Physical Barrier Research Articles

An HD-Zip III transcription factor, BjPHVa, negatively regulates non-glandular trichome formation in Brassica juncea.

Trichomes are specialized structures derived from epidermal cells. Non-glandular trichomes primarily protect plants from herbivores and intense ultraviolet radiation by acting as a physical barrier. Recent research has highlighted the importance of homeodomain leucine zipper (HD-Zip) IV transcription factors (TFs) in promoting trichome development. In this study, an HD-Zip III TF called PHAVOLUTA (BjPHVa) was identified as a negative regulator of non-glandular trichome initiation in Brassica juncea. Genome editing of BjPHVa resulted in a significant increase in trichome number in B. juncea. Co-expression networks revealed a strong association between trichome development and the HD-Zip family, which was supported by transcriptomic analysis findings. An R2R3-MYB TF, BjGL1a, a key regulator of trichome development, was found to be associated with BjPHVa-regulated trichome development. Knockdown of BjGL1a expression resulted in reduced trichome number in B. juncea. BjPHVa was observed to interact directly with BjGL1a while binding to the BjGL1a promoter, resulting in the inhibition of BjGL1a transcription. These results provide new insights into the identification of regulators involved in trichome development and offer new opportunities to enhance resistance to predicted stresses through genome editing targeting PHVa within Brassicaceae.

A biodegradable shape memory polyurethane film as a postoperative anti-adhesion barrier for minimally invasive surgery

Postoperative adhesions commonly form in various tissues, resulting in serious implications and an increased risk of secondary surgery. The application of anti-adhesion films as physical barriers has proven effective in reducing adhesion incidence and severity. However, existing anti-adhesion films require manual deployment during minimally invasive surgery, posing inconvenience and possibility of further injury. To address these limitations, we have developed an intelligent anti-adhesion film based on shape memory polyurethane. In this work, a linear shape memory polyurethane (ISO2-PU), incorporating hexamethylene isocyanate and isosorbitol as hard segments and poly(D, L-lactic acid) macrodiol as soft segments, was fabricated into an anti-adhesion film. The favorable shape memory effect of the ISO2-PU film ensures its convenient delivery and automatic unfolding, as revealed by a simulation experiment for endoscopic surgical implantation. Furthermore, the glass transition temperature (Tg) close to body temperature endows the ISO2-PU film with good mechanical compliance, thus ensuring a reliable fit with the wounded tissue to avoid undesired folding. Finally, in vivo experiments using a rat cecal abdominal wall injury model demonstrated that the combination of reliable fit, appropriate degradation rate, and good cytocompatibility promises the ISO2-PU film with high anti-adhesion efficacy. This work validates the concept of shape memory anti-adhesion barrier and expands future directions for advanced anti-adhesion biomaterials. Statement of significancePostoperative adhesions are a common complication that occurs widely after various surgeries. This work developed an intelligent anti-adhesion film based on a linear shape memory polyurethane (ISO2-PU). This film is featured with remarkable shape memory effect and mechanical compliance at body temperature, appropriate degradability, and good cytocompatibility. These merits ensure convenient delivery and smart unfolding of ISO2-PU film during minimally invasive surgery and favorable postoperative anti-adhesion efficacy. The results validate the concept of shape memory anti-adhesion barrier and paves a way for designing next-generation anti-adhesion biomaterials.

Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri: intraspecific and interspecific patterns

Lactobacillus rhamnosus (Rh) and Lactobacillus reuteri (Re) are well-known probiotic species in inflammatory bowel disease (IBD) research. The variations between these species’ efficacy against colitis, and their model of action in this regard, are intriguing and enable treatment to be individually tailored to patients. In this study, four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced. The anti-colitis activities of both strains involved various aspects of intestinal immune, physical, chemical, and biological barrier function. Strikingly, the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration. Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis. Nevertheless, Re strains were more effective than Rh strains in inhibiting production of inflammatory factors; promoting production of intestinal mucus, antimicrobial peptides, and tight junction proteins; and supporting the stem cell compartment. This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains. In addition, the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism- and prophage-related genes, respectively. Taken together, the results of this study could assist researchers in developing effective therapies for IBD.

