Physicochemical Stability Research Articles

Green synthesized chitosan-coated iron oxide nanocomposite using Cissus quadrangularis plant extract for antibacterial, antioxidant and anticancer applications

Metal oxide nanoparticles (NPs) have demonstrated their potential to eliminate a wide range of drug-resistant bacterial pathogens and cancer cells in recent years. Iron oxide (Fe3O4) NPs have received significant attention due to their wide range of uses in the field of biomedicine. In specific, biopolymer-encapsulated Fe3O4 NPs were applied in cancer therapy owing to their biocompatibility and biodegradability. The current investigation describes a bio-inspired approach to synthesize chitosan-based Fe3O4 nanocomposite (NC) employing Cissus quadrangularis (CQ) plant extract in a sustainable manner. The physicochemical, morphological, compositional, surface texture and thermal stability properties of the biopolymer-coated nanocomposites were characterised using FT-IR, UV–vis, XRD, XPS, HR-SEM, TEM, TGA, BET and TGA analyses. The antibacterial efficacy was examined in strains of Escherichia coli and Staphylococcus aureus, which verified that the growth of bacterial pathogens was inhibited by a substantial increase in the concentration of composites. The DPPH assay results were utilized to assess the free radical scavenging activity. The in vitro anticancer activity was found to be significant against the human osteosarcoma (MG-63) cell line, with an IC50 value of 54 ± 0.50 μg/mL. The enhanced biocompatibility of NCs was confirmed by cytotoxic testing on human RBCs and normal L929 fibroblast cells.

Long-term stability of esketamine in polypropylene syringes at 5 ± 3°C

ObjectiveEsketamine (Vesierra) is a molecule, used alone or in combination, to induce and maintain general anaesthesia and to relieve pain in emergency medicine. The aim of this study is to...

The Condition of Contemporary Murals in Sun-Exposed Urban Environments: A Model Study Based on Spray-Painted Mock-Ups and Simulated Light Ageing

The present work investigates the physicochemical stability of spray paints when irradiated with artificial solar light (at spectral range 300–800 nm). This research highlights the importance of understanding the materials used in street art and public murals, recognising them as a significant component of contemporary cultural heritage. By examining the stability and degradation of spray paints toward solar light exposure, the study aims to contribute to the preservation of contemporary murals, which reflect current social and cultural narratives. A physicochemical approach was employed for the study of spray paints’ physical and thermal properties, as well as the effect of specific photochemical ageing reactions/processes. The photochemical ageing results were compared with reference (unaged) samples. Specifically, a multi-technique approach was applied using stereo microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle measurement, colorimetry, glossimetry, differential scanning calorimetry (DSC), UV-Vis spectroscopy, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and pyrolysis-GC/MS (Py-GC/MS). The photodegradation of the spray paints occurred from the first 144 h of solar light irradiation, resulting in changes in morphology, colour, gloss, roughness, and wettability. Regarding photochemical stability, ageing seems to affect the binders more than the synthetic organic pigments and the inorganic fillers. In particular, acrylic binders showed small chemical changes, whereas the alkyd, nitrocellulose, and styrene binders underwent severe chemical modification. The results suggest that simulated daylight irradiation prompts the migration of additives toward the surface of the spray paint films. In addition, the results of the analyses on the white spray paints in comparison with the coloured paints (from the same manufacturer) showed that there seems to be an active distinct photoageing mechanism involving titanium dioxide, but the whole issue needs further investigation.

Electricity Harvesting from Water Evaporation on Hierarchical Pore Gradient Silica Aerogel-Based Generators.

In this study, the electric energy harvesting capability of the hierarchical pore gradient silica aerogel (HPSA) is demonstrated due to its unique porous structure and inherent hydroxyl groups on the surface. Taking advantage of the positively charged surface of unwashed HPSA credited by the preparation strategy, poly(4-styrene sulfonic acid) (PSS) can be spontaneously adsorbed onto unwashed HPSA and shows gradient distribution due to the pore-gradient structure of HPSA. By virtue of the gradient distribution and the stronger ionization of PSS, PSS-modified HPSA (PSS-HPSA) shows enhanced electricity generation performance from natural water evaporation with an average output voltage of 0.77 V on an individual device. The water evaporation-induced electricity property of PSS-HPSA can be maintained in the presence of a low concentration of salt. The desirable salt resistance capability benefits from the unique 3D hierarchical porous structure of HPSA which ensures rapid water replenishment so as to effectively avoid the salt accumulation. The HPSA-based devices with the advantages of unique porous structure, easy functionalization, good physicochemical stability, good salt resistance capability, and eco-friendliness show great potential as water evaporation-induced electricity generators.

