Physio-biochemical Traits Research Articles

Correlation between Pulp Weight and Physio-Biochemical Traits of Mango (Mangifera indica L.) Germplasms

Aim: The present study was conducted to evaluate the relationship between pulp weight and various physio-biochemical attributes among 30 diverse mango (Mangifera indica L.) germplasms under the Tarai region of Uttarakhand. Place and Duration of Study: This investigation was carried out at Horticulture Research Centre, GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, during the years 2021 and 2022. Methodology: The selected 30 germplasms included two commercially grown cultivars and 28 primary germplasms sourced from different eco-geographical locations of Uttar Pradesh and Uttarakhand states of India. Pulp weight, physical characteristics (fruit shape index, pulp/stone ratio) and biochemical parameters (Brix/Acid ratio and pH) were measured for each germplasm and statistically laid out in completely randomized design (CRD), and their relationships were analyzed using simple linear regression and Pearson’s correlation coefficients. Results: The maximum values recorded among the studied germplasms were 300.73 g for pulp weight (PMGC-19), 2.41 for fruit shape index (PMSS-31), 0.309 for pulp/stone ratio (PMGC-71), 140.87 for Brix/Acid ratio (PMGC-96) and 5.87 for pH (PMGC-96). Among the physical attributes, pulp weight exhibited a moderate positive correlation with fruit shape index (r = 0.419), indicating that fruits with a higher shape index tend to have increased pulp weight. Whereas, a moderate negative correlation was observed between pulp weight and pulp/stone ratio (r = -0.457), suggesting that as the pulp/stone ratio increases, pulp weight decreases. However, biochemical attributes such as Brix/Acid ratio and pH showed no significant correlation with pulp weight (r = 0.005 and r = 0.018, respectively), indicating an independent influence of these parameters on pulp characteristics. Conclusion: These findings provide important insights into the complex relationships between pulp weight and other physio-biochemical traits in mango, some traits for breeding programs aimed to improving fruit quality and pulp content and identified superior and promising germplasms for cultivation as well as potential parental genotypes in mango improvement programs.

Physio-biochemical and molecular characterization of wheat cultivars (Triticum aestivum L.) under post-anthesis heat stress

BackgroundHeat stress is one of the abiotic stresses that make wheat crops vulnerable, which significantly impacts crop production around the world. An increase in temperature during the reproductive phase (anthesis) beyond the optimal range of 15–20 °C leads to decreased crop production, poor quality of the grain, and altered physiological and biochemical processes. To study the association between high temperature and physio-biochemical traits under normal and late sown, a set of fifteen genotypes was utilized.ResultsRelative water content under high temperatures had an overall decrease of 8.7%. However, grain protein and malondialdehyde content were higher in the stressed conditions than in the control, with increases of 20.2% and 38.9%, respectively. Marker Xgwm67, located on chromosome 5B, was found to be significantly associated with malondialdehyde content (R2 = 21%) and Xgwm570, located on 6A, was closely linked to relative water content as well as grain protein content (R2 = 16%) revealed by regression analysis. The correlation matrix displays a positive association between the control and stressed condition by R2 = 0.92, 0.82, and 0.53 in malondialdehyde, relative water content, and grain protein, respectively. However, there was a negative correlation between water content–malondialdehyde and malondialdehyde–grain protein, though there was only a 4% correlation between grain protein content (control) and relative water content (stressed). Based on the tolerance matrix, WH730 and RAJ4079 were heat tolerant, and DBW173 and HD3086 were sensitive.ConclusionsThese findings indicate that to identify tolerant genotypes, physiological and biochemical traits can be utilized as an alternate criterion, and these closely associated markers can be applied for improved late-planted wheat production through MAS. The breeding scheme and genome editing by recognizing novel genes through physio-biochemical parameters, marker-assisted selection, and prospective screening of tolerant genotypes are proclaimed by the study.

Comparative assessment of pantothenic, aspartic, ascorbic and tartaric acids assisted Pb-phytoextraction by sunflower (Helianthus annuus L.).