Equity And Inclusion For Access To The Brazilian Unified Health System (SUS)

The concept of equity in the Unified Health System (SUS) is crucial for ensuring a fair distribution of resources and health opportunities, taking into account the specific needs of different population groups. This principle aims not only to ensure universal access to health services but also to adjust these services to address socioeconomic, geographic, and cultural inequalities. Equity in SUS involves providing additional support to the most vulnerable populations, such as individuals with low income, those living in remote areas, and groups facing cultural and language barriers. Significant challenges include regional disparities, with inadequate health infrastructure in rural and peripheral areas, and socioeconomic inequalities that hinder access to care due to indirect costs and lack of information. To address these challenges, SUS must adopt multifaceted strategies, including increased funding and resources for underserved areas, public policies to reduce socioeconomic inequalities, and investments in transportation and health education. Inclusion in SUS is equally fundamental, requiring the removal of physical and social barriers, the promotion of a culture of respect and cultural competence among health professionals, and the active participation of diverse groups in health policy formulation. Additional strategies involve interinstitutional collaboration, community engagement, and the collection and analysis of disaggregated data to inform targeted policies. These approaches aim to create a fairer and more inclusive health system capable of meeting the needs of the entire Brazilian population

Challenges And Strategies In Healthcare Confronting Population Aging In Brazil

The concept of equity in the Unified Health System (SUS) is essential for ensuring a fair distribution of resources and health opportunities, taking into account the specific needs of different population groups. Equity goes beyond universal access to healthcare services, encompassing the adaptation of these services to address socioeconomic, geographic, and cultural inequalities. In SUS, this means providing additional support to vulnerable populations, such as low-income individuals, residents of remote areas, and groups with cultural and linguistic barriers. Key challenges include regional disparities, such as inadequate healthcare infrastructure in rural and peripheral areas, and socioeconomic inequalities that hinder access due to indirect costs and lack of information. To overcome these challenges, SUS should adopt comprehensive strategies, such as increasing funding and resources for underserved areas, implementing public policies that address socioeconomic inequalities, and investing in transportation and health education. Inclusion is equally crucial, requiring the removal of physical and social barriers, the promotion of a culture of respect and cultural competence among healthcare professionals, and the active participation of diverse groups in policy formulation. Furthermore, promoting interinstitutional collaboration, community participation, and the collection and analysis of disaggregated data to guide targeted policies is important. These strategies aim to create a fairer and more inclusive health system, capable of adequately serving the entire Brazilian population

Advanced Oral Drug Delivery Systems for Combating and Preventing Paediatric Periodontal Disease

: The oral route is the most common route of administration of drugs. Over 90% of all the available marketed pharmaceutical products are oral formulations. Oral drugs are used in different courses of treatment including the prevention of tooth decay. Tooth decay is the permanent damage of the enamel which leads to the formation of cavities. It can be prevented with good oral hygiene and enough fluorides in the body. Fluorides can be administered both topically (toothpastes) and systemically (supplements). Fluoride supplements fall under oral drug delivery systems. They come in the form of tablets, lozenges, and liquids. However, challenges are faced when it comes to oral drug delivery in children. The development of paediatric drugs is a difficult undertaking since many pharmaceutically active compounds have low water solubility, instability, or an unpleasant taste. Children are unable to tolerate bitter or unpleasant- tasting formulations, as well as huge pills and capsules. Due to various biological, biochemical, and physical barriers faced by oral drug delivery systems, new approaches have been developed to address these challenges such as the application of nanotechnology in drug development. Jellies for oral administration on the other hand are a new approach for the delivery of drugs with bitter tastes as well as for age groups such as children and elders. They are clear, translucent, or non-greasy semisolid products that can be used both externally and internally. In-depth, aspects of these factors will be discussed in this review paper including oral dosage forms for paediatrics, tooth decay and its pathogenesis, preventive measures and setbacks of each measure as well as the future perspectives.

