Soil Physicochemical Properties Research Articles

Seasonal Effects of Wildfires on the Physical and Chemical Properties of Soil in Andean Grassland Ecosystems in Cusco, Peru: Pending Challenges

Soils are a valuable renewable resource on human timescales, and they interact with distinctive grassland ecosystems characterized by unique biodiversity and essential provision of ecosystem services, such as water supply and carbon sequestration. However, knowledge of the effects of wildfires on soil properties and nutrient availability in the Andes remains limited. Andean grasslands are currently one of the ecosystems of the Peruvian Andes most affected by wildfires. Our objective is to analyze the effect of fire activity on the physicochemical properties of soil and analyze its social context in Cusco, in the southern Andes of Peru. Soil samples were collected during five periods, spanning both the dry and rainy seasons, to characterize changes in soil properties and monitor vegetation recovery post-fire in two local communities dedicated to livestock activities. The vegetation restored after the wildfire was measured by the “step transect” method. Post-fire changes in soil properties indicate slight increases in pH, electrical conductivity, organic matter, nitrogen, phosphorus, and potassium during the onset of the rainy season; thereafter, a gradual reduction in these values was observed. This reduction can be attributed to leaching associated with the seasonal rainfall and runoff regime. Our findings indicate that one-year post-fire, the biomass in burned areas is reduced to 30–46% of the biomass in unburned areas. A complete regeneration is likely to occur in up to 4 years; this assertion is supported by the perceptions of the affected population, as expressed in interviews conducted in the two farming communities. These results are significant for decision-makers formulation of policies and regulations regarding grasslands and their seasonal restoration.

Earthworms alleviate microplastics stress on soil microbial and protist communities

Microplastic (MP) pollution can exert significant pressure on soil ecosystems, however, the interactive effects of MPs on soil bacterial, fungal and protist communities remains poorly understood. Soil macrofauna, such as earthworms, can be directly affected by MPs, potentially leading to a range of feedbacks on the soil microbial community. To address this, we conducted a microcosm experiment to examine the effects of conventional (i.e., polyethylene, polystyrene) and biodegradable MPs (i.e. PBAT, polylactic acid) on the structure of the soil bacterial, fungal, and protist communities in the presence or absence of earthworms. We found that MP contamination negatively affected the diversity and composition of soil microbial and protist communities, with smaller-sized conventional MPs having the most pronounced effects. For example, compared with the unamended control, small-sized polyethylene MPs both significantly reduced the Shannon diversity of soil bacteria, fungi, and protist by 4.3 %, 37.0 %, and 9.1 %, respectively. Biodegradable MPs increased negative correlations among bacteria, fungi, and protists. However, earthworms mitigated these effects, enhancing the diversity and altering the composition of these communities. They also increased the niche width and stability of the soil microbial food web network. Our study indicated that earthworms help attenuate the response of soil microorganisms to MPs stress by influencing the diversity and composition of soil microorganisms and soil physicochemical properties and underscores the importance of considering macrofauna in MPs research.

Mediterranean beech forests: Thinning and ground-based skidding are found to alter microarthropod biodiversity with no effect on litter decomposition rate

Despite the high ecological value of Mediterranean beech forests, very little is known about the implications of forest operations on soil microarthropod biodiversity and litter decomposition rate. There is also no information concerning the amount of time needed for disturbed forest soil to recover and return to the pre-harvesting conditions. Silvicultural treatments are scheduled about every ten to fifteen years, without taking into account the amount of time necessary for recovery. The purpose of this study was to determine this information. The study started by selecting three study sites located along the Italian Apennine, each including a chronosequence of three forest parcels: one harvested in 2021, one harvested in 2012, and a control parcel which had not been harvested within the last forty years. In the harvested parcels we investigated skid trails which are classified as disturbed, and soil not affected by the passage of a machine which is classified as undisturbed. Thus in each study area there were five experimental treatments including the control area. The soil physico-chemical properties were assessed for each treatment. These included bulk density, penetration resistance, shear resistance, organic matter content, and soil microarthropod biodiversity which was assessed by the QBS-ar index (Soil Biological Quality based on microarthropods, a qualitative index measuring the quality of soil according to the biodiversity of the microarthropod community). We further established a litter decomposition experiment, using teabags as reference material, to check the differences among treatments in litter decomposition rates. We used linear mixed-effects models to investigate the effects of the experimental treatment on the physico-chemical and biological features, and relationships among QBS-ar index and soil physico-chemical features. We analysed the effects of the experimental treatment on the litter decomposition rate using generalised linear-mixed effects models. A significant effect caused by machine passage was found in the recently harvested parcels on soil penetration resistance (∼+70 % comparing to the control), shear resistance (∼+35 %), and QBS-ar index (-25 %). Effects of machine passage on soil bulk density and organic matter content were not significant. All the investigated variables in the skid trails returned to values similar to those recorded in the control after ten years. We did not find effects of any soil physico-chemical features on QBS-ar index. Finally, no effect of the experimental treatment was found on the litter decomposition rate, suggesting that a stronger magnitude of soil disturbance is needed to affect this complex biogeochemical process. Although the initial impact was highly significant, it was found that a 10-year period is sufficient for recovering the investigated soil features in the upper soil layer. This was observed in Mediterranean beech forests when forest operations were done with low-weight machines and a limited number of machine passes on the skid trails. Thus, it can be concluded that planning another operation 10–15 years after the previous thinning, as is currently done, remains in line with the time needed for the forest soil to recover.