Phytoextraction of lead (Pb) is a challenging task due to its extremely low mobility within soil and plant systems. In this study, we tested the influence of some novel chelating agents for Pb-phytoextraction using sunflower. The Pb was applied at control (0.0278mM) and 4.826mM Pb as Pb(NO3)2 through soil-spiking. After 10days of Pb addition, four different organic ligands (aspartic, ascorbic, tartaric, and pantothenic acids) were added to the soil at 1mM concentration each. respectively. In the absence of any chelate, sunflower plants grown at 4.826mM Pb level accumulated Pb concentrations up to 104µgg-1 DW in roots, whereas 64µgg-1 DW in shoot. By contrast, tartaric acid promoted significantly Pb accumulation in roots (191µgg-1 DW; + 45.5%) and shoot (131.6µgg-1 DW; + 51.3%). Pantothenic acid also resulted in a significant Pb-uptake in the sunflower shoots (123µgg-1 DW; + 47.9%) and in roots (177.3µgg-1 DW; + 41.3%). The least effective amongst the chelates tested was aspartic acid, but it still contributed to + 40.1% more Pb accumulation in the sunflower root and shoots. In addition, plant growth, biochemical, and ionomic parameters were positively regulated by the organic chelates used. Especially, an increase in leaf Ca, P, and S was evident in Pb-stressed plants in response to chelates. These results highlight that the use of biocompatible organic chelates positively alters plant physio-biochemical traits contributing to higher Pb-sequestration in sunflower plant parts.

Effect of Irrigation Water Salinity, Sowing Techniques and Mulching on Physio-Biochemical Traits of Fodder Sorghum

Effect of Irrigation Water Salinity, Sowing Techniques and Mulching on Physio-Biochemical Traits of Fodder Sorghum

Effect of Temperature stress on Physio-Biochemical traits of Wheat

Wheat (Triticum aestivum L.) is one of the most crucial staple crops globally, providing essential calories and nutrients to a large portion of the world’s population. However, the production and quality of wheat are increasingly threatened by climate change, particularly by rising temperatures during the grain-filling period, commonly referred to as “terminal heat.” Terminal heat stress, occurring in the final stages of wheat development, can significantly impair grain yield and quality, posing a major challenge to global food security.The present research was conducted at the Student’s Instructional Farm (SIF) of Acharya N.D. University of Agriculture and Technology, Kumarganj, Ayodhya. Laboratory analysis was performed in the Crop Physiology Laboratory. The climate of Ayodhya falls under a semi-arid zone, with a mean annual rainfall of 1100 mm, about 80% of which occurs during the monsoon season (November to April), with occasional showers in winter.Three wheat varieties—PBW-343, HD-2967, and Kundan—were selected for the study, with three replications. PBW-343 and HD-2967 are high-yielding varieties commonly used in the North Eastern Plain Zone (NEPZ) for timely sown conditions, but they are susceptible to temperature stress. Kundan, a popular late-sown variety, is known for its tolerance to high-temperature stress. The treatments were based on three sowing dates: D1 (30th November), D2 (15th December), and D3 (30th December).

Zinc and nitrogen mediate the regulation of growth, leading to the upregulation of antioxidant aptitude, physio-biochemical traits, and yield in wheat plants

An ample amount of water and soil nutrients is required for economic wheat production to meet the current food demands. Nitrogen (N) and zinc (Zn) fertigation in soils can produce a substantial wheat yield for a rapidly increasing population and bring a limelight to researchers. The present study was designed to ascertain N and Zn’s synergistic role in wheat growth, yield, and physio-biochemical traits. A pot experiment was laid out under a complete randomized design with four N levels (N1-0, N2-60, N3- 120, and N4-180 kg ha−1), Zn (T1-0, T2-5, T3-10, and T4-15 kg ha−1) with four replications. After the emergence of the plants, N and Zn fertigation was applied in the soil. The growth traits were considerably increased by combined applications as compared to the sole applications of the N and Zn. The photosynthetic pigments were found maximum due to combined applications of N and Zn, which were positively associated with biomass, growth, yield, and wheat grain quality. The combined application also substantially enhances the antioxidant enzyme activities to scavenge the ROS as H2O2 and reduce lipid peroxidation to protect the permeability of the biologic membranes. The combined higher applications of N and Zn were more responsive to ionic balance in a shoot by maintaining the Na+ for osmotic adjustments, accumulating more Ca2+ for cellular signaling; but, combined applications resulted in K+ reduction. Our present results suggest that appropriate sole or combined applications of N and Zn improve wheat's growth, yield, and antioxidant mechanisms. Previous studies lack sufficient information on N and Zn combined fertigation. We intend to investigate both the sole and combined roles of N and Zn to exploit their potential synergistic effects on wheat.

