Physiological Delivery Research Articles

Fetus descent simulation with the active uterine contraction during the vaginal delivery: MRI-based evaluation and uncertainty quantification

Finite element models ranging from single to multiscale models have been widely used to gain valuable insights into the physiological delivery process and associated complication scenarios. However, the fetus descent simulation with the active uterine contraction is still challenging for validation and uncertainty quantification issues. The present study performed a fetus descent simulation using the active uterine contraction. Then, simulation outcomes were evaluated using theoretical and in vivo MRI childbirth data. Moreover, parameter uncertainty and propagation were also performed. A maternal pelvis model was developed. The active uterine contraction was modeled using a transversely isotropic Mooney–Rivlin material. Displacement trajectories were compared between simulation, theoretical and in vivo MRI childbirth data. Monte Carlo (M.C) and Polynomial Chaos Expansion (PCE) methods were applied to quantify uncertain parameters and their propagations. Obtained results showed that fetal descent behavior is consistent with the MRI-based observation as well as the theoretical trajectory (curve of Carus). The head downward vertical displacement ranges from 0 to approximately 47 mm. A reduction of 50% in uterine size was observed during the simulation. Three high-sensitive parameters ( C 1 , C 2 , Ca 0 ) were also identified. Our study suggested that the use of the active uterine contraction is essential for simulating vaginal delivery but the global parameter sensitivity, parameter uncertainty, and outcome evaluation should be carefully performed. As a perspective, the developed approach could be extrapolated for patient-specific modeling and associated delivery complication simulations to identify risks and potential therapeutic solutions.

Sodium ampicillin release from biocompatible hydrogel with enhanced antibacterial characteristics

The goal of this research is to develop an intelligent carrier that can control the release of medications in response to particular triggers. A novel carrier was created by combining different amounts of chitosan and polyvinylalcohal along with specific amount of cinnamon extract to enhance the antibacterial response of the prepared hydrogels. A non-hazardous crosslinker 3-Aminopropyl(diethoxy)methylsilane (3-APDEMS) was used to study its effect on the physical and chemical properties of the produced injectable hydrogels as well as the controlled drug release behaviour. The hydrogels swelling behaviour was assessed in a range of solvent environments and the results indicated that the swelling ratio decreased as the concentration of 3-APDEMS rose. The hydrogels showed the most swelling at acidic pH values and the least swelling at basic and neutral pH values. They are appropriate for use in injectable controlled medication delivery systems due to their unique pH-sensitive characteristic at pH 7.To evaluate its release profile in this particular setting, ampicillin sodium salt was effectively added to the best hydrogel formulation (which contained 100 μL cinnamon extract and 150 μL 3-APDEMS).The results showed that the release of ampicillin sodium (AMS) was regulated in PBS (7.4), reached up to 98.236 % release over 90 min.The utilisation of these hydrogels as a cutting-edge material for physiological pH-controlled injectable medication delivery and other biological applications is supported by these results.

ICTP concentration in cervical-vaginal fluid as a potential marker of membrane collagen degradation before labor.

Numerous physical and chemical processes lead to rupture of membranes. Within the fetal membranes there are numerous types of metalloproteinases, which cause collagen type I degradation. The C-terminal telopeptide of colagen type I (ICTP) is the breakdown product of type I collagen. The aim of the study was to determine whether ICTP is secreted into the vaginal-cervical fluid (VCF) in the case of physiological rupture of the membranes of the fetus before delivery. The study was conducted in March 2021 at the Department of Obstetrics and Perinatology of the Jagiellonian University in Cracow, Poland. Twenty-three cases were included in the study. During routine gynecological examination with the use of specula, VCF was collected twice in a volume of 50 µL. The obtained material was then subjected to enzyme immunoassay using the Human C-telopeptide of type I collagen (ICTP) ELISA Kit (Catalog Number. CSB-E10363h). The concentration of ICTP in the sample was calibrated. The concentration range that the device can detect was 25 ng /mL-800 ng/mL. The presence of ICTP in the VCF was confirmed. The minimum concentration was 43.72 ng/mL, the maximum was 762.59, in five cases the concentration was outside the maximum scale of the device. ICTP was confirmed in the VCF of pregnant women before physiological delivery. Further studies are required to accurately evaluate ICTP as a marker of the processes of collagen degradation in fetal membranes in the mechanism of physiological labor and premature rupture of the membranes.

