Physicochemical Analysis Research Articles

Characterization of groundwater and cores in the decommissioned acid in-situ leach uranium mining area: Enlightenment for uranium contaminated groundwater remediation

Acid In-situ Leach (AISL) is predominant technique for uranium mining in sandstone-type deposits in China. However, its impact on the biogeochemical conditions of sandstone uranium deposits remains poorly understood. In this study, we performed detailed physicochemical analyses of groundwater, as well as mineralogical, chemical and biogeochemical analyses of cores collected from the decommissioned AISL uranium mining area in the Yili Basin, northwestern China. After AISL uranium mining, U(IV) minerals and pyrite were oxidized and dissolved. The groundwater in the AISL-mined uranium deposit (AISLMUD) became highly acidic and oxidizing after mining ceased. Pyrite, which significantly influences the migration, transformation and immobilization of uranium, was minimal in all cores. Kaolinite, chlorite, quartz, K-feldspar, albite and muscovite exhibited poor adsorption capacity of U(VI) under strong acid conditions. The AISLMUD demonstrated limited capacity to retain U(VI) under highly acid conditions. Furthermore, high-throughput sequencing analysis revealed a substantial reduction in both microbial diversity and abundance in the cores. Microorganisms associated with U(VI) reduction were detected in the cores, with the exception of sulfate reduction bacteria. However, the results of microbial incubation showed that U(VI) reduction-related bacteria could not be in-situ activated by the addition of electron donors under the environmental stresses induced by prolonged acid in-situ leaching. These findings cast doubt on the feasibility of remediating uranium-contaminated groundwater with a pH below 3 through in-situ activation of U(VI) reduction-related bacteria. This research holds significant implications for evaluating the suitability of existing methods and developing new approaches for the restoration of AISLMUD.

Determining the effect of high hydrostatic pressure on the refrigerated stability of avocado puree

The present study aimed to extend refrigerated stability of locally grown avocado fruit by applying High Hydrostatic Pressure (HHP) treatment. HHP was applied at 600 MPa for 3 min to avocado puree and stored at 4 °C for 28 days. Physicochemical (colour CIE L*, a*, b*, total oil amount, chlorophyll, pH, moisture) and microbiological (mesophilic, yeast-mould) analyses were performed at seven-day intervals on both control (untreated) and HHP-treated sample packs. It could be judged that HHP treatment effectively controlled the colour indices, preventing undesired changes during the cold storage period of avocado puree. In conclusion, this study demonstrated that HHP-processed avocado puree exhibited stability for 28 days compared to unprocessed puree at 4 °C. The shelf-life stability of avocado puree under chilled conditions was extended from 7 to 28 days.Graphical

New peripherally-restricted CB1 receptor antagonists, PMG-505–010 and −013 ameliorate obesity-associated NAFLD and fibrosis

The endocannabinoid system plays a crucial role in metabolic regulation, prompting the investigation of cannabinoid type 1 receptor (CB1R) antagonists for obesity and its complications like non-alcoholic fatty liver disease (NAFLD). Concerns over psychiatric side effects led to the development of peripheral CB1R antagonists that circumvent the blood-brain barrier (BBB). In this study, we synthesized PMG-505–010 and PMG-505–013 as peripherally restricted CB1 receptor antagonists by modifying rimonabant to minimize BBB penetration. Physicochemical analysis confirmed their reduced lipophilicity and increased polarity compared to rimonabant, indicating limited brain exposure. Molecular docking studies revealed similar binding modes to rimonabant at CB1R, characterized by robust hydrophobic interactions. Functionally, they acted as CB1R antagonists and inverse agonists, effectively reversing CP55,940-induced cAMP inhibition. In a murine model of obesity-related NAFLD, PMG-505–010 and −013 improved metabolic profiles, including fasting blood glucose levels and dyslipidemia. They also ameliorated hepatic injury, steatosis, and inflammation, evidenced by reduced liver enzymes, lipid peroxidation, hepatic lipid levels, and inflammatory cytokine levels. Notably, these compounds inhibited hepatic fibrosis by reducing extracellular matrix (ECM) deposition and altering fibrosis-related gene and protein expressions. In conclusion, PMG-505–010 and PMG-505–013 hold promise for treating obesity-related liver diseases, including NAFLD and fibrosis, through selective peripheral CB1R targeting, potentially avoiding CNS-related side effects seen with earlier CB1R antagonists.