The synergistic effect of Cd-doped and S-vacancies in CdxZn1-xIn2S4 2D nanosheets for high-performance triethylamine sensing

Ternary metal sulfides with suitable band gaps, high physicochemical stability, and unique two-dimensional (2D) nanostructures are expected to be the next-generation high-performance gas sensors following the MOS type. Doping engineering is utilized as an effective strategy to improve the semiconductor surface activity and enhance its gas-sensitive properties. In this paper, the energy band structure and surface chemical oxygen of ZnIn2S4 (ZIS) materials was tuned by selectively introducing substitutional Cd to replace the Zn sites in ZIS crystals. Meanwhile, the introduction of Cd-ions brings more abundant S vacancy defects, enhances the acid-base interactions at the interface, and pushes the extent of surface redox reactions. In addition, by combining the strong adsorption of ZIS to triethylamine, the CdxZn1-xIn2S4 nanosheets achieved highly improved sensing properties, including better response (63.38–100 ppm), enhanced selectivity (STEA/sother = 12.9), and accelerated response/recovery (4 s/32 s). The results confirm the feasibility of developing low-cost, high-performance 2D metal sulfide gas sensing materials through rational structural design and optimization.

Monolithic conjugated microporous polymers with antibacterial activity for air filtration

The air pollution caused by particulate matters (PMs) poses a severe threat to the human health. In particular, the fine particles with diameter smaller than 2.5 μm (PM2.5) can be carriers of various bacteria and viruses, accelerating spread of epidemic. In this work, we synthesized two robust and flexible conjugated microporous polymer (CMP) aerogels for PMs removal, via one-step coupling polymerization of arylethynylenes and aryl halides with different molecular configuration. The resulting CMP aerogels comprise of hollow nanotubes with 3D porous network and high surface areas up to 660 m2/g. The efficiency of CMP aerogels for PM2.5 and PM10 was 99.5 ± 0.3 % and 99.8 ± 0.1 % with a long-term durability. In combination with their physicochemical stability and inherent surface hydrophobicity, a high efficiency for PM2.5 filtration more than 99.6 ± 0.1 % also can be obtained at a high humidity at room temperature both for the two CMP aerogels. In addition, benefiting from the rich alkynyl groups in skeletons, post-modification of the resulting CMP aerogels with thiol-yne chemistry followed by silver ion uptake gives rise to antibacterial materials with outstanding activity for gram-positive strains far superior to other porous organic polymer materials. This work provides a new avenue to fabricate bifunctional CMPs derived porous aerogels for air filtration with high performance and antibacterial activity, and is expected to expand the application of CMPs in environmental purification.

Co-encapsulation of vitamin E and quercetin by soybean lipophilic proteins based on pH-shifting and ultrasonication: Focus on interaction mechanisms, structural and physicochemical properties

This study explored the mechanism of interaction of pH-shifting combined ultrasonication and its effect on soybean lipophilic proteins (SLP) and the potential of modified SLP as the carrier for vitamin E (VE) and quercetin (QU). The spectroscopy results revealed that both VE and QU changed the SLP conformation and exposed hydrophobic groups. The loading rates of VE and QU by SLP with alkaline pH-shifting combined with ultrasonication (300 w,20 min) were 86.91% and 75.99%, respectively. According to the antioxidant analysis, with an increase in the ultrasonication power, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity of the samples increased, where the DPPH and ABTS radical scavenging capacity of sample SQV-6 were 70.90% and 63.43%, respectively. The physicochemical properties, microstructure, and stability of the SLP–VE–QU complex improved significantly. Overall, the present findings broadened the application of simple structural carriers for co-encapsulating functional factors.

Physicochemical Stability of Doravirine (Pifeltro®): Characterization of Main Degradation Products and Assessment of Stability of Tablets Repackaged in Single-Dose Unit Containers.