Unlocking genetic insights: Evaluating wheat RILs for physiobiochemical traits under terminal heat stress conditions

BackgroundThe increasing impacts of heat stress on wheat production due to climate change has entailed the development of heat-resilient crop varieties. To address this, two hundred recombinant inbred lines (RILs) derived from a cross between WH711/WH1021 were evaluated in a randomized block design (RBD) with two replications at CCSHAU, Hisar, during 2018-19 under heat stress and non-stress conditions. Heat stress was induced by altering the date of sowing so that the grain filling stage coincide with heat stress.ResultsHeat stress adversely affects RILs performance, as illustrated by alterations in phenotypic traits. Highest coefficients of variations were recorded for TAA, CTD 1, WUE, CTD 2, Cc and A under non-stress and heat stress conditions whereas gs, WUEi and GY under non-stress and SPAD 1, SPAD 2, GY and NDVI 2 under heat-stress conditions recorded moderate estimates of coefficient of variations. CTD 2, TAA, E, WUE and A displayed a significant occurrence of both high heritability and substantial genetic advance under non-stress. Similarly, CTD 2, NDVI 2, A, WUEi, SPAD 2, gs, E, Ci, MDA and WUE exhibited high heritability with high genetic advance under heat-stress conditions.ConclusionsComplementary and duplicate types of interactions with number of controlling genes were observed for different parameters depending on the traits and environments. RILs 41, 42, 59, 74, 75, 180 and 194 were categorized as heat tolerant RILs. Selection preferably for NDVI 1, RWC, TAA, A, E and WUEi to accumulate heat tolerance favorable alleles in the selected RILs is suggested for development of heat resilient genotypes for sustainable crop improvement. The results showed that traits such as such as NDVI, RWC, TAA, A, E, and WUEi, can be effective for developing heat-resilient wheat genotypes and ensuring sustainable crop improvement.

Genetic Variability and Multivariate Analysis of Physio-Biochemical Traits in Coloured Grape Genotypes

Twenty-two coloured grape accessions were screened for various physio-biochemical traits. The genetic variability and multivariate analysis were carried out for growth and biochemical parameters of grape accessions grown under Pune conditions. High genotypic and phenotypic coefficient of variations were observed in average bunch weight, berry weight, proline and total tannin. All the biochemical parameters showed very high estimated heritability for broad sense (h2b.s.). Higher genetic gain was recorded for average bunch weight, total tannin, proline and protein while low genetic gain was observed in berry diameter and seeds/berry. The multivariate analysis revealed that biochemical parameters could be potentially used for discrimination of grape genotype. The heat map cluster analysis showed higher biochemical parameters in Kali Sahebi, Kishmish Maldovski, Red Ficosa, H-516 and Omania Black. Among the accessions studied, Kali Sahebi performed better concerning growth attributes like average bunch weight, berry diameter, berry and seed weight, days to harvest and biochemical constituents like total phenol, anthocyanin and colour intensity. A very close association was observed between phenotypic and genotypic variation, particularly for biochemical parameters. Therefore, very high heritability and genetic advance were recorded in these parameters. It can be concluded that the parameters governing the very close association between phenotypic and genotypic variation can contribute to the varietal selection and trait-specific varietal development programmes.

Redox priming could be an appropriate technique to minimize drought-induced adversities in quinoa.