Synthesis of Pd-decorated ZnO nanocomposites with improved gas-sensitive properties for acetone detection

ZnO/Pd nanocomposites containing 0.5–3.0 mol.% palladium and possessing improved gas-sensitive properties for VOC’s detection were prepared using solvothermal synthesis. The synthesised powders have been characterised using various methods of physicochemical analysis (DSC/TGA, XRD, SET/TEM, XPS). It is shown that Pd@PdO nanoparticles (4–7 nm in diameter) are localised on the surface of ZnO nanoparticles (40–50 nm in size). According to the combined XRD and XPS data, it was found that palladium is in partially oxidised state, and the nanocomposite has a structure close to "core@shell": Pd@PdO. The influence of palladium concentration in the ZnO/Pd composite on the complex of chemoresistive gas-sensitive properties was studied. It is shown that at 250 °C the obtained nanocomposites demonstrate a high (up to 57.8 a.u.) response to 100 ppb–20 ppm acetone, which can be used as a part of supersensitive devices for noninvasive diagnostics of diseases.

Enhanced photo-oxidation of hormones in water using biomass waste-derived hydrochar composites as photocatalysts

This study introduces a green chemistry approach to enhance the catalytic efficiency of metal oxides in photocatalytic reactions using agro-industrial waste. Specifically, rice husks and spent coffee grounds were utilized as carbon sources to produce hydrochars, which served as sustainable supports for ZnO and ZnFe₂O₄ oxides. This method promotes waste minimization and circularity by repurposing agricultural byproducts, reducing the environmental impact of traditional catalyst production. The hydrochars were synthesized through mild hydrothermal carbonization (250 °C for 4 h) and characterized by various physicochemical analyses. Their photocatalytic performance was evaluated using a 20 mg L−1 solution of conjugated estrogens under ultraviolet light (120 W cm−2) for 30 min. Composites with ZnO exhibited a significant increase in oxidation reaction rates due to enhanced porosity and an oxygen-rich structure. Reactive oxygen species (ROS) analysis revealed hydroxyl radicals (OH) as the primary agents, with photocatalytic efficiencies exceeding 83 % after eleven cycles. In toxicity tests, 4 mL of treated solution was applied to 20 Lactuca sativa (lettuce) seeds, kept in the dark at 25 °C for 7 days. Seeds exposed to treated solutions showed improved radicle elongation and germination rates compared to controls, except for those with coffee hydrochar and ZnO. This indicates effective degradation of toxic estrogens without harmful by-products. Overall, this work highlights the advantages of integrating green chemistry principles and circular economy practices, transforming waste into valuable materials to enhance environmental sustainability and catalytic performance.

Evaluating maturity indicators in stingless bee honey: The role of soluble solids over pot condition in harvesting standards

In stingless bee honey (SBH) harvesting, beekeepers often collect honey without accurately assessing its maturation stage, which can impact its physicochemical diversity. This study aimed to evaluate the influence of storage pot condition on SBH maturity by examining honey from open pots (OP) and closed pots (CP). It is commonly assumed that honey in OP is immature and honey in CP is mature. Our physicochemical analyses, including moisture content, soluble solids (°Brix), sugars, and aliphatic organic acids (AOA), tested this hypothesis. The findings revealed that 40 % of SBH samples from OP were mature, challenging the initial assumption. Furthermore, AOA analysis showed that gluconic, acetic, and lactic acids are predominant in SBH, but no direct correlation between AOA concentration and honey maturity was observed. Based on these results, we conclude that OP and CP are not reliable indicators of SBH maturity. This was a preliminary study, providing results that can be further investigated through the validation of the applied methodology and with a larger number of samples. Nevertheless, this study is an important step towards standardizing SBH and assisting producers during the harvest, ultimately optimizing the collection process. To standardize SBH maturity, we recommend using °Brix values, with values above 55 suggesting mature honey with a moisture content below 40 g 100 g⁻¹. Implementing °Brix measurements can improve harvesting practices, enhance honey quality, reduce bee stress, and increase both honey production and market availability.