Fenomena Pengalaman Pengasuh Anak Korban Kekerasan Fisik di Kota Banda Aceh: Studi Kualitatif

Background: Violence against children is a global phenomenon that poses significant challenges to the health care system because it has serious consequences for victims. Purpose: To examine the experiences of caregivers in Banda Aceh who provide assistance to children who have been physically abused. The event under consideration involves perception, adaption reaction, physiological delivery, supporting the child's adaptation process concerning interdependence, and assisting the child's adaptation process concerning self-concept, role, and function. Methods: This study used a qualitative, phenomenological approach to its investigation. The caregiver serves as the major informant for this study, providing care and meeting the needs of children who are physically abused. Key informants have traits including providing for the everyday needs of the children they live with, living at home with child victims of violence, and effectively communicating their experiences. Interviews and pertinent literature reviews were used to gather research data, which was subsequently analyzed using the Colaizzi. Results: The study's findings are as follows: 1) The caregiver is aware that the child's perception of physical violence is a result of their wrongdoings, 2) The principal caregiver observes the detrimental effects of physical abuse on the kid, 3) The violent child receives physical assistance from the primary caregiver. current physiological requirements by obtaining resources, 4) By offering moral support, the caregiver assists children who experience physical abuse in their self-concept. 5) Using techniques to enhance the child's role function, the caregiver supports children who have experienced physical abuse in this area, and 6) The caregiver seeks counseling support in order to assist children who are physically abused in the area of interdependence. Conclusion: caregivers are capable of improving perception, adaptation reactions, physiological delivery, supporting the child's adaptation process based on interdependence, and helping the child adapt to self-conceptions, roles, and functions.

Thermal transport exploration of ternary hybrid nanofluid flow in a non-Newtonian model with homogeneous-heterogeneous chemical reactions induced by vertical cylinder

Studying the combination of convection and chemical processes in blood flow can have significant applications like understanding physiological processes, drug delivery, biomedical devices, and cardiovascular diseases, and implications for various fields can lead to developing new treatments, devices, and models. This research paper investigates the combined effect of convection, heterogeneous-homogeneous chemical processes, and shear rate on the flow behavior of a ternary hybrid Carreau bio-nanofluid passing through a stenosed artery. The ternary hybrid Carreau bio-nanofluid consists of three different types of nanoparticles dispersed in a Carreau fluid model, miming the non-Newtonian behavior of blood. This assumed study generates a system of PDEs that are processed with similarity transformation and converted into ODEs. Furthermore, these ODEs are solved with bvp4c. The results show that the convection, heterogeneous-homogeneous chemical processes, and shear rate significantly impact the bio-nano fluid’s flow behavior and the stenosed artery’s heat transfer characteristics.

Investigations on physiochemical and biomedical properties of Aloe vera - Sterculia gum copolymeric dressings impregnated with antibiotic-anesthetic drugs to enhance wound healing