The exogenous use of the redox compound (H2O2) plays a significant role in abiotic stress tolerance. The present study investigated various H2O2 application methods (seed priming, foliar spray, and surface irrigation) with varying concentration levels (0 mM, 5 mM, 10 mM, 15 mM, 40 mM, 80 mM, and 160 mM) to evaluate the efficiency of supplying exogenous H2O2 to quinoa under water-deficit conditions. Drought stress reduced quinoa growth and yield by perturbing morphological traits, leading to the overproduction of reactive oxygen species and increased electrolyte leakage. Although all studied modes of H2O2 application improved quinoa performance, surface irrigation was found to be sensitive, causing oxidative damage in the present study. Seed priming showed a prominent increase in plant height due to profound emergence indexes compared to other modes under drought conditions. Strikingly, seed priming followed by foliar spray improved drought tolerance in quinoa and showed higher grain yield compared to surface irrigations. This increase in the yield performance of quinoa was attributed to improvements in total chlorophyll (37%), leaf relative water content (RWC; 20%), superoxide dismutase (SOD; 35%), peroxidase (97%), polyphenol oxidase (60%), and phenylalanine ammonia-lyase (58%) activities, and the accumulation of glycine betaine (96%), total soluble protein (TSP; 17%), proline contents (35%), and the highest reduction in leaf malondialdehyde contents (MDA; 36%) under drought stress. PCA analysis indicated that physio-biochemical traits (proline, SOD, TSP, total chlorophyll, MSI, and RWC) were strongly positively correlated with grain yield, and their contribution was much higher in redox priming than other application methods. In conclusion, exogenous H2O2 application, preferably redox priming, could be chosen to decrease drought-induced performance and yield losses in quinoa.

Impact of ascorbic acid-rich phyto-extracts on growth, yield and physio-biochemistry of okra [Abelmoschus esculentus (L.) Moench.] subjected to drought stress

To overcome the negative effects of drought stress by exogenous application of natural [lemon juice (LJ) and orange juice (OJ)] and synthetic ascorbic acid (AsA) as sources of potential antioxidants was assessed in okra (A. esculentus) under field conditions. The experiment comprised of two cultivars (Sabz Pari and Bhindi Sanwali) those were subjected to different irrigation regimes including control (12 irrigations) as well as water deficit regimes such as D1 (eight irrigations) and D2 (six irrigations). The plants were sprayed with synthetic AsA (150 mg L-1), 50 % LJ and 50 % OJ to both control and water stressed plants after 30 days of seed germination. Foliar applied AsA obtained from varied sources significantly increased the plant biomass, yield characteristics and leaf photosynthetic pigments, total soluble proteins, proline, AsA, total amino acids, total phenolics, total soluble sugars, and activities of POD, CAT and SOD enzymes in okra plants under varying irrigations. Although, in contrast different AsA sources minimized the accumulation of H2O2 and MDA contents in okra plants. In conclusions, LJ and OJ (both 50 %) proved to be more effective than the synthetic AsA (150 mg L-1) in enhancing yield production, plant biomass and different physio-biochemical traits of okra plants subjected to varying water stress levels. Thus, a better organic source of AsA both LJ and OJ may be suggested to overcome the negative influence of water deficiency in plants.

Effects of microbial biofertilizer on growth, physio-biochemical traits, fruit yield, and water productivity of okra under drought stress

Drought poses a major challenge to global agricultural crop production, as it is considered one of the most severe abiotic stresses. Understanding the effects of exogenous microbial biofertilizer on okra [Abelmoschus esculentus (L.) Moench] under drought stress can lead to new strategies for coping with drought conditions. The objective of the study was to examine the effects of microbial biofertilizer on growth, physio-biochemical traits, fruit yield, and water productivity of okra under drought stress. A factorial pot experiment, consisting of eight biofertilizer treatments applied as a soil drench (autoclaved inoculum [control], and inoculation with arbuscular mycorrhizal fungi [AMF], phosphate-solubilizing fungi [PSF], plant growth-promoting rhizobacteria [PGPR], AMF + PSF, AMF + PGPR, PGPR + PSF, and AMF + PSF + PGPR) and three soil moisture regimes (50%, 75%, and 100% field capacity [FC]), was carried out. The results indicated that decreasing soil moisture level severely affected growth and fruit yield of okra. The application of microbial biofertilizer effectively enhanced growth, fruit yield, and physio-biochemical traits of okra under different soil moisture levels. Among the biofertilizer treatments considered, the combined application of AMF and PGPR showed remarkable efficacy in enhancing growth and productivity of okra plants under both 75% and 100% FC conditions. This co-inoculation significantly boosted shoot dry matter by 51%, root dry matter by 73%, fruit yield by 113%, and irrigation water productivity by 122% compared with plants grown without inoculation, irrespective of soil moisture levels. Furthermore, the plants treated with AMF and PGPR exhibited a noteworthy decrease in free proline accumulation by 31% compared with their non-inoculated counterparts. The results suggest that application of both AMF and PGPR biofertilizer to soil is an effective strategy to alleviate moderate drought stress. It is recommended to apply AMF and PGPR in combination to improve okra yield and water productivity under drought stress.