The fate of a Solanum steroidal alkaloid toxin in the cabbage looper (Trichoplusia ni)

Plants produce complex chemical defenses against herbivores, resulting in the emergence of detoxification strategies in phytophagous insects. While enzymatic detoxification and target site mutagenesis are well-documented, the quantitative contribution of excretion remains less studied. We focus on the cabbage looper (Trichoplusia ni), a generalist herbivore, to elucidate the detoxification of a steroidal alkaloid, solanidine, produced in potato (Solanum tuberosum). Through larval feeding experiments and chemical analysis of metabolites using high-resolution mass spectrometry, we identify solanidine 3-O-β-glucopyranoside and solanidine 3-phosphate as major metabolization products of solanidine. Glycosylation and phosphorylation reactions have not previously been observed in cabbage looper. Modified solanidine derivatives exhibit reduced lipophilicity, preventing passive transport as predicted by physicochemical analyses, and only solanidine was detected in body tissue. In addition, the metabolism of solanidine in a T. ni mutant strain with midgut cadherin protein knocked out was also investigated to examine the potential role of the cadherin, an important receptor for Bt toxins, in steroidal alkaloid detoxification. T. ni cadherin-knockout strain showed lower solanidine conversion (33.9% ± 2.2) and uptake (27.41 ± 0.49 nmol/g) compared to the wild-type strain (51.3% ± 4.1, 33.66 ± 2.48 nmol/g) but similar excretion kinetics. Although solanidine negatively impacted the feeding performance of both strains the cadherin-knockout does not affect the feeding performance. Our study expands the metabolization enzyme repertoire in cabbage loopers, emphasizing the complexity of detoxification mechanisms in generalist herbivores.

Integrating Anaerobic Digestion With Struvite Production for Enhanced Nutrient Recovery, Pathogen Reduction, and Circularity in Manure Management

ABSTRACTIntroductionAnaerobic digestion (AD) is essential for manure management, generating biogas and nutrient‐rich digestate for organic fertilizer. However, improper digestate use can pose environmental risks. Recovering struvite, a magnesium ammonium phosphate (MAP) compound, from digestate provides a sustainable, controlled‐release fertilizer, supporting a circular economy in agriculture.Materials and MethodsThe study employed a two‐stage (liquid–solid) AD process using poultry, dairy, and swine manures, along with wasted corn silage. Digestates were sampled for physicochemical and biogas quality analyses, with feedstocks categorized into D1 and D2, and a composite (D3) formed for struvite characterization. Microbial populations were enumerated on selective media, and struvite mineral content was analysed via argon plasma emission spectrometry.ResultsThe digesters processing feedstock mixtures D1 and D2 achieved specific methane yields of 1.26 L/g CODs fed and 1.49 L/g CODs fed, with cumulative biogas production of 374 and 369 L, respectively, over four 77‐day cycles. The two‐stage AD process significantly reduced antibiotic‐resistant, Enterobacteriaceae and Enterococcus spp. Total ammoniacal nitrogen (TAN) recovery rates were high at 98%–99%, with a consistent struvite crystal mass of 0.67 g/10 mL, indicating the efficiency of this integrated process. The agronomic value of struvite was determined, indicating its potential utility as a fertilizer, and scanning electron microscopy analysis revealed diverse crystal structures, warranting further investigation into their implications for usage and storage.ConclusionThe results suggests that the two‐stage AD process efficiently transforms organic waste into high‐quality biogas, reduces antibiotic‐resistant bacteria, and facilitates nutrient recovery through struvite precipitation. This approach supports co‐digestion of multi‐substrates and promotes circular economy principles, with potassium or sodium phosphate enhancing struvite recovery for sustainable agriculture.

Structural characterization, antioxidant activities and physicochemical properties analysis of a galactose-rich exopolysaccharide produced by Limosilactobacillus fermentum YL-11

