Physical Enrichment Research Articles

Efficient Isolation and Purification of High-Quality Arabidopsis thaliana Trichomes.

Trichomes are fine outgrowths on the surface of aerial plant organs which play a role in protecting plants against water loss, UV radiation, and herbivore feeding. Throughout the years, trichomes have become a popular paradigm in biological research. For example, trichomes on rosette leaves of the reference plant Arabidopsis thaliana have been used as a model to investigate cell development, cell differentiation, and, more recently, cell wall biogenesis. State of the art -omics studies on specific cell types or tissues often require physical separation, enrichment, and purification. This, of course, also applies to leaf trichomes, and various methods have thus been proposed to separate trichomes and leaf tissue. Though most of these methods are indeed suitable for trichome isolation, they suffer in part from tedious operating procedures, low yield, poor sample purity, and reduced trichome integrity. We have thus revised a previously reported method for trichome isolation, and report here an efficient and scalable procedure for the isolation and gradient centrifugation-based purification of high-quality A. thaliana trichomes. We describe the preparation of plant material and trichome release, which is based on prolonged gentle agitation of plant seedlings in the presence of a cation-chelating agent that weakens trichome-leaf interactions. We also outline the steps for the subsequent recovery and purification of the isolated crude trichome fraction, which is based on the use of discontinuous sucrose gradient centrifugation. In addition to A. thaliana, we have found that this procedure can be applied to release and enrich glandular and non-glandular trichomes from various species, including Solanum lycopersicum and Nicotiana benthamiana. The resulting purified leaf trichomes can be subjected to different types of bioassays, including histochemistry, biochemical quantification of cell wall monosaccharides, and transcriptomics, as well as proteomic profiling. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Preparation of plant material for release and enrichment of A. thaliana trichomes Basic Protocol 2: Purification of A. thaliana trichomes by density gradient centrifugation.

Physical Enrichment Triggers Brain Plasticity and Influences Blood Plasma Circulating miRNA in Rainbow Trout (Oncorhynchus mykiss).

Simple SummaryOverall, this study has reported that environmental enrichment significantly displayed a series of differentially expressed genes and pathways related to cerebral activity, neural plasticity (neurotrophic markers), neurogenesis, and synaptogenesis, essentially in telencephalon, which may underpin the beneficial effects of a complex environment on rainbow trout’s adaptive behaviors. In addition, environmental enrichment significantly influenced circulating miRNAs (c-miRNAs) profiles of plasma in rainbow trout, which reveals the high potential of c-miRNAs, as physiologically relevant biomarker candidates of fish behavioral plasticity.Physical enrichment is known to improve living conditions of fish held in farming systems and has been shown to promote behavioral plasticity in captive fish. However, the brain’s regulatory-mechanism systems underlying its behavioral effects remain poorly studied. The present study investigated the impact of a three-month exposure to an enriched environment (EE vs. barren environment, BE) on the modulation of brain function in rainbow trout (Oncorhynchus mykiss) juveniles. Using high-throughput RT-qPCR, we assessed mRNA genes related to brain function in several areas of the trout brain. These included markers of cerebral activity and plasticity, neurogenesis, synaptogenesis, or selected neurotransmitters pathways (dopamine, glutamate, GABA, and serotonin). Overall, the fish from EE displayed a series of differentially expressed genes (neurotrophic, neurogenesis, and synaptogenesis markers) essentially localized in the telencephalon, which could underpin the beneficial effects of complexifying the environment on fish brain plasticity. In addition, EE significantly affected blood plasma c-miRNA signatures, as revealed by the upregulation of four c-miRNAs (miR-200b/c-3p, miR-203a-3p, miR-205-1a-5p, miR-218a-5p) in fish blood plasma after 185 days of EE exposure. Overall, we concluded that complexifying the environment through the addition of physical structures that stimulate and encourage fish to explore promotes the trout’s brain function in farming conditions.

