Photon Flux Research Articles

Alleviating Effects of Methyl Jasmonate on Pepper (Capsicum annuum L.) Seedlings under Low-Temperature Combined with Low-Light Stress.

Low temperature combined with low light (LL) is an important factor limiting pepper quality and yield. 'Hang Jiao No. 2' were used as experimental materials, and different concentrations of MeJA (T1 (0 μM), T2 (100 μM), T3 (150 μM), T4 (200 μM), T5 (250 μM) and T6 (300 μM)) were sprayed under LL stress to explore the positive effect of exogenous methyl jasmonate (MeJA) on peppers under LL stress. The photosynthetic properties, osmoregulatory substance, reactive oxygen species, antioxidant enzyme activities, and related gene expressions of the peppers were measured. Our results demonstrated that 200 μM MeJA treatment significantly increased chlorophyll content, light quantum flux per active RC electron transfer (Eto/RC), maximum captured photonic flux per active RC (TRo/RC), energy flux for electron transfer in the excitation cross section (Eto/CSm), energy flux captured by absorption in the excitation cross section (TRo/CSm), soluble protein, and soluble sugar content. Moreover, it significantly improved the maximum photochemical efficiency of PSII (Fv/Fm) and performance index based on absorbed light energy (PI (abs)) by 56.77% and 67.00%, respectively, and significantly decreased malondialdehyde (MDA) content and relative conductivity by 30.55% and 28.17%, respectively. Additionally, antioxidant enzyme activities were elevated, and the expression of the related genes was activated in pepper seedlings under stress, leading to a significant reduction in reactive oxygen species content. In conclusion, our findings confirmed that 200 μM MeJA could reduce the injury of LL to pepper leaves to the photosynthetic organs of pepper leaves, protect the integrity of the cell membrane, and further improve the tolerance of pepper seedlings to LL.

Search for photons above 1018 eV by simultaneously measuring the atmospheric depth and the muon content of air showers at the Pierre Auger Observatory

The Pierre Auger Observatory is the most sensitive instrument to detect photons with energies above 1017 eV. It measures extensive air showers generated by ultrahigh energy cosmic rays using a hybrid technique that exploits the combination of a fluorescence detector with a ground array of particle detectors. The signatures of a photon-induced air shower are a larger atmospheric depth of the shower maximum (Xmax) and a steeper lateral distribution function, along with a lower number of muons with respect to the bulk of hadron-induced cascades. In this work, a new analysis technique in the energy interval between 1 and 30 EeV (1 EeV=1018 eV) has been developed by combining the fluorescence detector-based measurement of Xmax with the specific features of the surface detector signal through a parameter related to the air shower muon content, derived from the universality of the air shower development. No evidence of a statistically significant signal due to photon primaries was found using data collected in about 12 years of operation. Thus, upper bounds to the integral photon flux have been set using a detailed calculation of the detector exposure, in combination with a data-driven background estimation. The derived 95% confidence level upper limits are 0.0403, 0.01113, 0.0035, 0.0023, and 0.0021 km−2 sr−1 yr−1 above 1, 2, 3, 5, and 10 EeV, respectively, leading to the most stringent upper limits on the photon flux in the EeV range. Compared with past results, the upper limits were improved by about 40% for the lowest energy threshold and by a factor 3 above 3 EeV, where no candidates were found and the expected background is negligible. The presented limits can be used to probe the assumptions on chemical composition of ultrahigh energy cosmic rays and allow for the constraint of the mass and lifetime phase space of super-heavy dark matter particles. Published by the American Physical Society 2024

Simultaneous spectral illumination of microplates for high-throughput optogenetics and photobiology.

The biophysical characterization and engineering of optogenetic tools and photobiological systems has been hampered by the lack of efficient methods for spectral illumination of microplates for high-throughput analysis ofaction spectra. Current methods to determine action spectraonly allow the sequential spectral illumination of individual wells. Here we present the open-source RainbowCap-system, which combines LEDs and optical filters in a standard 96-well microplate format for simultaneous and spectrally defined illumination. The RainbowCap provides equal photon flux for each wavelength, with the output of the LEDs narrowed by optical bandpass filters. We validatedthe RainbowCap for photoactivatable G protein-coupled receptors (opto-GPCRs) and enzymes for the control of intracellular downstream signaling. The simultaneous, spectrally defined illumination provides minimal interruption during time-series measurements, while resolving 10 nm differences in the action spectra of optogenetic proteins under identical experimental conditions. The RainbowCap is also suitable for studying the spectral dependence of light-regulated gene expression in bacteria, which requires illumination over several hours. In summary, the RainbowCap provides high-throughput spectral illumination of microplates, while its modular, customizable design allows easy adaptation to a wide range of optogenetic and photobiological applications.