Recently, various innovative advancements have been made in carbohydrate research to design versatile materials for biomedical applications. The current research focuses on the development of copolymeric hydrogel wound dressings (HWD) using a combination of aloe vera (AV) - sterculia gum (SG) - poly (vinylsulfonic acid) (VSA)-based with the aim to enhancing their efficacy in drug delivery (DD) applications. These hydrogel dressings were encapsulated with levofloxacin and lidocaine to address both microbial infection and pain. Copolymers were characterized by FESEM, SEM, EDS, AFM, 13C NMR, FTIR, XRD, and TGA-DTG analysis. Hydrogel exhibited a fluid absorption capacity of 4.52 ± 0.12 g per gram of polymeric dressing in simulated wound conditions. The hydrogels displayed a sustained release of drugs, demonstrating a non-Fickian diffusion mechanism. Polymer dressings revealed antibacterial, mucoadhesive, antioxidant, biocompatible and non-cytotoxic properties. Additionally, HWD displayed permeability to O2 and water vapour, yet was impermeable to microbial penetration. Overall, the findings of physiological, biochemical and drug delivery properties demonstrated the suitability of materials for wound dressing applications.

Chemotherapeutics-Loaded Poly(Dopamine) Core-Shell Nanoparticles for Breast Cancer Treatment.

Chemophotothermal therapy is an emerging treatment of metastatic and drug-resistant cancer anomalies. Among various photothermal agents tested, poly(dopamine) provides an excellent biocompatible alternative that can be used to develop novel drug delivery carriers for cancer treatment. This study explores the synthesis of starch-encapsulated, poly(dopamine)-coated core-shell nanoparticles in a one-pot synthesis approach and by surfactant-free approach. The nanoparticles produced are embellished with polymeric stealth coatings and are tested for their physiologic stability, photothermal properties, and drug delivery in metastatic triple-negative breast cancer cell (TNBC) lines. Our results indicate that stealth polymer-coated nanoparticles exhibit superior colloidal stability under physiologic conditions, and are excellent photothermal agents, as determined by the increase in temperature of solution in the presence of nanoparticles, upon laser irradiation. The chemotherapeutic drug-loaded nanoparticles also showed concentration-dependent toxicities in TNBC and in a brain metastatic cell line. SIGNIFICANCE STATEMENT: This study develops, for the first time, biocompatible core-shell nanoparticles in a template-free approach that can serve as a drug delivery carrier and as photothermal agents for cancer treatment.

Assessment of the Antigen-Binding Capacity and Separation of Extracellular Vesicles Coated with Antigen-Specific Antibody Light Chains.

Many researchers are interested in the possibility of manipulating the targeting specificity of extracellular vesicles (EVs) for their use as physiological delivery vehicles for drugs and bioactive molecules. Our studies demonstrated the possibility of directing EVs toward the desired acceptor cell by coating them with antigen-specific antibody light chains. Here, we describe the methods for detection of the presence of antibody light chains on the EV surface, proving their ability to specifically bind the antigen and for separating the antigen-binding EV subpopulation.

The Role of Ultra-Rapid-Acting Insulin Analogs in Diabetes: An Expert Consensus.

Ultra-rapid-acting insulin analogs (URAA) are a further development and refinement of rapid-acting insulin analogs. Because of their adapted formulation, URAA provide an even faster pharmacokinetics and thus an accelerated onset of insulin action than conventional rapid-acting insulin analogs, allowing for a more physiologic delivery of exogenously applied insulin. Clinical trials have confirmed the superiority of URAA in controlling postprandial glucose excursions, with a safety profile that is comparable to the rapid-acting insulins. Consequently, many individuals with diabetes mellitus may benefit from URAA in terms of prandial glycemic control. Unfortunately, there are only few available recommendations from authoritative sources for use of URAA in clinical practice. Therefore, this expert consensus report aims to define populations of people with diabetes mellitus for whom URAA may be beneficial and to provide health care professionals with concrete, practical recommendations on how best to use URAA in this context.