Positive effects of bubbles as a feeding predictor on behaviour of farmed rainbow trout

Occupational enrichment emerges as a promising strategy for improving the welfare of farmed animals. This form of enrichment aims to stimulate cognitive abilities of animals by providing them with more opportunities to interact with and control their environment. Predictability of salient daily events, and in particular predictability of feeding, is currently one of the most studied occupational enrichment strategies and can take several forms. In fish, while temporal predictability of feeding has been widely investigated, signalled predictability (based on a signal, such as light or sound) has received little attention. Depending on the type of predictability used and the ecology of the species, the effects on fish welfare often differ. The present study aimed to determine which feeding predictability would be most appropriate for rainbow trout, the main continental farmed fish in Europe, and what the consequences might be for their welfare. We tested four feeding predictability conditions: temporal (based on time of day), signalled (based on bubble diffusion), temporal + signalled (based on time and bubble diffusion), and unpredictable (random feeding times). Behavioural and zootechnical outcomes recorded were swimming activity, aggressive behaviours, burst of accelerations, and jumps, emotional reactivity, and growth. Our results showed that rainbow trout can predict daily feedings relying on time and/or bubbles as predictors as early as two weeks of conditioning, as evidenced by their increased swimming activity before feeding or during feed omission tests, which allowed to reinforce their conditioned response. Temporal predictability alone resulted in an increase in pre-feeding aggressive behaviours, burst of accelerations, and jumps, suggesting that the use of time as the sole predictor of feedings in husbandry practices may be detrimental to fish welfare. Signalled predictability with bubbles alone resulted in fewer pre-feeding agonistic behaviours, burst of accelerations, and jumps than in the temporal predictability condition. The combination of temporal and signalled predictability elicited the highest conditioned response and the level of pre-feeding aggression behaviours, burst of accelerations and jumps tended to be lower than for temporal predictability alone. Interestingly, fish swimming activity during bubble diffusion also revealed that bubbles were highly attractive regardless of the condition. Rainbow trout growth and emotional reactivity were not affected by the predictability condition. We conclude, therefore, that the use of bubbles as a feeding predictor could represent an interesting approach to improve rainbow trout welfare in farms, by acting as both an occupational and physical enrichment.

A Critical Study of Cu2O: Synthesis and Its Application in CO2 Reduction by Photochemical and Electrochemical Approaches

Copper oxide (Cu2O) is a potential material as a catalyst for CO2 reduction. Cu2O nanostructures have many advantages, including interfacial charge separation and transportation, enhanced surface area, quantum efficiency, and feasibility of modification via composite development or integration of the favorable surface functional groups. We cover the current advancements in the synthesis of Cu2O nanomaterials in various morphological dimensions and their photochemical and electrochemical applications, which complies with the physical enrichment of their enhanced activity in every application they are employed in. The scope of fresh designs, namely composites or the hierarchy of copper oxide nanostructures, and various ways to improve CO2 reduction performance are also discussed in this review. Photochemical and electrochemical CO2 transformations have received tremendous attention in the last few years, thanks to the growing interest in renewable sources of energy and green facile chemistry. The current review provides an idea of current photochemical and electrochemical carbon dioxide fixing techniques by using Cu2O-based materials. Carboxylation and carboxylative cyclization, yield valuable chemicals such as carboxylic acids and heterocyclic compounds. Radical ions, which are induced by photo- and electrochemical reactions, as well as other high-energy organic molecules, are regarded as essential mid-products in photochemical and electrochemical reactions with CO2. It has also been claimed that CO2 can be activated to form radical anions.