Doravarine (DOR) is an antiviral drug with a marketed authorization for the management of occupational blood and body fluid exposure. The currently existing packaging, consisting of multiple unit bottles comprising 30 tablets, is not fully appropriate for daily nominative dispensing at the hospital. This study aims at assessing the impact of the change in packaging on the key attributes of the drug: assay, impurity profile, and dissolution. As the first step, which is not fully depicted in the literature, the main potential impurities that could appear during storage (i.e., degradation products (DPs) of DOR) were characterized using a forced degradation protocol followed by an LC-MS/MS analysis. These results paved the way for in silico toxicological assessment and targeted degradation product profiling. Based on this study, the assessment of the implication of repackaging on the formation of DOR's degradation products should be a primary focus.

Characterization of pectin-zein nanoparticles encapsulating tanshinone: Antioxidant activity, controlled release properties, physicochemical stability to environmental stresses

Tanshinone compounds, natural antioxidants found in the roots of Salvia subg Perovskia plants, offer various health benefits and can serve as natural food additives, replacing synthetic antioxidants. In this study, the nanoparticles were created using the antisolvent method, which were then evaluated for their antioxidant and antibacterial properties, as well as their ability to release tanshinone and withstand environmental stress. The results of the study demonstrated a significant improvement in the antioxidant capabilities of tanshinone with the nanoparticle coating. The T/Z/P NPs exhibited enhanced tanshinone release under simulated gastrointestinal conditions compared to T/Z nanoparticles. These nanoparticles displayed remarkable stability against fluctuations in environmental pH and thermal conditions. The study also revealed that the critical flocculation concentration of the system was 0.5 M of salt. Furthermore, the T/Z/P NPs showed good stability during storage at 4°C for 30 days, making them an excellent candidate for use in various food products.

Preparation of novel highly stable fluorinated microporous polyketone networks through direct C–H arylation polycondensation for efficient natural gas separation

Two novel hyper-cross-linked fluorinated microporous polyketone networks (FMPN-1 and FMPN-2) with permanent porosity and narrow pore size distribution were synthesized via a facile “one-step” polycondensation between the trifluoroacetophenone-based monomers and 1,3,5-triphenylbenzene. The result polymers were investigated by FTIR, solid state NMR, EDX, XRD, TGA, SEM, and TEM et al. The geometry configurations difference of building blocks resulted in the polyketone networks exhibiting the BET special surface areas from 622 to 706 m2 g−1 and total volume from 0.58 to 0.66 cm3 g−1. What's more, ideal adsorbed solution theory (IAST) analysis showed that the two polymers possessed good C3H8/CH4, C2H6/CH4 selectivities at 298K/1 bar. Together with the excellent thermal and physicochemical stabilities, the microporous fluorinated polyketone networks obtained in this work showed promising applications in adsorption/separation for C1–C3 hydrocarbons.

Amorphous High‐Entropy Alloy Interphase for Stable Lithium Metal Batteries

AbstractThe unstable anode/electrolyte interphase induces severe lithium dendrite growth hindering the practical application of lithium metal batteries. The lithium alloy interphase presents a promising strategy for regulating Li+ plating/stripping behavior. However, binary or ternary alloys are insufficient to address various challenges in lithium metal batteries and the high temperature required for alloy preparation hampers their direct applications on lithium metal surfaces. In this study, a high‐entropy alloy (HEA) interphase is developed on lithium metal surfaces via room‐temperature magnetron sputtering, showcasing multifunctional advantages in regulating Li+ plating/stripping behavior. The cocktail effect of the (HEA) facilitated the formation of a homogeneous amorphous interphase with abundant lithiophilic sites and magnetic properties, promoting uniform Li+ nucleation and deposition. Furthermore, the high mechanical strength and corrosion resistance of HEA provided physicochemical stability for the lithium anode interphase, consistently suppressing dendrite growth. Consequently, lithium metal anodes with HEA interphases exhibited robust cycling performance lasting over 4000 h at 2 mA cm−2. The LFP full battery demonstrated high‐capacity retention of 90% with an average Coulombic efficiency of 99.7%. Thus, the HEA interphases on lithium metal surfaces offer controllable regulation of Li+ deposition behavior through high‐entropy manipulation, opening novel strategies for stable lithium metal batteries.