Mood disorders in postpartum patients at the University Hospital in Zielona Góra according to the Edinburgh Postnatal Depression Scale (EPDS)

Introduction. Childbirth and its course have a significant impact on a woman’s mental functioning during the postpartum period and motherhood. The mother’s mental state affects not only her child’s life and health, but also the functioning of the entire family. The literature distinguishes several types of mental disorders that occur in the postpartum period, including postpartum depression. Aim. This study aims to determine the incidence of mood disorders in women up to 6 months postpartum. Material and methods. The research was carried out using a standardized questionnaire – the Edinburgh Postnatal Depression Scale (EPDS) – designed to determine the severity of mood disorders in the postpartum period in women at the University Hospital in Zielona Góra. The survey was conducted among 100 women aged from 17 to 43. Results. The results of the analysis showed that in women who noticed mood disorders during pregnancy (n=11, i.e. 11% of the total and n=10, i.e. 10% of the total), there is a possibility of postpartum depressive disorders (score ≥12 points on the ESDP scale) (p=0.0006). Respondents who answered “Definitely yes”, accounted for 42.31% of women who scored ≥12 points on the ESDP scale, and those who answered “Rather yes”, accounted for 38.46%. Conclusion. Respondents, whose pregnancy ended in a physiological delivery (forces of nature), feel more satisfied with childbirth than the other respondents (n=29 – definitely yes; n=24 – rather yes). In women whose pregnancy ended in caesarean section, the level of dissatisfaction with childbirth (n=9 – rather not) or less satisfaction (n=3 – definitely not) was higher than in the other women (p=0.0124). Keywords: depression, childbirth, depression scale

A Rapid-Patterning 3D Vessel-on-Chip for Imaging and Quantitatively Analyzing Cell-Cell Junction Phenotypes.

The blood-brain barrier (BBB) is a dynamic interface that regulates the molecular exchanges between the brain and peripheral blood. The permeability of the BBB is primarily regulated by the junction proteins on the brain endothelial cells. In vitro BBB models have shown great potential for the investigation of the mechanisms of physiological function, pathologies, and drug delivery in the brain. However, few studies have demonstrated the ability to monitor and evaluate the barrier integrity by quantitatively analyzing the junction presentation in 3D microvessels. This study aimed to fabricate a simple vessel-on-chip, which allows for a rigorous quantitative investigation of junction presentation in 3D microvessels. To this end, we developed a rapid protocol that creates 3D microvessels with polydimethylsiloxane and microneedles. We established a simple vessel-on-chip model lined with human iPSC-derived brain microvascular endothelial-like cells (iBMEC-like cells). The 3D image of the vessel structure can then be "unwrapped" and converted to 2D images for quantitative analysis of cell-cell junction phenotypes. Our findings revealed that 3D cylindrical structures altered the phenotype of tight junction proteins, along with the morphology of cells. Additionally, the cell-cell junction integrity in our 3D models was disrupted by the tumor necrosis factor α. This work presents a "quick and easy" 3D vessel-on-chip model and analysis pipeline, together allowing for the capability of screening and evaluating the cell-cell junction integrity of endothelial cells under various microenvironment conditions and treatments.

Husbands' Perception of Environmental Characteristics During Participation in Physiologic Delivery.

Nowadays, the presence of husbands during the childbirth process is regarded as an important factor that can contribute to lower rate of the C-section, better success of physiologic delivery and higher satisfaction in childbirth experience. Considering the special sociocultural characteristics of the Iranian society, this method requires accurate assessment to be practical and effective. The aim of this research was finding out how husbands perceive the environmental and physical characteristics of delivery spaces via studying these physical characteristics, exploring them through husbands' perception and finally discovering the mechanism behind formation of this perception. First, the conceptual model of the study was developed after a review of the physiologic childbirth literature. Then, a research questionnaire was designed and distributed among 120 husbands who recently had the experience of accompanying their wives during physiologic childbirth. The results showed that light, color and temperature play a significant role in husbands' perception of delivery spaces under the special psychological conditions and stress that they experience during the childbirth. Designer should pay due attention to these three factors when designing the structure of childbirth spaces. Promoting the environmental quality of the physiologic delivery room based on husband's perception can help mothers and medical staff better manage childbirth pain which is an integral part of natural childbirths.

