TSFN architecture combines Residual Networks (ResNet) for the meticulous extraction of spatial features from radar signals, Gated Recurrent Unit (GRU) for the discernment of temporal dynamics, and Multiple Head Attention (MHA) to selectively emphasize crucial information. These components collectively ensure precise BP detection from radar signals. To enhance the model's robustness, a Pseudo-Huber loss function was employed to refine the optimization process, providing a smoother gradient transition and improved stability. Evaluations demonstrated impressive accuracies, with mean errors of 0.24±6.78 mmHg for systolic blood pressure (SBP) and 0.25±5.13 mmHg for diastolic blood pressure (DBP). These outcomes meet the standards set by the British Hypertension Society (BHS) for grade ‘A’ benchmarks for SBP and DBP measurements. Notably, the TSFN model avoids the need for complex feature engineering, demonstrating its effectiveness in monitoring BP fluctuations across diverse physiological states at 2s intervals. This feature highlights its potential applicability in real-time monitoring systems, offering a promising solution for continuous, non-contact BP monitoring.

CLK2 Condensates Reorganize Nuclear Speckles and Induce Intron Retention.

Intron retention (IR) constitutes a less explored form of alternative splicing, wherein introns are retained within mature mRNA transcripts. This investigation demonstrates that the cell division cycle (CDC)-like kinase 2 (CLK2) undergoes liquid-liquid phase separation (LLPS) within nuclear speckles in response to heat shock (HS). The formation of CLK2 condensates depends on the intrinsically disordered region (IDR) located within the N-terminal amino acids 1-148. Phosphorylation at residue T343 sustains CLK2 kinase activity and promotes overall autophosphorylation, which inhibits the LLPS activity of the IDR. These CLK2 condensates initiate the reorganization of nuclear speckles, transforming them into larger, rounded structures. Moreover, these condensates facilitate the recruitment of splicing factors into these compartments, restricting their access to mRNA for intron splicing and promoting the IR. The retained introns lead to the sequestration of transcripts within the nucleus. These findings extend to the realm of glioma stem cells (GSCs), where a physiological state mirroring HS stress inhibits T343 autophosphorylation, thereby inducing the formation of CLK2 condensates and subsequent IR. Notably, expressing the CLK2 condensates hampers the maintenance of GSCs. In conclusion, this research unveils a mechanism by which IR is propelled by CLK2 condensates, shedding light on its role in coping with cellular stress.

Circulating Proteins Associated with Anti-IL6 Receptor Therapeutic Resistance in the Sera of Patients with Severe COVID-19.

Circulating proteomes provide a snapshot of the physiological state of a human organism responding to pathogenic challenges and drug interventions. The outcomes of patients with COVID-19 and acute respiratory distress syndrome triggered by the SARS-CoV2 virus remain uncertain. Tocilizumab is an anti-interleukin-6 treatment that exerts encouraging clinical activity by controlling the cytokine storm and improving respiratory distress in patients with COVID-19. We investigate the biological determinants of therapeutic outcomes after tocilizumab treatment. Overall, 28 patients hospitalized due to severe COVID-19 who were treated with tocilizumab intravenously were included in this study. Sera were collected before and after tocilizumab, and the patient's outcome was evaluated until day 30 post-tocilizumab infusion for favorable therapeutic response to tocilizumab and mortality. Hyperreaction monitoring measurements by liquid chromatography-mass spectrometry-based proteomic analysis with data-independent acquisition quantified 510 proteins and 7019 peptides in the serum of patients. Alterations in the serum proteome reflect COVID-19 outcomes in patients treated with tocilizumab. Our results suggested that circulating proteins associated with the most significant prognostic impact belonged to the complement system, platelet degranulation, acute-phase proteins, and the Fc-epsilon receptor signaling pathway. Among these, upregulation of the complement system by activation of the classical pathway was associated with poor response to tocilizumab, and upregulation of Fc-epsilon receptor signaling was associated with lower mortality.