Harmonisation of welfare indicators for macaques and marmosets used or bred for research

Background: Accurate assessment of the welfare of non-human primates (NHPs) used and bred for scientific purposes is essential for effective implementation of obligations to optimise their well-being, for validation of refinement techniques and novel welfare indicators, and for ensuring the highest quality data is obtained from these animals. Despite the importance of welfare assessment in NHP research, there is little consensus on what should be measured. Greater harmonisation of welfare indicators between facilities would enable greater collaboration and data sharing to address welfare-related questions in the management and use of NHPs. Methods: A Delphi consultation was used to survey attendees of the 2019 NC3Rs Primate Welfare Meeting (73 respondents) to build consensus on which welfare indicators for macaques and marmosets are reliable, valid, and practicable, and how these can be measured. Results: Self-harm behaviour, social enrichment, cage dimensions, body weight, a health monitoring programme, appetite, staff training, and positive reinforcement training were considered valid, reliable, and practicable indicators for macaques (≥70% consensus) within a hypothetical scenario context involving 500 animals. Indicators ranked important for assessing marmoset welfare were body weight, NHP induced and environmentally induced injuries, cage furniture, huddled posture, mortality, blood in excreta, and physical enrichment. Participants working with macaques in infectious disease and breeding identified a greater range of indicators as valid and reliable than did those working in neuroscience and toxicology, where animal-based indicators were considered the most important. The findings for macaques were compared with a previous Delphi consultation, and the expert-defined consensus from the two surveys used to develop a prototype protocol for assessing macaque welfare in research settings. Conclusions: Together the Delphi results and proto-protocol enable those working with research NHPs to more effectively assess the welfare of the animals in their care and to collaborate to advance refinement of NHP management and use.

Enrichment in a Fish Polyculture: Does it Affect Fish Behaviour and Development of Only One Species or Both?

Physical enrichment of structures has been used for the last decades in aquaculture to improve fish production and welfare. Until now, this enrichment has been practiced in fish monoculture but not in fish polyculture. In this study, we developed a polyculture of two freshwater species (pikeperch and sterlet) in recirculated systems (tank of 2.4 m3) with or without physical structures for enrichment. Two types of structures were used: a cover plank on a part of the tank decreasing the light intensity and vertical pipes modifying the water flow. The experiment was conducted in triplicate for a three-month period with juvenile fishes (143 ± 41 g and 27.3 ± 2.2 cm for pikeperch and 133 ± 21 g and 32.8 ± 1.6 cm for sterlet). Behavioural (space occupation and abnormal behaviours) and morphological (total length, final weight, Fulton condition factor, coefficient of variation of the final weight, percentage of biomass gain and specific growth rate) traits were measured. The pikeperch changed their space occupation and showed a preference for low light areas. Sterlet also changed their space occupation: they did not use the cover and occurred mainly in the part of the tank without enrichment. There was no difference for the frequency of abnormal behaviours for pikeperch and sterlet between the two sets (with or without enrichment). There was no statistical difference between the two sets for all the morphological and growth parameters no matter the species and the rearing modality.

Harmonisation of welfare indicators for macaques and marmosets used or bred for research

Background: Accurate assessment of the welfare of non-human primates (NHPs) used and bred for scientific purposes is essential for effective implementation of obligations to optimise their well-being, for validation of refinement techniques and novel welfare indicators, and for ensuring the highest quality data is obtained from these animals. Despite the importance of welfare assessment in NHP research, there is little consensus on what should be measured. Greater harmonisation of welfare indicators between facilities would enable greater collaboration and data sharing to address welfare-related questions in the management and use of NHPs. Methods: A Delphi consultation was used to survey attendees of the 2019 NC3Rs Primate Welfare Meeting (73 respondents) to build consensus on which welfare indicators for macaques and marmosets are reliable, valid, and practicable, and how these can be measured. Results: Self-harm behaviour, social enrichment, cage dimensions, body weight, a health monitoring programme, appetite, staff training, and positive reinforcement training were considered valid, reliable, and practicable indicators for macaques (≥70% consensus) within a hypothetical scenario context involving 500 animals. Indicators ranked important for assessing marmoset welfare were body weight, NHP induced and environmentally induced injuries, cage furniture, huddled posture, mortality, blood in excreta, and physical enrichment. Participants working with macaques in infectious disease and breeding identified a greater range of indicators as valid and reliable than did those working in neuroscience and toxicology, where animal-based indicators were considered the most important. The findings for macaques were compared with a previous Delphi consultation, and the expert-defined consensus from the two surveys used to develop a prototype protocol for assessing macaque welfare in research settings. Conclusions: Together the Delphi results and proto-protocol enable those working with research NHPs to more effectively assess the welfare of the animals in their care and to collaborate to advance refinement of NHP management and use.