Lactobacilli-derived exopolysaccharides (EPSs) are widely regarded as safe and have been employed as potential natural, non-toxic additives and functional agents in food industry. The structural properties of a galactose-rich exopolysaccharide produced by Limosilactobacillus fermentum YL-11 (YL-11 EPS) were investigated with chemical and instrumental analysis. The YL-11 EPS was determined to have a molecular weight (Mw) of 13.2 kDa. Nine different types of glycosidic bonds were identified in YL-11 EPS. The inferred structure of the YL-11 EPS contained the backbone of →2)-β-D-Galp-(1→[3)-β-D-Galp-(1]4 → 3)-β-D-Galp-(1 → 3)-β-D-Galp-(1→, with two side chains, β-D-Galp-(1 → 6)-α-D-Manp-(1 → 6)-[α-D-Glcp-1→2]-α-D-Manp-(1 → 3)-[α-D-Glcp-1→6]-α-D-Glcp-(1 → 3)-[α-D-Glcp-1→6]-α-D-Glcp-(1→ with an acetyl group at the O-3 position of →2,6-α-D-Manp-1→ and α-L-Araf-(1→, which were present at the C6 location of the 1,3-β-D-Galp. The YL-11 EPS showed concentration-dependent antioxidant activities and the DPPH, ABTS, hydroxyl groups and superoxide radicals scavenging rates of 10 mg/mL YL-11 EPS reached 61%, 74%, 35.4% and 40%, respectively. The YL-11 EPS also has a beneficial protective impact on H2O2-injured cells, which pretreatment with YL-11 EPS can substantially decrease ROS levels and enhance SOD enzyme activity. Additionally, the YL-11 EPS exhibited good thickening properties and thermal stability. The EPS's structural information and physicochemical properties provide a basis for studying their structure-function relationships.

Amino Acid–Based Block Copolymer Templates‐Dependent Enhancement of Physicochemical and Electrochemical Characteristics of Mesoporous Carbons

ABSTRACTAmino acid–based block copolymers (BCPs) have distinct features such as secondary structure formation, chirality, amphoteric nature, non‐toxicity, and biodegradability, setting them apart from other BCPs. This suggests that amino acid–based BCPs may exhibit unique self‐assembly behaviors. Despite these advantages, they have not yet been utilized as templates for mesoporous carbons (MCs) synthesis. Here, we investigate, for the first time, the effect of hydrophobicity of two different poly(ethylene glycol) (PEG) conjugated amino acid–based BCP templates (PEG‐poly(β‐benzyl‐l‐aspartate) (PEG‐PBLA) and PEG‐poly(γ‐benzyl‐l‐glutamate) (PEG‐PBLG)) on the construction of MC materials and also their physicochemical and electrochemical characteristics. MCs produced using PEG‐PBLA and PEG‐PBLG as templates are labeled CPBLA and CPBLG, respectively. Utilizing amino acid–based BCP systems enabled achieving MC materials with nearly 1 at% nitrogen doping without external nitrogen dopants. Physicochemical analysis showed CPBLA had a smaller particle size, higher specific surface area, pore size, and hydrophilicity due to increased oxygen and nitrogen contents, as well as a higher defective structure than CPBLG. The higher hydrophilicity of PEG‐PBLA led to the formation of CPBLA MC particles with smaller size and higher specific surface area of 602 m2g−1 and pore size of 7.8 nm. Cyclic voltammetry demonstrated that CPBLA had superior charge‐storing capacity (specific capacitance of 147 F g−1 at 1 mv s−1) than CPBLG, attributed to its better physicochemical properties. These promising findings suggest that amino acid–based BCP systems can serve not only as templates but also as carbon and nitrogen sources, offering potential for high‐performance electrochemical energy storage devices.

The effect of season and agro-ecology on physicochemical properties of cow's raw milk in Central and North-Western Zone of Tigray, Ethiopia

Milk at a normal state has unique physical, chemical, and biological characteristics that are used as quality indicators. The quality of milk is influenced by many factors and the components of milk are very sensitive indicators for milk production practices. Season and agro-ecologies have influences on physicochemical properties of cow milk. Therefore, this research aimed to investigate the effect of season and agro-ecology on the physicochemical properties of cow's raw milk in Central and North-Western Zone of Tigray. The study was conducted in Aksum, Shire-Indaselassie, and Sheraro administrative towns. A two-stage sampling method was used to select both the study area and respondents. All towns in the Central and North-Western zones of Tigray were categorized into three agro-ecologies, and sample towns were purposively selected from each category based on accessibility and milk production potential. Sample respondents were then randomly selected from each agro-ecology. Agro-ecology categorization was based on altitude, temperature, and feeding practices, while seasons were classified according to rainfall and temperature. A total of 210 samples, comprising 35 samples from dairy farms and 35 samples from cafeterias within each agro-ecology, were analyzed to assess the physicochemical properties of cow milk across two different seasons. Physicochemical analysis including determination of fat, crude protein, solids not fat, density, lactose, salt, water added and freezing point was conducted in the laboratory of Mekelle University using Lacto scan, Bulgaria. The overall mean values of milk fat, solids-not-fat (SNF), protein, and lactose were 3.72 ± 0.12 %, 6.72 ± 0.17 %, 2.6 ± 0.08 %, and 3.72 ± 0.09 %, respectively. Specific gravity, salt content, water added, and freezing point exhibited mean values of 1.026 ± 0.001 g/ml, 0.53 ± 0.01 %, 19.33 ± 1.8 %, and −0.42 ± 0.01oC, respectively. Significant differences (p < 0.05) were observed in protein, solids-not-fat, lactose, and salt content among agro-ecologies, seasons, and sources of milk. Correlation analysis revealed positive correlations between specific gravity and solids-not-fat, protein, lactose, and total salt, while negative correlations were observed with water added and freezing point (p ≤ 0.01). In conclusion, the physical and chemical properties of cow's raw milk are influenced by agro-ecology, season, and the source of milk. Notably, milk adulteration, particularly with the addition of water, was more prevalent among cafeteria owners. Given the absence of established milk quality standards in Ethiopian conditions, it is imperative for the government to introduce quality standard measures, rules, and regulations to effectively control milk quality.