Soil Physicochemical Properties under Selected Avocado Cultivars in Ethiopian Smallholder Agroforestry

This study investigated the impact of three avocado cultivars on selected soil physicochemical properties in Central Ethiopia, to enhance the knowledge on the influence of avocado cultivars on soil physicochemical properties, and assist smallholders in cultivar selection in agroforestry. Trees planted in farmers´ fields 8 years earlier were revisited. Soil samples were collected from 0–20 cm and 20–40 cm depth at three radial distances from trees (1 m and 2 m from tree trunk, and at 5 m from the canopy edge as a control). Soil texture, bulk density, moisture content, pH, electrical conductivity, soil organic carbon, total nitrogen and available phosphorus were determined. Soil moisture content and electrical conductivity were overall higher and bulk density was lower (p < 0.05) under the canopies of the avocado trees than in the control and soil organic carbon, total nitrogen and available phosphorus tended to increase in spite of nutrient inputs to the control whilst the trees were unfertilized. Differences between the studied cultivars were small, but Hass tended to have the largest impact on soil nutrient levels, whilst Ettinger and Nabal tended to have a somewhat larger effect on the soil organic carbon concentration. Integrating these avocado cultivars on farms can improve soil fertility in the study area. However, for optimal agricultural soil health and sustainable avocado production, cultivar and site-specific soil management practices must be applied.

Biomass Ash as a Substitute for Lime and Its Impact on Grassland Soil, Forage, and Soil Microbiota

In this eight-year grassland field trial, we compared the fertilization effects of biomass ashes (BMAs) and carbonated lime (CaCO3) in combined application with cattle slurry (CS). Our study focused on plant coverage, forage yield, and quality, as well as soil physicochemical and microbiological properties. The fertilization strategies included CS mixed with BMA or CaCO3 applied three times a year and a separate annual application of ash or CaCO3, independent of CS. Samplings were performed in 2010, 2014, and 2018. Despite an absence of observable effects on soil, microbial properties, and forage quality, CS application, with or without BMA/CaCO3, resulted in higher forage yields compared to the unfertilized control and plots receiving only ash or CaCO3. Forage properties remained consistent across treatments. However, the combined application of CS with both ash and CaCO3 led to a reduction in volatile organic compounds, total carbon, total nitrogen, nitrate, and electrical conductivity in the soil from 2010 to 2018. Additionally, the relative abundance of specific microbial families (Nitrosomonadaceae, Acidothermaceae, Bacillaceae, and Peptostreptococcaceae) varied based on whether soils received a single amendment or a combination thereof. Our findings suggest that BMA is a valuable substitute for traditional liming agents, regardless of the application mode.

Kiwifruit-Agaricus blazei intercropping effectively improved yield productivity, nutrient uptake, and rhizospheric bacterial community