Physio-biochemical responses of rice to smart nitrogen fertilizer nano-hydrogel under drought stress and flooding alternation: An eco-friendly innovative approach to enhance the grain quality

Fertilizer nanohydrogels have been newly introduced to agriculture. Herein, we investigated the effects of smart nitrogen (N) fertilizer nano-hydrogel on rice's physio-biochemical traits and seed quality under different drought stress and flooding conditions. Therefore, a pot experiment was conducted with no N fertilizer delivery 0 g N/kg (NND), with conventional N fertilizer delivery 0.175 g N/kg (CND), and smart N fertilizer delivery 0.175 g N/kg (SND) using three irrigation options: flooding/moderate dryness (FMD, 90% saturation, 30 mm submerged), flooding/sharp dryness (FSD, 70% saturation, 30 mm submerged), and flooding/extreme dryness (FED, 50% saturation, 30 mm submerged). Results showed that rice root's active acquisition area and enzymatic activity were larger in FMD × SND than in FED × NND, as the former combination increased NH4+, NO3−, and TN contents in the soil while the latter reduced their availability. The optimal N nutrition improved the plant cell ultrastructure, and the catalase activity was higher in the FMD × SND (42.0 µg/min) compared to the FED × NND (25.2 µg/min), leading to the alleviation of oxidative cell injury, as verified by lesser radical oxidative sequences and lipid peroxidation. The leaf soluble protein (8.8 mg/g) and sugar (36.9 mg/g) were greater in FMD × SND than in FED × NND (5.3 mg/g), (10.4 mg/g), and (8.3 µg/g h), respectively. Moreover, α-amylase and glutamine synthetase activities were larger in the FMD × SND than in FED × NND. Meanwhile, the FMD × SND resulted in the highest milled rice (87.3%), amylose (17.6%), protein (12.4%), and fatty acids (11.1%). However, the combination of FED × NND led to the lowest milled rice (70.0%), amylose (5.6%), protein (5.3%), and fatty acids (6.6%). This study suggests that the FMD × SND can guarantee physio-biochemical improvements in rice, thereby increasing grain quality.

Elevation-driven modifications in tissue architecture and physiobiochemical traits of Panicum antidotale Retz. in the Pothohar Plateau, Pakistan

Mountainous ecosystems are experiencing rapid shifts in distribution patterns and community structure due to the impacts of climate change and vegetation dynamics. This study offers a comprehensive insight into the adaptive mechanisms of Panicum antidotale to varying elevations within the Pothohar Plateau, shedding light on its survival strategies in climate-affected regions. The findings reveal that populations at lower elevations exhibit maximum height, leaf area, shoot length, and fresh weight, whereas those at higher elevations display significant decrease in these parameters. Moreover, a significant decline in species density, frequency and cover is evident at high elevation. High-elevation populations demonstrate decreased photosynthetic traits, including chlorophyll, carotenoid, and Na and Ca content. Furthermore, there are notable variations in the antioxidant enzyme activity across elevation gradients. Populations at lower elevations exhibit higher activities of superoxide dismutase and peroxidase. Across the gradient, there were significant reductions in root and stem radius, sclerenchyma thickness, bulliform cell size, metaxylem vessels, and phloem area. Populations at moderate elevations displayed thicker lamina and epidermis, larger cortical cells, wider xylem vessels, and increased phloem area; however, these traits declined at higher elevations. High-elevation populations showed thicker lamina and epidermis, extensive sclerenchyma, greater cortical cell area, and higher stomatal density. Photosynthetic traits and enzymatic activity were major contributors at moderate elevations while stem and leaf traits were significantly changed at high elevations. Overall, this research contributes significantly to the broader knowledge of plant adaptation in mountainous ecosystems and stresses the importance of considering elevation as a critical factor in ecological studies.