Assessement of the physico-chemical quality of Groundwater in the Zagora area, Morocco

Abstract The Zagora region heavily relies on groundwater as a crucial water resource for a range of human activities. Mining activities can have significant impacts on groundwater quality in the areas surrounding mining sites. The primary objective of this study is to analyze and evaluate the effects of pollution on the quality of groundwater in the Zagora area, with a particular focus on mapping and identifying its sources. It is based on the use of Geographic Information Systems (GIS) and the spatial variation of the physico-chemical parameters of the studied water sources. Assessing the physico-chemical quality of groundwater in Zagora, involves evaluating several parameters that can affect its quality. These parameters include temperature (T°C), pH, electrical conductivity (EC), dissolved oxygen (O2), chlorides (Cl−), sulfates (SO4 2−), nitrates (NO3 −), nitrites (NO2 −), ammonium (NH4 +), and orthophosphates (PO4 3−). The results of the physico-chemical analysis have shown that, the electrical conductivity ranged from 501.59 to 3173.92 μs/cm, while sulfate concentrations ranged from 91.87 to 363.02 mg/L. The spatial distribution of the results indicates that the water samples were collected from different locations, and there are variations in the levels of parameters analyzed. Some of the parameters, such as electrical conductivity, and sulfate, have higher levels in some locations compared to others, indicating potential sources of pollution or natural variability in the water composition. The GIS analysis also revealed that the areas with poor water quality were clustered around specific locations, indicating potential sources of contamination. Therefore, further investigation and monitoring of the water sources may be necessary to ensure safe and healthy water consumption.

Evaluation of the quality of treated wastewater from the Fez wastewater treatment plant using wastewater quality index

Abstract The increasing urbanization of Fez, with its daily discharge of wastewater, raises environmental concerns. Therefore, this study assesses the quality of water treated by the Fez wastewater treatment plant, focusing on the summer and autumn seasons, preserving the Oued Sebou from pollution and evaluating treatment efficiency. Key parameters, including on-site measurements and laboratory analyses, indicate average efficiencies of 89.88% (BOD5), 88.41% (COD), and 94.47% (TSS), in compliance with discharge standards. In addition, the results of the principal component analysis (PCA) facilitated understanding of the physico-chemical analyses, underlining the global approach of the study.

Physicochemical and phytochemical analysis of siddha formulation thoothuvalaiyathi chooranam

Aim And Objective: This study aimed to investigate the physicochemical properties and phytochemical composition of siddha formulation thoothuvalaiyathi chooranam. Materials And Methods: Thoothuvalaiyathi Chooranam is one Of the Siddha formulation chosen from Sarabenthira vaithya muraigal kasa swasa sikichai , Page No. 153 which is indicated for Eraippu ,Kasam ,swasam, kabarogangkal. The drug prepared as per the Siddha literature. The drug is subjected to physico chemical analysis such as loss on ignition, loss on drying, water soluble ash etc and phytochemical analysis as per the PLIM guidelines. Results And Conclusion: Phytochemical analysis of this drug was done such as pH (7.10), Loss on ignition (92.10%), Acid Insoluble ash (5.10±0.100), Water soluble extractive (9.30±0.500), Alcohol soluble extractive (8.10±0.200),Loss on drying (0.90±0.500) .The results revealed the presence of various bioactive compounds, including Alkaloids, Carbohydrates, Glycosides, Saponins, Phenols, Triterpenoids, Flavanoids, Proteins and amino acids, Anthocyanin , Tannins.The results indicate the standard quality ,safety and standardization for their use.