Intercropping systems have garnered attention as a sustainable agricultural approach for efficient land use, increased ecological diversity in farmland, and enhanced crop yields. This study examined the effect of intercropping on the kiwifruit rhizosphere to gain a deeper understanding of the relationships between cover plants and kiwifruit in this sustainable agricultural system. Soil physicochemical properties and bacterial communities were analyzed using the Kiwifruit-Agaricus blazei intercropping System. Moreover, a combined analysis of 16S rRNA gene sequencing and metabolomic sequencing was used to identify differential microbes and metabolites in the rhizosphere. Intercropping led to an increase in soil physicochemical and enzyme activity, as well as re-shaping the bacterial community and increasing microbial diversity. Proteobacteria, Bacteroidota, Myxococcota, and Patescibacteria were the most abundant and diverse phyla in the intercropping system. Expression analysis further revealed that the bacterial genera BIrii41, Acidibacter, and Altererythrobacter were significantly upregulated in the intercropping system. Moreover, 358 differential metabolites (DMs) were identified between the monocropping and intercropping cultivation patterns, with fatty acyls, carboxylic acids and derivatives, and organooxygen compounds being significantly upregulated in the intercropping system. The KEGG metabolic pathways further revealed considerable enrichment of DMs in ABC transporters, histidine metabolism, and pyrimidine metabolism. This study identified a significant correlation between 95 bacterial genera and 79 soil metabolites, and an interactive network was constructed to explore the relationships between these differential microbes and metabolites in the rhizosphere. This study demonstrated that Kiwifruit-Agaricus blazei intercropping can be an effective, labor-saving, economic, and sustainable practice for reshaping bacterial communities and promoting the accumulation and metabolism of beneficial microorganisms in the rhizosphere.

Effect of Different Levels of NPK, Biochar and Azotobacter on Physico-chemical Properties of Soil on Cowpea (Vigna unguiculata)

In light of this, the following goals are present in the experiment "Effect of Different Levels of NPK and Biochar, Azotobacter on Physico-chemical Properties of Soil and Yield Attributes of Cowpea to calculate the impact of various NPK, Biochar, and Azotobacter dosages on the physical-chemical characteristics of soil. An excavated soil sample from the experimental site revealed that the land topography ranged from nearly level to sloped by 1% to 6%, with soil area falling into the Inceptisol order. The soil texture was sandy loam, with sand percentages of 62.65%, silt percentages of 21.09, and clay percentages of 16.26. The pH of soil was 6.89, and its electrical conductivity (EC) was non-saline (0.42 ds m-1). Organic carbon content was low to medium, available nitrogen was low to medium (280.78 kg ha-1), available phosphorus was 17.34 kg ha-1, and available potassium was 168.16 kg ha-1. Two factors with three levels of @NPK 0, 50, and 100% ha-1, three levels of @Biochar 0, 50, and 100% ha-1, and a randomized block design were used in the statistical analysis. During field testing, nine different treatments were used; the best outcomes were significant. The results indicate that the physical and chemical parameters of the soil, including the cumulative mean values for bulk density (1.39 and 1.41 mg m-3), particle density (2.46 and 2.47 mg m-3), and soil pH (6.89 and 6.91), attained their maximum in T1 (Absolute control) at depths of 0-15 cm and 15-30 cm, respectively. Additionally, the percentage pore space was found to be 48.22% and 47.39%, and the water holding capacity was found to be 44.14% and 45.34%), electrical conductivity (EC) was measured at 0.49 ds m-1 and 0.52 ds m-1, the percentage of organic carbon was determined to be 0.40%), and the available nitrogen was found to be (299.78 kg ha-1 and 295.76 kg ha-1, available phosphorus was (23.78 kg ha-1 and 21.98 kg ha-1), and the available potassium was (179.25 kg ha-1 and 176.56 kg ha-1).

Phytoremediation Characterization of Heavy Metals by Some Native Plants at Anthropogenic Polluted Sites in Jeddah, Saudi Arabia

Many anthropogenic activities have lately resulted in soil adulteration by heavy metals (HMs). The assessment of native plant species that grow in the polluted environments is of great importance for using these plants in phytoremediation techniques. This study was conducted in three industrial regions in Jeddah city, Wadi Marik, Bahra, and Khumrah, to assess the HM contamination level in them. This study also evaluated the phytoremediation ability of nine plant species collected from the studied regions. Soil physicochemical properties of the studied sites were investigated. Nine HMs, aluminum (Al), nickel (Ni), zinc (Zn), cobalt (Co), iron (Fe), lead (Pb), manganese (Mn), chromium (Cr), and barium (Ba), have been evaluated in the collected soil, plant shoots, and root samples. Total thiol concentration in the plant shoots and roots was determined. The phytoremediation indexes, such as bioaccumulation factor (BCF) and translocation factor (TF), were estimated. The results show that the soil of all the explored sites was sandy and slightly alkaline. It was found that Ni, Pb, and Cr were above the international permissible limit in all soil samples. The Wadi Marik region recorded the highest HM concentration compared to the other sites. In the Bahra region, Fe, Zn, Co, and Mn in all collected soil samples were below internationally permissible levels. In Khumrah, the highest concentration of Zn was found in the soil sample collected around F. indica plants, while Fe, Co, and Mn in all collected soil samples were below the international permissible limit. Depending on the BCF calculations, most of the investigated species showed phytostabilization ability for most of the studied HMs. Of them, E. indica, T. nubica, and P. divisum recorded the highest BCF values that ranged from 16.1 to 3.4. The BCF values of the studied HMs reduced in the order of Cr &gt; Zn &gt; Mn &gt; Co &gt; Ba &gt; Fe &gt; Al &gt; Pb. Phytoextration of Co and Cr could be achieved by P. oleracea and F. indica, which showed TF values that reached 6.7 and 6.1, respectively. These plants showed high potential for phytoremediation and can be suggested as protective belts close to the contaminated regions of Jeddah.