Influence of Anastrepha host fruit size over parasitism by Diachasmimorpha longicaudata in open-field augmentative releases

ABSTRACT Fruit size can act as a physical barrier providing refuge for larvae of pest Anastrepha species against parasitoid attack. Therefore, the aim of this study was to evaluate the influence of fruit size in six fruit species infested by Anastrepha larvae on the parasitism of Diachasmimorpha longicaudata after its augmentative release. About 1500 parasitoids were weekly released in disturbed wild vegetation patches. Fruit sampling was performed at the Coastal Lowlands of the Soconusco Region, southeastern Mexico, between June 2021 and October 2022. Overall, 310 ripe fruits belonging to grapefruit, creole mamey (large-size fruits), sweet orange, creole mango, wild guava (medium-size fruits), and wild hog plum (small-size fruit) were collected. The length, frontal width, lateral width and pulp thickness of all sampled fruits were measured. Such physical characteristics are indicators of the fruit size. Fruit collection was performed 48 h after release. Results indicated an inverse correlation between fruit size and parasitism, with a significant r 2 value higher than 75%. Host larvae in all fruits were parasitised only by D. longicaudata. The highest mean parasitism, which exceeded 90%, was recorded on hog plum and the lowest, below 20%, on Citrus spp. and mamey. Results corroborated the influence of host fruit size on parasitism levels by D. longicaudata regardless of its large-scale, open-field releases. Augmentative releases of D. longicaudata can be focused on wilderness areas with prevalence of medium-size to small-size Anastrepha host fruits. This would encourage long-term sustainable conservation of released parasitoid populations.

Emulsifying properties of egg proteins: Influencing factors, modification techniques, and applications.

As an essential food ingredient with good nutritional and functional properties and health benefits, eggs are widely utilized in food formulations. In particular, egg proteins have good emulsification properties and can be commonly used in various food products, such as mayonnaise and baked goods. Egg protein particles can act as stabilizers for Pickering emulsions because they can effectively adsorb at the oil-water interface, reduce interfacial tension, and form a stable physical barrier. Due to their emulsifying properties, biocompatibility, controlled release capabilities, and ability to protect bioactive substances, egg proteins have become ideal carriers for encapsulating and delivering functional substances. The focus of this review is to summarize current advances in using egg proteins as emulsifiers. The effects of influencing factors (temperature, pH, and ionic strength) and various modification methods (physical, chemical, and biological modification) on the emulsifying properties of egg proteins are discussed. In addition, the application of egg proteins as emulsifiers in food products is presented. Through in-depth research on the emulsifying properties of egg proteins, the optimization of their applications in food, biomedical, and other fields can be achieved.

Healthcare Provider's Preferences on Open Versus Restricted Visiting Hours in Surgical Intensive Care Unit.

The increasing consideration of open visiting hours in intensive care units (ICUs) has presented implementation challenges. Recognizing the invaluable insights of healthcare providers, this study aimed to assess their perspectives on the potential impact of altering ICU visiting policies on patient care. In a single-institution, cross-sectional study, a five-point Likert survey was administered to healthcare providers working in a surgical ICU to assess their perceptions of open and restricted visiting hours. Descriptive statistics were used to analyze the responses. Ninety-three healthcare providers participated, including nurses (53%), resident physicians (23%), and other healthcare professionals. The majority (81%) did not believe that open ICU visiting hours would enhance patient-centered care. Providers expressed concerns that open visitation could lead to increased provider stress (89%), physical barriers to patient access (80%), disruption in workflow (90%), and provider burnout (76%). Conversely, they perceived that restricted visitation would preserve patient privacy (87%), promote better healing for patients (64%), reduce provider stress (81%), and improve workflow (95%). Nurses were more likely than resident physicians to perceive that open ICU hours would alleviate familial stress (p=0.022). Additionally, both nurses and other healthcare providers recognized the potential for open ICU hours to improve communication between patient families and healthcare teams (p<0.05). Notably, there were no significant differences among providers in perceptions of the impact of open versus restricted visitation on provider stress, workflow, or other factors (p>0.05). This survey reveals a negative perception of open ICU visiting hours among healthcare providers in a surgical ICU, with concerns that such a change could increase stress for both patients and providers. These findings underscore the potential challenges and negative impacts of implementing open visiting hours in this setting, emphasizing the need for careful consideration and further research to inform policy decisions and visitation practices.

Interventions based on alternative and sustainable strategies for postharvest control of anthracnose and maintain quality in tropical fruits.