Seasonal Patterns of Resource Use Within Natural Populations of Burying Beetles.

For organisms in temperate environments, seasonal variation in resource availability and weather conditions exert fluctuating selection pressures on survival and fitness, resulting in diverse adaptive responses. By manipulating resource availability on a local spatial scale, we studied seasonal patterns of resource use within natural populations of burying beetles Nicrophorus vespilloides in a Norfolk woodland. Burying beetles are necrophagous insects that breed on vertebrate carcasses. They are active in Europe between April and October, after which they burrow into the soil and overwinter. Using breeding and chemical analyses, we compared the fecundity and physiological state of beetles that differed in their seasonal resource use. We found seasonal variation in carrion use by wild burying beetles and correlated differences in their reproductive success and cuticular hydrocarbon profiles. Our results provide novel insight into the seasonal correlates of behaviour, physiology and life history in burying beetles.

Variability in odor hedonic perception: A challenge for neurosensory and behavioral research.

Odor hedonic perception (pleasant/unpleasant character) is considered to be the first and one of the most important dimensions in olfaction and is known to be highly variable and dependent on several factors related to the stimulus, individual characteristics, and context. Although numerous experimental studies have been published on this topic, there is no comprehensive general review on the variability in odor hedonic perception. Therefore, the aim of this article was to describe and detail all the factors involved in the variability in odor hedonic perception. Stimulus properties-related variability includes the concentration/intensity and the physicochemical properties. Individual-related variability includes sex, age, and physiological state characteristics, while context-related variability involves factors associated with the stimulation context, such as stimulation pathway, exposure frequency, verbal influences, cross-modal associations, hedonic contrast, and cultural background. Subsequently, a section is devoted to the brain processing of odor hedonicity, although its role in odor hedonic variability is largely unknown. Finally, the article discusses the relationship between the hedonic qualities of odors and the resulting behavioral responses in both animals and humans. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Research on spacecraft environment control and astronaut health evaluation based on Modelica

Abstract The environmental control system of a manned spacecraft plays a pivotal role in maintaining the internal environment and temperature control, and it constitutes a fundamental component of the spacecraft. In this study, a Modelica-based model for the environmental control and health assessment of astronauts was examined with the objective of analysing the impact of the internal environment of the spacecraft on human physiological changes. The model employs multi-domain simulation techniques, integrating thermodynamic and hydrodynamic models to describe airflow and temperature changes, while incorporating human physiology models to capture the body’s response to these environmental changes. In particular, thermodynamic models are employed to calculate the transfer and distribution of heat within the cabin air, while hydrodynamic models are utilised to simulate airflow and pressure changes, which subsequently influence the breathing patterns and thermoregulatory mechanisms of the astronauts. The human physiology models include simulations of the cardiovascular, respiratory, and thermoregulatory systems. The coupling of these models enables the accurate prediction of the physiological state of astronauts under various environmental conditions. By modifying the environmental control parameters within the model, including air flow rate, temperature set point, and humidity level, the impact of disparate environmental control strategies can be evaluated. This method has the potential to be employed in the prediction of the health status of astronauts during various flight phases.

Unexplained Recurrent Pregnancy Loss: Clinical Application of Immunophenotyping.

Recurrent pregnancy loss (RPL) is defined as the failure of two or more pregnancies and affects approximately 5% of couples, often without a clear cause. The etiologies of RPL include factors such as maternal age, endocrine dysfunction, uterine abnormalities, chromosomal abnormalities, thrombophilias, infections, and autoimmune disorders. However, these conditions account for only 50%-60% of RPL cases. Research has explored whether an altered immune system, compared to the physiological state, may be linked to RPL. This review aims to determine whether specific immunophenotypes are associated with unexplained Recurrent Pregnancy Loss (uRPL) and whether targeted therapies addressing specific immunophenotypic alterations can improve pregnancy outcomes. A literature review was conducted using Pubmed/Medline, Scopus, and Embase databases, analyzing data from 95 articles published between 2001 and 2023. The roles of various cells of the immune system (B lymphocytes, T lymphocytes, natural killer cells, macrophages) in different tissues (peripheral blood, menstrual blood) were specifically investigated in women with uRPL. Specific immunophenotypes have been demonstrated to be associated with this condition. However, there is a need to standardize immunophenotyping assays and conduct more trials to stratify RPL risk and improve potential therapeutic strategies.