Effect of sugarcane bagasse biochar on soybean germination and initial growth

Biochar is known as an excellent soil conditioner and a powerful carbon sequestration tool. However, to expand its use, it is necessary to understand potential adverse effects. To better address this issue, biochar was prepared from sugarcane bagasse. The effect of biochar on the germination of soybean seeds was evaluated, and a greenhouse experiment was conducted to assess the response of soybean seedlings to biochar application at different rates (CK, 0% w/w; BC1, 1% w/w; BC3, 3% w/w; and BC5, 5% w/w). Thirty days after sowing, growth parameters, soil physicochemical attributes, and microbiota were evaluated. The biochar exhibited a low O/C ratio (0.18) and an irregular porous surface, which promoted improvements in the soil's physical, chemical, and biological attributes. It also demonstrated no phytotoxic effects in germination tests for application rates up to 50 t/ha. The results of the soybean cultivation showed that the BC3 and BC5 treatments significantly increased the fresh mass of the aerial part by 64% and 86%, respectively, compared to CK. These treatments also significantly increased the fresh mass of the root system by 23% and 423%, respectively. These results highlight the potential of biochar in promoting early soybean development.

Using the pedogenic oxide ratio in distinguishing volcanic soil weathering intensity in subtropical region

Pedogenic oxide ratio (POR) helps identify and understand pedogenic processes. However, POR is seldom used to study and distinguish the weathering intensity of volcanic soils that are moderately weathered in the subtropical region. The research was conducted by collecting thirty-three subtropical volcanic soils from the northeastern Tatung Volcanic Group (TVG) region in Yangmingshan National Park, near Taipei, located in northern Taiwan. These volcanic soils could be grouped into two groups with different weathering intensities based on three weathering indices, pedogenic Fe-oxides ratios (PORs) - Feo/Fed, ((Fed-Feo)/Fet), and total reserve in bases (TRB): the highly weathered (HW) group and the lower weathered (LW) group. The two PORs show significant differences between the two groups, but not for TRB, which indicates the PORs are a good indicator for providing quantitative measurements of weathering intensity. Except for the soil bulk density (BD) meeting andic soil properties, the soils of the HW group have a lower content of Al plus 1/2 Fe content (by ammonium oxalate) (Alo + 1/2Feo) and phosphorus (P) retention, and soils of the LW group only have a lower P retention. Within those soil physicochemical properties and selective dissolution analysis results and index values, Alo + 1/2Feo, pyrophosphate extractable C (Cp), P retention, and dithionite-citrate-bicarbonate (DCB) extractable aluminum (Ald) are four critical factors for the discrimination of the soils. The soils in the study area still have relict volcanic soil properties after moderately weathered. Our research results indicate that the PO and POR are good indicators of moderately weathered volcanic soils in the subtropical region, allowing for a more precise assessment of the soil weathering process, in addition to quantitative measurements of weathering intensity.

Dissolve organic matter of mature chicken compost contributes to the protection of microorganisms from the stress of heavy metals

Dissolved organic matter (DOM), as the most active component of manure and compost, can influence heavy metal forms and microbial community structure by changing the physicochemical properties of soils. This study employed DOM extracted from fresh chicken manure (FDOM) and mature compost (MDOM) and evaluated their impacts on the physicochemical properties, heavy metal forms, and microbial communities of chernozem and dark brown soil through UV-Vis, EEM-PARAFAC, and high-throughput sequencing. The results showed that all DOM treatments decreased soil pH except for the MDOM at 150 mg C/kg applied to chernozem (CLM). MDOM treatments were more favorable in increasing soil available phosphorus content and the degree of humification of soil DOM, and FDOM treatments were more favorable in increasing soil dissolved organic carbon content and decreasing the degree of humification of soil DOM. Except for CLM treatment, the other DOM treatments decreased the content of stable state Pb, increased the risk of Pb migration and biotoxicity, and FDOM treatments were more pronounced. In addition, DOM treatments decreased the Shannon diversity of two soils and the Chao1 index in chernozem. However, DOM at 300 mg C/kg could increase the abundance of soil eutrophic and heavy metals tolerant dominant phyla (Proteobacteria and Bacteroidota) as well as dominant genera (Sphingomonas, Lysobacter, and Arthrobacter). This study provides a comprehensive understanding of the risk of manure and compost application in heavy metals-polluted soils and its interaction with microorganisms.