Effects of physical enrichment and pair housing before weaning on growth, behaviour and cognitive ability of calves after weaning and regrouping

Housing unweaned calves individually in barren environments negatively affects their growth, cognitive ability, and adaptability to environmental changes in later life. Social housing has been shown to improve those aspects, whereas physical environmental enrichment has rarely been studied in calves. Little is known about whether the combination of both components offers further benefits. Furthermore, curiosity has been considered an intrinsic factor underpinning cognitive performance, which has yet to be determined in calves. The first objective was to compare the effects of providing physical enrichment items and pair housing calves before weaning, and their combination on the weight gain, behaviour and cognitive ability of calves once regrouped after weaning. The second objective was to investigate if calves’ exploratory behaviour in a novel environment can predict their cognitive ability. Forty-eight Holstein calves were allocated to eight groups 2 days after birth. Within each group, two calves were assigned to individual pens and four to two pair pens. One individual pen and one pair pen within each group were provided with brushes, chains, teats, and nets filled with strawberry-scented hay as physical enrichment items. Remaining pens received no additional enrichment items. All calves from a group were introduced to one post-weaning pen when the youngest calf was 9 weeks of age. Calves were weighed on days 1 and 7 in post-weaning pens. They were video-recorded on days 1, 3 and 11 and behavioural data were collected. Spontaneous object recognition tests were conducted within one week after behavioural data collection to assess cognitive ability in terms of how long after exposure calves recognised objects, indicated by differential expression of exploratory behaviour. Physical enrichment items and pair housing had interactions on average daily gain (F1,33 = 5.460, p = 0.026), with calves in physically enriched pair pens showing higher average daily gain than those in non-enriched pair pens and tending to show higher average daily gains than those in physically enriched individual pens. Physically enriched calves expressed more exploratory behaviour and social sniffing than non-enriched calves (F1,111 = 20.691, p < 0.001; F1,111 = 14.433, p < 0.001). Pair housed calves spent more time cross-sucking than individually housed calves (F1,111 = 8.848, p = 0.008). Compared with non-enriched calves, physically enriched calves were more inclined to explore the novel object than the object already presented 15-min ago (χ2 = 3.282, df = 1, p = 0.070). There was no association between exploratory behaviour upon initial introduction to post-weaning pens and performance in object recognition tests. In conclusion, the combination of physical enrichment and pair housing improves calves’ average daily gain after weaning when compared with either component alone. Physical enrichment seemed to improve calves’ memory and adaptability to change, whilst pair housing did not. Calves’ exploratory behaviour in novel environments may not contribute to their cognitive performance.

Giving Ideas Some Legs or Legs Some Ideas? Children's Motor Creativity Is Enhanced by Physical Activity Enrichment: Direct and Mediated Paths.

Approaches to foster motor creativity differ according to whether creative movements are assumed to be enacted creative ideas, or solutions to emerging motor problems that arise from task and environmental constraints. The twofold aim of the current study was to investigate whether (1) an enriched physical education (PE) intervention delivered with a joint constraints-led and cognitive stimulation approach fosters motor creativity, and the responsiveness to the intervention is moderated by baseline motor and cognitive skills and sex; (2) the intervention may benefit motor creativity through gains in motor coordination, executive function, and creative thinking. Ninety-five children, aged 6–9 years, participated in a 6-month group randomized trial with specialist-led enriched PE vs. generalist-led conventional PE. Before and after the intervention, Bertsch’s Test of Motor Creativity, Movement Assessment Battery for Children, Random Number Generation task and Torrance Test of Creative Thinking were administered. Linear mixed models were run accounting for the random effects of data clusters. Multiple mediation analysis was performed to assess whether motor coordination, executive function and creative thinking mediated any improvement of motor creativity. Results showed that (1) specialist-led enriched PE, compared to generalist-led conventional practice, elicited a more pronounced improvement in all motor creativity dimensions (fluency, flexibility, and originality) independently of baseline levels of motor and cognitive skills and sex; and (2) improved motor creativity was partially mediated by improved motor coordination and, as regards motor flexibility, also by improved inhibitory ability. In conclusion, enriching PE with tailored manipulations of constraints and variability may enhance the ability to create multiple and original task-pertinent movements both directly and through indirect paths. The results are discussed extending to motor creativity a theoretical framework that distinguishes different creativity modes. The intervention may have fostered the generation of creative movements directly through the exposure to variation in constraints, activating the sensorimotor ‘flow’ mode of creativity that bypasses higher-order cognition, but also indirectly through a systematic and conscious convergence on solutions, activating the ‘deliberate’ mode of creativity that relies on inhibition to reject common or task-inappropriate movement categories.