Effects of turfgrass canopy shade levels and quantum spectrum on the germination and development of smooth crabgrass (Digitaria ischaemum)

AbstractSmooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. ex Muhl.] is one of the most troublesome summer annual weeds in cool‐season turfgrass. Field experiments demonstrated a strong relationship between spring photosynthetic active radiation reaching the soil surface and the resulting smooth crabgrass cover in Kentucky bluegrass (Poa pratensis L.) 2/3 months later in summer. Both smooth crabgrass and canopy penetrating light were consistently reduced by higher mowing heights. Follow‐up experiments in controlled environments were initiated to improve our understanding of how light quality and quantity influence smooth crabgrass germination and growth. Smooth crabgrass germination was ≥99% following exposure to blue, red (R), far red (FR), both R and FR light pulses, or complete darkness treatments. A second germination experiment examined six levels of turfgrass canopy shade (0%, 24%, 44%, 77%, 90%, and 100% photosynthetic photon flux density [PPFD] reduction) and found that smooth crabgrass germination was ≥99% for all treatments. These experiments indicate that light quality and quantity do not affect smooth crabgrass germination. A greenhouse experiment examined five levels of turfgrass canopy shade (0%, 44%, 59%, 81%, and 91% PPFD reduction). Smooth crabgrass quantum and PSII (Photosystem II) operating efficiency increased in response to shade, while leaf number and thickness, specific leaf weight, tillering, mass, electron transport rate, and stomatal conductance decreased with increasing shade. Overall, the results demonstrate that smooth crabgrass is able to germinate regardless of canopy density, but seedling growth, development, and plant function are diminished in a dense turfgrass canopy shade.

Understanding Rhododendron intraspecific compatibility in botanic garden collections for species conservation

Context Controlled pollination is an important technique for maintaining intraspecific diversity in integrated plant conservation practices, particularly in genera such as Rhododendron, where open pollination usually produces hybrids with unknown paternal lineages. Aims This study investigated the capacity for viable seed set from self- and intraspecific cross-pollination for Rhododendron taxa in different categories of the International Union for Conservation of Nature (IUCN) Red List, to guide conservation management of threatened species in botanic garden collections. Methods The following five taxa of subsection Maddenia were studied: R. dalhousiae var. dalhousiae (Least Concern), R. dalhousiae var. rhabdotum (Vulnerable), R. lindleyi (Least Concern), R. nuttallii (Near Threatened), and R. excellens (Vulnerable). Controlled pollination was performed on selected garden accessions, and seed germination was tested at an alternating temperature regime of 15/25°C, 8 h photoperiod, and ~6 μmol m−2 s−1 photosynthetic photon flux density (PPFD). Key results Intraspecific compatibilities varied among different taxa and between self- and outcross treatments. X-ray images for Rhododendron seeds showed low capacity to predict seed germination. Neither X-ray scan nor fungicide (Ridomil) treatment showed any adverse impact on seed germination, which has positive implications for seed-banking and subsequent raising of Rhododendron seedlings. Conclusions Controlled intraspecific pollination can be used to maintain diversity of ex situ accessions for selected Rhododendron species. However, the zero or low compatibility demonstrated in some species, such as R. excellens, suggests that these species may require a different approach. Implications Intraspecific pollination should be evaluated for each Rhododendron species before a propagation program is initiated in ex situ conservation.

TAG-SPARK: Empowering High-Speed Volumetric Imaging With Deep Learning and Spatial Redundancy.