Effects of Land Use Change on Soil Aggregate Stability and Erodibility in the Karst Region of Southwest China

Differences in land use type and chronological age affect soil properties and plant community characteristics, which may influence soil structural stability and erodibility. However, knowledge on the effects of soil physicochemical properties on soil aggregate stability and erodibility at different land use years is limited. This study selected five land use types: corn field (Year 38th-y), corn intercropped with cabbage field (Year 38th-y + b), fruit and meridian forest (Year 6th-jgl), naturally restored vegetation (Year 6th-zr), and artificial forest (Year 7th-rgl) in the karst landscape of the Chishui River Basin in Yunnan Province. We aimed to identify the influencing factors of soil stability and erodibility under different land use time series. The results indicated that the mean weight diameter (MWD), the geometric mean diameter (GMD), and soil structural stability index (SSI values) were highest in Y6th-zr and lowest in Y7th-rgl. Conversely, the erodibility K value was lowest in Y6th-zr, suggesting that the soil structure in Y6th-zr exhibited greater stability, whereas soil stability in Y7th-rgl was lower. Redundancy and throughput analyses revealed that organic carbon and water-stable aggregates &gt; 2.0 mm content had higher vector values. Soil bulk density, total nitrogen, organic carbon, and soil texture content were the main factors contributing to soil stability variation (0.338–0.646). Additionally, total nitrogen, organic carbon, total phosphorus, and soil texture content drove the variation in K values (0.15–1.311). Natural vegetation restoration measures can enhance soil structure to a certain extent. These findings highlight changes in soil aggregate stability and erodibility over different land use durations. The research results have important theoretical and practical significance for understanding the differences in soil erosion and soil restoration under different land use patterns in the karst landscapes of southwest China.

Study on Soil Fertility Characteristics of Walnut Orchards with Different Parent Materials and Soil Types in Gyaca County, Tibet

Walnut trees on the Tibetan Plateau have sustainable and nutritious characteristics. They grow naturally without the use of any chemical fertilizers and pesticides. Therefore, the soil fertility of walnut orchards is a key factor influencing walnut yield and quality. This experiment measured and analyzed the basic soil nutrient content, microbial biomass carbon (MBC), nitrogen (MBN), phosphorus (MBP), and leaf nutrient indicators in five types of representative walnut orchard soil parent materials in Gyaca County to clarify the fertility characteristics of different soil types and their correlations with walnut nutrients, providing a basis for the sustainable development of the plateau walnut industry. The results showed that there were significant differences in soil organic matter (SOM), total nitrogen (TN), available nutrient contents, and microbial activity among soil types with different parent materials. The comprehensive representation of available nutrient levels in the soils is as follows: gray-cinnamon soil and calcareous alluvial soil &gt; mountain meadow soil &gt; plateau prairie soil and brush prairie soil. The average MBC, MBN, and MBP contents in the five soils were 368.8 mg/kg, 28.4 mg/kg, and 23.6 mg/kg, respectively, and the content in the topsoil (0–20 cm) was higher than that of the subsoil (20–40 cm). The total nitrogen (PN), total phosphorus (PP), and total potassium (PK) contents in walnut leaves were found to be closely related to the soil physicochemical properties and microbial activity, among which five indicators, including soil TN, available nitrogen (AN), SOM, MBC, and slowly available potassium (SAK), had the greatest impact on leaf nutrient levels. These findings are valuable for the future management and development of walnut orchards. Therefore, for different soil parent materials and soil types, improving the soil fertility and actively applying nitrogen-rich organic fertilizers should be prioritized to improve the yield and quality of Tibetan Plateau walnuts.