Harmonisation of welfare indicators for macaques and marmosets used or bred for research.

Background: Accurate assessment of the welfare of non-human primates (NHPs) used and bred for scientific purposes is essential for effective implementation of obligations to optimise their well-being, for validation of refinement techniques and novel welfare indicators, and for ensuring the highest quality data is obtained from these animals. Despite the importance of welfare assessment in NHP research, there is little consensus on what should be measured. Greater harmonisation of welfare indicators between facilities would enable greater collaboration and data sharing to address welfare-related questions in the management and use of NHPs. Methods: A Delphi consultation was used to survey attendees of the 2019 NC3Rs Primate Welfare Meeting (73 respondents) to build consensus on which welfare indicators for macaques and marmosets are reliable, valid, and practicable, and how these can be measured. Results: Self-harm behaviour, social enrichment, cage dimensions, body weight, a health monitoring programme, appetite, staff training, and positive reinforcement training were considered valid, reliable, and practicable indicators for macaques (≥70% consensus) within a hypothetical scenario context involving 500 animals. Indicators ranked important for assessing marmoset welfare were body weight, NHP induced and environmentally induced injuries, cage furniture, huddled posture, mortality, blood in excreta, and physical enrichment. Participants working with macaques in infectious disease and breeding identified a greater range of indicators as valid and reliable than did those working in neuroscience and toxicology, where animal-based indicators were considered the most important. The findings for macaques were compared with a previous Delphi consultation, and the expert-defined consensus from the two surveys used to develop a prototype protocol for assessing macaque welfare in research settings. Conclusions: Together the Delphi results and proto-protocol enable those working with research NHPs to more effectively assess the welfare of the animals in their care and to collaborate to advance refinement of NHP management and use.

Colored skins and vibrant hybrids: Manipulating visual perceptions of depth and form in double‐curved architectural surfaces through informed use of color, transparency and light

AbstractThe past decades of research on color and light yielded vast knowledge supporting their informed use in architectural design. While there currently exists a rich body of knowledge and methods geared to affect the perception of depth and form in tiled, opaque architectural surfaces, not many such methods have been developed for double‐curved, transparent, in‐mass colored surfaces. The perception of depth and form in these surfaces relies on a complex blend of parameters, such as color combinations, illumination source, angle of viewing, location of shadows and reflections, material thickness and grade of transparency. To determine the visual effects caused by some of these parameters, experiments based on visual observations were carried out involving handcrafted, in‐mass colored, undulant architectural surfaces. The insights from the experiments then served to develop four color strategies for architectural surface design harnessing the discovered effects in diverse ways. Through this, the study has sought first to observe and understand the effects of color and light in perceiving undulant surfaces, and second to highlight the potentials of harnessing these effects in the design of expressive architectural elements. The main insight from the study is that informed and deliberate application of color and light yields a wide range of potentially interesting perceptual effects in double‐curved architectural surfaces, such as spatial filtering, gradient screening, vibrant massing and animate reshaping. Such effects, applied in an architectural context, can help to fulfill the demand for physical environmental enrichment in the digital era.