Nitric Oxide: Physiological Functions, Delivery, and Biomedical Applications.

Nitric oxide (NO) is a gaseous molecule that has a central role in signaling pathways involved in numerous physiological processes (e.g., vasodilation, neurotransmission, inflammation, apoptosis, and tumor growth). Due to its gaseous form, NO has a short half-life, and its physiology role is concentration dependent, often restricting its function to a target site. Providing NO from an external source is beneficial in promoting cellular functions and treatment of different pathological conditions. Hence, the multifaceted role of NO in physiology and pathology has garnered massive interest in developing strategies to deliver exogenous NO for the treatment of various regenerative and biomedical complexities. NO-releasing platforms or donors capable of delivering NO in a controlled and sustained manner to target tissues or organs have advanced in the past few decades. This review article discusses in detail the generation of NO via the enzymatic functions of NO synthase as well as from NO donors and the multiple biological and pathological processes that NO modulates. The methods for incorporating of NO donors into diverse biomaterials including physical, chemical, or supramolecular techniques are summarized. Then, these NO-releasing platforms are highlighted in terms of advancing treatment strategies for various medical problems.

Fetal-maternal gas transport and acid – base balance in preterm labors

The use of cord blood gases to predict hypoxic stress during high-risk labor has been known since 1958. This diagnostic test is widely used, especially in specialized clinics, and blood indicators in the umbilical artery accurately reflect the condition of the newborn. However, there is a lack of specific diagnostic criteria for premature births.The objective: to study the ratio of indicators of gas exchange and acid-base status between the newborn and the mother depending on the gestational age at the time of delivery.Materials and methods. The levels of pH, pO2, pCO2, bicarbonate ion concentration (HCO3-) and base excess (BE) were analyzed both in mothers (maternal venous blood, v. cubitalis) and in their newborns (venous blood obtained from the umbilical artery).Study groups: I group – 16 postpartum women with newborns at 24–27 weeks of gestation, II group – 36 postpartum women with newborns at 28–34 weeks, III group (control group) – 24 postpartum women with newborns at term physiological delivery (37–41 weeks).Results. The study found no statistically significant differences in maternal venous and umbilical artery of pH, pO2, pCO2, HCO3- and BE levels between the term pregnancy group and the groups of preterm labor. However, extremely preterm neonates were found to have a marked increase in pH and BE in response to hyperventilation during labor.Conclusions. 1. Preterm infants had higher venous blood pH levels obtained from the umbilical artery, which were more pronounced in those who were born at earlier gestational ages (7.36±0.011 at 24–27 weeks gestation and 7.33±0.022 at a gestation period of 28–34 weeks), compared to full-term children (7.29±0.045; p<0.05).2. The difference in the pH levels of the venous blood of the newborn, obtained from the umbilical artery, and the mother’s blood, obtained from the v. cubitalis, is smaller in premature births (for a gestation period of 24–27 weeks – 7.39±0.018 in maternal blood and 7.36± 0.011 – in newborns; for the period of 28–34 weeks – 7.40±0.021 and 7.33±0.022, respectively) compared to the levels in full-term babies (7.43±0.015 – in maternal blood and 7.29±0.045 – in a newborn); p<0.05. This is primarily due to a decrease in the mother’s venous blood pH.

Cardiopulmonary bypass in neonates and infants: advantages of high flow high hematocrit bypass strategy-clinical practice review.