The impact of mineral fertilisers on the physicochemical properties of soil in maize cultivation

The use of mineral fertilisers is a critical factor in modern agriculture, significantly influencing the physicochemical properties of soil, which in turn affects crop yield and quality. Understanding these impacts is essential for sustainable agricultural practices. This study aimed to determine the effects of different types and quantities of mineral fertilisers on the physicochemical properties of soil in the context of maize cultivation. The research involved experimental field trials with varying applications of mineral fertilisers. Soil samples were collected at different stages of maize growth and analysed for parameters such as pH, electrical conductivity, organic matter content, and nutrient availability (nitrogen, phosphorus, potassium). The study employed a range of methods to investigate the effects of mineral fertilisers on soil properties, including soil preparation, application of various types and doses of fertilisers, maize planting, plant growth monitoring, and analysis of soil physicochemical characteristics. The application of mineral fertilisers led to significant changes in soil pH, with some fertilisers causing acidification and others increasing alkalinity. Fertilised plots showed increased electrical conductivity, indicating a rise in soluble salt content. Variations in organic matter content were observed, dependent on the type and dosage of fertilisers used. It was determined that fertilised plots exhibited elevated levels of nitrogen, phosphorus, and potassium, directly correlating with the type and quantity of fertiliser applied. The highest maize yield was achieved with balanced applications of nitrogen-phosphorus-potassium (NPK) fertilisers, underscoring the importance of balanced nutrient management. These findings provide valuable insights for optimising fertiliser use, which may contribute to improved soil health, increased maize yield, and sustainable agricultural practices

Autoclaving is at least as effective as gamma irradiation for biotic clearing and intentional microbial recolonization of soil.

Sterilization is commonly used to remove or reduce the biotic constraints of a soil to allow recolonization by soil-dwelling organisms, with autoclaving and gamma irradiation being the most frequently used approaches. Many studies have characterized sterilization impacts on soil physicochemical properties, with gamma irradiation often described as the preferred approach, despite the lower cost and higher scalability of autoclaving. However, few studies have compared how sterilization techniques impact soil recolonization by microorganisms. Here, we compared how two sterilization approaches (autoclaving; gamma irradiation) and soil washing impacted microbial recolonization of soil from a diverse soil inoculum. Sterilization method had little impact on microbial alpha diversity across recolonized soils. For sterile soil regrowth microcosms, species richness and diversity were significantly reduced by autoclaving relative to gamma irradiation, particularly for fungi. There was no impact of sterilization method on bacterial composition in recolonized soils and minimal impact on fungal composition (P = 0.05). Washing soils had a greater impact on microbial composition than sterilization method, and sterile soil regrowth had negligible impacts on microbial recolonization. These data suggest that sterilization method has no clear impact on microbial recolonization, at least across the soils tested, indicating that soil autoclaving is an appropriate and economical approach for biotically clearing soils.IMPORTANCESterilized soils represent soil-like environments that act as a medium to study microbial colonization dynamics in more "natural" settings relative to artificial culturing environments. Soil sterilization is often carried out by gamma irradiation or autoclaving, which both alter soil properties, but gamma irradiation is thought to be the gentler technique. Gamma irradiation can be cost prohibitive and does not scale well for larger experiments. We sought to examine how soil sterilization technique can impact microbial colonization, and additionally looked at the impact of soil washing which is believed to remove soil toxins that inhibit soil recolonization. We found that both gamma-irradiated and autoclaved soils showed similar colonization patterns when reintroducing microorganisms. Soil washing, relative to sterilization technique, had a greater impact on which microorganisms were able to recolonize the soil. When allowing sterilized soils to regrow (i.e., persisting microorganisms), gamma irradiation performed worse, suggesting that gamma irradiation does not biotically clear soils as well as autoclaving. These data suggest that both sterilization techniques are comparable, and that autoclaving may be more effective at biotically clearing soil.

Microenvironment heterogeneity affected by anthropogenic wildfire-perturbed soil mediates bacterial community in Pinus tabulaeformis forests.