Two-photon high-speed fluorescence calcium imaging stands as a mainstream technique in neuroscience for capturing neural activities with high spatiotemporal resolution. However, challenges arise from the inherent tradeoff between acquisition speed and image quality, grappling with a low signal-to-noise ratio (SNR) due to limited signal photon flux. Here, a contrast-enhanced video-rate volumetric system, integrating a tunable acoustic gradient (TAG) lens-based high-speed microscopy with a TAG-SPARK denoising algorithm is demonstrated. The former facilitates high-speed dense z-sampling at sub-micrometer-scale intervals, allowing the latter to exploit the spatial redundancy of z-slices for self-supervised model training. This spatial redundancy-based approach, tailored for 4D (xyzt) dataset, not only achieves >700% SNR enhancement but also retains fast-spiking functional profiles of neuronal activities. High-speed plus high-quality images are exemplified by in vivo Purkinje cells calcium observation, revealing intriguing dendritic-to-somatic signal convolution, i.e., similar dendritic signals lead to reverse somatic responses. This tailored technique allows for capturing neuronal activities with high SNR, thus advancing the fundamental comprehension of neuronal transduction pathways within 3D neuronal architecture.

Long-term carrier lifetime instabilities in n-type FZ- and Cz-silicon under illumination at elevated temperature

Even though n-type Si wafers show a lower extent of light-induced degradation, similar degradation and regeneration behavior as in p-type Si materials has been observed for various n-type Si monocrystalline materials. In this work, the long-term behavior of minority charge carrier lifetime in non-diffused n-type FZ-Si and Cz-Si wafers during illumination at elevated temperatures is investigated. Thereby, various influencing factors such as peak firing temperature and treatment temperature are considered. Degradation and a very strong regeneration effect, which exceeds the initial effective lifetime value, can be observed in the samples. Since surface-related saturation current density remains almost constant during degradation and regeneration, the effect seems to be bulk related. The extent of degradation and regeneration could be shown to be firing temperature-dependent. It could also be shown for n-type FZ-Si wafers that higher firing temperatures result in an increased H2 content within the silicon bulk. Investigations of the injection-dependent defect density revealed that the defect formed during degradation is not a deep level Shockley-Read-Hall defect. Observations at different treatment temperatures and constant illumination of 0.9(1) sun photon flux equivalent have shown that a higher treatment temperature leads to faster degradation and regeneration kinetics. Similarly, higher injection rates also speed up regeneration kinetics. Since degradation also occurs in darkness, the bulk related degradation effect in n-type FZ-Si is charge carrier-induced and not necessarily light-induced.

Prolonged Post-Harvest Preservation in Lettuce (Lactuca sativa L.) by Reducing Water Loss Rate and Chlorophyll Degradation Regulated through Lighting Direction-Induced Morphophysiological Improvements.

To investigate the relationship between the lighting direction-induced morphophysiological traits and post-harvest storage of lettuce, the effects of different lighting directions (top, T; top + side, TS; top + bottom, TB; side + bottom, SB; and top + side + bottom, TSB; the light from different directions for a sum of light intensity of 600 μmol·m-2·s-1 photosynthetic photon flux density (PPFD)) on the growth morphology, root development, leaf thickness, stomatal density, chlorophyll concentration, photosynthesis, and chlorophyll fluorescence, as well as the content of nutrition such as carbohydrates and soluble proteins in lettuce were analyzed. Subsequently, the changes in water loss rate, membrane permeability (measured as relative conductivity and malondialdehyde (MDA) content), brittleness (assessed by both brittleness index and β-galactosidase (β-GAL) activity), and yellowing degree (evaluated based on chlorophyll content, and activities of chlorophyllase (CLH) and pheophytinase (PPH)) were investigated during the storage after harvest. The findings indicate that the TS treatment can effectively reduce shoot height, increase crown width, enhance leaves' length, width, number, and thickness, and improve chlorophyll fluorescence characteristics, photosynthetic capacity, and nutrient content in lettuce before harvest. Specifically, lettuce's leaf thickness and stomatal density showed a significant increase. Reasonable regulation of water loss in post-harvested lettuce is essential for delaying chlorophyll degradation. It was utilized to mitigate the increase in conductivity and hinder the accumulation of MDA in lettuce. The softening speed of leafy vegetables was delayed by effectively regulating the activity of the β-GAL. Chlorophyll degradation was alleviated by affecting CLH and PPH activities. This provides a theoretical basis for investigating the relationship between creating a favorable light environment and enhancing the post-harvest preservation of leafy vegetables, thus prolonging their post-harvest storage period through optimization of their morphophysiological phenotypes.