Physico-chemical and nutritional characteristics of 12 varieties of cassava (Manihot esculenta Crantz, 1766) grown in the Republic of Guinea

Cassava is a distinctive contributor to food security and a source of income for smallholder farmers. Despite the importance of this agricultural product, very little data is available in Guinea, especially concerning the varieties grown and their nutritional composition. The aim of this research was to study the physico-chemical and nutritional characteristics of 12 varieties of cassava grown in Guinea with a view to selecting varieties that could be used to produce a &lt;&lt;Gari&gt;&gt; of better quality. Cassava tubers were collected in 6 regions in Guinea. The physico-chemical analyses were carried out on the pH and moisture content of the cassava paste from each of the 12 varieties, while the nutritional quality was determined on the basis of the composition of the varieties in carbohydrates, proteins, lipids and minerals. In general, pH values ranged from 6.29 to 7.29 while moisture levels ranged from 51.64% to 58.94%. In addition, all the varieties analysed were those rich in carbohydrates (with levels between 42.36% and 51.17%) but low in fat (values between 0.30% and 3.56%). On the other hand, three varieties, namely Farawoulen, Konko and Samouya, are the richest in protein with values of 8.05%, 5.48% and 4.23% respectively. In short, these cassava varieties produced in Guinea could be used, taking into account their biochemical composition, in future gari production work.

Regulation of yeast &lt;i&gt;RAD9&lt;/i&gt; gene in energy charge, intracellular ROS, and cell cycle arrest in response to DNA damage

In various environmental conditions, eukaryotic cells are exposed to many kinds of exogenous toxic agents as well as to endogenous agents like reactive oxygen species (ROS) generated from oxidative metabolism that can all result in damage to DNA. To cope with these types of damage, yeast cells have evolved a number of mechanisms and specific response systems regulated by key control genes. One of which is RAD9 gene that regulates DNA damage and repair checkpoints, and cell cycle arrest. Thus, a series of methods, e.g. oxygen consumption monitoring, physicochemical analysis, and flow cytometry, were used in the present study to investigate the role of the RAD9 gene by using the BY4742 (wild type) and specific knock-out yeast strains (∆rad9) and elucidate the function of this gene in cellular defense mechanism and metabolic response to DNA damage triggered by methyl methanesulfonate (MMS) treatment. The results indicated that fully functional DNA damage repair and cell cycle checkpoint (RAD9, wild type) significantly enhanced mitochondrial activity and oxygen consumption, reduced intracellular ROS accumulation. Fully functional mitochondria attenuated ROS accumulation, enabled efficient mitochondrial electron transport chain (mtETC) and ATP synthesis, and stabilized cellular energy status. Also, high mitochondrial activity acted as a protective mechanism against oxidative stress. In contrast, deletion of the RAD9 (∆rad9) resulted in high ROS accumulation and damaged to mitochondrial DNA, leading to strong inhibition of mitochondrial activity and oxygen consumption. Furthermore, low mitochondrial activity in cells lacking RAD9 (∆rad9) led to the development of oxidative stress. Subsequently, high ROS accumulation in ∆rad9 cells caused a block of the mtETC, repression of ATP synthesis, fluctuation of cellular energy status, and induction of cell cycle arrest at S and G2/M phases.

Physicochemical, Coliform and Agrochemical Analysis to Evaluate the Water Quality of Coastal Freshwater Wetlands in Tonosí in Panama

Physicochemical, Coliform and Agrochemical Analysis to Evaluate the Water Quality of Coastal Freshwater Wetlands in Tonosí in Panama

Aquatic insect diversity and distribution in Maguri Beel and Khamti Guali Beel wetlands, Upper Brahmaputra Basin, Assam, India

The investigation revealed the presence of a total of 16 species of aquatic insects in Maguri Beel and Khamti Guali Beel, two freshwater ecosystems, in Assam, India. The distribution and diversity of species present in the two wetlands with an account of physico-chemical water analysis and macrophyte distribution are discussed. Variation in the diversity indices of both the wetlands, illustrated key differences in diversity and distribution of aquatic insects in Maguri and Khamti Guali Beel.