An evaluation of the role of ‘biological evidence’ in zoo and aquarium enrichment practices

AbstractEvidence-based approaches are key to advancing all areas of zoo and aquarium practice. Output from empirical study must be disseminated to those within the industry so that results can support changes to husbandry and management for individual species. Information on enrichment techniques is published in a range of sources, including papers in the peer-reviewed and ‘grey literature’ (ie professional but non-reviewed publications). To investigate how evidence is implemented into enrichment practices, we sampled all enrichment studies identified in one online repository of peer-reviewed papers and two grey literature publications across an eleven-year period. We recorded whether the enrichment was supported with biological evidence (whether it was developed using published enrichment-focused research for that species and/or with the species’ ecology and behaviour information) alongside analysis of the type of enrichment used and the chosen study species. Enrichment articles were more likely to be supported by biological evidence in peer-reviewed than grey literature. Taxonomic differences in the use of evidence were noted; for example, enrichment provided to carnivores and parrots was more likely to be supported with biological evidence compared to that used for penguins. Of the five enrichment types, nutritional enrichment was most often based on biological evidence. Multi-category and physical enrichment types were more common across all literature sources whereas social enrichment was less common, suggesting barriers to implementation of all enrichment types in zoological facilities. Our research suggests that zoo and aquarium professionals are considering species-specific welfare needs by ensuring that enrichment protocols are supported by biological evidence. However, opportunities to diversify the enrichment types being offered and species being researched are identified.

Effects of Wolf Spiders' Captive Environment on Their Locomotor and Exploratory Behaviours.

Simple SummarySince the 1960s, abuses of domestic and of wild animals that have been tamed or are held in captivity have been legally prohibited and laws ensure their well-being. Many scientific investigations carried out in this context recommend ways to adapt farming and thus to avoid physical and/or psychological suffering. Evaluations of animals’ welfare in captivity entail the need to understand in detail the fundamental behaviours of the focus species and to know the degree of their variation to be able to establish objective bases that can ensure breeding conditions that respect the animals’ welfare. Current laws do not apply to invertebrate animals (such as insects or spiders) and consideration of the welfare of these animals in captivity is neglected. Here, I compared the behaviour of wild adult spiders just after collection and that of adult spiders hatched and bred in the laboratory. My results show that captivity induced rapid changes of wild spiders’ behaviour once in captivity. Therefore, it is important to establish the best breeding conditions for the needs of both invertebrate and vertebrate animals in order to promote their well-being.Here I detail the effects of the abiotic/captive environment of an adult wandering spider, Pardosa saltans (Lycosidae) on its behaviour. These studies focused on spiders collected as adults in their natural environment and spiders developed in the laboratory under controlled conditions. Wild-caught spiders were tested either immediately after capture or after being housed for 15 days post-collection. Laboratory reared spiders were kept in different environments: small or large space combined with the presence or absence of litter. Two tests evaluated by sex show the influence of these rearing conditions: an open-field test and a radial-arm maze test. The results show that wild caught spiders of both sexes tested immediately after capture weighed significantly less and were significantly more active than spiders housed in the laboratory for 15 days and spiders reared in the laboratory. Laboratory conditions induced a positive impact on body mass and negative impact on behaviour activities. The locomotor and exploratory activities of spiders of both sexes kept in container without substrate showed lower. My results suggest that the physical enrichment of the environment can reduce these negative effects for females, but not for males that seem to be more affected by being reared under controlled conditions.

Regional Differences in BDNF Expression and Behavior as a Function of Sex and Enrichment Type: Oxytocin Matters.