Mitigating Salinity Stress in Barley (Hordeum vulgare L.) through Biochar and NPK Fertilizers: Impacts on Physio-Biochemical Behavior and Grain Yield

Increased soil salinity significantly inhibits crop production around the world. Over the last decade, biochar has been used in agriculture to improve plant productivity, soil quality, and as an alternative to plant amendment. This study was aimed to study the effect of biochar, NPK, and their combination on the growth, physio-biochemical traits, mineral contents, and grain yield of barley (Hordeum vulgare L.). Thus, a pot factorial experiment based on a completely randomized design with three replications was performed. Experimental treatments included four levels of biochar (0, 2, 5 and 10% of total pot mass), four different NaCl levels (0, 75, 125, and 200 mmol L−1), and with or without NPK fertilizer. The results showed that a negative effect on gas exchange parameters, photosynthetic pigments, SPAD value, minerals contents, and grain yield of barley under salinity treatments. In addition, our funding showed the negative effect on biochemical traits such as proline, soluble sugars, individual sugar, and phenolic compounds. The use of biochar, combined with NPK fertilizers, considerably increases these parameters and especially improves barley grains yield under severe salinity conditions (200 mM) with a dose of 2% and 5% (394.1 and 280.61 g m−2, respectively) of total pot mass. It is concluded that biochar amendment could be a promising practice to enhance barley growth under severe saline irrigation and NPK fertilization regimes.

Predicting and optimizing reactive oxygen species metabolism in Punica granatum L. through machine learning: role of exogenous GABA on antioxidant enzyme activity under drought and salinity stress

BackgroundDrought and salinity stress have been proposed as the main environmental factors threatening food security, as they adversely affect crops' agricultural productivity. As a potential solution, the application of plant growth regulators to enhance drought and salinity tolerance has gained considerable attention. γ-aminobutyric acid (GABA) is a four-carbon non-protein amino acid that accumulates in plants as a response to stressful conditions. This study focused on a comparative assessment of several machine learning (ML) regression models, including radial basis function, generalized regression neural network (GRNN), random forest (RF), and support vector regression (SVR) to develop predictive models for assessing the effect of different concentrations of GABA (0, 10, 20, and 40 mM) on various physio-biochemical traits during periods of drought, salinity, and combined stress conditions. The physio-biochemical traits included antioxidant enzyme activities (superoxide dismutase, SOD; peroxidase, POD; catalase, CAT; and ascorbate peroxidase, APX), protein content, malondialdehyde (MDA) levels, and hydrogen peroxide (H2O2) levels. The non‑dominated sorting genetic algorithm‑II (NSGA‑II) was employed for optimizing the superior prediction model.ResultsThe GRNN model outperformed the other ML algorithms and was therefore selected for optimization by NSGA-II. The GRNN-NSGA-II model revealed that treatment with GABA at concentrations of 20.90 mM and 20.54 mM, under combined drought and salinity stress conditions at 20.86 and 20.72 days post-treatment, respectively, could result in the maximum values for protein content (by 0.80 and 0.69), APX activity (by 50.63 and 51.51), SOD activity (by 0.54 and 0.53), POD activity (by 1.53 and 1.72), CAT activity (by 4.42 and 5.66), as well as lower MDA levels (by 0.12 and 0.15) and H2O2 levels (by 0.44 and 0.55), respectively, in the ‘Atabaki’ and ‘Rabab’ cultivars.ConclusionsThis study demonstrates that the GRNN-NSGA-II model, as an advanced ML algorithm with a strong predictive ability for outcomes in combined stressful environmental conditions, provides valuable insights into the significant factors influencing such multifactorial processes.