An electrospun iron oxychloride/polyacrylonitrile nanofibrous membrane with superhydrophilic and excellent regeneration properties: Achieving superior oil-in-water emulsion separation

Superhydrophilic polymer membranes are widely used in oil-water separation due to their excellent wettability, high efficiency, low cost, and good biocompatibility. However, challenges such as fouling adhesion and inadequate stability persist during the treatment of oily wastewater. Herein, a novel iron oxychloride/polyacrylonitrile (FeOCl/PAN) nanofibrous membrane with superhydrophilicity and remarkable regenerability was successfully fabricated through simple electrospinning technique, which maintained good physicochemical stability under various harsh conditions with the uniform distribution of FeOCl on PAN fibers. The surface morphology and chemical compositions of the optimized FeOCl/PAN membrane were studied by several characterization techniques. The introduced FeOCl nanocrystals through electrospinning endowed the membrane with a rougher surface structure, significantly improving the hydrophilicity of the FeOCl/PAN nanofibrous membrane. In the cross-flow isooctane-in-water emulsion separation experiment, the initial water flux recovery rate (FRR) of the membrane increased from 92 % for the pure PAN membrane to around 99 %. Meanwhile, the optimized FeOCl/PAN nanofibrous membrane achieved an emulsion (isooctane) flux of approximately 632.9 L m−2 h−1, with an oil droplet removal rate surpassing 99.4 %. Furthermore, by cleaning the membrane with H2O2 (60 mM, pH = 3), the FeOCl/PAN membrane exhibited an exceptionally high water flux recovery rate (∼100 %) after high-concentration paraffin-in-water emulsion (4000 mg L−1) separation. In addition, the FeOCl/PAN membrane also demonstrated excellent antifouling performance in bovine serum albumin (BSA) pollution experiment. In summary, this work might provide a unique strategy for preparing advanced functional membranes with superior efficiency and durability for emulsified oily wastewater treatment.

Pickering emulsions of zein nanoparticles co-stabilized by Tween 20: An effective strategy to stabilize citral in low pH environment

The demand for surfactant-free emulsions is growing significantly in food industry. Herein, we report the fabrication of zein nanoparticles, modified their surface hydrophobicity with Tween 20, and used them to stabilize various formulations of Pickering emulsions designated as ZT0 %, ZT5 %, ZT10 %, ZT15 %, ZT20 %, and ZT25 %, for preventing the acid-catalysed degradation of citral at 25 °C. Our results suggest that, as the concentration of Tween 20 increases, the droplet size of the emulsions first increases and then decreases, whereas the mechanical strength continuously increases. Citral encapsulated in ZT25 % shows maximum physicochemical stabilization as determined by visual, olfactory and HPLC analysis, and when released in saline shows maximum antioxidant activity due to the favorable charge characteristics on Pickering emulsifiers, and the existence of hydrophobic ⇋ electrostatic interaction. As compared to prior literature, our work represents a significant advancement in fabrication of zein nanoparticles (ZNPs), their stabilization using Tween 20 and the formation of appropriate Pickering emulsions stabilized by Tween 20 modified ZNPs appropriate for modifying the interfacial characteristics of the emulsion for enhanced physicochemical stability, excellent aroma profile, controlled release rate, and improved antioxidant activity of encapsulated citral under harsh acidic conditions. Moreover, the novel Chemical Kinetic Method has been applied to analyze and interpret the citral stability as a function of interfacial parameters. The study, therefore presents a novel particle-surfactant complex interface, having great potential for the encapsulation, protection, and release of citral from the acidic food/pharmaceutical formulations.

Aesthetic synthesis and comparative study of graphitic carbon nitride for energy storage applications

ABSTRACT Graphitic carbon nitride (gCN) has emerged as a suitable 2D material for various applications, such as photocatalysis, energy storage, and conversion. Due to their abundance of active sites, their unique layered structure, metal-free characteristics, high physicochemical stability, generous in nature, being environmentally friendly and having unique electrical characteristics. In this study, we use a one-step pyrolysis procedure to address these limitations and propose robust, low-cost, and scalable ways to synthesise materials. The synthesised gCN material graphitic phase is confirmed by X-ray diffraction analysis, the layered structure of the sp2 hybridised C-N bonding features is shown by FT-IR analysis. FESEM analyses provide insights into the morphology of gCN. Elemental identification and oxidation states were investigated using X-ray photoelectron spectroscopy (XPS). The electrochemical properties of gCN are performed using a two-electrode system with PVA/KOH electrolyte. The gCN nanostructure shows a specific capacitance of 183.20 F/g at a current density (CD) of 2 A/g, an energy density (ED) of 30.44 Wh/kg, and a power density (PD) of 2739.6 W/kg. Furthermore, the gCN nanostructure shows an excellent cycle life with 87.11% capacitance retention and 98.13% coulombic efficiency. These investigations clarify the electrochemical properties of gCN as an electrode material for supercapacitors.