The early environment is critical to brain development, but the relative contribution of physical versus social stimulation is unclear. Here, we investigated in male and female rats the response to early physical and social environmental enrichment in relation to oxytocin (OT) and brain-derived neurotrophic factor (BDNF) expression. The findings show that males and females respond differently to prolonged sensorimotor stimulation from postnatal days 21-110 in terms of functional, structural, and molecular changes in the hippocampus versus medial prefrontal cortex (mPFC). Physical enrichment promoted motor and cognitive functions and hippocampal BDNF mRNA and protein expression in both sexes. Combined physical and social enrichment, however, promoted functional and structural gain in females. These changes were accompanied by elevated plasma oxytocin (OT) levels and BDNF mRNA expression in the mPFC, while the hippocampus was not affected. Administration of an OT antagonist in females blocked the beneficial effects of enrichment and led to reduced cortical BDNF signaling. These findings suggest that an OT-based mechanism selectively stimulates a region-specific BDNF response which is dependent on the type of experience.

Relationships between owner and household characteristics and enrichment and cat behaviour

Owners may enhance their cats’ welfare by social enrichment (e.g. positive human-animal interactions), and physical enrichment (e.g. play objects). The purpose of this study was to investigate associations between owner characteristics (e.g. attitudes, attachment), household characteristics (the keeping of one or more cats) and owner behaviours enriching their cats’ lives. Another aim was to use the Theory of Planned Behaviour (TPB, using question sets targeting attitudes towards behaviours directed/activities provided to cats, normative and control beliefs) to identify predictors of owner behavior, represented by duration of a play session, number of toys constantly available/cat and frequency of outdoor access. Finally, we aimed to investigate associations between owner and cat behaviours. To this end, an online survey was conducted among cat owners. Questions assessing general attitudes, beliefs about cats’ needs, attachment, frequencies of owner-cat interactions, access to play opportunities and cat behaviour (play, unwanted behaviours) were summarised to components after principal component analyses. Owner attitudes and attachment systematically correlated with frequencies of human-cat interactions (e.g. tactile and non-tactile) and access to various play objects (p < 0.05). The general attitude that cats are ‘Beneficial’ and the TPB attitude ‘Important to play with cats’ were significant predictors of the duration of a play session (β = 0.15/0.14), accounting for 8% of the variance. Owner age, the husbandry decision to keep one or more cats and the TPB attitude ‘Important to offer different toys’ significantly predicted the number of toys constantly available/cat (β = −0.12/−0.47/0.45), accounting for 36% of the variance. Predictors of the frequency of outdoor access were the TPB attitude ‘Important to offer outdoor access’ and the control belief that the outdoors were too dangerous (β = 0.23/−0.60), accounting for 62% of the variance. According to structural models, an effect of attachment on owner behaviour is mediated by attitudes. Owner behaviours systematically correlated with cat play (p < 0.05) but not problem behaviours. The overall results suggest clearly identifiable relationships between attitudes, owner and animal behaviour. They provide insight into attitudes and owner behaviours to target when designing interventions to influence cat owner behaviour. Since positive owner–cat interactions were associated with cat play behaviour, a potential indicator of enhanced welfare, this study underlines the important role of the owner for cat welfare.

Effects of simple shelters on growth performance and welfare of rainbow trout juveniles

Environmental enrichment is a promising strategy to improve the welfare of fish in captivity. However, the utilization of enrichment in aquaculture is still infrequent, maybe because there is a paucity of knowledge about how its effects depend on factors such as fish species, developmental stage, social environment or the type and extent of enrichment. In this study, we evaluated the effects of physical enrichment on the welfare of rainbow trout juveniles, by exposing them to simple plastic screen shelters. Juveniles of approx. 15 g were introduced to two types of submerged shelters: full screens (Full) or partial screens (Semi), and fish welfare was assessed and compared to a control group (without shelters) by evaluating fish growth and condition, extent of external lesions, and the neuroendocrine responses to acute and repeated stress. During the eleven-week experimental period, the fish in the sheltered units gradually developed a clear shelter-seeking behavior when exposed to external disturbance. Fish growth, condition factor and mortality were not affected by shelter presence. The presence of full shelters had a modest protective effect on fin damage: both pectoral fin- and total fin damage scores were reduced (> 10%) in this group with respect to the control group; the percentage of fish with severe damage in the pectoral fin was reduced in the Full group with respect to the Control (63% vs 82%). Partial shelters had no significant effect on fin damage scores, when compared to the control group. The presence of shelters did not affect the general level of stress upon standardized acute or repeated stressors. However, fish used to the presence of shelters showed a more intense startling response when exposed to stressors that forced them to abandon the shelter protection. Altogether, this study shows potential for shelters to be used as a welfare-promoting strategy in trout farming, but further research is needed to optimize the shelter type and design and the proper timing for its application.