Cardiopulmonary bypass is an integral and indispensable part of surgical repair of congenital heart defects. While the complications and morbidity secondary to the use of cardiopulmonary bypass has decreased considerably, there remains a significant incidence of clinically relevant renal and neurological injury. To provide more physiological delivery of oxygenated blood to the end-organs, our center has been successfully using a high-flow, high hematocrit cardiopulmonary bypass strategy since 2006. The essential components of this strategy include maintaining high flows (typically 200 mL/kg/min in neonates, 150-175 mL/kg/min in older infants weighing <10 kg, and 2.6 L/min/m2 in older children) throughout the duration of cardiopulmonary bypass irrespective of patient temperature, as well as maintaining a hematocrit of at least 32% on cardiopulmonary bypass. The incidence of post-operative acute kidney injury (around 3%) and clinical acute neurological events (<1%) with this strategy is considerably less when compared to other contemporary publications using the conventional cardiopulmonary bypass strategy. In this review, we discuss the rationale behind our approach and present evidence to support the high-flow, high-hematocrit strategy. We also discuss the practical aspects of our strategy and describe the adjuncts we use to derive additional benefits. These adjuncts include the use of a hybrid pH/alpha stat strategy during cooling/rewarming, aggressive use of conventional ultrafiltration during cardiopulmonary bypass, a terminal hematocrit of 40-45%, and avoidance of milrinone and albumin in the early peri-operative period. This results in a very low incidence of post-operative bleeding, facilitates chest closure in the operating room even in most neonates, helps in reducing the need for post-operative blood product transfusion and helps in achieving a favorable post-operative fluid balance early after surgery.

Pemberdayaan Ibu Hamil Tentang Senam Hamil Melalui Pelayanan Home Care di Kabupaten Bulukumba

Pregnancy exercise is a movement therapy therapy given to pregnant women to prepare themselves both physically and mentally for childbirth. Pregnancy exercise is needed by every pregnant woman, because it can make the body fit and healthy, and able to carry out daily activities, so that stress due to anxiety before giving birth can be minimized. However, many pregnant women do not do pregnancy exercise because they are unsure and afraid of their pregnancy if they do pregnancy exercise. Pregnancy exercise exercises that are carried out regularly both at the gym and at home in their spare time can lead pregnant women towards a physiological delivery as long as there are no pathological conditions accompanying the pregnancy. The purpose of Community Service in the Work Area of the Caile Health Center is to increase the ability of pregnant women and cadres to practice pregnancy exercises, so that they will increase the knowledge and health of pregnant women and prepare for childbirth. This community service activity begins with training for existing Posyandu cadres, providing supporting facilities and infrastructure in the form of leaflets and modules for pregnant women exercise, and doing pregnancy exercises in home care. The results of this Community Service activity are the formation of pregnancy exercise cadres, pregnant women can practice pregnancy exercise at home, as well as the provision of facilities and infrastructure in the form of leaflets and pregnancy exercise modules

Could Electromyographic and Pressure Related Signals Identify Differences in Abdominal Activity and Postural Control between Women with and without C-Section?

Scars interfere with the motor system; however, the influence of c-section scars has not been described yet. The aim of this study is to relate the presence of abdominal scars from a caesarean section with changes in postural control-stability and orientation and abdominal and lumbar neuromuscular control in the orthostatic position. Cross-sectional analytical observational study comparing healthy primiparous women with caesarean delivery (n = 9) and physiologic delivery (n = 12) who have delivered more than one year before. The relative electromyographic activity of the rectus abdominis, transverse abdominis/oblique internus and lumbar multifidus muscles, antagonist co-activation, the ellipse area, amplitude, displacement, velocity, standard deviation, and spectral power of the centre of pressure, and thoracic and lumbar curvatures, were evaluated in the standing position in both groups, through an electromyographic system, a pressure platform and spinal mouse system. In the "caesarean delivery" group, scar mobility was evaluated using a modified adheremeter. Significant differences in CoP medial-lateral velocity and mean velocity were observed between groups (p < 0.050), while no significant differences were in the level of muscle activity, antagonist co-activation, and thoracic and lumbar curvatures (p > 0.05). The information provided by the pressure signal seems to identify postural impairments in women with c-sections.