Design of the VOILA-intervention study: A 12-week nutrition and resistance exercise intervention in metabolic or mobility compromised Dutch older adults and the response on immune-metabolic, gut and muscle health parameters

BackgroundExercise and nutrition interventions can slow ageing-induced decline in physiology. However, effects are heterogeneous and usually studied separately per outcome domain. In the VOILA study, we simultaneously study various health outcomes relevant for older adults and the inter-individual heterogeneity in response to a lifestyle intervention. MethodsVOILA is a 12-week lifestyle intervention in 3 groups of older adults (≥60 years), with compromised mobility (n=50), compromised metabolic health (n=50), or recovering from total knee replacement (TKR, n=70, of which 20 randomized to standard care only). The intervention includes high-intensity resistance exercise training thrice weekly, nutritional counselling, and nutritional supplements every morning and evening (including 20-25g whey protein and (evening only) 5.5g Biotis™ GOS). We measure immune-metabolic, gut health, muscle mass and physical functioning at baseline and after completion of the intervention/standard care. An additional reference group of healthy older adults (n=50) will undergo baseline measurements only. DiscussionImprovements in various physiological systems are expected, but with differences between groups/individuals. This study will provide insights into how the physiological state of older adults influences the extent of lifestyle-induced health improvements to create better tailored interventions to attenuate biological ageing and improve the health span of subgroups and individuals.

The construction of an inflammation classification model for HDPCs applied in guiding pulpotomy based on single-cell Raman spectroscopy

The immune defense and the repair function of the pulp tissue serve as the biological foundation of pulpotomy. The precise evaluation of the pulp inflammation extent and determining its reversibility are essential for the success of pulpotomy. The objective of this study was to classify the molecular-level of dental pulp cell physiology and inflammatory state based on the biochemical changes obtained by single-cell Raman spectroscopy. Firstly, we differentiated the growth of HDPCs (human dental pulp cells) under physiological states by employing Raman spectroscopy with multivariate statistical analysis. Raman spectroscopy reflected the biochemical changes at different growth phases, including the lag phase, log phase, and stationary phase. Secondly, we evaluated the optimal concentration and duration of Porphyromonas gingivalis lipopolysaccharide (P.g.LPS) stimulation to establish a six-level inflammation classification model of HDPCs. Thirdly, we performed label-free characterization of biological component changes in cells of different inflammation grades by Raman spectroscopy. As a result, the differences of peaks in the region 600–1800 cm−1 demonstrated the biochemical molecular alterations in the different inflammation grades of HDPCs. As inflammation progresses in steps, protein peaks increased first and then decreased, while lipid and nucleic acid peaks gradually decreased compared to unstimulated cells. However, when the inflammatory stimulation reached grade V, the changes in the biological properties were characterized by a recovery in protein and lipid content, and a decrease in nucleic acid content. We then established the diagnostic model using the Raman spectra of HDPCs in physiological and inflammatory states, which had a prediction accuracy of 100 % and 97.4 %, respectively. Finally, we determined the reversibility threshold of HDPCs at different grades of inflammation. We observed that the inflammation of grade I and II cells had potential reversibility and could be attempted to be retained. In conclusion, Raman spectroscopy combined with multivariate statistical analysis has potential possibility to effectively distinguish the degree of inflammation in the dental pulp, thus providing new tools and perspectives on pulpotomy in clinical practice.