Investigating the influence of varied ratios of red and far-red light on lettuce (Lactuca sativa): effects on growth, photosynthetic characteristics and chlorophyll fluorescence.

Far red photon flux accelerates photosynthetic electron transfer rates through photosynthetic pigments, influencing various biological processes. In this study, we investigated the impact of differing red and far-red light ratios on plant growth using LED lamps with different wavelengths and Ca1.8Mg1.2Al2Ge3O12:0.03Cr3+ phosphor materials. The control group (CK) consisted of a plant growth special lamp with 450 nm blue light + 650 nm red light. Four treatments were established: F1 (650 nm red light), F2 (CK + 730 nm far-red light in a 3:2 ratio), F3 (650 nm red light + 730 nm far-red light in a 3:2 ratio), and F4 (CK + phosphor-converted far-red LED in a 3:2 ratio). The study assessed changes in red and far-red light ratios and their impact on the growth morphology, photosynthetic characteristics, fluorescence characteristics, stomatal status, and nutritional quality of cream lettuce. The results revealed that the F3 light treatment exhibited superior growth characteristics and quality compared to the CK treatment. Notably, leaf area, aboveground fresh weight, vitamin C content, and total soluble sugar significantly increased. Additionally, the addition of far-red light resulted in an increase in stomatal density and size, and the F3 treatments were accompanied by increases in net photosynthetic rate (Pn), transpiration rate (Tr), intercellular CO2 concentration (Ci), and stomatal conductance (Gs). The results demonstrated that the F3 treatment, with its optimal red-to-far-red light ratio, promoted plant growth and photosynthetic characteristics. This indicates its suitability for supplementing artificial light sources in plant factories and greenhouses.

Cancer radiotherapy with mini neutron/gamma-ray generators

Recent advancements in negative hydrogen (H-) or negative deuterium (D-) ion source technology as well as the commercial availability of high-frequency AC high-voltage power supplies have enabled the development of mini neutron/gamma-ray generators using low-energy nuclear reactions. These generators can provide a useful flux of high-energy neutrons or gamma photons in either pulsed or continuous operations. With the new mini generator, intraoperative radiotherapy as well as treatment of tumors in the brain, skin, breast, salivary gland, pancreas, liver, and kidney can be performed using external or internal neutron/gamma-ray beams. The new radiotherapy system is very compact and requires very low power for operation, enabling its location inside an operation room of a hospital or clinical facility.

Galactic Stellar Black Hole Binaries: Spin Effects on Jet Emissions of High-Energy Gamma-Rays

In the last few decades, galactic stellar black hole X-ray binary systems (BHXRBs) have aroused intense observational and theoretical research efforts specifically focusing on their multi-messenger emissions (radio waves, X-rays, γ-rays, neutrinos, etc.). In this work, we investigate jet emissions of high-energy neutrinos and gamma-rays created through several hadronic and leptonic processes taking place within the jets. We pay special attention to the effect of the black hole’s spin (Kerr black holes) on the differential fluxes of photons originating from synchrotron emission and inverse Compton scattering and specifically on their absorption due to the accretion disk’s black-body radiation. The black hole’s spin (dimensionless spin parameter a*) enters into the calculations through the radius of the innermost circular orbit around the black hole, the RISCO parameter, assumed to be the inner radius of the accretion disk, which determines its optical depth τdisk. In our results, the differential photon fluxes after the absorption effect are depicted as a function of the photon energy in the range 1GeV ≤E≤103GeV. It is worth noting that when the black holes’ spin (α*) increases, the differential photon flux becomes significantly lower.

The Effect of Light Intensity during Cultivation and Postharvest Storage on Mustard and Kale Microgreen Quality.

Microgreens are vegetable greens that are harvested early while they are still immature and have just developed cotyledons. One of the disadvantages and a challenge in production is that they exhibit a short shelf life and may be damaged easily. In seeking to prolong the shelf life, some pre- and postharvest interventions have been investigated. Here, kale and mustard microgreens were grown in a controlled-environment walk-in chamber at +21/17 °C, with ~65% relative air humidity, while maintaining the spectral composition of deep red 61%, blue 20%, white 15%, and far red 4% (150, 200, and 250 µmol m-2 s-1 photosynthetic photon flux density (PPFD)). Both microgreens seemed to exhibit specific and species-dependent responses. Higher PPFD during growth and storage in light conditions resulted in increased contents of TPC in both microgreens on D5. Additionally, 150 and 250 PPFD irradiation affected the α-tocopherol content by increasing it during postharvest storage in kale. On D0 150 for kale and 200 PPFD for mustard microgreens, β-carotene content increased. D5 for kale showed insignificant differences, while mustard responded with the highest β-carotene content, under 150 PPFD. Our findings suggest that both microgreens show beneficial outcomes when stored in light compared to dark and that mild photostress is a promising tool for nutritional value improvement and shelf-life prolongation.