Possibilities of reducing fearfulness and stress in horses by prophylactic methods and behavioural therapies

The aim of this review was to present the possibilities of minimizing fearfulness and stress in horses by various prophylactic and therapeutic methods. The teaching techniques mentioned in the article (imprint training, habituation, desensitization, counterconditioning) can be successfully used not only during the early development of human-horse interaction, but also during the therapeutic treatment of undesired and stereotypic behaviours. Horses’ activity in the paddock and/or stable can be increased by different types of environmental enrichment (feeding, social, sensory, occupational and physical enrichment). Such environmental variety has a positive influence on equine welfare and can prevent the development of problematic behaviour. Animals living in a highly enriched environment and properly interacting with humans react with lower intensity to environmental stimuli. All these factors have a positive effect on the quality of the human-horse relationship, making the everyday maintenance and use of horses easier and safer.

The Protein-Protein Interaction Network of Hereditary Parkinsonism Genes Is a Hierarchical Scale-Free Network.

PurposeHereditary parkinsonism genes consist of causative genes of familial Parkinson’s disease (PD) with a locus symbol prefix (PARK genes) and hereditary atypical parkinsonian disorders that present atypical features and limited responsiveness to levodopa (non-PARK genes). Although studies have shown that hereditary parkinsonism genes are related to idiopathic PD at the phenotypic, gene expression, and genomic levels, no study has systematically investigated connectivity among the proteins encoded by these genes at the protein-protein interaction (PPI) level.Materials and MethodsTopological measurements and physical interaction enrichment were performed to assess PPI networks constructed using some or all the proteins encoded by hereditary parkinsonism genes (n=96), which were curated using the Online Mendelian Inheritance in Man database and literature.ResultsNon-PARK and PARK genes were involved in common functional modules related to autophagy, mitochondrial or lysosomal organization, catecholamine metabolic process, chemical synapse transmission, response to oxidative stress, neuronal apoptosis, regulation of cellular protein catabolic process, and vesicle-mediated transport in synapse. The hereditary parkinsonism proteins formed a single large network comprising 51 nodes, 83 edges, and three PPI pairs. The probability of degree distribution followed a power-law scaling behavior, with a degree exponent of 1.24 and a correlation coefficient of 0.92. LRRK2 was identified as a hub gene with the highest degree of betweenness centrality; its physical interaction enrichment score was 1.28, which was highly significant.ConclusionBoth PARK and non-PARK genes show high connectivity at the PPI and biological functional levels.

Influence of environmental enrichment on the rehabilitation of white-eyed parakeet (Psittacara leucophthalmus)

The use of sensory, physical, cognitive, and alimentary stimuli are varieties of environmental enrichment used to minimize stress caused by the monotonous captive environment. The objective of this study was to verify the impact of environmental enrichment in escape-related behavioral stereotypies. Thirty birds of the species Psittacara leucophthalmus were observed from March to September 2016, received at the Wild Animal Recovery and Screening Center of the São Paulo State University (UNESP), School of the Veterinary Medicine campus Araçatuba. The methodology used for the behavioral observations was the focal animal with observation through filming were made by 18 hours per bird for three consecutive days, while physical, cognitive, food, and sensory enrichment methods were applied, and the assessment was carried out before, during, and after the application of environmental enrichment. The data were analyzed by the Shapiro-Wilk test for normality and by the Friedman test, which showed no significant difference (p &gt;0.05) before, during, and after environmental enrichment. Despite the statistical analyses, the perception of the bird’s welfare improving was visually clear.

Life in captivity: future research in physical enrichment for fish.

Life in captivity: future research in physical enrichment for fish.