Serological study of Crimean – Congo haemorrhagic fever in Sheep in Thi – Qar province

Crimean Congo hemorrhagic fever virus (CCHFV) causes a lethal tick – borne zoonotic disease with severe clinical manifestation in humans but does not produce symptomatic disease in wild or domestic animals. It is endemic in a large region of the word. One of the main indicators that the disease is endemic in a region is the presence of antibodies specific to CCHFV in animal populations. The factors contributing to differential outcomes of infection between species are not understood. Serological studies have shown sheep are important to the survival of CCHFV in nature. Nevertheless, need for more studies on this subject in Iraq. Blood samples were taken from 200 sheep of different sex, variable age and physiological status. Only 100 samples used for physiological testes while 186 used for Microbiological test (ELISA) test. The present study was appeared a high level about (144)78.53% of positive carrier sheep while negative samples (42)21.46% in Thi – Qar province which include different regions such as Al-eakihah , Al- rifai and Al- garma region which recorded about 92.85% , 90% and 90 % respectively comparative with another region of thi qar province that appear low level of infected such as Al-fadhliya , Al-tar and Al- manar which recorded about 75%, 50% and 60.71% respectively. The current study focused on and attempted to connect the different physiological states, sex and age with infected animals. The male sex appears ahigh level of infected about 83.92% comparative with female was appeared low level of infected by CCHF virus about 74.61%, while recorded high infected in non – pregnant about 75% comparative with pregnant about 73,80 . Also, the present study was showed ahigh level of infected in age from 5 to nine years about 82.4% comparative with another age ranges which appeared about 71.11, 78.50 %.

Effects of age on the haematological and biochemical parameters in Romanian buffalo

The aim of this study was to investigate the changes of hematic constants related to age in Romanian buffalo, in order to establish references values based on age of the animals. A number of 45 Romanian buffalo animals, clinically healthy, were divided into 3 groups (n=15/group) according to their age, as follows: Group T1 = un-weaned buffalo calves of 1 to 3 months of age, Group T2 = young buffalo heifers of 6 to 12 months, and Group T3 = buffalo adult cows of 3 to 5 years of age. Different hematological parameters and biochemical parameters were investigated in the three groups. Generally, a significant effect of age on both hematological and biochemical indices studied was observed (P<0.05). The concentration of total cholesterol and the concentration of inorganic phosphorous were not influenced by age of the animals (P>0.05). Significant positive correlations were found between total protein and age of the animals (P=0.0019), while significant negative correlations were observed between hemoglobin (P=0.0227), white blood cells count (P=0.0352), lymphocytes (P=0.0002), platelet (P=0.0022), total cholesterol (P=0.1201), calcium (P=0.0077) and age of the animals. The results of the current pilot study could prove useful in establishing a baseline and health alarm thresholds for hematic parameters in water-buffalo, based on age of the animals and physiological status.

Performance and Physiological Responses of Pre-Weaning Peranakan Etawa Goats Fed Milk Replacer with Maggot Flour

Pre-weaning growth is crucial for achieving high weaning weights, which are expected to positively correlate with the overall performance of does/ewes. Milk replacer, designed to meet the nutritional needs of pre-weaning kids, offers a solution to this challenge. This study aimed to analyze and evaluate the impact of incorporating maggot flour into milk replacer on the performance of pre-weaned Peranakan Etawa (PE) crossbreed goats. The parameters observed included milk replacer intake, nutrient consumption, body weight gain, weaning weight, rectal temperature, respiratory rate, and feed cost per gain. The study involved 10 two-week-old local pre-weaned PE goats, each with an average body weight of 5.33 ± 0.93 kg, divided into two treatment groups with five replicates each. The treatments included a commercial milk replacer (P0) and a milk replacer containing maggot flour (P1). Data were analyzed using an independent t-test at a 5% significance level. The results showed that daily body weight gain in pre-weaned PE goats fed milk replacer containing maggot flour did not differ significantly (P0.05) from those fed the commercial milk replacer. The physiological responses of the pre-weaned goats, including rectal temperature and respiratory rate, were similar between treatments and did not negatively affect their physiological status. The lowest feed cost per gain was observed in the group fed the maggot flour-based milk replacer (P1), indicating its potential economic advantage.