Search for MeV gamma-ray emission from TeV bright red dwarfs with COMPTEL

The SHALON atmospheric Cherenkov telescope has detected very high energy gamma-ray emission at TeV energies from eight red dwarfs, namely, V388 Cas, V547 Cas, V780 Tau, V962 Tau, V1589 Cyg, GJ 1078, GJ 3684 and GL 851.1. Consequently, these red dwarfs have been suggested as sources of ultra-high energy cosmic rays. In this work, we search for soft gamma-ray emission from these TeV bright red dwarfs between 0.75–30 MeV using archival data from the COMPTEL gamma-ray imaging telescope, as a follow-up to a similar search for GeV gamma-ray emission using the Fermi-LAT telescope. Although, prima-facie, we detect non-zero photon flux from three red dwarfs with high significance, these signals can attributed to contamination from nearby sources such as Crab and Cygnus, which are within the angular resolution of COMPTEL, and have been previously detected as very bright point sources at MeV energies. Therefore, we could not detect any statistically significant signal (> 3σ) from any of these eight red dwarfs from 0.75–30 MeV. We then report the 95% confidence level upper limits on the differential photon flux (at 30 MeV), integral photon flux and integral energy flux for all of the eight red dwarfs. The integral energy flux limits range between 10-11 - 10-10-ergs/cm2/s.

Numerical investigation of vacuum ultra-violet emission in Ar/O2 inductively coupled plasmas

Abstract Controlling fluxes of vacuum ultraviolet (VUV) radiation is important in a number of industrial and biomedical applications of low pressure plasma sources because, depending on the process, VUV radiation may be desired, required to a certain degree, or unwanted. In this work, the emission of VUV radiation from O atoms is investigated in low-pressure Ar/O2 inductively coupled plasmas via numerical simulations. For this purpose, a self-consistent Ar/O2 plasma-chemical reaction scheme has been implemented in a zero dimensional plasma chemical kinetics model and is used to investigate VUV emission from excited O atoms (3s 5S 2 0 and 3s 3S 1 0 ) at 130 and 135 nm. The model is extensively compared with experimental measurements of absolute VUV emission intensities, electron densities and Ar excited state densities. In addition, oxygen VUV emission intensities are investigated as a function of pressure, Ar/O2 mixture, and power deposition and the dominant reaction pathways leading to oxygen VUV emission are identified and described. In general terms, absolute oxygen VUV emission intensities increase with power and oxygen fraction over the ranges investigated and peak emission intensities are found for pressures between 5–50 Pa. The emission is dominated by the 130 nm resonance line from the decay of the O(3s 3S 1 0 ) state to the ground state. Besides, at low pressure (0.3–1 Pa), the flux of oxygen VUV photons to surfaces is much lower than that of positive ions, whereas oxygen VUV fluxes dominate at higher pressure, ≳ 5–50 Pa depending on O2 fraction. Finally, oxygen atom fluxes to surfaces are, in general, larger than those of VUV photons for the parameter space investigated.

