The marine ecosystem support variety of habitat that consequently supports high species diversity. The present study investigated the diversity of marine macrofauna in the rocky intertidal zone of Diu, Gujarat. Survey was carried out from 4 different sites during April 2022 to March 2023. During the study, all the study sites were surveyed regularly on monthly basis during the lowest low tide period. During the examination, the encountered fauna was carefully observed along the coast using a Quadrat method and visual encounter method and extensive photography was carried out. This study demonstrates 70 species of macrofauna at coastal area of Diu. The highest diversity was observed in the phylum Mollusca (37 species), followed by phylum Arthropoda (16 species), Cnidaria (8 species), Porifera (4 species), Annelida (species 2), Echinodermata (2 species), Platyhelminthes (1 species). This study highlights the importance of protecting the diverse marine habitats in Diu and emphasizes the need for further research on the ecology and conservation of the region’s unique marine biodiversity.