Assessment of Gut Microbiome Variations in the Mother and Twin Infant of Captive White-handed Gibbons (Hylobates lar) Reveals the Presence of Beneficial and Pathogenic Bacteria

The white-handed gibbon (Hylobates lar) is one of the three Hylobatidae species found in the Peninsular Malaysia region. Studies on primate gut microbiota have yet to be conducted in Malaysian Hylobatidae, but previous studies have covered Cercopithecidae. Knowledge of the gut microbiota of endangered captive Malaysian Hylobatidae primates is important for health assessment and conservation management. Thus, the main objective of this study was to determine the gut microbiota profile of captive white-handed gibbons using the metabarcode 16S rRNA gene. Two H. lar fecal samples collected from the mother and twin infant at Zoo Melaka were used for DNA extraction. Next-generation sequencing (NGS) of the 16S rRNA gene was performed. The NGS data were analyzed on the basis of amplicon sequence variation. The gut microbiota of H. lar was dominated by Firmicutes and Bacteroidota because of their important roles in fermentation and nutrient assimilation from plant-based food sources. Predominance of Prevotella in the gut microbiota of H. lar indicates that the captivity condition causes the gut microbiota of gibbons to resemble that of humans. Treponema, a pathogenic bacterium, was also detected in the mother and twin infant. Variation in the bacterial community profile between the mother and the infant indicated differences in dietary adaptations and physiological state. This study provides an overview of gibbon health levels through gut microbiome screening and can be used when considering Malaysian primate health welfare and captive management.

Effects of Astaxanthin on the Physiological State of Porcine Ovarian Granulose Cells Cultured In Vitro.

The physiological state of Granulosa cells (GCs) is intricately linked to the growth and development of oocytes. Oxidative stress has been found to cause damage to GCs in vitro. Astaxanthin (AST), a well-known natural ketone-type carotenoid, has demonstrated strong antioxidant properties. This study investigates the impact of astaxanthin supplementation on the physiological state of porcine ovarian granulosa cells cultured in vitro. Variations in morphology, apoptosis, reactive oxygen species (ROS) levels, and the expression of apoptosis and anti-oxidation-related genes in porcine GCs from different passages were observed. Significant morphological changes, increases in apoptosis, and decreases in antioxidant capacity resulting from passage were observed. Subsequently, treatment with 5 μmol/L astaxanthin significantly enhanced cell viability, proliferation, antioxidant capacity and mitochondrial function while also regulating the estradiol (E2) and progesterone (P4) levels. Additionally, the gene expression of antioxidation, E2, and P4 synthesis markers was assessed, revealing reduced apoptosis and ROS levels in porcine GCs. In conclusion, supplementation with 5 μmol/L astaxanthin in vitro effectively enhances the physiological condition of porcine GCs and optimizes the culture system for these cells in vitro. Optimizing the culture system of porcine GCs in vitro can simulate the function of granulosa cells in vivo and provide a theoretical reference for further promoting follicular development, which is beneficial to improving sow fertility in actual production.

The influence of melatonin on the functional state of the human body during desynchronosis

In the article the mechanisms of melatonin action in the human body, exploring its impact on circadian rhythms, sleep, the immune system, and overall physiological function were considered. A focal point is the use of melatonin as a pharmacological tool to address dyssynchrony, particularly in students facing new time zones or geographic latitude changes. The study aims to understand melatonin's potential influence on regulating biological rhythms and the general physiological state in such scenarios. It scrutinizes how melatonin affects circadian rhythms, sleep, activity, and overall physiological balance during disruptions in biorhythmic synchronization. Recent studies were analyzed, revealing promising prospects for melatonin in regulating desynchronization and maintaining human health amid altered biological rhythms. Findings indicated that students in the experimental group, who took melatonin, experienced enhanced academic performance, increased work capacity, and improved mood. That underscored melatonin's positive impact on the functional state of the human body, even in the presence of desynchronosis. A study involving 100 students identified changes in circadian rhythms contributing to seasonal depression, with daily melatonin tablets proving effective in alleviating desynchronization symptoms. The article furnishes vital scientific data and practical recommendations, catering to professionals in biomedicine, biology, and pharmacology, as well as individuals keen on understanding human health and biological rhythms.

