The electron trapping in AlGaN/GaN high-electron mobility transistors (HEMTs) with iron (Fe)-doped buffer is investigated through Drain Current Transient (DCT) measurements and TCAD physics-based 2D device simulations. The DCT characterization reveals two prominent deep-level electron traps E1 (∼0.5 eV) and E2 (∼0.6 eV) in the AlGaN/GaN HEMT. The measured DCT spectrum is analyzed at different trap-filling pulse durations (10 µs–100 ms) to obtain the information of trapping kinetics. As the first step in the simulation, the TCAD physical model parameters are calibrated by matching the simulated DC characteristics with the experimental data. It is shown that the TCAD model incorporating the acceptor-type trap at EC – 0.5 eV in the GaN buffer quantitatively reproduces the measured DCT spectra over the temperature range of 25–100 °C. To explore the buffer trapping effects, the simulated DCT is inspected by varying the activation energy, capture cross section, and concentration of the buffer trap.