Colonization of Satureja hortensis L. (Summer savory) with Trichoderma harzianum alleviates salinity stress via improving physio-biochemical traits and biosynthesis of secondary metabolites

Salinity as the main abiotic stress, influences plant growth, yield, and productivity, especially in arid and semi-arid areas of the world. Researchers are increasingly turning to biological strategies to improve crop tolerance to high salinity levels, such as using endophytic fungus. This study aimed to evaluate the morphological, physiological, and biochemical responses of Summer savory (Satureja hortensis L., Lamiaceae) plants to inoculation with Trichoderma harzianum (an endophytic fungus) under various levels of salinity (0, 50, 100, and 150 mM NaCl) in a pot-based experiment. The results showed that Summer savory inoculated with T. harzianum exhibited improved growth compared with salt-stressed and non-stressed plants (up to 93.47%). Furthermore, inoculation with T. harzianum caused a restoration in the content of chlorophyll a, b, and carotenoids up to 1.47-fold. Both non-enzymatic and enzymatic antioxidants were enhanced in the presence of salt stress, and T. harzianum further increased their level, approximately up to 66.67%. Salinity caused an obvious increase in hydrogen peroxide, malondialdehyde, and electrolyte leakage (up to 3.04-fold), but their content began to decline when the plants were inoculated with T. harzianum (with reduction factor up to 41.43%). Additionally, T. harzianum-inoculated plants maintained higher levels of proline, total soluble sugar content (up to 1.46-fold), and volatile organic compounds compared with control and salt-stressed plants. Overall, the findings of this study suggest that inoculation with T. harzianum can enhance the salt tolerance of S. hortensis by improving growth via modulation of biochemical, physiological, and secondary metabolites biosynthesis. Therefore, T. harzianum may be a promising biological strategy for improving crop productivity under saline conditions.

Gut microbiota and metabolite insights into anti-obesity effect of carboxymethyl pachymaran in high-fat diet mice

Poria cocos, a well-known traditional Chinese edible-medicinal mushroom, has shown many beneficial health effects. In this study, we investigated the anti-obesity and hyperlipidemia effect of carboxymethyl pachymaran (CMP) in high-fat diet mice, with a focus on gut microbiota and their metabolites. Results demonstrated that CMP notably decreased fat weights and serum lipid, improved glucose tolerance, and reduced lipid droplet level in hepatic tissue. CMP markedly reduced the ratio between Firmicutes and Bacteroidetes, decreased the abundances of Dubosiella newyorkensis and Faecealibaculum rodentium, but increased the abundances of Akkermansia muciniphila and Bifidobacterium pseudolongum. In addition, CMP significantly promoted the production of butyric acid (BA) while decreased the levels of hepatic bile acids. Association analysis showed that physio-biochemical traits were negatively associated with A. muciniphila and BA, whereas showed a positive correlation to F. rodentium and differential bile acids. Together, CMP can alleviate obesity and related complications by modulating gut microbiota and metabolites.

Responses of submerged macrophytes to different particle size microplastics and tetracycline co-pollutants at the community and population level