X-ray polarisation in AGN circumnuclear media

Context. Cold gas and dust reprocess the central X-ray emission of active galactic nuclei (AGN), producing characteristic spectro-polarimetric features in the X-ray band. The recent launch of IXPE allows for observations of this X-ray polarisation signal, which encodes unique information on the parsec-scale circumnuclear medium of obscured AGN. However, the models for interpreting these polarimetric data are under-explored and do not reach the same level of sophistication as the corresponding spectral models. Aims. We aim at closing the gap between the spectral and spectro-polarimetric modelling of AGN circumnuclear media in the X-ray band by providing the tools for simulating X-ray polarisation in complex geometries of cold gas alongside X-ray spectra. Methods. We lay out the framework for X-ray polarisation in 3D radiative transfer simulations and provide an implementation to the 3D radiative transfer code SKIRT, focussing on (de)polarisation due to scattering and fluorescent re-emission. As an application, we explored the spectro-polarimetric properties of a 2D toroidal reprocessor of cold gas, modelling the circumnuclear medium of AGN. Results. For the 2D torus model, we find a complex behaviour of the polarisation angle with photon energy, which we interpret as a balance between the reprocessed photon flux originating from different sky regions, with a direct link to the torus geometry. We calculated a large grid of AGN torus models and demonstrated how spatially resolved X-ray polarisation maps could form a useful tool for interpreting the geometrical information that is encoded in IXPE observations. With this work, we release high-resolution AGN torus templates that simultaneously describe X-ray spectra and spectro-polarimetry for observational data fitting with XSPEC. Conclusions. The SKIRT code can now model X-ray polarisation simultaneously with X-ray spectra and provide synthetic spectro-polarimetric observations for complex 3D circumnuclear media, with all features of the established SKIRT framework available.

Ultrafast energy-dispersive soft-x-ray diffraction in the water window with a laser-driven source

Time-resolved soft-x-ray-diffraction experiments give access to microscopic processes in a broad range of solid-state materials by probing ultrafast dynamics of ordering phenomena. While laboratory-based high-harmonic generation (HHG) light sources provide the required photon energies, their limited photon flux is distributed over a wide spectral range, rendering typical monochromatic diffraction schemes challenging. Here, we present a scheme for energy-dispersive soft-x-ray diffraction with femtosecond temporal resolution and photon energies across the water window from 200 to 600 eV. The experiment utilizes the broadband nature of the HHG emission to efficiently probe large slices in reciprocal space. As a proof-of-concept, we study the laser-induced structural dynamics of a Mo/Si superlattice in an ultrafast, non-resonant soft-x-ray diffraction experiment. We extract the underlying strain dynamics from the measured shift of its first order superlattice Bragg peak in reciprocal space at photon energies around 500 eV via soft-x-ray scattering simulations.

Microbial biofertilizers and algae-based biostimulant affect fruit yield characteristics of organic processing tomato.

Microbial biofertilizers and algae-based biostimulants have been recognized for supporting sustainable agriculture. Field experiments were conducted in 2022 and 2023 growing seasons in an organic farm located in Ferrara (Italy) with the aim of evaluating plant growth-promoting microorganisms (PGPMs) and algae-based biostimulants (Biost) in tomato (Solanum lycopersicum L.). The experimental treatments were: (i) two microbial biofertilizers (PGPM_1, PGPM_2) and no inoculated plants (No_PGPM); and (ii) two algae-based biostimulant rates (0.5% (Biost_0.5%), 1.0% (Biost_1.0%)) and no application (No_Biost). PGPMs were applied at transplanting, while biostimulants at 15 and 30 days after transplanting. Treatments were replicated three times according to a split-plot experimental design. Plant characteristics were evaluated at 30 days after transplanting in No_Biost treatments. During tomato cultivation, soil plant analysis development (SPAD), nitrogen difference vegetation index (NDVI), leaf area index (LAI) and photosynthetic photon flux density (PPFD) were monitored. Tomato yield was determined. PGPM_2 showed the highest shoot biomass (132.9 g plant-1), plant height (44.7 cm), leaf number (34.0 plant-1) and root biomass (9.22 g plant-1). Intermediate values were observed in PGPM_1, while all parameters were lower in No_PGPM. Both PGPMs achieved higher values of SPAD, NDVI, PPFD and LAI than No_PGPM. Biost_1.0% increased all measured growth parameters followed by Biost_0.5% and No_Biost, respectively. Tomato yield was the highest for PGPM_2-Biost_1.0% (67.2 t ha-1). PGPMs affected fruit size and sugar content, while biostimulants were associated with color and lycopene. The application of microbial biofertilizers and algae-based biostimulants could be part of environment-friendly practice in organic farming. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Varying Light Intensities Affect Lettuce Growth and Physiology in Controlled Indoor Environments