In recent years, the frequency and intensity of anthropogenic wildfires have drastically increased, significantly altering terrestrial ecosystems worldwide. These fires not only devastate vegetative cover but also impact soil environments and microbial communities, affecting ecosystem structure and function. The extent to which fire severity, soil depth, and their interaction influence these effects remains unclear, particularly in Pinus tabulaeformis forests. This study investigated the impact of wildfire intensity and soil stratification on soil physicochemical properties and microbial diversity within P. tabulaeformis forests in North China. Soil samples were collected from different fire severity zones (Control, Light, Moderate, High) and depths (topsoil: 0-10 cm; subsoil: 10-20 cm). Analyses included measurements of soil pH, organic carbon (SOC), total nitrogen (TN), and other nutrients. Microbial diversity was assessed using 16S rRNA gene sequencing. Our findings revealed significant variations in soil pH, SOC, TN, and other nutrients with fire severity and soil depth, profoundly affecting microbial community composition and diversity. Soil pH emerged as a critical determinant, closely linked to microbial α-diversity and community structure. We found that fire severity significantly altered soil pH (p = 0.001), pointing to noteworthy changes in acidity linked to varying severity levels. Topsoil microbial communities primarily differentiated between burned and unburned conditions, whereas subsoil layers showed more pronounced effects of fire severity on microbial structures. Analysis of bacterial phyla across different fire severity levels and soil depths revealed significant shifts in microbial communities. Proteobacteria consistently dominated across all conditions, indicating strong resilience, while Acidobacteriota and Actinobacteriota showed increased abundances in high-severity and light/moderate-severity areas, respectively. Verrucomicrobiota were more prevalent in control samples and decreased significantly in fire-impacted soils. Chloroflexi and Bacteroidota displayed increased abundance in moderate and high-severity areas, respectively. Correlation analyses illustrated significant relationships between soil environmental factors and dominant bacterial phyla. Soil organic carbon (SOC) showed positive correlations with total nitrogen (TN) and alkaline hydrolysable nitrogen (AN). Soil pH exhibited a negative correlation with multiple soil environmental factors. Soil pH and available phosphorus (AP) significantly influenced the abundance of the phylum Myxococcota. Soil water content (WC) significantly affected the abundances of Acidobacteriota and Actinobacteriota. Additionally, ammonium nitrogen (NH4 +-N) and nitrate nitrogen (NO3 --N) jointly and significantly impacted the abundance of the phylum Chloroflexi. This study highlights the significant long-term effects of anthropogenic wildfires on soil microenvironment heterogeneity and bacterial community structure in P. tabulaeformis forests in North China, 6 years post-fire. Our findings demonstrate that fire severity significantly influences soil pH, which in turn affects soil nutrient dynamics and enhances microbial diversity. We observed notable shifts in the abundance of dominant bacterial phyla, emphasizing the critical role of soil pH and nutrient availability in shaping microbial communities. The results underscore the importance of soil stratification, as different soil layers showed varying responses to fire severity, highlighting the need for tailored management strategies. Future research should focus on long-term monitoring to further elucidate the temporal dynamics of soil microbial recovery and nutrient cycling following wildfires. Studies investigating the roles of specific microbial taxa in ecosystem resilience and their functional contributions under varying fire regimes will provide deeper insights. Additionally, exploring soil amendments and management practices aimed at optimizing pH and nutrient availability could enhance post-fire recovery processes, supporting sustainable ecosystem recovery and resilience.

Effect of nitrogen addition on soil net nitrogen mineralization in topsoil and subsoil regulated by soil microbial properties and mineral protection: Evidence from a long-term grassland experiment

Soil net nitrogen mineralization (Nmin), a microbial-mediated conversion of organic to inorganic N, is critical for grassland productivity and biogeochemical cycling. Enhanced atmospheric N deposition has been shown to substantially increase both plant and soil N content, leading to a major change in Nmin. However, the mechanisms underlying microbial properties, particularly microbial functional genes, which drive the response of Nmin to elevated N deposition are still being discussed. Besides, it is still uncertain whether the relative importance of plant carbon (C) input, microbial properties, and mineral protection in regulating Nmin under continuous N addition would vary with the soil depth. Here, based on a 13-year multi-level field N addition experiment conducted in a typical grassland on the Loess Plateau, we elucidated how N-induced changes in plant C input, soil physicochemical properties, mineral properties, soil microbial community, and the soil Nmin rate (Rmin)-related functional genes drove the responses of Rmin to N addition in the topsoil and subsoil. The results showed that Rmin increased significantly in both topsoil and subsoil with increasing rates of N addition. Such a response was mainly dominated by the rate of soil nitrification. Structural equation modeling (SEM) revealed that a combination of microbial properties (functional genes and diversity) and mineral properties regulated the response of Rmin to N addition at both soil depths, thus leading to changes in the soil N availability. More importantly, the regulatory impacts of microbial and mineral properties on Rmin were depth-dependent: the influences of microbial properties weakened with soil depth, whereas the effects of mineral protection enhanced with soil depth. Collectively, these results highlight the need to incorporate the effects of differential microbial and mineral properties on Rmin at different soil depths into the Earth system models to better predict soil N cycling under further scenarios of N deposition.