Colletotrichum spp. is a phytopathogen causing anthracnose in a variety of tropical fruits. Strategies used to control postharvest diseases in tropical fruits typically rely on the use of synthetic fungicides, which have stimulated the emergence of resistant pathogens. Safer alternative strategies to control anthracnose in tropical fruits have been described in the literature. This review presents and discusses the main innovative interventions concerning the application of sustainable alternative strategies in the postharvest control of pathogenic Colletotrichum species in tropical fruits, with a particular emphasis on the studies published in the last 5 years. The available studies have shown the use of various methods, including physical barriers, natural antimicrobials, and biological control with antagonistic microorganisms, to reduce anthracnose lesion severity and incidence in tropical fruits. The available literature showed high inhibitory activity in vitro, reduced anthracnose incidence and lesion diameter, and total disease inhibition in tropical fruits. Most studies focused on the inhibition of Colletotrichum gloeosporioides on avocado, papaya, and mango, as well as of Colletotrichum musae on banana; however, the inhibition of other Colletotrichum species was also demonstrated. The application of emerging sustainable alternative methods, including natural antimicrobial substances, also stimulated the induction of defense systems in tropical fruits, including enzymatic activity, such as polyphenol oxidase, peroxidase, and phenylalanine ammonia-lyase. The retrieved data helped to understand the current state of the research field and reveal new perspectives on developing efficient and sustainable intervention strategies to control pathogenic Colletotrichum species and anthracnose development in tropical fruits.

The Art of Nano-CAR T Cells in Ovarian Cancer Management

The combination of nanotechnology and CAR T-cell therapeutics heralds a paradigm shift in the management of ovarian cancer, a disease characterized by substantial obstacles such as antigenic heterogeneity, an immunosuppressive tumor microenvironment, and physical barriers to treatment efficacy. This paper examines these obstacles critically and proposes innovative nano-engineered solutions, such as the development of nano-CAR T cells and nanoparticle-mediated delivery of CAR genes and immunomodulatory agents, as strategic approaches to improve CAR T-cell delivery, survival, and function within the hostile tumor microenvironment. The convergence of these two disciplines represents a promising strategy for overcoming these obstacles and optimizing the therapeutic potential of CAR T cells in solid malignancies. To translate this promising approach into a viable clinical reality in the fight against ovarian cancer, however, additional scientific research and rigorous clinical trials are required to ensure safety, optimize the design, and establish standardized production and application protocols.

Generation and content validation of physical and vocational rehabilitation in paraplegics: An interview guide

BACKGROUND: As the mainstay treatment for paraplegics is rehabilitation, patient satisfaction and experience are important predictors of the care being provided. To understand whether the current arrangements of rehabilitation services are sufficient to help an individual with paraplegia return to a productive life. Patient-centered interview guides to explore their rehabilitation experience are lacking, which calls for a generation of the same. OBJECTIVE: To generate and validate an interview guide (PVR-PIG), which aims at understanding and exploring the experience of physical and vocational rehabilitation for individuals with paraplegia. METHODS: After an extensive literature search, an initial 27-item interview guide was formulated under three domains: general experience and expectations from rehabilitation care; physical rehabilitation experience, barriers, and facilitators; and vocational rehabilitation. Later, it was sent for content validation to a multidisciplinary expert panel with prior experience in spinal cord injury (SCI) management. RESULTS: After suggestions were received from nine experts with an average of 14 years of experience in dealing with SCI, a 15-item interview guide was finalized, with a scale-level and item-level content validity index of 0.897 and 0.78–1, respectively. CONCLUSION: The generated interview guide (PVR-PIG) aimed to explore physical and vocational rehabilitation experiences in paraplegics was found to be valid.

Participation of Disabled Women in the Indian Parliament since Independence

This study looks at how women with disabilities have been part of the Indian Parliament since India became independent. We found that very few women with disabilities have become members of parliament. Less than 1 out of every 100 women MPs has a disability. This is despite new laws meant to help people with disabilities. We looked at how things have changed over time, what laws exist, and how India compares to other countries. The few women with disabilities who became MPs have done important work. They have spoken up for disability rights and helped change how people think. But many things still make it hard for women with disabilities to enter politics. These include physical barriers and unfair treatment. We suggest ways to improve this, like setting aside seats for women with disabilities and making politics more accessible. Having more women with disabilities in parliament is important to make sure everyone in India is truly represented.