Microplastics (MPs) and antibiotics are ubiquitous in aquatic ecosystems, and their accumulation and combined effects are considered emerging threats that may affect biodiversity and ecosystem function. The particle size of microplastics plays an important role in their combined effects with antibiotics. Submerged macrophytes are crucial in maintaining the health and stability of freshwater ecosystems. However, little is known about the combined effects of different particle size of MPs and antibiotics on freshwater plants, particularly their effects on submerged macrophyte communities. Thus, there is an urgent need to study their effects on the macrophyte communities to provide essential information for freshwater ecosystem management. In the present study, a mesocosm experiment was conducted to explore the effects of three particle sizes (5 µm, 50 µm, and 500 µm) of polystyrene-microplastics (PSMPs) (75 mg/L), tetracycline (TC) (50 mg/L), and their co-pollutants on interactions between Hydrilla verticillata and Elodea nuttallii. Our results showed that the effects of MPs are size-dependent on macrophytes at the community level rather than at the population level, and that small and medium sized MPs can promote the growth of the two test macrophytes at the community level. In addition, macrophytes at the community level have a stronger resistance to pollutant stress than those at the population level. Combined exposure to MPs and TC co-pollutants induces species-specific responses and antagonistic toxic effects on the physio-biochemical traits of submerged macrophytes. Our study provides evidence that MPs and co-pollutants not only affect the morphology and physiology at the population level but also the interactions between macrophytes. Thus, there are promising indications on the potential consequences of MPs and co-pollutants on macrophyte community structure, which suggests that future studies should focus on the effects of microplastics and their co-pollutants on aquatic macrophytes at the community level rather than only at the population level. This will improve our understanding of the profound effects of co-pollutants in aquatic environments on the structure and behavior of aquatic communities and ecosystems.

Plant growth-promoting rhizobacteria and biochar as bioeffectors and bioalleviators of drought stress in faba bean (Vicia faba L.).

Plants are subjected to a variety of abiotic stressors, including drought stress, that are fatal to their growth and ability to produce under natural conditions. Therefore, the present study was intended to investigate the drought tolerance potential of faba bean (Vicia faba L.) plants under the co-application of biochar and rhizobacteria, Cellulomonas pakistanensis (National Culture Collection of Pakistan (NCCP)11) and Sphingobacterium pakistanensis (NCCP246). The experiment was initiated by sowing the inoculated seeds with the aforementioned rhizobacterial strains in earthen pots filled with 3kg of sand-mixed soil and 5% biochar. The morphology of biochar was observed with highly porous nature, along with the detection of various essential elements. The biochemical and physiological data showed that phenolic compounds and osmolytes were adversely affected by the induction of drought stress. However, the application of biochar and rhizobacteria boosted the level of flavonoids on average by 52.03%, total phenols by 50.67%, soluble sugar by 82.85%, proline by 76.81%, glycine betaine by 107.25%, and total protein contents by 89.18% in all co-treatments of biochar and rhizobacteria. In addition, stress indicator compounds, including malondialdehyde (MDA) contents and H2O2, were remarkably alleviated by 54.21% and 47.03%, respectively. Similarly, the amplitude of antioxidant enzymes including catalase, peroxidase, superoxide dismutase, ascorbate peroxidase, and guaiacol peroxidase was also enhanced by 63.80%, 80.95%, 37.87%, and 58.20%, respectively, in all co-treatments of rhizobacteria and biochar. Conclusively, biochar and rhizobacteria have a magnificent role in enhancing the drought tolerance potential of crop plants by boosting the physio-biochemical traits and enhancing the level of antioxidant enzymes.

Seed priming with melatonin induces rhizogenesis and modulates physio-biochemical traits in high-yielding rice (Oryza sativa L.) genotypes

Melatonin has been recognized as a regulator of plant growth and development. The present study aims to optimize the appropriate dosage of melatonin through seed priming, taking into account a range of physio-biochemical traits. Two distinct rice genotypes with divergent yield potentials, namely N-22 (drought-tolerant) and IR-64 (drought-susceptible) were subjected to priming with varying concentrations of melatonin (0, 60, 90, 120, 150, and 180 ppm). Our study revealed that, at the 120-ppm melatonin concentration, IR-64 demonstrated superior germination rates, root biomass, shoot biomass, chlorophyll content, and total antioxidant activity as compared to N-22 genotype. Conversely, N-22 displayed enhanced shoot length and root length in contrast to IR-64, at the 120-ppm dosage. Additionally, the melatonin concentration of 120 ppm exhibited profound efficacy in reshaping root-shoot architecture (RSA) in both genotypes. Consequently, the optimal melatonin dosage for effective seed priming was established as 120 ppm. Further, priming the seeds of IR-64 and N-22 with melatonin (120 ppm) holds the potential to enhance rice yield through the augmentation of overall growth and development. These findings enrich our comprehension of melatonin's influence on rice seed priming and pave the path for future explorations into the fundamental mechanisms governing these effects.