Physicochemical and phytochemical analysis of three melon fruit (canary melon, watermelon, and muskmelon) peels, and their valorization in biscuits development

IntroductionMelons are highly sought after worldwide due to their exquisite and delectable taste. However, the peels of these fruits, which are rich in phytochemicals and sustainable components for innovative culinary formulations, are often discarded as waste.MethodsThis study explored the phytochemistry and valorization of three melon fruit, i.e., canary melon (Cucumis melo, var. Fonzy), watermelon (Citrullus lanatus, var. Augusta), and muskmelon (Cucumis melo, var. Cantaloupe), peels in food applications by incorporating into wheat flour biscuits. Peels of fruits were separated, dried and powders were extracted with 70% ethanol.Results and discussionComparing the muskmelon fruit peel to the peels of the other two fruits, the muskmelon fruit peel had the significantly (p &amp;lt; 0.05) high ash (8.36%), fiber (12.06%), fat (1.21%), protein (5.02%), mineral contents (Mg 233, Ca 364, K 1605, Fe 49.72, and Zn 2.39 mg/100 g), total phenolic content (167.49 mg GAE/100 g), total flavonoid content (79.16 mg QE/100 g) and total antioxidant activity (56.92 mg Trolox equivalent/100 g). Results of the antimicrobial experiments of three melon peels showed that extracts from all peels showed enough antimicrobial activities, nearly comparable to the reference drugs (ampicillin and nystatin), which still showed the highest inhibition zones. Among three extracts, highest zone of inhibition against three bacterial species, i.e., Bacillus cereus (12.03 mm), Escherichia coli (10.02 mm), and Streptococcus aureus (18.08 mm), and fungal species, i.e., Candida albicans (8.09 mm), Aspergillus niger (7.06 mm), and Mucor meihi (7.02 mm) was exhibited by muskmelon peel extract. Moisture, ash, fat, fiber, minerals, phenolics, flavonoids, and antioxidant activities of biscuits were increased as a result of incorporating peel flours. Correlation showed TPC and TAA were highly correlated in watermelon (10%; 1). Using principal component analysis, adding 5% canary watermelon peel powder to biscuits improved their healthful and active elements while maintaining sensory features. Baking with 10% muskmelon or watermelon peel powder boosted nutrition and antioxidant activity. After the control, biscuits fortified with 5% melon peel powder exhibited significantly (p &amp;lt; 0.05) high sensory scores. These findings highlight the value of melon peels as sustainable ingredients, promoting waste reduction and enhancing the dietary and functional benefits of food products.

Analytical Techniques for the Authenticity Evaluation of Chokeberry, Blackberry and Raspberry Fruit Wines: Exploring FT-MIR Analysis and Chemometrics

The modern analytical technique of Fourier-transform mid-infrared spectroscopy (FT-MIR) has found its place in routine wine quality control. It allows rapid and nondestructive analysis, with easy sample preparation and without the need for chemical pretreatment or expensive reagents. The objective of this research was to apply these advantages to fruit wines in order to create a tool for the authentication of fruit wines produced from different fruit species (chokeberry, blackberry, and raspberry). The aim of this work was to establish a chemometric model from FT-MIR spectra and to find a “fingerprint” of specific fruit wines, enabling the classification of fruit wines by plant species. Physicochemical analysis of 111 Croatian fruit wine samples (38 liqueur fruit wines and 73 fruit wines) revealed content levels of the following parameters: alcoholic strength (5.0–15.2% vol.), total dry extract (60.4–253.3 g/L), total sugars (1.2–229.9 g/L), pH (3.13–4.98), total acidity (4.2–18.3 g/L) and volatile acidity (0.2–1.5 g/L). For statistical data processing, spectral ranges between 926 and 1450 cm−1 and between 1801 and 2951 cm−1 were used. The first principal component (PC1) explained 70.4% of the observed variation, and the second component (PC2) explained 16.7%, clearly separating chokeberry fruit wines from blackberry and raspberry fruit wines. Soft Independent Modeling Class Analogy (SIMCA) was performed following the development of a PCA model showing that the chokeberry and blackberry wine samples form clearly separated clusters. Key discriminators for classifying chokeberry vs. blackberry wines were identified at 1157, 1304, and 1435 cm−1, demonstrating high discrimination power (DP 26, 17, and 14, respectively). FT-MIR spectroscopy, in combination with chemometric methods, has shown promising potential for the authenticity assessment of fruit wines.