LOW-TEMPERATURE CALORIMETRIC CHARACTERIZATION OF DEEP EUTECTIC SOLVENTS WITH ADDED WATER

Deep eutectic solvents (DES) are combinations of hydrogen bonds donors and acceptors, characterized by significantly lower freezing temperatures than their components. NADES (with natural, biologically compatible components) are becoming popular for a wide applications variety, for their wide solvent capacities, non-toxicity, biodegradability, and inexpensive components. Applications include new means for bioactive compounds extraction and enzymatic reactions, polymer solubilizing and a variety of physicochemical processes, including those taking place at low temperature. Four DES (β-alanine and DL-malic or citric acid (plus water) and choline chloride and ethylene glycol or glycerol) were investigated by differential scanning calorimetry (DSC) at low temperature. Pure DES behave completely reversibly, proof of physicochemical stability. Alanine-DES present glass transitions between -40 and -80°C. Meanwhile, choline-DES show no thermal events between 0° and -80°C. Increasing water produce glass transitions in all DES at decreasing temperatures, and devitrifications and freezing events. Choline-DES show no effects up to 20% water. However, alanine-DES show strong water effects, possibly phase separation into different water populations. Calcium alginate-sucrose beads were used as structural model for diffusion systems. Beads were incubated in DES (20 min/20 hours) and their low-temperature properties characterized. Short incubation periods were enough to radically alter their thermal behavior.

Bacterial cellulose nanocrystals or nanofibrils as Pickering stabilizers in low-oil emulsions: A comparative study

This investigation assessed the potential of bacterial cellulose (BC) in two distinct forms, nanocrystals (BC-NC) and oxidized nanofibrils (BC-NF), as stabilizers for low oil-in-water emulsions (1 % v/v). The research explored the impact of ionic strength and BC concentration on the physico-chemical characteristics, stability, and rheological properties of those emulsions. Nanofibrils had diameters ranging from 25 to 146 nm and lengths in the micrometer range, while nanocrystals varied in length from 133 to 870 nm and in diameter from 20 to 60 nm. Both BC-NF and BC-NC exhibited high zeta potential values (>−45 mV) and contact angles of 30-31°, indicating stability. Both nanocelluloses were effectively used as stabilizers in Pickering emulsions, namely in low-oil systems, producing small emulsion droplets with sizes between 1.42 and 4.13 μm. Further results revealed that ionic strength influenced emulsion stability, with both BC-NF and BC-NC preferentially located on the surfaces of emulsion droplets in the presence of salt, as demonstrated by microscopy images. The presence of BC at the interface contributed to creating a more robust barrier against coalescence and Ostwald ripening, influencing droplet size and rheological properties. Higher BC concentrations (1 %) increased emulsion stability in the absence of salt, while at lower BC concentrations (0.5 %), salt concentration was determinant for the long-term stability of the emulsions. These findings provide valuable insights into the production of Pickering emulsions using nanocelluloses, highlighting the advantages of bio-based nanomaterials for applications in the food industry.

Phycocyanin Encapsulation in Carboxymethyl Chitosan and Whey Protein Isolate as a Strategy to Enhance Physicochemical Stability as a Food Coloring: A Review

Color additives, also known as food colorings, are substances added to food and beverages to enhance their visual appeal and consumer acceptance. The color of a food product serves as an indicator of attributes like flavor, safety, and nutritional value. Unlike other natural colors, blue-colored fruits and vegetables are rare, making blue associated with synthetic food coloring. Phycocyanin (PC), a natural blue pigment found in Spirulina, has garnered attention for its various biological properties, including antioxidant and anti-inflammatory activities. However, its industrial application limited by susceptibility to factors like high temperatures and pH variations during processing. Encapsulation, a technique involving the trapping of bioactive ingredients in carriers, can address these issues. In this study, chitosan derivatives, Carboxymethyl Chitosan (CMC)-Whey Protein Isolate (WPI) used as a coating for complexes in the encapsulation of phycocyanin. Encapsulation employed to improve pigment stability, and the study investigates the impact of ratios, pH, and CaCl2 concentration addition as a crosslinking agent. The selection of the appropriate CMC-WPI ratio is crucial for achieving good physicochemical properties, high encapsulation efficiency, and survivability against gastrointestinal and storage conditions. The encapsulated phycocyanin demonstrates stability across various pH values, with the concentration of CaCl2 influencing particle diameter and shape.