Assessing Soil and Land Suitability of an Olive–Maize Agroforestry System Using Machine Learning Algorithms

Exponential population increases are threatening food security, particularly in mountainous areas. One potential solution is dual-use intercropped agroforestry systems such as olive (Olea europaea)–maize (Zea mays), which may mitigate risk by providing multiple market sources (oil and grain) for smallholder producers. Several studies have conducted integrated agroforestry land suitability analyses; however, few studies have used machine learning (ML) algorithms to evaluate multiple variables (i.e., soil physicochemical properties and climatic and topographic data) for the selection of suitable rainfed sites in mountainous terrain systems. The goal of this study is therefore to identify suitable land classes for an integrated olive–maize agroforestry system based on the Food and Agriculture Organization (FAO) land suitability assessment framework for 1757 km2 in Khyber Pakhtunkhwa province, Pakistan. Information on soil physical and chemical properties was obtained from 701 soil samples, along with climatic and topographic data. After determination of land suitability classes for an integrated olive–maize-crop agroforestry system, the region was then mapped through ML algorithms using random forest (RF) and support vector machine (SVM), as well as using traditional techniques of weighted overlay (WOL). Land suitability classes predicted by ML techniques varied greatly. For example, the S1 area (highly suitable) classified through RF was 9%↑ than that of SVM, and 8%↓ than that through WOL. The area of S2 (moderately suitable) classified through RF was 18%↑ than that of SWM and was 17%↓ than the area classified through WOL; similarly, the S3 (marginally suitable) class area via RF was 27%↓ than that of SVM, and 45%↓ than the area classified through WOL. Conversely, the area of N2 (permanently not suitable class) classified through RF and SVM was 6%↑ than the area classified through WOL. Model performance was assessed through overall accuracy and Kappa Index and indicated that RF performed better than SVM and WOL. Crop suitability limitations of the study area included high elevation, slope, pH, and large gravel content. Results can be used for sustainable intensification in mountainous rainfed regions by expanding intercrop agroforestry systems in developing nations to close yield gaps.

Morphological and Physico-chemical Properties of Soils of Amtur-3 Micro-Watershed on Schistose Landscape in Bailhongal taluk of Belagavi District, Karnataka, India

Aims: To assess the morphological and physico-chemical properties of two soil series viz., KKT (Kabulayathkatti tanda) and SDH (Singadahalli) of Amtur-3 micro-watershed developed on schistose landscape in northern transition zone of Karnataka. Place and Duration of Study: The study was carried out in the Amtur-3 micro-watershed (4D5B8t04) located in Bailhongal taluk of Belagavi district, Karnataka during 2022-2024. Methodology: Horizon-wise soil samples were collected from the two representative pedons viz., KKT (Kabulayathkatti tanda) and SDH (Singadahalli) soil series, were identified, characterized and classified up to family level according to revisions in Soil Taxonomy using morphological, physical and chemical properties. Results: The soils of these two series were sandy clay loam to clay in texture, neutral in soil reaction and had low salt content. Calcium and magnesium were the dominant cations followed by sodium and potassium. Both the soil series belonged to the order Inceptisol.

Soil physico-chemical properties and yield response of cucumber (Cucumis sativus L.) To 2,4-Epibrasinolide under water stress

Dry season production of cucumber is often limited by water availability. Here, we explored the pleiotropic role of 2,4-epibrassinolide (BR) in ameliorating water stress based on changes in soil physico-chemical properties and fruit yield of cucumber under water stressed-conditions. The effects of four water stress levels-WL0, [No stress/control: 860 mL (100% daily irrigation)], WL1 [640 mL (75% daily irrigation)], WL2 [430 mL (50% daily irrigation)] and WL3 = [210 mL (25% daily irrigation)] in factorial combination with foliarly-applied two BR levels [with (BR1= 150 and 250 mL) and without (BR0= 0 mL)] were studied. The soil hydraulic conductivity (Ks) decreased substantially under the water-stressed condition. Soil pH, total nitrogen, available phosphorus, exchangeable bases, effective cation exchange capacity and base saturation were adversely (p ≤ 0.05) affected by water stress, but the exchangeable acidity (EA) was little changed. Water-stressed soil treated with BR showed a significant reduction in EA, increased Ks and nutrient availability. Although dry matter, biological yield, moisture content of the leaves and fruit yield were severely reduced by water stress, treatment with BR increased these by about 89, 27, 41 and 95%, respectively. Light interception was considerably improved under BR treatment as evidenced from the photosynthetically active radiation which increased by 42%. Over all, the increased fruit yield was a result of reduced soil acidity, increased nutrient availability and better use of light energy. Foliar application with BR could sustain growth and fruit yield of cucumber up to 50% of daily irrigation requirement.