Intervention of Kusuma Milk-Shake Drink on Cervical Dilatation and Duration of Labor: Experience from Bengkulu, Indonesia

Background: Labor processes require energy consumption regulated by a complex nervous system and hormonal response. Thus, the intensity of maternal nutritional intake since pregnancy should be highly considered for physiological delivery. Nutrients that are high in energy and provide fast-decomposing glucose intake should be chosen by the mothers. Kusuma is a kind of drink made from dates, milk, and honey. This study aimed to analyze the effect of Kusuma milk-shake drink implementations on cervical dilatation and duration of labor.Methods: An experimental study was conducted by post-test only with a controlled group design, including 34 mothers with term pregnancy, which was intervention groups and controls. A completely randomized design in two different places was conducted. Data were analyzed using Shapiro-wilk, independent sample T-test, and Mann-Whitney. Results: There was a significant effect of Kusuma milk-shake implementation on cervical dilatation (p=0.000). The dilatation process was found to be 5.5 times faster, and the duration of labor (p=0.000) was observed to be 9.7 hours faster than the control group.Conclusions: Implementation of the Kusuma milk-shake has a significant effect on the acceleration of cervical dilation and the duration of labor. This research is expected to improve the quality of midwifery services as an effort to realize the concept of a Continuum of Obstetric Care.

Hubungan Pendidikan dan Paritas dengan Keikutsertaan Ibu Hamil dalam Kelas Yoga di Desa Kalipang Kabupaten Kediri

Pregnancy is a natural method experienced by women and is not included in the pathological category except in special conditions, because pregnancy will experience both anatomical, physiological and biochemical adaptations which increase according to gestational age. During pregnancy there will be changes in all body systems both physically and psychologically One method that can be used to help and relieve pregnant women in preparing for a healthy pregnancy and physiological delivery is exercise. One good exercise for pregnant women is yoga for pregnant women / prenatal yoga. This research is a type of analytic quantitative research using a cross-sectional approach with the independent variables being Education and parity and the dependent variable being the participation of pregnant women in yoga classes with analysis using the Chi Square test. Total sampling data analysis. From the research data, the results of statistical analysis showed that there was no relationship between education and parity with the participation of pregnant women in yoga classes in Kalipang Village, Kediri Regency in 2023 with a P value of 0.463.

The advent of a pan-collagenous CLOVIS POINT for pathotropic targeting and cancer gene therapy, a retrospective.

The 'Clovis Point'-an enabling prehistoric gain-of-function in stone-age tool technologies which empowered the Paleoindian-Americans to hunt, to strike-deep, and to kill designated target megafauna more efficiently-was created biochemically by molecular-genetic bio-engineering. This Biomedical "Clovis Point" was crafted by adapting a broad-spectrum Pan-Collagen Binding Domain (Pan-Coll/CBD) found within the immature pre-pro-peptide segment of Von Willebrand Factor into a constructive series of advanced medical applications. Developed experimentally, preclinically, and clinically into a cutting-edge Biotechnology Platform, the Clovis Point is suitable for 1) solid-state binding of growth factors on collagenous scaffolds for improved orthopedic wound healing, 2) promoting regeneration of injured/diseased tissues; and 3) autologous stem cell capture, expansion, and gene-based therapies. Subsequent adaptations of the high-affinity Pan-Coll/CBD (exposed-collagen-seeking/surveillance function) for intravenous administration in humans, enabled the physiological delivery, aka Pathotropic Targeting to diseased tissues via the modified envelopes of gene vectors; enabling 4) precision tumor-targeting for cancer gene therapy and 5) adoptive/localized immunotherapies, demonstrating improved long-term survival value-thus pioneering a proximal and accessible cell cycle control point for cancer management-empowering modern medical oncologists to address persistent problems of chemotherapy resistance, recurrence, and occult progression of metastatic disease. Recent engineering adaptations have advanced the clinical utility to include the targeted delivery of small molecule APIs: including taxanes, mAbs, and RNA-based therapeutics.