Agriculture in controlled environments has gained popularity over time. Compared to traditional agriculture, controlled environments emerge as an alternative to mitigate the negative impacts of conventional farming methods. However, controlled environment agriculture, particularly plant factories with artificial lighting, incurs higher electricity costs, primarily for supplemental lighting and dehumidification of the cultivation area. Given these high costs, it is crucial to understand how efficiently plants utilize available light to convert it into biomass. This understanding can be used to design lighting strategies to reduce electricity usage. In this study, we cultivated ‘Rex’ lettuce (Lactuca sativa) plants on a soilless substrate and used an ebb-and-flow system for irrigation and fertilization. Plants grew in varying photosynthetic photon flux density (PPFD) levels ranging from 125 to 375 µmol·m−2·s−1 and were assessed for various physiological responses. Our findings revealed that plants exposed to higher light levels exhibited greater final dry weight, increased photosynthetic activity, higher water use efficiency, and accelerated growth compared to those under lower light conditions. Notably, plants subjected to higher light intensities did not show a significant increase in transpiration, suggesting a potential trade-off between energy expenditure on supplemental lighting and dehumidification. This finding opens the possibility of reducing energy consumption for dehumidification and achieving economic savings by subjecting plants to optimal growing conditions for shorter durations. This depends on whether higher savings on dehumidification are achieved compared to the energy required to maintain high PPFD levels.

Species-specific effects of light and temperature on photosynthesis and respiration among Symbiodiniaceae (Dinophyceae)

Coral reefs are exposed to various environmental stressors that cause bleaching events, whereby endosymbiotic microalgae (Symbiodiniaceae) disassociate from coral hosts. Bleached corals are compromised and face mortality. The combination of high-light exposure and elevated seawater temperature often lead to coral bleaching. The physiological properties of the Symbiodiniaceae within the coral tissues contribute to the thermal tolerance of the holobiont (the host and all its symbionts). The present study aimed to investigate the effects of light and temperature stress on four Symbiodiniaceae species from three genera with respect to photosynthetic oxygen production and consumption. Under control conditions, the species displayed predominantly low-to-moderate light requirements for photosynthesis with increased photoinhibition at higher photon flux rates. After 30 days of heat acclimation at 32 °C, maximum photosynthetic activity declined in Effrenium voratum, doubled in Fugacium kawagutii, and remained unchanged in Breviolum psygmophilum. In subsequent acute heating assays, species-specific effects on maximum photosynthetic activity were observed. Photosynthesis in all species declined across a temperature gradient between 25 and 39 °C in the acute heating assays; full inhibition occurred at 37 °C in B. psygmophilum and E. voratum and at 39 °C in B. aenigmaticum and F. kawagutii. In contrast, respiration remained largely constant in all species across temperatures. Our data point to species-specific photophysiological traits that lead to different thermal tolerances among Symbiodiniaceae.

Nitrite sensitized photolysis of ibuprofen in the liquid and ice phases: Roles of reactive species and influences of the main dissolved components

This study evaluated the kinetics, mechanisms, toxicity assessment, and influencing factors of ibuprofen (IBP) photolysis induced by nitrite (NO2–) in the liquid and ice phases. The photon-flux-normalized rate constant for IBP photolysis (kIBP*) increased monotonically with increasing NO2– concentrations in the liquid and ice phases. kIBP* in the ice phase was decreased by 21.5–29.9 %, as compared with those obtained with the same NO2– concentration in the liquid phase. Reactive nitrogen species (RNS) contributed more to NO2–-sensitized photolysis of IBP than hydroxyl radicals (•OH) in the liquid and ice phases, and the contributions of RNS to IBP degradation in the ice phase were higher than those in the liquid phase. The NO2–-sensitized photolysis mechanisms of IBP in the ice phase seemed to be similar to those in the liquid phase, including hydroxylation, nitration, nitrosation, decarboxylation, side-chain cleavage and ketonization. The reactions of IBP with RNS were more favored in the ice phase due to higher photon flux, leading to greater generation of photoproducts with higher toxicity. Bicarbonate (HCO3–) inhibited NO2–-sensitized photolysis of IBP. Although dissolved organic matter (DOM) itself could induce IBP photolysis, it exhibited an inhibitory effect on NO2–-sensitized photolysis of IBP. For the real water and ice samples containing low NO2– concentrations (≤ 3.6 μM) and medium DOM concentrations (2.6–5.1 mg C/L), DOM played a more important role in IBP photolysis than NO2– in the liquid phase and particularly in the ice phase. kIBP* for the real samples in the ice phase were 32.1–136.4 % higher than those in the liquid phase.