Production and Quality Assessment of Plant-based Yoghurt from Coconut Milk Fortified with Date Syrup

Aim: To evaluate the physicochemical, microbial, sensory attributes and storage stability of coconut yoghurt fortified with date syrup. Methodology: Yoghurt was prepared from coconut milk and date syrup. Five samples were formulated as follows: T0 (100% cow milk), T1 (100% coconut milk), T2 (85% coconut milk, 15% date syrup), T3 (80% coconut milk, 20% date syrup), T4 (75% coconut milk, 25% date syrup) and T5 (70% coconut milk, 30% date syrup) The yoghurt samples were subjected to physicochemical and microbial analysis using standard methods. The sensory attributes assessed were appearance, aroma, taste, consistency and overall acceptability. The pH, titratable acidity and viscosity were evaluated during 4 weeks of storage at refrigerated condition (4- 6o c). Results: From the result, pH, titratable acidity, viscosity, and total solid ranged from 4.50 - 4.41%, 0.82 – 0.94%, 12.0 -72.40 cP and 11.64 – 18.81% respectively. These parameters increased significantly (p<0.05) with increase in the proportion of date syrup. The result showed a significant increase in ash, protein, carbohydrate, and fiber with a corresponding decrease in fat and moisture content as the proportion of date syrup increased. The total bacterial count ranged from 6.95 - 8.20cfu/ml, with Yeast, molds and coliforms undetected. The produced yoghurt samples all have quality attributes of animal milk yoghurt and better storage stability. Conclusion: Therefore, production of commercial yoghurt using plant-based milk (coconut milk) blended with nutritiously rich sweeteners like date fruit is encouraged.

A First Insight into the Developability of an Immunoglobulin G3: A Combined Computational and Experimental Approach.

Immunoglobulin G 3 (IgG3) monoclonal antibodies (mAbs) are high-value scaffolds for developing novel therapies. Despite their wide-ranging therapeutic potential, IgG3 physicochemical properties and developability characteristics remain largely under-characterized. Protein-protein interactions elevate solution viscosity in high-concentration formulations, impacting physicochemical stability, manufacturability, and the injectability of mAbs. Therefore, in this manuscript, the key molecular descriptors and biophysical properties of a model anti-IL-8 IgG1 and its IgG3 ortholog are characterized. A computational and experimental framework was applied to measure molecular descriptors impacting their downstream developability. Findings from this approach underpin a detailed understanding of the molecular characteristics of IgG3 mAbs as potential therapeutic entities. This work is the first report examining the manufacturability of IgG3 for high-concentration mAb formulations. While poorer conformational and colloidal stability and elevated solution viscosity were observed for IgG3, future efforts controlling surface potential through sequence-engineering of solvent-accessible patches can be used to improve biophysical parameters that dictate mAb developability.

Assessment of Antimicrobial and Cytotoxic Activities of Liposomes Loaded with Curcumin and Lippia origanoides Essential Oil.

(1) Introduction: Curcumin and Lippia origanoides essential oils have a broad spectrum of biological activities; however, their physicochemical instability, low solubility, and high volatility limit their therapeutic use. Encapsulation in liposomes has been reported as a feasible approach to increase the physicochemical stability of active substances, protect them from interactions with the environment, modulate their release, reduce their volatility, improve their bioactivity, and reduce their toxicity. To date, there are no reports on the co-encapsulation of curcumin and Lippia origanoides essential oils in liposomes. Therefore, the objective of this work is to prepare and physiochemical characterize liposomes loaded with the mixture of these compounds and to evaluate different in vitro biological activities. (2) Methods: Liposomes were produced using the thin-layer method and physiochemical characteristics were calculated. The antimicrobial and cytotoxic activities of both encapsulated and non-encapsulated compounds were evaluated. (3) Results: Empty and loaded nanometric-sized liposomes were obtained that are monodisperse and have a negative zeta potential. They inhibited the growth of Staphylococcus aureus and did not exhibit cytotoxic activity against mammalian cells. (4) Conclusions: Encapsulation in liposomes was demonstrated to be a promising strategy for natural compounds possessing antimicrobial activity.