“No Foreigners”: Migrants’ Experiences of Urban Bordering in the City of Zagreb

ABSTRACT This article examines the interplay between non-European migration in the Balkans and the securitization of the European Union’s borders, highlighting the diffusion of bordering practices into urban spaces. Drawing on ethnographic research conducted in Zagreb, Croatia, we explore how migrants’ encounters with urban bordering contribute to their experiences of differentiation and exclusion. The article engages with the narratives of migrants and city residents, focusing on examples from Zagreb’s Dugave neighborhood. We delve into processes surrounding fencing, policing, spatial segregation, and nightclub bans and highlight that encountering the border plays a crucial role in migrants’ urban lives. Ultimately, the article sheds light on the dynamics of urban bordering and social hostility, revealing how the confluence of policies, social attitudes, and physical barriers shapes migrants’ experiences and decisions within the urban context of a new transit and arrival city in the European border regime.

Laminin γ1-dependent basement membranes are instrumental to ensure proper olfactory placode shape, position and boundary with the brain, as well as olfactory axon development.

Despite recent progress, the complex roles played by the extracellular matrix in development and disease are still far from being fully understood. Here, we took advantage of the zebrafish sly mutation which affects Laminin γ1, a major component of basement membranes, to explore its role in the development of the olfactory system. Following a detailed characterisation of Laminin distribution in the developing olfactory circuit, we analysed basement membrane integrity, olfactory placode and brain morphogenesis, and olfactory axon development in sly mutants, using a combination of immunochemistry, electron microscopy and quantitative live imaging of cell movements and axon behaviours. Our results point to an original and dual contribution of Laminin γ1-dependent basement membranes in organising the border between the olfactory placode and the adjacent brain: they maintain placode shape and position in the face of major brain morphogenetic movements, they establish a robust physical barrier between the two tissues while at the same time allowing the local entry of the sensory axons into the brain and their navigation towards the olfactory bulb. This work thus identifies key roles of Laminin γ1-dependent basement membranes in neuronal tissue morphogenesis and axon development in vivo .

A Review of Urban Planning Approaches to Reduce Air Pollution Exposures.

With only 12% of the human population living in cities meeting the air quality standards set by the WHO guidelines, there is a critical need for coordinated strategies to meet the requirements of a healthy society. One pivotal mechanism for addressing societal expectations on air pollution and human health is to employ strategic modeling within the urban planning process. This review synthesizes research to inform coordinated strategies for a healthy society. Through strategic modeling in urban planning, we seek to uncover integrated solutions that mitigate air pollution, enhance public health, and create sustainable urban environments. Successful urban planning can help reduce air pollution by optimizing city design with regard to transportation systems. As one specific example, ventilation corridors i.e. aim to introduce natural wind into urban areas to improve thermal comfort and air quality, and they can be effective if well-designed and managed. However, physical barriers such as sound walls and vegetation must be carefully selected following design criteria with significant trade-offs that must be modeled quantitatively. These tradeoffs often involve balancing effectiveness, cost, aesthetics, and environmental impact. For instance, sound walls are highly effective at reducing noise, provide immediate impact, and are long-lasting. However, they are expensive to construct, visually unappealing, and may block views and sunlight. To address the costly issue of sound walls, a potential solution is implementing vegetation with a high leaf area index or leaf area density. This alternative is also an effective method for air pollution reduction with varying land-use potential. Ultimately, emission regulations are a key aspect of all such considerations. Given the broad range of developments, concerns, and considerations spanning city management, ventilation corridors, physical barriers, and transportation planning, this review aims to summarize the effect of a range of urban planning methods on air pollution considerations.

New insights into the role of myo-inositol in regulating the skin immune response of grass carp (Ctenopharyngodon idella)

Myo-inositol (MI) is a crucial constituent of the cell membrane and has a pivotal role to play in transmembrane signal transduction, ion channel regulation, and cell transport. Fish skin is a vital mucosal immune organ, and there are few studies that have examined the effect of MI on fish skin.Therefore, the purpose of this study was to investigate the role of dietary MI in regulating the skin immune response of grass carp (Ctenopharyngodon idella). A total of 540 fish (221.33 ± 0.84 g) were fed six diets containing graded levels of MI (27.0, 137.9, 286.8, 438.6, 587.7, and 737.3 mg/kg) for 10 weeks. When the growth test was complete, a 14-day challenge was conducted with Aeromonas hydrophila in each group. Our results indicated that the most effective MI concentrations (approximately 438.6–737.3 mg/kg diet) resulted in the following outcomes: (1) MI reduced the morbidity of grass carp skin and increased the thickness of the dense dermis layer. (2) MI enhanced the physical barrier function of the skin, maybe via increasing antioxidant capacity, inhibiting apoptosis, and improving tight junction-related signaling pathways (nuclear factor erythroid 2-related factor 2, p38 mitogen-activated protein kinase, and myosin light chain kinase). (3) MI enhanced skin immune barrier function, possibly via regulating anti-inflammatory and pro-inflammatory cytokines, as well as the target of rapamycin (TOR) and nuclear factor kappa B (NFκB) pathways. This study led to the following conclusion based on its findings: the MI supplementation strengthened the fish skin barrier function and protected it against Aeromonas hydrophila. The dietary MI requirement in on-growing grass carp was estimated to be 370.90 mg/kg by a broken-line method analysis of skin morbidity.