Older Adult Education in the Context of Psychological Theories of Ageing

In gerontology, the essence of ageing is traditionally equated with decline and degradation, it is considered a purposeful process of loss of the body's adaptive capacity. Psychologists, in particular representatives of behaviorism, deny this position, instead they consider ageing to be an integral part of the general process of human development. Theories of ageing have progressed from focusing on individuals’ declines and losses in later phase of their life course to considering ageing as a continuous process of human development. Psychological theories of ageing are crucial to understanding older adults as learners ‒ their needs, abilities, motivation of learning and expectations from educational activity. They help us to develop comprehensive approaches to building an effective educational environment and improving the learning process of that age and social group aiming to adapt that environment to the needs of older adult learners. The article analyzes the main provisions of psychological theories of ageing, which substantiate the importance and even the necessity of involving older adults in educational activities, because firstly, mental activity contributes to the preservation and even development of a person’s intellectual capabilities until old age; secondly, in older people (compared to young people), changes in the ability to learn are insignificant and relate rather to the sphere of perception, attention, motivation, and the physiological state of the body; and thirdly, older people have a growing need for a sense of security, which has a significant impact on their quality of life and is largely realized through communication and learning activities. Based on the main tenets of psychological theories of ageing, in our research we consider older adults as a resource and human capital. We believe that the approach to the problem of ageing and old age from the resource perspective, which is based on the ideas of theories of activity and lifelong personality development, allows us to justify the need for education of older adults and is the basis of educational gerontology.

Based on Sportomics: Comparison of Physiological Status of Collegiate Sprinters in Different Pre-Competition Preparation Periods.

This study aimed to assess the impact of pre-competition training by comparing the differences of collegiate sprinters in physiological state between strengthening and tapering training period by sportomics and combining their sport performance. Fifteen collegiate sprinters were investigated or tested on their body composition, dietary habits, energy expenditure, sleep efficiency, heart rate and respiratory rate during training, blood (blood cells, biochemical and immune markers) and urine (urinalysis), gut microbiome distribution, microbiome and blood metabolites, and their functions during the strengthening (Group A) and tapering training period (Group B) prior to competing in the national competitions. We found that 26.67% of sprinters achieved personal bests (PB) after the competition. The limb skeletal muscle mass and lymphocyte ratio of male sprinters in Group B were lower than those in Group A, and the serum creatine kinase (CK) level was higher than Group A (p < 0.05). The levels of serum CK, interleukin-6 (IL-6), interleukin-1β (IL-1β), and urine-specific gravity (SG) of the two groups were higher than the upper limit of the reference value. The detection rates of urine white blood cell (WBC) and protein in Group B were higher than those in Group A. The gut microbiome health index (GMHI) of Group A was higher than that of Group B, and the microbial dysbiosis index was lower than that of Group B. The ratio of Firmicutes/Bacteroidota (F/B) in Group A was higher than that in Group B. There were 65 differential metabolites in the A/B group, and the enriched pathway was mainly the NF-kappa B signaling pathway (up); B/T cell receptor signaling pathway (up); Th1 and Th2 cell differentiation (up); phenylalanine metabolism (up); and growth hormone synthesis, secretion, and action (up). There were significant differences in blood metabolites between the A and B groups, with a total of 89 differential metabolites, and the enriched pathway was mainly the NF-kappa B signaling pathway (up), T cell receptor signaling pathway (up), Th1 and Th2 cell differentiation (up), and glycerophospholipid metabolism (down). In conclusion, the imbalance of the gut microbiome and inflammation and immune-related metabolites of collegiate sprinters during the pre-competition tapering training period may be the cause of their limited sports performance.

