ABSTRACT This study aimed to determine the effect of the intra-session exercise sequence of a concurrent training programme on the components of health-related physical fitness. Twenty-four healthy young adults were allocated into two different groups differing only in the exercise order to conduct an 8-week intra-session concurrent training programme consisting of three sessions of 60–90 minutes (180–270 min/week), with all-out running sprint intervals, back squat, and bench press endurance and resistance exercises (i.e., ET+RT and RT+ET). The 8-week intra-session concurrent training programme overall improved all the components of physical fitness regardless of the exercise sequence. However, ET + RT and RT + ET groups reported moderate and small improvements for squat jump (ET + RT: 3.82 cm [1.11 to 6.53 cm]; RT + ET: 0.31 cm [−1.72 to 2.33 cm]), countermovement jump (ET + RT: 3.76 cm [1.43 to 6.08 cm]; RT + ET: 2.07 cm [−0.03 to 4.17 cm]) and maximum oxygen uptake (ET + RT: 4.75 ml/kg/min [1.14 to 8.35 ml/kg/min]; RT + ET: 1.66 ml/kg/min [−0.89 to 4.21 ml/kg/min]), respectively. Therefore, greater lower-body power and cardiorespiratory fitness gains might be induced following the ET + RT sequence.