Mechanical Strain, Temperature, and Misalignment Effects on Data Communication between Piezoceramic Ultrasonic Transducers.

Acoustic waves can be used for wireless telemetry as an alternative to situations where electrical or optical penetrators are unsuitable. However, the response of the ultrasonic transducer can be greatly affected by temperature variations, mechanical deformations, misalignment between transducers, and multiple layers in the propagation zone. Therefore, this work sought to quantify such influences on communication between ultrasonic transducers. The experimental measurements were performed at the frequency where power transfer is maximized. Moreover, there were four experimental models, each with its own performed setup. The ultrasonic transducers are attached to both sides of a 6 mm thick stainless-steel plate for configuring just one barrier. Multiple layers of transducers are attached to the outer side of two plates immersed in an acoustic fluid with a 100 mm thick barrier. In both cases, the S21 parameter was used to quantify the influence of the physical barrier because it correlates with the power flow between ports that return after a given excitation. The results showed that when a maximum deformation of 1250 μm/m was applied, the amplitude of the S21 parameter varied around +0.7 dB. Furthermore, increasing the temperature from 30 to 100 °C slightly affected the S21 (+0.8 dB), but the signal decayed quickly for temperatures beyond 100 °C. Additionally, the ultrasonic communication with a multiple layer was found to occur under misalignment with an intersection area of up to 40%. None of the factors evaluated resulted in insufficient power transfer, except for a large misalignment between the transducers. Such results indicate that this type of communication can be a robust alternative, with a minimum alignment of 40% between transducers and electrical penetrators.

Unveiling the Mysteries of the Blood-Brain Barrier: The Problem of the Brain/spinal Pharmacotherapy.

The most critical issue impeding the development of innovative cerebrospinal medications is the blood-brain barrier (BBB). The BBB limits the ability of most medications to penetrate the brain to the CNS. The BBB structure and functions are summarized, with the physical barrier generated by endothelial tight junctions and the transport barrier formed by transporters within the membrane and vesicular processes. The functions of connected cells, particularly the end feet of astrocytic glial cells, microglia, and pericytes, are described. The drugs that cross the blood brain barrier are explained below along with their mechanisms. Some of the associated conditions and problems are given.

Unveiling the Stability of Encapsulated Pt Catalysts Using Nanocrystals and Atomic Layer Deposition.

Platinum exhibits desirable catalytic properties, but it is scarce and expensive. Optimizing its use in key applications such as emission control catalysis is important to reduce our reliance on such a rare element. Supported Pt nanoparticles (NPs) used in emission control systems deactivate over time because of particle growth in sintering processes. In this work, we shed light on the stability against sintering of Pt NPs supported on and encapsulated in Al2O3 using a combination of nanocrystal catalysts and atomic layer deposition (ALD) techniques. We find that small amounts of alumina overlayers created by ALD on preformed Pt NPs can stabilize supported Pt catalysts, significantly reducing deactivation caused by sintering, as previously observed by others. Combining theoretical and experimental insights, we correlate this behavior to the decreased propensity of oxidized Pt species to undergo Ostwald ripening phenomena because of the physical barrier imposed by the alumina overlayers. Furthermore, we find that highly stable catalysts can present an abundance of under-coordinated Pt sites after restructuring of both Pt particles and alumina overlayers at a high temperature (800 °C) in C3H6 oxidation conditions. The enhanced stability significantly improves the Pt utilization efficiency after accelerated aging treatments, with encapsulated Pt catalysts reaching reaction rates more than two times greater than those of a control supported Pt catalyst.