Dang-Gui-Bu-Xue decoction against diabetic nephropathy via modulating the carbonyl compounds metabolic profile and AGEs/RAGE pathway

BackgroundDang-Gui-Bu-Xue decoction (DBD) is a traditional Chinese medicine prescription clinically employed for diabetic nephropathy (DN). However, the components and pharmacological mechanisms of DBD against DN remain incompletely understood. PurposeTo clarify the beneficial effect of DBD on DN and to explore its nephroprotective effect's probable mechanism and the main components. MethodsA diabetic mice model was established by feeding a high-fat diet (HFD) and intraperitoneal injections of streptozotocin (STZ, 40 mg‧kg-1). Subsequently, the mice were maintained on a HFD and administered with DBD. The benefits of DBD against DN were comprehensively assessed by monitoring energy and water intake, blood glucose and lipids, renal functions and pathological status. The UPLC-MS/MS was measured to detect chemical constituents in DBD and absorbed components in DBD-treated plasma under physiological and pathological states. Network pharmacology was employed to forecast the probable pathways of DBD intervention in DN, with subsequent validation of these predictions through testing biochemical parameters, anti-glycation and ELISA assays, immunofluorescence, immunohistochemistry, and western blotting. Then, a chemical derivatization method paired with UPLC-MS/MS analysis was performed to detect the carbonyl compounds in renal tissue. Finally, the main components of DBD against DN were screened by anti-glycation and MTT assays. ResultsDBD regulated energy and water intakes, glucose and lipid metabolism disorders, renal dysfunction, glomerular filtration rate, renal interstitial glycogen accumulation and fibrosis in HFD/STZ-induced DN mice. A total of 129 distinct chemical constituents in DBD were characterized, of which 28 were detected in the DBD-treated plasma under a pathological state. The network pharmacological results suggested AGEs/RAGE and its downstream pathway may be a potential pathway for DBD intervention in DN. Further experiments confirmed that DBD reduced renal oxidative stress by modulating the AGEs/RAGE pathway. Moreover, 21 differential carbonyl compounds were detected between normal and DN mice, and DBD significantly modulated 16. Ultimately, seven components were screened out in DBD, which may be the main components of DBD regulating carbonyl compounds metabolic profile and AGEs/RAGE pathway. ConclusionOur findings suggested for the first time that DBD could regulate the carbonyl compounds metabolic profile and AGEs/RAGE signaling pathway to ameliorate DN.

Wearable Sensors for Healthcare of Industrial Workers: A Scoping Review

Background and Objectives: This scoping review evaluates the use of wearable sensor technologies for workplace safety and health monitoring in industrial settings. The aim is to synthesize evidence on the impact of these sensors and their application in high-risk environments. Materials and Methods: Following the PRISMA guidelines, a systematic search across four international electronic databases yielded 59 studies, of which 17 were included in the final review. The selection criteria involved studies that specifically utilized wearable sensors to monitor various health and environmental parameters relevant to industrial workers. Results: The analysis categorizes wearable technologies into five distinct groups based on their function: gas monitoring technologies, heart rate and physiological data collection, fatigue and activity monitoring, comprehensive environmental and physiological monitoring, and advanced sensing and data collection systems. These devices demonstrated substantial benefits in terms of early detection of health risks and enhancement of safety protocols. Conclusions: The review concludes that wearable sensor technologies significantly contribute to workplace safety by providing real-time, data-driven insights into environmental hazards and workers’ physiological status, thus supporting proactive health management practices in industrial settings. Further research is recommended to address the challenges of data privacy, sensor reliability, and cost-effective integration to maximize their potential in occupational health safety.