Physical hydrogels produced by chitosan low (LCh) and medium molecular weight (MCh) were loaded with TiO2 nanoparticles (TiO2NPs) and TiO2 nanoparticles loaded with resveratrol extract (Polygonum cuspidatum) (TiO2NPsRes). Equilibrium water content, EDX mapping analysis, and hemocompatibility of chitosan hydrogels were evaluated. Chitosan hydrogels preserved their porous structure, displaying mean pore size between 111.7 and 139 µm. The equilibrium water content of the physical chitosan hydrogels reached values of 24 times their mass in distilled water, decreasing with the addition of TiO2. The EDX analysis corroborated that chitosan hydrogels displayed high titanium concentration (8.8–9.5 % w/w), while resveratrol extract reduced the load of titanium in the hydrogels (0.2 and 2.1 % w/w). The hemocompatibility of hydrogels was suitable with hemolytic values as low as 2.1 and 3.1 %. Physical chitosan hydrogels loaded with TiO2 and TiO2Res can be candidates for biomaterials in biomedical applications.